Anti-CD6 Antibody, clone 34-3

eCl@ss:

32160702

Кат. номер

MABF2091-25UG

MABF2091-100UG

T-cell differentiation antigen CD6 (UniProt: Q61003; also known as CD6) is encoded by the Cd6 gene (Gene ID: 12511) in murine species. CD6 is a single-pass type I membrane protein that is detected at the immunological synapse the contact zone between antigen-presenting dendritic cells and T-cells. It functions as a costimulatory molecule and promotes T-cell activation and proliferation. After T-cell activation, CD6 is hyperphosphorylated on serine and threonine residues and it is also phosphorylated on tyrosine residues in response to stimulation of the TCR complex. CD6 is predominantly expressed in thymus, lymph node, and spleen. It mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It also contributes to signaling cascades triggered by activation of the TCR/CD3 complex. It functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. It binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. It mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 is synthesized with a signal peptide (aa 1-16), which is cleaved off to produce a mature form, which has an extracellular domain (aa 17-398), a transmembrane region (aa 399-419), and a long cytoplasmic tail (aa 420-665).
Specificity
Clone 34-3 is a rat monoclonal antibody that detects T-cell differentiation antigen CD6 in murine species.
Epitope: extracellular domain
CD6-transfected NB2 cells
Anti-CD6, clone 34-3, Cat. No. MABF2091, is a rat monoclonal antibody that detecs T-cell differentiation antigen CD6 and has been tested for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Quality
Evaluated by Flow Cytometry in mouse splenocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD6 in one million mouse splenocytes.
Target description
72.26 kDa calculated.
Physical form
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	34-3, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable
isotype	IgG2b
NCBI accession no.	NP_001032890.1,
UniProt accession no.	Q61003,

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable