Anti-ASPP2 (TP53BP2) Antibody, clone DX54.10

eCl@ss:

32160702

Кат. номер

MABC1092-25UL

MABC1092

Apoptosis-stimulating of p53 protein 2 (UniProt: Q13625; also known as Bcl2-binding protein, Bbp, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53-binding protein 2, 53BP2, p53-binding protein 2, p53BP2) is encoded by the TP53BP2 (also known as ASPP2, BBP) gene (Gene ID: 7159) in human. 53BP2 plays a central role in regulation of apoptosis and cell growth. It is widely expressed and its expression is observed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte 53BP2 is induced following DNA damage by UV radiation. It is down-regulated by wild-type p53/TP53, but not by mutant p53/TP53. It regulates p53/TP53 action by enhancing the DNA binding and transactivation function of TP53 on the promoters of pro-apoptotic genes in vivo. 53BP2 is shown to inhibit the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. 53BP2 contains four ankyrin repeats and one SH3 domain are required for a specific interactions with TP53. The 53BP2 binding site on the p53 core domain consists of evolutionarily conserved regions that are frequently mutated in human cancers. Lower level of 53BP2 expression is observed in breast carcinomas expressing a wild-type TP53 protein.
Specificity
Clone DX54.10 detects Apotosis -stimulating of p53 protein 2 (TP53BP2) in human cells. It targets an epitope with in 438 amino acids from the C-terminal half.
GST-tagged recombinant fragment corresponding to 438 amino acids from the C-terminal half of human Apoptosis-stimulating of p53 protein 2 (TP53BP2).
Epitope: C-terminus
Immunoprecipitation Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunoprecipitation applications (Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).
Immunohistochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunohistochemistry applications (Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).
Immunocytochemistry Analysis: A representative lot detected ASPP2 (TP53BP2) in Immunocytohemistry applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Wang, Y., et. al. (2014). Nat Cell Biol. 16(11):1092-104).
Western Blotting Analysis: A representative lot detected ASPP2 (TP53BP2) in Western Blotting applications (Wang, Y., et. al. (2013). Cell Death Differ. 20(4):525-34; Samuels-Lev, Y., et. al. (2001). Mol Cell. 8(4):781-94).
Anti-ASPP2 (TP53BP2), clone DX54.10, Cat. No. MABC1092, is a mouse monoclonal antibody that detects Apoptosis-stimulating of p53 protein 2 (ASPP2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Research Category
Apoptosis & Cancer
Quality
Evaluated by Western Blotting in U2OS-iASPP and U2OS RISC-free cell lysates.
Western Blotting Analysis: A 1:125 dilution of this antibody detected ASPP2 in lysates of U2OS cells transfected with siRNA to knock down iASPP and in RISC-free U2OS cells.
Target description
~170 kDa observed; 125.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100
biological source	mouse
antibody form	purified antibody
antibody product type	primary antibodies
clone	DX54.10, monoclonal
species reactivity	human
packaging	antibody small pack of 25 µL
application(s)	immunocytochemistry: suitable","immunohistochemistry: suitable (paraffin)","immunoprecipitation (IP): suitable","western blot: suitable
isotype	IgG1
UniProt accession no.	Q13625,
shipped in	ambient

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable