Anti-Aurora B antibody produced in rabbit

MDL number:	MFCD02262515
NACRES:	NA.41

Кат. номер

A5102-25UL

A5102-200UL

Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.
Specificity
Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.
Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
Anti-Aurora B antibody has been used in:

• in immunoblotting
• in immunoprecipitation
• in immunofluorescence
• in immunofluorescence staining
• in western blotting

Biochem/physiol Actions
Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.
Target description
Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	IgG fraction of antiserum
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen 41 kDa
species reactivity	human, mouse, rat
packaging	antibody small pack of 25 µL
application(s)	immunoprecipitation (IP): suitable","indirect immunofluorescence: 1:50 using HeLa cells","microarray: suitable","western blot: 1:1,000 using nuclei-enriched fraction of mouse NIH-3T3 cells","western blot: 1:1,000 using using PC-12 rat phaeochromocytoma cells
conjugate	unconjugated
UniProt accession no.	Q96GD4,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... AURKB(9212) mouse ... Aurkb(20877) rat ... Aurkb(114592)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable