Anti-CD11b Antibody (human/mouse), PE-Cy5, clone M1/70 MABF515

eCl@ss:	32160702
NACRES:	NA.44

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling throµgh ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.
Immunogen information for this antibody is proprietary information.
This rat monoclonal antibody Anti-CD11b (human/mouse), PE-Cy5, clone M1/70 validated for use in IP, IHC, Flow Cytometry & Immunofluorescence for the detection of CD11b.
Research Category
Inflammation & Immunology
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 µg of this antibody detected CD11b in one million C57Bl/6 bone marrow cells.
Physical form
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Storage and Stability
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	rat
antibody form	purified antibody
antibody product type	primary antibodies
clone	M1/70, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
concentration	0.2 mg/mL
application(s)	flow cytometry: suitable","immunofluorescence: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable
isotype	IgG2b
conjugate	PE-Cy5
UniProt accession no.	P05555,","P11215,
shipped in	wet ice

WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable