Anti-Caspase 3, Active antibody produced in rabbit

MDL number:	MFCD01321906
NACRES:	NA.41

Кат. номер

C8487-200UL

C8487-100UL

C8487-25UL

Caspase-3 (CASP3) belongs to the class of cysteine proteases. CASP3 is an executioner caspase, which is cleaved and activated with the help of caspase-8 and caspase-9 initiator caspases. CASP3 is mapped to human chromosome 4q35.1.
Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.
Synthetic peptide corresponding to the cleavage site of human caspase 3 conjugated to KLH.
Anti-Caspase 3, Active antibody has been used:

• for western blotting of cytochrome c for caspase activation
• as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
• in Western blot analysis of activated caspase 3
• as a primary antibody in immunodetection of rat brain sections

Biochem/physiol Actions
Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.
Physical form
Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be storage, the product may be stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	canine, human, bovine, pig, rat, mouse
packaging	antibody small pack of 25 µL
application(s)	indirect immunofluorescence: 1:1,000 using human epitheloid carcinoma HeLa cell line, treated with staurosporine","microarray: suitable","western blot: 1:500 using recombinant human caspase 3, active (Sigma Product No. C1224)
conjugate	unconjugated
UniProt accession no.	P42574,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... CASP3(836) mouse ... Casp3(12367) rat ... Casp3(25402)

pictograms	GHS07
signalword	Warning
hcodes	H312 - H412
pcodes	P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable