Anti-ALS2CL (N-terminal region) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

SAB4200166-25UL

SAB4200166-200UL

The amyotrophic lateral sclerosis 2 (ALS2) C-terminal like (ALS2CL) gene is an ALS2 homologous gene, mapped to human chromosome 3p21.31. The encoded protein is homo-dimeric and forms an heteromeric complex with ALS2 oligomer which localize to the membrane compartments.
Specificity
Anti-ALS2CL (N-terminal region) specifically recognizes human ALS2CL.
Anti-ALS2CL (N-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
The co-expression of amyotrophic lateral sclerosis 2 (ALS2) C-terminal like (ALS2CL) along with Ras-related protein Rab-5A (Rab5) results in the distinctive tubulation phenotype of endosome compartments. It is responsible for the co-localization of ALS2CL and Rab5. ALS2CL protein has weak Rab5-guanine nucleotide exchange factor (GEF) activity, but predominantly adheres to Rab5. Also, it inhibits the ALS2 mediated endosome enlargement. ALS2CL might be considered as a critical moiety over ALS2, in modulating the ALS2 and Rab5 mediated endosome dynamics in the cell. The N-terminal region of the ALS2CL protein specifically controls endolysosomal trafficking induced by endosome-autophagosome. It is involved in the regulation of neurite growth.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at 20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~125 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1.5 mg/mL
application(s)	western blot: 1.5-3.0 µg/mL using SH-SY5Y cell lysates.
conjugate	unconjugated
UniProt accession no.	Q60I27,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... ALS2CL(259173)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable