Anti- Actinin antibody, Mouse monoclonal

NACRES:

NA.41

Кат. номер

SAB4200813-25UL

SAB4200813-100UL

Monoclonal Anti- α Actinin (mouse IgM isotype) is derived from the hybridoma BM-72.5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells. α-Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle, α-actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle, α-actinin has been detected in dense bodies and plaques characteristic of the tissue.
Specificity
Monoclonal Anti-α-Actinin specifically recognizes α-Actinin from human1, mouse2, rat3, chicken4, monkey, canine and bovine origin.
Cytoskeletal fraction of bovine mammary gland epithelium (BMGE) cultured cells.
Anti-α Actinin antibody may be used in various immunochemical techniques including Immunoblot (~100 kDa), Immunohistochemistry and Immunofluorescence. Monoclonal Anti-α-Actinin may be used for immunofluorescent localization of α-actinin in cultured cells and tissues, for immunofluorescent labeling of skeletal muscle (normal and pathological) in order to detect its organizational state, studies of membrane anchorage sites and immunochemical identification of α-actinin by immunoblotting analysis.
Biochem/physiol Actions
α-actinin has extensive proteins association with actin containing stress fibers, in particular with their membrane bound termini.
α-actinin modulates muscle contraction and has a role in cytoskeletal organisation. α-actinin acts as an actin crosslinker and scaffold. Mutations in this gene has been linked to heterogeneous hypertrophic cardiomyopathy and juvenile onset atrial fibrillation.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	BM-75.2, monoclonal
mol wt	~100 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
concentration	~1 mg/mL
application(s)	immunoblotting: 0.5-1 µg/mL using human HeLa cells extract.","immunofluorescence: 5-10 µg/mL using human foreskin fibroblast Hs68 cells.
isotype	IgM
UniProt accession no.	P12814,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... ACTN1(87)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable