Anti-2A Peptide

Кат. номер

ABS31-I-25UG

ABS31-I-100UG

Genome polyprotein (UniProt: P03305) is encoded by the D1Y31_gp1 gene (Gene ID: 37627025) in Foot-and-mouth disease virus. Following its synthesis, it is cleaved into 15 different chains. P2A chain resides in the amino acids 936-953 in the internal region. 2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 16-20 amino acids (depending on the virus of origin). They contain a highly conserved sequence, GDVEXNPGP, at the C-terminus, which is shared by different 2As and is essential for the creation of steric hindrance and ribosome skipping. These peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multi-cistronic construct. These peptides are used by several families of viruses, the best-known amongst these are foot-and-mouth disease virus 2A (F2A) and Thosea asigna virus 2A (T2A). This rabbit polyclonal antibody reacts with protein in lysate from HEK293T cells transfected with various 2A-tagged proteins. (Ref.: Liu, Z., et al. (2017). Scientific Reports.7; Article number: 2193).
Specificity
This rabbit polyclonal antibody detects 2A peptide tagged proteins.
KLH-conjugated linear peptide corresponding to 18 amino acids (936-953) 2A peptide of Foot-and-mouth disease virus.
Quality Control Testing
Evaluated by Western Blotting in lysate from HEK293T cells transfected with CD3/g/d/e chains.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 2A in lysate from HEK293T cells transfected with CD3/g/d/e chains.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound 2A peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-2A Peptide, Cat. No. ABS31-I, is a rabbit polyclonal antibody that detects 2A peptide tagged proteins and is tested for use in Affinity Binding Assay and Western Blotting.
Physical form
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	purified antibody
antibody product type	primary antibodies
clone	polyclonal
mol wt	calculated mol wt 258.93 kDa","observed mol wt ~21, 26, and 31 kDa
purified by	affinity chromatography
species reactivity	all
packaging	antibody small pack of 100 µg
application(s)	affinity binding assay: suitable","western blot: suitable
isotype	IgG
epitope sequence	Internal
conjugate	unconjugated
UniProt accession no.	P03305,
shipped in	2-8°C
Gene Information	Unknown ... D1Y31_gp1(37627025)