Sigma-Aldrich
- Сортировать:
- Вид таблицей
-
Pur-A-Lyzer™ Mega Dialysis Kit PURG35010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 10 mL displacement packaging pkg of 10 Mega dialysis tubes capacity 3-20 mL pore size 3.5 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG12020-1KT
General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 20 mL displacement packaging pkg of 10 Mega dialysis tubes capacity 3-20 mL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG12015-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 15 mL displacement packaging pkg of 10 Mega dialysis tubes capacity 3-20 mL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG12010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 10 mL displacement packaging pkg of 10 Mega dialysis tubes capacity 3-20 mL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG60020-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 20 mL displacement packaging pkg of 10 Mega dialysis tubes capacity 3-20 mL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG10015-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 15 mL displacement packaging pkg of 5 Mega dialysis tubes (in individual pouch) capacity 3-20 mL pore size 1 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG10010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 10 mL displacement packaging pkg of 5 Mega dialysis tubes (in individual pouch) capacity 3-20 mL pore size 1 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mega Dialysis Kit PURG10020-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыfeature cap 20 mL displacement packaging pkg of 5 Mega dialysis tubes (in individual pouch) capacity 3-20 mL pore size 1 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD10005-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
Pur-A-Lyzer dialysis tube kits are easy-to-use devices in a screw-cap tube format and are disposable. They contain a dual dialysis membrane window for buffer exchange. Samples are added and removed using a standard laboratory pipette and come in 4 different versions to accommodate a range of sample volumes.- High sample recovery >97%
- Protease-, RNase-, and DNase-free
- Convenient sample loading- no syringes necessary
- Versatile- dialyze proteins, RNA, and DNA
Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 5 Maxi dialysis tubes capacity 50-800 µL pore size 1 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD35010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 10 Midi dialysis tubes capacity 50-800 µL pore size 3.5 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD35050-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 50 Midi dialysis tubes capacity 50-800 µL pore size 3.5 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD35100-1KT
General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 100 Midi dialysis tubes capacity 50-800 µL pore size 3.5 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD60010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 10 Midi dialysis tubes capacity 50-800 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD60030-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 30 Midi dialysis tubes capacity 50-800 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD60100-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 100 Midi dialysis tubes capacity 50-800 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD60050-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 50 Midi dialysis tubes capacity 50-800 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Midi Dialysis Kit PURD35030-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.
The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.Application- Dialysis or buffer exchange of volumes between 10 mL and 20 mL
- Preparation of protein samples for MALDI-MS
- Sample concentration
- Large-scale protein dialysis such as antibodies and recombinant protein purification
- Peptide dialysis for molecules as small as 10 amino acids
- Virus-particle purification
- Removal of contaminating micro molecules
- Purification of tissue culture extracts
- Removal of salts, surfactants, solvents and detergents
- Complex formation studies (protein-protein, protein-DNA and protein-RNA)
- pH and buffer adjustment of sample solution, protein extract or cell extract
- High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 µL
Midi: 50-800 µL
Maxi: 0.1-3 mL
Mega: 3-20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription includes floating rack and handbook packaging pkg of 30 Midi dialysis tubes capacity 50-800 µL pore size 3.5 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN25005-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 5 Mini dialysis tubes (in individual pouch) capacity 10-250 µL pore size 25 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN12100-1KT
General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 100 Mini dialysis tubes capacity 10-250 µL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN12050-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 50 Mini dialysis tubes capacity 10-250 µL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN12030-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 30 Mini dialysis tubes capacity 10-250 µL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN12010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 Mini dialysis tubes capacity 10-250 µL pore size 12-14 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN60010-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 10 Mini dialysis tubes capacity 10-250 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN60100-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 100 Mini dialysis tubes capacity 10-250 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN60050-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 50 Mini dialysis tubes capacity 10-250 µL pore size 6-8 kDa MWCO Узнать цену -
Pur-A-Lyzer™ Mini Dialysis Kit PURN60030-1KT
NACRES: NB.22 General descriptionThe Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield. The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.
The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.ApplicationDialysis or buffer exchange of volumes between 10 mL and 20 mL
Preparation of protein samples for MALDI-MS.
Sample concentration.
Large-scale protein dialysis such as antibodies and recombinant protein purification.
Peptide dialysis for molecules as small as 10 amino acids.
Virus-particle purification.
Removal of contaminating micro molecules.
Purification of tissue culture extracts.
Removal of salts, surfactants, solvents and detergents.
Complex formation studies (protein-protein, protein-DNA and protein-RNA)
pH and buffer adjustment of sample solution, protein extract or cell extract.
High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad.
For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10 - 250 µL
Midi: 50 - 800 µL
Maxi:0.1 - 3 mL
Mega: 3 - 20 mLLegal InformationPur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLCПараметрыpackaging pkg of 30 Mini dialysis tubes capacity 10-250 µL pore size 6-8 kDa MWCO Узнать цену -
Chloroplast Isolation Kit
NACRES: NA.32
Кат. номер CPISO-1KT CPISO-1KT-PW General descriptionThe Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.ApplicationChloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.Features and Benefits- Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
- Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
- Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
- Convenient format - Saves time and reduces waste
SuitabilityThe Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.ПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Endoplasmic Reticulum Isolation Kit
Кат. номер ER0100-1KT ER0100-1KT-PW General descriptionThe endoplasmic reticulum (ER) is a network of interconnected tubules, vesicles, and sacs. It plays a role in specialized cellular functions including protein synthesis, sequestering of calcium, production of steroids, storage and production of glycogen, and insertion of membrane proteins.ApplicationThe Endoplasmic Reticulum Isolation Kit contains all the reagents required for ER preparations with various degrees of purity: crude ER (microsomes), Ca2+ precipitated rough ER (RER) enriched microsomes, and density gradient purified rough ER (RER) and smooth ER (SER). The ER isolation process can be monitored by measuring the activity of NADPH cytochrome c reductase using the Cytochrome c Reductase Assay Kit, (Cat. No. CY0100). The enzyme is an ER membrane protein that is commonly used as an ER marker. The isolated ER can be used for studying the cytochrome P450 system and xenobiotic metabolism, for studying lipid metabolism, and for the recovery of ER membrane and lumenal proteins. The kit was tested with rat liver, kidney, and brain; mouse liver; rabbit liver, kidney, spleen, heart, and brain; and with Jurkat and HeLa cell lines.
The Endoplasmic Reticulum Isolation Kit is used for the efficient enrichment of functional endoplasmic reticulum (ER) from mammalian tissue or cell culture. The isolated ER are useful for protein synthesis, degradation and metabolic pathway analysis. Metabolic abnormalities of ER include carnitine acetyltransferase deficiency in children, cystic fibrosis and many more ER protein retention disorders. Suitable for up to 50 g of tissue or ~20 mL of packed tissue.Features and Benefits- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Includes a calcium chloride solution - for quick and simple precipitation of rough ER without need for ultracentrifugation
- Produces functional, intact organelles - resulting ER are suitable for functional studies, lipid metabolism, and protein profiling
- Compatible with products for structure confirmation - easily confirm intactness with companion kit test for cytochrome C reductase (CY0100)
- Can be used to isolate ER from soft animal tissues and cultured cells
ПараметрыQuality Level 200 storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Golgi Isolation Kit
NACRES: NA.32
Кат. номер GL0010-1KT GL0010-1KT-PW General descriptionThe Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).ApplicationGolgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.Analysis NoteThe Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.ПараметрыQuality Level 200 usage sufficient for 50 g (tissue) shipped in wet ice storage temp. 2-8°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
Lysosome Isolation Kit
EC Number: 233-140-8 NACRES: NA.32
Кат. номер LYSISO1-1KT-PW LYSISO1-1KT General descriptionLysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.ApplicationLysosome Isolation Kit has been used:- in the isolation of cytosol and lysosome
- to obtain enriched light and heavy lysosomal fractions from cultured cells
- in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
- in lysosome purification
The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.Features and Benefits- Generates functional organelles for metabolism and disease research
- Includes protease inhibitor to manage degradation
- Measures integrity with included Neutral Red dye
- Choose from multiple options to obtain enriched fractions
- Enriched fractions for intact and functional lysosomes from tissues and cells
- Suitable for functional studies including protein degradation
ПараметрыQuality Level 200 usage sufficient for 20 mL (packed cells), sufficient for 25 g (tissue) storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H319 pcodes P264 - P280 - P305 + P351 + P338 - P337 + P313 RIDADR UN 1993 / PGIII Flash Point F 188.6 °F Flash Point C 87 °C Узнать цену -
Mammalian Cell Lysis Kit
NACRES: NA.56
Кат. номер MCL1-1KT MCL1-1KT-PW ApplicationThe product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.Features and Benefits- The kit can be used to prepare cell lysis buffer and other modified buffers
- The kit enables the researcher to change the buffers components to obtain maximum efficiency for a specific protein
ComponentsThe mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.ПараметрыQuality Level 200 usage 1 kit sufficient for 250 extractions (100 mm tissue culture plates) shipped in dry ice storage temp. −20°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H302 - H315 - H318 - H412 pcodes P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 RIDADR NA 1993 / PGIII WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Mitochondria Isolation Kit
NACRES: NA.32
Кат. номер MITOISO2-1KT MITOISO2-1KT-PW ApplicationMitochondria Isolation Kit has been used in the isolation of mitochondria from Ewing′s Sarcoma Family of Tumor (ESFT) cells, blood samples from tuberculosis patients, human neuroblastoma cells and glioma cells, myotubes and HepG2 cells.
The Mitochondria Isolation Kit provides a fast and easy method for the isolation of an enriched mitochondrial fraction from cells. Most of the isolated mitochondria will contain inner and outer membranes. The kit contains extraction buffers for both functional studies and proteome profiling. Also included is a storage buffer for intact mitochondria.ПараметрыQuality Level 200 usage sufficient for 50 applications (2-5 x 107 cells) shipped in dry ice storage temp. 20°C
Safety Informationpictograms GHS07,GHS08,GHS09 signalword Warning hcodes H302 - H351 - H361fd - H411 pcodes P202 - P264 - P270 - P273 - P301 + P312 - P308 + P313 RIDADR UN 3316 9 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
Peroxisome Isolation Kit
NACRES: NA.32
Кат. номер PEROX1-1KT PEROX1-1KT-PW General descriptionIsolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.ApplicationThe Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.Features and Benefits- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
- Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)
Other NotesUpon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.Legal InformationOptiPrep is a trademark of Alere Technologies ASПараметрыQuality Level 200 shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 pcodes P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 RIDADR UN 1993 / PGIII WGK Germany WGK 2 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
ProteoPrep® Universal Extraction Kit PROTTWO-1KT
NACRES: NA.32 ApplicationThis kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.- Fraction 1: Soluble/Cytoplasmic Proteins
- Fraction 2: Membrane Proteins
Features and Benefits- Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
- Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
- Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
- Pre-weighed dry blends - Stable and easy to reconstitute
- Conveniently Packaged - No waste. Use only the amount needed.
Legal InformationProteoPrep is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 form dry powder storage temp. 2-8°C
Safety Informationpictograms GHS02,GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H251 - H301 - H314 - H317 - H335 - H351 - H360FD - H411 pcodes P235 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 Target Organs Respiratory system RIDADR UN 3316 9 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Yeast Mitochondria Isolation Kit
NACRES: NA.32
Кат. номер MITOISO3-1KT MITOISO3-1KT-PW General descriptionMitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.ApplicationThe kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria. Yeast Mitochondria Isolation Kit has been used in the isolation and extraction of mitochondria.Features and Benefits• The kits features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombeПараметрыQuality Level 200 usage sufficient for 40 applications (using 20 OD culture preparations) storage temp. −20°C
Safety Informationpictograms GHS05,GHS06,GHS08,GHS09 signalword Danger hcodes H301 + H331 - H312 - H315 - H318 - H334 - H351 - H361fd - H411 pcodes P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311 - P305 + P351 + P338 RIDADR UN 3316 9 Узнать цену -
Benzonase® Nuclease, ultrapure
CAS Number: 9025-65-4 Enzyme Commission (EC) Number: 3.1.30.2 ( BRENDA | IUBMB ) MDL number: MFCD00131010 NACRES: NA.54
Кат. номер E8263-25KU E8263-5KU-PW E8263-25KU-PW E8263-5KU ApplicationThe enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.
Ultrapure benzonase nuclease, or Endonuclease from Serratia marcescens, has been used in a study to assess the effect of sodium dodecylbenzenesulfonate and antifoaming agents on the endonuclease activity of Serratia marcescens. Endonuclease from Serratia marcescens has also been used in a study to investigate the structure of chromatin subunits.
Used for the removal of nucleic acid from protein samples.Biochem/physiol ActionsBenzonase® is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.
Digests native or heat-denatured DNA and RNA.Unit DefinitionOne unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a A260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml).Physical formSolution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.Legal InformationBenzonase® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, GermanyПараметрыQuality Level 200 recombinant expressed in E. coli grade ultrapure grade assay 99% (SDS-PAGE) form buffered aqueous glycerol solution mol wt 30 kDa concentration 250 units/µL shipped in wet ice storage temp. 20°C
Safety InformationPersonal Protective Equipment Eyeshields, Gloves RIDADR NONH for all modes of transport WGK Germany WGK 1 Узнать цену -
CelLytic™ B Cell Lysis Reagent
MDL number: MFCD02097909
Кат. номер B7435-500ML B7435-50ML-KC B7435-500ML-KC B7435-50ML ApplicationCelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human -synuclein.
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.Features and Benefits- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other NotesDetergent blend formulated in 40 mM Trizma® HCl (pH 8.0).Legal InformationCovered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200
Safety Informationpictograms GHS07 signalword Warning hcodes H315 - H319 pcodes P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 Personal Protective Equipment Eyeshields, Gloves, type ABEK (EN14387) respirator filter RIDADR NONH for all modes of transport WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ B Plus Kit CB0050-1KT
EC Number: 200-664-3 NACRES: NA.56 General descriptionThe CelLytic™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.ApplicationThis kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chromatography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.
Features and Benefits- Lyse Gram positive and Gram negative bacteria
- More efficient than sonication
- Compatible with affinity purification
- Isolate inclusion bodies for subsequent solubilization
- Non-denaturing cell lysis preserves protein function
Other NotesThe CelLytic™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.Legal InformationBenzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, usage 1 kit sufficient for 5 g fresh or frozen cell paste lysis. shipped in wet ice storage temp. 20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H315 - H319 - H334 pcodes P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338 RIDADR NA 1993 / PGIII Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ B Plus Kit CB0500-1KT
EC Number: 200-664-3 NACRES: NA.56 General descriptionThe CelLytic™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.ApplicationThis kit is compatible with FLAG®, HIS-Select®, and glutathione S-transferase based affinity chromatography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.
Features and Benefits- Lyse Gram positive and Gram negative bacteria
- More efficient than sonication
- Compatible with affinity purification
- Isolate inclusion bodies for subsequent solubilization
- Non-denaturing cell lysis preserves protein function
Other NotesThe CelLytic™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.Legal InformationBenzonase® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, usage 1 kit sufficient for 50 g fresh or frozen cell paste lysis. shipped in wet ice storage temp. 20°C
Safety Informationpictograms GHS08 signalword Danger hcodes H315 - H319 - H334 pcodes P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338 RIDADR NA 1993 / PGIII Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ Express
NACRES: NA.56
Кат. номер C1990-10X25ML C1990-25ML C1990-6X500ML C1990-500ML C1990-500ML-KC General descriptionCelLytic™ Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic™ Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic™ Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.ApplicationCelLytic™ Express has been used as a component in lysis buffer for protein purification.
CelLytic Express is optimized for the lysis of E. coli strain BL21. However, it works well with other commons strains such as JM109. CelLytic Express may also be used on other similar bacterial cells. CelLytic Express has been tested with BL21 E. coli cells expressing histidine-tagged and FLAG fusion proteins. It should be compatib with affinity purification of other fusion proteins.
CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.
Packaging
25, 10×25 mL in poly bottle
Features and Benefits- Efficient and non-denaturing in-culture protein extraction
- Save time by eliminating cell harvest and clarification steps
- Preserve biological activity with fewer manipulations
- Compatible with affinity resins and magnetic beads
- Ideal for Production Scale Extraction protocols
Preparation Note
CelLytic® Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic™ Express, and not by adding them to the dry powder.
Storage and Stability
This product ships on wet ice and storage at –20°C is recommended. CelLytic™ Express, as supplied, is stable for at least two years when stored properly.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLC
Celatom is a registered trademark of EP Minerals, LLCПараметрыshipped in wet ice Quality Level 200, storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 3 Узнать цену -
Cellytic™ Express, 1 mL Tablets
Кат. номер C5491-25EA C5491-100EA
Packaging
25, 100 ea in poly bottleApplicationAll the advantages of CelLytic™ Express now in a tablet format. For direct lysis of bacterial cultures and for use in the His-Select® iLAP® column. CelLytic Express tablets are a complete formulation including lysozyme and endonuclease for complete cell lysis. The resulting lysate is clear of cellular debris, and is immediately ready for standard affinity purification. This new formulation adds minimal volume to the final lysate, providing a method with defined volume and time parameters, making it easier to validate and making it more convenient for production scale protocols.
Features and Benefits- Efficient and non-denaturing in-culture protein extraction
- Save time by eliminating cell harvest and clarification steps
- Preserve biological activity with fewer manipulations
- Compatible with affinity resins and magnetic beads
- Ideal for Production Scale Extraction protocols
Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC
iLAP is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200, usage 1 tablet sufficient for 1 mL lysis (cell suspension) storage temp. 20°C
Safety InformationRIDADR NONH for all modes of transport Узнать цену -
CelLytic™ IB Inclusion Body Solubilization Reagent
PackagingКат. номер C5236-25ML C5236-100ML
25 mL in poly bottleApplicationCelLytic™ IB was designed to solubilize protein aggregates called inclusion bodies, which are formed in bacteria when recombinant proteins are overexpressed. CelLytic™ IB was formulated to solubilize the protein of interest for immediate analysis of protein content or refolding procedures.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыstorage temp. room temp Quality Level 200,
Safety Informationpictograms GHS07 signalword Warning hcodes H302 - H315 - H319 pcodes P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ M
NACRES: NA.56
Кат. номер C2978-120ML C2978-10ML-PW C2978-120ML-PW C2978-250ML-PW C2978-60ML C2978-10ML C2978-250ML C2978-40ML C2978-50ML C2978-50ML-PW General descriptionCelLytic M is a proprietary detergent solution designed for efficient whole-cell protein extraction from cultured mammalian cells. It enables efficient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications without removing the CelLytic M such as reporter gene assays, Western blots/immunoprecipitation, electrophoretic mobility shift assays, phosphatase assays and kinase asssays.Features and Benefits- Efficient: Up to 50% more efficient than freeze thaw, sonication and other products
- Non-denaturing: Does not interfere in downstream applications such immunoprecipitation, kinase and phosphatase assays, reporter gene assays and gel shift assays
- Convenient: Ready-to-use reagent requires no scraping from culture plates
- Fast: Rapid cell lysis at room temperature.
QuantityCelLytic M reagent efficiency for protein extraction has been tested on, but not limited to, HeLa, CHO, COS, HL-60, Jurkat, A431, PC-12, and Bovine Aorta Endothelial Cells (BAEC).Analysis NoteUse 125 µl CelLytic M for 106-107 of suspended cells. For adherent cells, use 500-1,000 µL for a 100 mm plate; 200-400 µL for a 35 mm plate.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 storage temp. room temp
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ MEM Protein Extraction Kit
NACRES: NA.56
Кат. номер CE0050-1KT CE0050-1KT-PW General descriptionCelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
Suitability
Sufficient reagents supplied for 80 tests.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 shipped in wet ice storage temp. 20°C
Safety InformationFlash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
CelLytic™ MT Cell Lysis Reagent
NACRES: NA.56
Кат. номер C3228-500ML-PW C3228-50ML C3228-500ML C3228-50ML-PW ApplicationCelLytic™ MT Cell Lysis Reagent has been used in the homogenization of hippocampi tissues, quadriceps muscles from mice, primary human trophoblasts (PHT), soleus muscles from mice and organs of Corti (OCs).Features and Benefits- Gentle: Non-denaturing and does not interfere with downstream applications
- Convenient: Provided ready to use
Other NotesCelLytic MT is an efficient reagent for the extraction of proteins. The lysis buffer consists of a dialyzable mild detergent, bicine, and 150 mM NaCl, resulting in minimal interference with protein interactions and biological activity. CelLytic MT is also used for extraction of cell-line proteins. A volume of 20 mL of CelLytic MT is sufficient for 1 gram of tissue.QualityIt has been tested, but not limited to the following tissues: rat brain, kidney, muscle, heart, liver, spleen and mouse brain, kidney and muscle.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200
Safety InformationRIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ NuCLEAR™ Extraction Kit
NACRES: NA.56
Кат. номер NXTRACT-1KT NXTRACT-1KT-PW General descriptionThe procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.ApplicationNXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.Other NotesWithin this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. CelLytic NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.LinkageRecommended Antibodies for Immunodetection L1293, N2662, AMAB90549Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLC
NuCLEAR is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 300 usage 1 kit sufficient for 10 extractions (1 ml packed cell volume), 1 kit sufficient for 100 extractions (100 µl packed cell volume) shipped in dry ice storage temp. 20°C
Safety Informationpictograms GHS05,GHS09 signalword Danger hcodes H290 - H314 - H410 pcodes P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391 RIDADR UN 3316 9 Flash Point F 188.6 °F - closed cup Flash Point C 87 °C - closed cup Узнать цену -
CelLytic™ P Cell Lysis Reagent
NACRES: NA.56
Кат. номер C2360-250ML-PW C2360-50ML C2360-250ML C2360-50ML-PW ApplicationCelLytic™ P Cell Lysis Reagent has been used for lysate preparation for cell lysis.Other NotesCelLytic™ P is a non-ionic detergent-based reagent, which offers a convenient method for efficient plant cell lysis and protein solubilization. It is a non-denaturing reagent and maintains protein immunoreactivity and biological activity. CelLytic™ P is efficient, rapid, and ready to use. It contains bicine buffer, which is preferable for many biological activities.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыdescription For plant cell lysis Quality Level 200, storage temp. room temp
Safety Informationpictograms GHS07 signalword Warning hcodes H319 - H412 pcodes P273 - P305 + P351 + P338 Personal Protective Equipment Eyeshields,, Faceshields,, Gloves,, type ABEK (EN14387) respirator filter, RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
CelLytic™ PN Isolation/Extraction Kit
NACRES: NA.56
Кат. номер CELLYTPN1-1KT CELLYTPN1-1KT-PW General descriptionCelLytic™-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.ApplicationThe nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.Other NotesThis kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.Legal InformationCelLytic is a trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 200 usage 1 kit sufficient for 30 extractions (20 g of fresh or frozen leaves) shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS05,GHS09 signalword Danger hcodes H315 - H318 - H410 pcodes P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 RIDADR UN 3077 9 / PGIII Узнать цену
Получите коммерческое предложение в течение 1 часа
Менеджер подготовит коммерческое предложение и позвонит, если понадобится уточнить детали вашего заказа
Анна Гловацкая
Менеджер по работе с клиентами
С 2010 года мы поставляем оборудование с заводов Европы. Берем на себя все — от подбора оборудования до внедрения на предприятии
Все сотрудники имеют высшее образование, закончили ведущие химические вузы страны, такие как РХТУ им Менделеева.
У большинства компаний срок ожидания составляет 10-12 недель.
Оборудование хранится на сухом отапливаемом складе, где поддерживается ровная температура.
Работаем с PonyExpress и Деловыми линиями. Вы также можете выбрать свою транспортную компанию или забрать товар со склада в Москве.
В случае любых неполадок за свой счет выполним ремонт в сервисном центре или на заводе-изготовителе. Или бесплатно заменим прибор на новый.
Производим пуско-наладку оборудования, валидацию, обучение сотрудников. Если нужно, привлекаем инженеров с заводов- изготовителей.
