Anti-c-Jun Antibody, clone 6E4.4

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

5-1076

5-1076-25UG

5-1076-100UG

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Specificity
This antibody detects c-Jun, regardless of phosphorylation state.
GST-tagged recombinant protein corresponding to c-Jun.
Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).
Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
Research Sub Category
Transcription Factors
Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.
Target description
~36-43 kDa
Multiple bands may be visible, indicating various phosphorylation states.
Replaces: 04-210
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Storage and Stability
Stable at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	6E4.4, monoclonal
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µg
application(s)	immunocytochemistry: suitable","western blot: suitable
isotype	IgG1
NCBI accession no.	NP_002219.1,
UniProt accession no.	P45983,
shipped in	ambient