Anti-CD117 (c-Kit) Antibody, clone 2C11

eCl@ss:

32160702

Кат. номер

MABF2053-25UG

MABF2053-100UG

Mast/stem cell growth factor receptor Kit (UniProt: P10721; also known as EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) is encoded by the KIT (also known as SCFR) gene (Gene ID: 3815) in human. C-Kit is a single-pass type I membrane protein with tyrosine protein kinase activity and serves as a cell-surface receptor for the cytokine KITLG/SCF and is shown to play a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis and other functions. c-Kit exists as a monomer in the absence of bound KITLG/SCF, however, it homodimerizes in the presence of bound KITLG/SCF and forms a heterotetramer with two KITLG/SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and KITLG/SCF binding enhances its autophosphorylation. Its kinase activity is down-regulated by phosphorylation on serine residues by PKC family members. Phosphorylation at Tyrosine 568 is essential for its interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to its internalization and degradation. c-Kit is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form with an extracellular domain (aa 26-524), a short transmembrane domain (aa 525-545), and a cytoplasmic domain (aa 546-976. Its two ATP-binding sites are localized to amino acids 596-603 and 671-677. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy.
Specificity
Clone 2C11 specifically detects CD117 (c-Kit) in human and murine cells. It targets an epitope with in the extracellular domain.
GST-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of Human C-kit.
Flow Cytometry Analysis: A representative lot detected CD117 (c-Kit) in P815 cells (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).
Western Blotting Analysis: A representative lot detected CD117 (c-Kit) in DLD-1 cell lysate (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).
This mouse monoclonal Anti-CD117 (c-Kit), clone 2C11 Antibody, Cat. No. MABF2053 is tested for use in Western Blotting and Flow Cytometry, for the detection of C-kit.
Quality
Evaluated by Western Blotting in DLD-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CD117 (c-Kit) in DLD-1 cell lysate.
Target description
~160 kDa observed; 109.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Concentration: Please refer to lot specific datasheet.

biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	2C11, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_000213.1,
UniProt accession no.	P10721,
Gene Information	human ... KIT(3815)