Anti-CTCF Antibody

eCl@ss:

32160702

Кат. номер

7-729

7-729-25UL

7-729-100UL

CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
Specificity
Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
Recognizes CTCF at a.a. 659-675.
KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
Chromatin Immunoprecipitation:
An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
Quality
Routinely evaluated by Western Blot.
Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
Target description
Approx. 120 kDa
Replaces: MABE547
Physical form
Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
HeLa nuclear extract.
Included Positive Antigen Control:
Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
Concentration: The concentration of this serum antibody has not been determined.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source	rabbit
Quality Level	100
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human, mouse, primate, rat, canine
packaging	antibody small pack of 25 µL
Торговая марка	Upstate®
application(s)	ChIP: suitable (ChIP-seq)","western blot: suitable
NCBI accession no.	NM_006565.2,
UniProt accession no.	P49711,
shipped in	ambient
Gene Information	human ... CTCF(10664)