Anti-ADAR1 Antibody, clone RD4B11

eCl@ss:

32160702

Кат. номер

MABE1790-25UG

MABE1790-100UG

Double-stranded RNA-specific adenosine deaminase (UniProt: Q99MU3; also known as EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1) is encoded by the Adar gene (Gene ID: 56417) in murine species. ADAR catalyzes the editing of adenosine-to-inosine (A to I) by deaminating genomically encoded A-to-I in double stranded RNA (DsRNA). A-to-I editing predominantly occurs in noncoding, repetitive elements and short interspersed nuclear elements (SINEs). A-to-I editing affects gene expression and function in several ways, including mRNA translation by changing codons and hence the amino acid sequence of proteins. It also affects pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; and genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication. In mammals three different ADAR proteins have been described: ADAR1, -2, and -3. ADAR1 is shown to be expressed during embryonic and postnatal development and is present predominantly in the nucleus. Highest levels of ADAR1 are reported in brain and spleen and lowest levels are observed in liver. Five different isoforms of ADAR1 have been reported that are produced by alternative splicing. Isoforms 1 and 2 (long forms) are predominantly found in cytoplasm and shuttle between the cytoplasm and nucleus. Isoforms 3 and 4 (short forms starting at Met-519) are exclusively found in the nucleolus. Under normal conditions long forms are dominant, however, under conditions of inflammation short forms are selectively induced. (Ref.: Liddicoat, BJ et al., (2015). Science 349 (6252); 1115-1120).
Specificity
Clone RD4B11 is a rat monoclonal antibody that detects Double-stranded RNA-specific adenosine deaminase in human and murine cells.
His-tagged recombinant fragment corresponding to 401 amino acids from the internal region of murine Double-stranded RNA-specific adenosine deaminase (ADAR1). Immunogen sequnece is conserved isoforms 1 and 2.
Western Blotting Analysis: A representative lot detected ADAR1 in Western Blotting applications (Liddicoat, B.J., et. al. (2015). Science. 349(6252):1115-20).
Anti-ADAR1, clone RD4B11, Cat. No. MABE1790, is a highly specific rat monoclonal antibody that targets ADAR1 and has been tested for use in Western Blotting.
Quality
Evaluated by Western Blotting in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.
Western Blotting Analysis: 4 µg/mL of this antibody detected ADAR1 in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.
Target description
~110 kDa observed; 130.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Concentration: Please refer to lot specific datasheet.

biological source	rat
antibody form	purified immunoglobulin
antibody product type	primary antibodies
clone	RD4B11, monoclonal
species reactivity	mouse, human
packaging	antibody small pack of 25 µg
application(s)	western blot: suitable
isotype	IgG2a
NCBI accession no.	NP_062629.3,
UniProt accession no.	Q99MU3,