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CelLytic™ Y Cell Lysis Reagent
NACRES: NA.56
Кат. номер C4482-500ML-PW C4482-50ML-PW C4482-1.5ML C4482-50ML C4482-250ML C4482-500ML C4482-10X1.5ML General descriptionCelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.ApplicationCelLytic™ Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).
Features and Benefits- Fast: Cell lysis in just 20 minutes
- Efficient: Three times more efficient than glass beads
- Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
- Gentle: Non-denaturing - preserves biological activity
Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200,
Safety InformationRIDADR NONH for all modes of transport Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lysozyme chloride form from chicken egg white
CAS Number: 9066-59-5 Enzyme Commission (EC) Number: 3.2.1.17 ( BRENDA | IUBMB ) EC Number: 232-954-0 MDL number: MFCD00131557 NACRES: NA.54
PackagingКат. номер L2879-5G L2879-25G L2879-1G
1, 5, 25 g in poly bottleApplicationEnzyme which breaks down the cell walls of bacteria; used to prepare spheroplasts.
Unit Definition
One unit will produce a A450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Physical form
Lyophilized powder containing sodium chloride and sodium acetate
Preparation Note
3x CrystallizedПараметрыQuality Level 200, type Grade VI form lyophilized powder specific activity 35,000 units/mg protein (E1%/282) mol wt ~14.3 kDa composition Protein, 80% storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lysozyme from human neutrophils
CAS Number: 9001-63-2 Enzyme Commission (EC) Number: 3.2.1.17 ( BRENDA | IUBMB ) MDL number: MFCD00131557 NACRES: NA.54
Кат. номер L8402-.1MG-PW L8402-.1MG General descriptionLysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.ApplicationLysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.
Biochem/physiol Actions
Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.
Unit Definition
One unit will produce a A450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Physical form
Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaClПараметрыQuality Level 200 assay 95% (SDS-PAGE) form lyophilized powder specific activity 100,000 units/mg protein (E1%/280) UniProt accession no. P61626, storage temp. 20°C Gene Information human ... LYZ(4069)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lysozyme human
CAS Number: 12671-19-1 Enzyme Commission (EC) Number: 3.2.1.17 ( BRENDA | IUBMB ) MDL number: MFCD00131557 NACRES: NA.54
Кат. номер L1667-10G L1667-1G L1667-100MG L1667-10MG General descriptionLysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.ApplicationLysozyme human has been used:- in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
- in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
- in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)
Packaging
1, 10 g in glass bottle
Package size based on protein content
Biochem/physiol Actions
Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.
Features and Benefits
Lysobac™ is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac™ has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:- Improves Bacterial Fermentation Efficiency
- Greater bacterial lysis activity per mg.
- 1 gram of delivers more lysis activity than 4 grams of HEWL
- Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
- Improved recombinant protein yield compared to mechanical lysis
- Reduces cost of goods
Unit Definition
One unit will produce a A450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).Legal InformationLysobac is a trademark of InVitriaПараметрыQuality Level 200 recombinant expressed in rice assay 90% (SDS-PAGE) form lyophilized powder specific activity 100,000 units/mg protein mol wt 16.5 kDa UniProt accession no. P61626, shipped in dry ice storage temp. 70°C Gene Information human ... LYZ(4069)
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lyticase from Arthrobacter luteus
CAS Number: 37340-57-1 MDL number: MFCD00131560 NACRES: NA.54
Кат. номер L2524-10KU-PW L2524-200KU-PW L2524-25KU-PW L2524-50KU L2524-10KU L2524-200KU L2524-25KU L2524-50KU-PW Packaging10000, 25000 units in glass bottle
50000, 200000 units in poly bottleBiochem/physiol ActionsLyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.Unit DefinitionOne unit will produce a A800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.Physical formPartially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizersПараметрыQuality Level 300 form lyophilized powder specific activity 2,000 units/mg protein composition Protein, 20% biuret Featured Industry Diagnostic Assay Manufacturing storage temp. 20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H334 pcodes P261 - P284 - P304 + P340 + P312 - P501 Personal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Lyticase from Bacillus subtilis
CAS Number: 144941-36-6 NACRES: NA.54
Unit DefinitionКат. номер 4963-5G 4963-25G 4963-1G
1U corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 C (Yeast, Cat. No. 51475, as substrate).Параметрыform liquid, viscous liquid Quality Level 100, specific activity 500 U/mL solubility H2O: 1:10, clear, colorless to very faintly green suitability corresponds for SDS-PAGE storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mutanolysin Assay Substrate
Кат. номер M3440-PH M3440-1VL-PW M3440-1VL M3440-PM ApplicationThe activity of mutanolysin can be determined by hydrolysis of the rehydrated cell suspension in a continuous spectrophotometric assay.ПараметрыQuality Level 200, form (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.) packaging vial of 10 assays shipped in wet ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields,, Gloves,, type N95 (US), RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mutanolysin from Streptomyces globisporus ATCC 21553
CAS Number: 55466-22-3 MDL number: MFCD00131635 NACRES: NA.54
Кат. номер M9901-5KU-PW M9901-10KU-PW M9901-1KU-PW M9901-10KU M9901-1KU M9901-50KU M9901-5KU M9901-50KU-PW General descriptionMutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.ApplicationMutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.Packaging1000, 5000, 10000, 50000 units in glass bottleBiochem/physiol ActionsMutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the -N-acetylmuramyl-(14)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.Unit DefinitionOne unit will produce a A600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.Physical formLyophilized powder containing Ficoll® and sodium succinate buffer saltsLegal InformationFiberfrax is a registered trademark of Unifrax Corp.ПараметрыQuality Level 300 form lyophilized powder specific activity 4000 units/mg protein (biuret) mol wt 23 kDa Featured Industry Diagnostic Assay Manufacturing shipped in wet ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves, type N95 (US) RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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