Anti-DNA helicase B (HELB)

eCl@ss:

32160702

Кат. номер

ABE2601-25UG

ABE2601-100UG

DNA helicase B (UniProt: Q8NG08; also known as EC: 3.6.4.12, hDHB) is encoded by the HELB gene (Gene ID: 92797) in human. DNA helicase B is highly expressed in testis and thymus and weaker expression is observed in liver, spleen, kidney, and brain. It is predominantly nuclear, however, following phosphorylation at serine 967 by CDK2 during the G1/S transition it is exported into the cytoplasm. Following DNA damage, it is recruited to sites of double-strand breaks by the RPA complex. Its recruitment to single-stranded DNA following DNA damage leads to inhibition of nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5-3 ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following its phosphorylation. Its nuclear export signal is localized to amino acids 1055-1078. It uses either magnesium or manganese ions to support its helicase activity and the DNA helicase B activity is inhibited by salt concentrations higher than 100 mM. It binds strongly to single-stranded DNA in the absence of ATP, but dissociates readily in the presence of 1 mM ATP. Two isoforms of DNA helicase B have been reported that are produced by alternative splicing.
Specificity
This rabbit polyclonal antibody specifically detects DNA helicase B in human cells.
MBP-conjugated recombinant fragment corresponding to the first 199 amino acids from the N-terminal region of human DNA helicase B.
Epitope: N-terminus
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected DNA helicase B (HELB) in U2OS cells transfected with a non-targeting siRNA (CTRL) or siRNAs targeting protein and HeLa cells transfected with non-targeting (siCTRL) or HELB-targeting (siHELB) siRNAs (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).
Anti-DNA helicase B (HELB) Antibody, Cat. No. ABE2601, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting in siRNA control & siRNA HELB U2OS cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected DNA helicase B (HELB) in lysate from U2OS cells treated with non-targeting siRNA, but not in lysates from siRNA HELB treated U2OS cells.
Target description
~145 kDa observed; 123.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG
NCBI accession no.	NP_387467,
UniProt accession no.	Q8NG08,