Anti-CD44 Antibody (human/mouse), PE-Cy7, clone IM7 MABF583

eCl@ss:	32160702
NACRES:	NA.44

The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or “standard” form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, throµgh which it mediates adhesion.The IM7 antibody may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain. (Xu et al, 2002, J. Leukoc. Biol. 72:1133-1141). It has been reported to be cross-reactive with many non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus, Horse, Cow, Pig, Dog and Cat CD44.
This rat monoclonal antibody Anti-CD44 (human/mouse), PE-Cy7, clone IM7 validated for use in western blotting, IP, IHC & Flow Cytometry for the detection of CD44.
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD44 in one million C57Bl/6 splenocytes.

Quality Level	100
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	IM7, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
concentration	0.2 mg/mL
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG2b
conjugate	PE-Cy7
UniProt accession no.	P15379,
shipped in	wet ice

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable