Anti-CD166 (ALCAM) Antibody, clone TAG-1A3

eCl@ss:

32160702

Кат. номер

MABN1785

MABN1785-25UL

CD166 antigen (UniProt: Q13740; also known as Activated leukocyte cell adhesion molecule, CD166) is encoded by the ALCAM (also known as MEMD) gene (Gene ID: 214) in human. CD166 is a cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6 and homotypic cell-cell contacts. It also promotes T-cell activation and proliferation via its interactions with CD6 and is shown to be involved in the formation and maturation of the immunological synapse via its interactions with CD6. CD166 mediates attachment of dendritic cells onto endothelial cells via homotypic interaction and inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. It has an extracellular (aa 28-527), transmembrane (aa 528-549), and a cytoplasmic (aa 550-583) domain. CD166 is expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. It is also detected on hematopoietic stem cells derived from umbilical cord blood, on lymph vessel endothelial cells, skin and tonsil, and on peripheral blood monocytes. Lower levels of CD166 are detected in spleen, placenta, and liver. CD166 is also detected in highly metastasizing melanoma cell lines.
Specificity
Clone TAG-1A3 specifically detects CD166 in human.
Corneal endothelial cells (hCENC) isolated from donated human cadaves and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant.
Anti-CD166 (ALCAM) Antibody, clone TAG-1A3, Cat. No. MABN1785, is a highly specific mouse monoclonal antibody that targets CD166 and has been tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Research Category
Neuroscience
Flow Cytometry Analysis: A representative lot detected CD166 (ALCAM) in Flow Cytometry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
Immunocytochemistry Analysis: A representative lot detected CD166 (ALCAM) in Immunocytochmistry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
Immunoprecipitation Analysis: A representative lot detected CD166 (ALCAM) in Immunoprecipitation applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
Flow Cytometry Analysis: A represetative lot detected CD166 (ALCAM) in human embryonic stem cells, human corneal stromal fibroblast (hCSF) cells, immortalized human corneal endothelial cells (B4G12), and human endothelial cells from donors (2) (Courtesy of Dr. Vanessa Ding, Ph.D. and Dr. Andre Choo, Ph.D, BTI, Singapore).
Immunofluorescence Analysis: A representative lot detected CD166 (ALCAM) in Immunofluorescence applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
Western Blotting Analysis: A representative lot detected CD166 (ALCAM) in Western Blotting applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).
Quality
Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1 .
Target description
~80 kDa observed; 65.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	TAG-1A3, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunofluorescence: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_001618,
UniProt accession no.	Q13740,
shipped in	ambient
Gene Information	human ... ALCAM(214)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable