Anti-Delta-like protein 1 Antibody, clone PGPM-1F9

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABN2284

MABN2284-25UG

Delta-like protein 1 (UniProt: Q61483; also known as Drosophila Delta homolog 1, Delta1, DLL1) is encoded by the Dll1 gene (Gene ID: 13388) in murine species. DLL1 is a single-pass transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner. Following transinteraction, ligand cells produce mechanical force that promotes Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling. Delta1 is required for embryonic development and maintenance of adult stem cells in many different tissues. DLL1-induced Notch signaling plays a role in the regulation of cell lineage, cell specification, cell patterning, and morphogenesis through effects on differentiation and proliferation. DLL1 is reported to play a role in regulating neuronal differentiation of neural precursor cells via cell-cell interaction. DLL1 is reported to be co-expressed with DLL3 in the presomtic mesoderm of mouse embryo, however despite their co-expression, mutations in these proteins cause strikingly different effects. Heterozygous DLL1 mutant mice are lighter and smaller, with altered fat to lean ratio and display increased blood pressure and a slight bradycardia. They also exhibit reduced cholesterol and triglyceride levels in blood.
Specificity
Clone PGPM-1F9 specifically detects murine Delta Like protein 1. It targets an epitope within 17 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of murine Delta-like protein 1.
Anti-Delta-like protein 1 antibody, clone PGPM-1F9, Cat. No. MABN2284, is a highly specific rat monoclonal antibody that targets Delta-like protein 1 and has been tested in Flow Cytometry, Immunohistochemistry, and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Delta-like protein 1 in Immunohistochemistry applications (Bone, R.A., et. al. (2014). Development. 141(24):4806-16).
Flow Cytometry Analysis: 1 µg from a representative lot detected Delta-like protein 1 in one million DLL1-flag A7A1/CHO cells expressing DLL1.
Western Blotting Analysis: A representative lot detected Delta-like protein 1 in Western Blotting applications (Geffers, I., et. al. (2007). J Cell Biol. 178(3):465-76).
Research Category
Neuroscience
Quality
Evaluated by Western Blotting in DLL1-FLAG/CHO cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Delta-like protein 1 in 10 µg of lysate from CHO cells stably expressing Flag-tagged Delta-like Protein 1.
Target description
~78 kDa observed; 78.45 calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	PGPM-1F9, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_031891,
UniProt accession no.	Q61483,
shipped in	ambient