Anti-CD127 (IL-7Ra) Antibody (mouse), Biotin, clone A7R34 MABF536

eCl@ss:	32160702
NACRES:	NA.41

The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).
This rat monoclonal antibody Anti-CD127 (IL-7Ra) (mouse), Biotin, clone A7R34 validated for use in IP, Flow Cytometry & IHC for the detection of CD127 (IL-7Ra).
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 µg of this antibody detected CD127 (IL-7Ra) in one million C57Bl/6 splenocytes.

Quality Level	100
biological source	rat
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	A7R34, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µg
concentration	0.5 mg/mL
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable
isotype	IgG2a
conjugate	biotin conjugate
UniProt accession no.	Q60943,
shipped in	wet ice

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable