Anti-Calnexin antibody produced in rabbit

MDL number:	MFCD01094399
NACRES:	NA.44

Кат. номер

C4731-.2ML

C4731-25UL

Calnexin contains a long (461 amino acids) N-terminal Ca2+ -binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) transmembrane segment and an acidic cytosolic domain (96 amino acids).
Calnexin, is a calcium binding type I integral membrane protein present mainly in the endoplasmic reticulum. It plays a pivotal role in quality control processes during protein synthesis and folding. Anti-calnexin antibody (diluted 1: 300) can be used in dual immunofluorescence staining to visualize TG2 transport.
Specificity
Rabbit anti-calnexin antibody reacts specifically with calnexin (90 kDa) of dog, mouse, rat and human.
Synthetic peptide corresponding to the c-terminus of human calnexin (amino acids 573-592) conjugated to KLH.
Anti-Calnexin antibody produced in rabbit has been used in:

• western blot analysis
• immunofluorescence
• dual immunofluorescence staining

Biochem/physiol Actions
Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin-like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. Calnexin has been shown to be associated with several cell surface proteins, including major histocompatibility complex (MHC) class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the endoplasmic reticulum (ER). It also forms complexes with other resident ER proteins involved in Ca2+ -dependent retention of proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen 90 kDa
species reactivity	canine, mouse, human, rat
packaging	antibody small pack of 25 µL
application(s)	immunoprecipitation (IP): 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line","indirect immunofluorescence: 1:200 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line","microarray: suitable","western blot: 1:2,000 using whole cell RIPA lysate of the human hepatocytoma HepG2 cell line
conjugate	unconjugated
UniProt accession no.	P27824,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... CANX(821) mouse ... Canx(12330) rat ... Canx(29144)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2