Anti-Calnexin Antibody, clone AF8

eCl@ss:

32160702

Кат. номер

MABF2067

MABF2067-25UG

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a well-conserved, calcium binding, endoplasmic reticulum (ER) resident chaperone protein that associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is cleaved to generate the mature form. Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at Ser564 by MAPK3/ERK1, which increases its association with ribosomes.
Specificity
Clone AF8 detects Calnexin in human cells.
TCR complexes isolated from the human T-cell leukemia line, DND41.
Anti-Calnexin, clone AF8, Cat. No. MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Flow Cytometry Analysis: 1 µg from a representative lot detected Calnexin in one million MCF7 cells.
Immunoprecipitation Analysis: A representative lot detected Calnexin in Immunoprecipitation applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8; Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).
Immunohistochemistry Analysis: A representative lot detected Calnexin in Immunohistochemistry applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).
Flow Cytometry Analysis: A representative lot detected Calnexin in Flow Cytometry applications (Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).
Western Blotting Analysis: A representative lot detected Calnexin in Western Blotting applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Calnexin in HeLa, A431 and Huvec cell lines.
Research Category
Inflammation & Immunology
Quality
Evaluated by Immunocytochemistry in MCF7 cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Calnexin in MCF7 cells.
Target description
67.57 kDa calculated.
Replaces: MAB3126
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	AF8, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µg
application(s)	flow cytometry: suitable","immunocytochemistry: suitable","immunohistochemistry: suitable","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_001019820.1,
UniProt accession no.	P27824,
Gene Information	human ... CANX(821)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable