Anti-Cyclin D2 Antibody, clone D2-34B1-3

Кат. номер

MABE1988-25UG

MABE1988-100UG

G1/S-specific cyclin-D2 (UniProt: P30280; also known and Cyclin D2;CCND2) is encoded by the Ccnd2 (also known as Cyl-2) gene (Gene ID: 12444) in murine species. Cell cycle progression is regulated by the sequential events that include activation and subsequent inactivation of cyclin dependent kinases (Cdk) and cyclins. Cyclins impart substrate specificity to the CDK/cyclin complex. D-type cyclins (D1, D2, and D3) are known to have critical roles in cell cycle progression, differentiation, and malignant transformation. They are encoded by distinct genes but show significant amino-acid similarity. D-type cyclins are shown to associate with cyclin-dependent kinase 4 and 6 (CDK4, CDK6) and promote phosphorylation and subsequent inactivation of the retinoblastoma tumor suppressor gene product, RB and RB-related proteins. Phosphorylation of RB allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase.
Specificity
Clone D2-34B1-3 is a rat monoclonal antibody that detects Cyclin D2.
GST-tagged recombinant murine Cyclin D2.
Quality Control Testing
Evaluated by Western Blotting in Mouse spleen tissue lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cyclin D2 in Mouse spleen tissue lysates.
Tested Applications
Immunofluorescence Analysis: A representative lot detected Cyclin D2 in Immunofluorescence applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).
Western Blotting Analysis: A representative lot detected Cyclin D2 in Western Blotting applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cyclin D2 in Immunoprecipitation applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).
Immunohistochemistry Applications: A representative lot detected Cyclin D2 in Immunohistochemistry applications (Rowan, S., et al. (2008). Dev Biol.;321(1):111-22).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Cyclin D2, clone D2-34B1-3, Cat. No. MABE1988, is a rat monoclonal antibody that detects G1/S-specific cyclin-D2 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	D2-34B1-3, monoclonal
mol wt	calculated mol wt 32.90 kDa","observed mol wt ~28 kDa
purified by	using protein G
species reactivity	mouse, human
packaging	antibody small pack of 100 µg
application(s)	immunofluorescence: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2a
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_033959,
UniProt accession no.	P30280,
shipped in	2-8°C
Gene Information	mouse ... Ccnd2(12444)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable