Anti-CD31/PECAM-1.3 Antibody, clone MBC 78.3

eCl@ss:

32160702

Кат. номер

MABF2033

MABF2033-25UG

Platelet endothelial cell adhesion molecule (UniProt: P16284; also known as PECAM-1, EndoCAM. GPIIA, PECA1, CD31) is encoded by the PECAM1 gene (Gene ID: 5175) in human. PECAM-1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved to generate the mature protein with a large extracellular region (aa 28-601) that contains six Ig-like C2-type domains, followed by a transmembrane segment (aa 602-620) and a cytoplasmic tail (aa. 621-738 of isoform Long) that plays an essential role in PECAM-1-mediated cellular signaling. PECAM-1 is essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. It is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Six isoforms of PECAM-1 have been described that are produced by alternative splicing. Isoform Long predominates in all tissues examined, whereas Delta 12 isoform is detected only in trachea and isoform Delta14-15 are only detected in lung tissue. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia and U-937 histiocytic lymphoma cell lines. Tyrosine 690 residue plays a critical role in TEM and is required for efficient trafficking of PECAM-1 to and from the lateral border recycling compartment (LBRC) and is also required for the LBRC membrane to be targeted around migrating leukocytes. PECAM-1 also prevents phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.
Specificity
Clone MBC 78.3 detects CD31/PECAM-1 in human and canine cells. It targets an epitope within the IgG homology domain 1.
PECAM-1 purified from human platelets.
Epitope: extracellular domain
Flow Cytometry Analysis: A representative lot detected CD31/PECAM-1.3 in Flow Cytometry applications (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8).
Flow Cytometry Analysis: 1 µg from a representative lot detected CD31/PECAM-1.3 in THP-1 cells.
Western Blotting Analysis: A representative lot detected CD31/PECAM-1.3 in Western Blotting applications (Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73).
Agonist or Inhibitor Analysis: A representative performed agonist or Inhibitor function (Newton-Nash, D.K., et. al. (1999). J Immunol. 163(2):682-8; Reedguist, K.A., et. al. (2000). J Cell Biol. 148(6):1151-8).
Immunoprecipitation Analysis: A representative lot detected CD31/PECAM-1.3 in Immunoprecipitation applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27).
Immunocytochemistry Analysis: A representative lot detected CD31/PECAM-1.3 in Immunocytochemistry applications (Newman, D.K., et. al. (2016). Sci Signal. 9(418):ra27; Zhao, T., et. al. (2001). J Cell Biol. 152(1):65-73).
Anti-CD31/PECAM-1.3, clone MBC 78.3 , Cat. No. MABF2033 is a mouse monoclonal antibody that detects PECAM1 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist or Inhibitor function, and Western Blotting.
Research Category
Inflammation & Immunology
Quality
Evaluated by Immunocytochemistry in un-permeablilized THP-1 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CD31/PECAM-1.3 in un-permeablilized THP-1 cells.
Target description
82.54 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	MBC 78.3, monoclonal
species reactivity	canine, human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_000433,
UniProt accession no.	P16284,
Gene Information	human ... PECAM1(5175)

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable