Anti-CD1b Antibody, clone BCD1b3.1

eCl@ss:

32160702

Кат. номер

MABF2031

MABF2031-25UG

T-cell surface glycoprotein CD1b (UniProt: P29016; also known as CD1b) is encoded by the CD1B gene (Gene ID: 910) in human. CD1b is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. The most prominent known function of CD1b molecules is to associate with lipids from pathogens in order to stimulate T cells and induce host defense mechanisms. CD1b is expressed on cortical thymocytes, certain T-cell leukemia, and other tissues. Two isoforms of CD1b are reported that are produced by alternative splicing. CD1b has an extracellular domain (aa 18-3-3), a helical domain (aa 304-324), and a cytoplasmic tail (aa 329-332). The newly synthesized CD1b molecules follow an intracellular transport pathway that leads it to the cell surface from endoplasmic reticulum and subsequently to internalization from the plasma membrane into the endocytic pathway. After internalization, CD1b molecules are sorted for transport to lysosomes in the sorting endosome. (Ref.: Briken, V., et al. (2002). EMBO J. 21(4); 825-834).
Specificity
Clone BCD1b3.1 detects T-cell surface glycoprotein CD1b in human and guinea pig.
Isolated human CD1+ monocytes.
Anti-CD1b, clone BCD1b3.1, Cat. No. MABF2031, is a mouse monoclonal antibody that detects T-cell surface glycoprotein CD1b and it has been tested for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition assays, and Neutralizing function.
Immunohistochemistry Analysis: A representative lot detected CD1b in Immunohistochemistry applications (Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).
Flow Cytometry Analysis: A representative lot detected CD1b in Flow Cytometry applications (Hiromatsu, K., et. al. (2002). Immunology. 106(2):159-72; Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88; Sieling, P.A., et. al. (2005). J Immunol. 174(5):2637-44; Facciotti, F., et. al. (2011). Proc Natl Acad Sci USA. 108(34):14228-33).
Immunoprecipitation Analysis: A representative lot detected CD1b in Immunoprecipitation applications (Briken, V., et. al. (2002). EMBO J. 21(4):825-34; Dascher, C.C., et. al. (1999). J immunol. 163(10):5478-88).
Neutralizing Analysis: A representative lot detected CD1b in Neutralizing applications (Torrelles, J.B., et. al. (2011). J Biol Chem. 286(41):35438-46).
Inhibition Analysis: A representative lot detected CD1b in Inhibition applications (Behar, S.M., et. al. (1995). J Exp Med. 182(6):2007-18).
Research Category
Inflammation & Immunology
Quality
Evaluated by Flow Cytometry in MOLT4 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD1b in one million MOLT4 cells.
Target description
36.94 kda calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	BCD1b3.1, monoclonal
species reactivity	guinea pig, human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","inhibition assay: suitable","neutralization: suitable
isotype	IgG1
NCBI accession no.	NP_001755,
UniProt accession no.	P29016,

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable