Anti-BrdU antibody, Mouse monoclonal

MDL number:	MFCD00164550
NACRES:	NA.41

Кат. номер

B8434-25UL

B8434-100UL

B8434-200UL

5-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that is selectively incorporated into cell DNA at the S phase of the cell cycle.
Anti-BrdU antibody, Mouse monoclonal (mouse IgG1) is derived from the BU-33 hybridoma produced by the fusion of murine myeloma cells and splenocytes from BALB/c mouse immunized with bromodeoxyuridine (BrdU) conjugated to KLH.5-Bromo-2-deoxyuridine (BrdU) staining is predominantly used to observe the multiplication of tumor cells and other tissues in vivo.
Specificity
Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.
bromodeoxyuridine conjugated to KLH.
Anti-BrdU antibody has been used in:

• BrdU labeling
• immunohistochemistry
• immunostaining
• BrdU assay

Anti-BrdU antibody, Mouse monoclonal has been used in immunostaining, as a primary antibody:

• to detect 5-fluorouridine-labelled RNA
• to detect 5-bromo-2-deoxyuridine (BrdU) labelled immunoreactive cells
• against BrdU labelled rat retinal sections

Packaging
200 μL in glass bottle
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified from hybridoma cell culture, purified immunoglobulin
antibody product type	primary antibodies
clone	BU-33, monoclonal
form	buffered aqueous solution
species reactivity	wide range
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 µg/mL using sections of intestine from mice or rats treated in vivo with BrdU
isotype	IgG1
conjugate	unconjugated
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable