Anti-Calcitonin Gene-Related Peptide antibody, Mouse monoclonal

MDL number:	MFCD00162203
NACRES:	NA.41

Кат. номер

C7113-100UL

C7113-25UL

CGRP (Calcitonin gene-related peptide) belongs to calcitonin family and is expressed mainly in the central and peripheral nervous systems. It is a strong endogenous vasodilator. CGRP concentration increases in migraine attacks and hence, it is involved in the pathogenesis of migraine headaches . Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunoneutralization and radioimmunoassay. Mouse anti-calcitonin gene-related peptide antibody reacts specifically with an epitope present within the C-terminal ten amino acids of rat α-CGRP and also has shown cross reactivity with α and β-CGRP of humans and dogs.
Calcitonin gene-related peptide (CGRP) is a vasodilator that belongs to the calcitonin family of peptides. It is produced by motor neurons of peripheral and central nervous system. There are two forms of CGRP, and , which exhibit similar functions. The effects of CGRP are mediated by G-protein coupled receptor heterodimer formed by calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1). Several biological effects of CGRP are observed in nervous, endocrine, immune and cardiovascular systems. Multiple studies have implicated CGRP in the pathophysiology of migraine and antagonists to CGRP are being explored as treatment options
Monoclonal Anti-Calcitonin Gene-Related Peptide (CGRP) recognizes rat -CGRP. The antibody cross-reacts with human and dog - and -CGRP.
rat α-CGRP peptide. The epitope recognized by the antibody resides within the C-terminal ten amino acids of rat α-CGRP.
Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunocytochemistry and immunohistochemistry. It can also be used in ELISA.
Anti-Calcitonin Gene-Related Peptide (CGRP) antibody is suitable for ELISA at a working concentration of 0.25- 0.5 μg/ml. The antibody may be used for immunofluorescence at a working dilution of 1:100 in rat nerve fibres and human dorsal root ganglia.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper),
Immunohistochemistry (1 paper),
Packaging
100 μL in glass insert
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	4901, monoclonal
form	buffered aqueous solution
species reactivity	human, rat, canine
packaging	antibody small pack of 25 µL
concentration	~2 mg/mL
application(s)	immunocytochemistry: suitable","indirect ELISA: 0.5 µg/mL using rat -CGRP","neutralization: suitable","radioimmunoassay: suitable
isotype	IgG1
conjugate	unconjugated
UniProt accession no.	P01258,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... CALCA(796) rat ... Calca(24241)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable