- Производитель:
- Sigma-Aldrich
MDL number: | MFCD00145889 |
NACRES: | NA.41 |
Кат. номер |
A2103-200UL |
A2103-25UL |
Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.
Anti-Actin, N-terminal antibody produced in rabbit has been used in
- immunoblotting
- quantitative chromatin immunoprecipitation (qChIP)
Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle.
Biochem/physiol Actions
Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | rabbit |
Quality Level | 200 |
antibody form | affinity isolated antibody |
antibody product type | primary antibodies |
clone | polyclonal |
form | buffered aqueous solution |
mol wt | antigen 42 kDa |
species reactivity | mouse, frog, rat, human, chicken |
packaging | antibody small pack of 25 µL |
concentration | ~0.5 mg/mL |
application(s) | immunocytochemistry: 1-2 µg/mL using cultured chicken fibroblasts","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 µg/mL using sections of human appendix, mouse heart, and frog skeletal muscle","indirect immunofluorescence: suitable","western blot: 0.5-1 µg/mL using whole extract of the human epitheloid carcinoma HeLa cell line.","western blot: 2-4 ng/mL using whole extract of rat skeletal muscle |
conjugate | unconjugated |
UniProt accession no. | P63261, |
shipped in | wet ice |
storage temp. | 20°C |
Gene Information | human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72) mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468) rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365) |
Personal Protective Equipment | Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US), |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
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