Anti-Bin1 Antibody, clone 2F11

eCl@ss:	32160702
NACRES:	NA.41

Кат. номер

MABC1190-25UG

MABC1190

Myc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. It contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles.
Specificity
Clone 2F11 recognizes an epitope within the N-terminal BAR domain of Bin 1.
GST-tagged recombinant fragment corresponding to the BAR domain (aa 29-276) from the N-terminal half of human Myc box-dependent-interacting protein 1 (Bin1).
Epitope: domain of:
Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.
Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).
Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).
Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.
Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).
Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).
Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190, is a mouse monoclonal antibody that detects Bridging integrator 1 (Bin1) protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Detect Bin1 using this mouse monoclonal Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Apoptosis & Cancer
Quality
Evaluated by Immunohistochemistry (Paraffin) in human cerebellum and human skeletal muscle tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Bin1 in human cerebellum and human skeletal muscle tissue.
Target description
64.70 kDa calculated.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	2F11, monoclonal
species reactivity	mouse, rat, human, chicken
packaging	antibody small pack of 25 µg
application(s)	immunohistochemistry: suitable (paraffin)","western blot: suitable
isotype	IgG1","IgG1
NCBI accession no.	NP_647593.1,
UniProt accession no.	O00499,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable