Anti-CHMP3

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

ABS1668

ABS1668-25UL

Charged multivesicular body protein 3 (UniProt: Q9Y3E7; also known as Chromatin-modifying protein 3, CHMP3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24) is encoded by the CHMP3 (also known as CGI149, NEDF, VPS24, CGI-149) gene (Gene ID: 51652) in human. CHMP3 is a component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It is widely expressed and its expression is reported in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The acidic C-terminus and the basic N-terminus of CHMP3 are thought to render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP3 is shown to heteromerize with CHMP2A to form helical tubular structures that expose membrane-interacting sites on the outside. It can also interact with CHMP4A, however, the interaction requires the release of CHMP4A auto-inhibition.
Specificity
This rabbit polyclonal antibody detects Charged multivesicular body protein 3 in human cells.
A full-length human recombinant charged multivesicular body protein 3 (CHMP3).
Epitope: unknown
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant charged multivesicular body protein 3 (CHMP3).
Western Blotting Analysis: A representative lot detected CHMP3 in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94).
Research Category
Signaling
Anti-CHMP3, Cat. No. ABS1668, is a rabbit polyclonal antibody that detects Charged multivesicular body protein 3 and has been tested for use in Immunocytochemistry and Western Blotting.
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CHMP3 in HeLa cells.
Target description
~32 kDa observed; 25.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	serum
antibody product type	primary antibodies
clone	polyclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG
NCBI accession no.	NP_057163,
UniProt accession no.	Q9Y3E7,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable