Anti-CD127 (IL-7Ra) Antibody (mouse), APC, clone A7R34 MABF535

eCl@ss:	32160702
NACRES:	NA.41

The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).
Immunogen information for this antibody is proprietary information.
This rat monoclonal antibody Anti-CD127 (IL-7Ra) (mouse), APC, clone A7R34 validated for use in IP, Flow Cytometry & IHC for the detection of CD127 (IL-7Ra).
Research Category
Inflammation & Immunology
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD127 (IL-7Ra) in one million C57Bl/6 splenocytes.
Physical form
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Storage and Stability
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	A7R34, monoclonal
species reactivity	mouse
packaging	antibody small pack of 25 µg
concentration	0.2 mg/mL
application(s)	flow cytometry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable
isotype	IgG2a
conjugate	Allophycocyanin conjugate
UniProt accession no.	P16872,
shipped in	wet ice

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable