Anti-CFDP1 Antibody, clone 15E5.1

eCl@ss:

32160702

Кат. номер

MABN396

MABN396-25UL

Craniofacial development protein 1 (UniProt: Q9UEE9; also known as Bucentaur) is encoded by the CFDP1 (also known as BCNT, CENP-29) gene (Gene ID: 10428) in human. Craniofacial development protein 1 is an evolutionarily conserved member of the Bucentaur (BCNT) family of proteins and displays ubiquitous expression. It contains a BCNT domain of about 80 amino acids in the C-terminal region. Two isoforms of craniofacial development protein 1 are reported that are generated by alternative splicing. It is reported to play a role in embryogenesis and is considered as essential for chromatin/chromosome organization and function. A unique feature of this protein is that it lacks cysteine residues and has an acidic N-terminal region and a lysine/glutamic acid/proline-rich region of 40 amino acids. It also has seven serine phosphorylation sites and Ser250 in the BCNT region is shown to be heavily phosphorylated. (Ref.: Messina, G., et al. (2015). Chromosoma 124(2):153 162; Iwashita, S et al. (2015). Biosci Rep. 35(4): e00228).
Specificity
Clone 15E5.1 detects Craniofacial development protein 1 in human. It targets an epitope within the first 111 amino acids from the N-terminal half.
GST-taged recombinant fragment corresponding to the first 111 amino acids from the N-terminal region.
Research Category
Neuroscience
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFDP1 in 10 µg of Jurkat cell lysate.
Anti-CFDP1, clone 15E5.1, Cat. No. MABN396, is a highly specific mouse monoclonal antibody that targets CFDP1 and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFDP1 in 10 µg of HEK293 cell lysate.
Target description
~45 kDa observed; 33.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	15E5.1, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	western blot: suitable
isotype	IgG1
NCBI accession no.	NP_006315,
UniProt accession no.	Q9UEE9,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable