StableCell™ MEM M0446-500ML
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagles Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks or Earles salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Minimum Essential Medium Eagle has been used in murine fibroblast culture for
in vitro cytocompatibility testing.
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
StableCell is a trademark of Sigma-Aldrich Co. LLC
Quality Level | 400, |
sterility | sterile-filtered |
form | liquid |
application(s) | cell culture | mammalian: suitable |
impurities | endotoxin, tested |
components | phenol red: yes Earle’s salts (5% CO2): no NaHCO3: yes Hanks’ salts (2% CO2): no sodium pyruvate: no glucose: no HEPES: no L-glutamine: no stable glutamine: yes |
shipped in | ambient |
storage temp. | 2-8°C |
Personal Protective Equipment | Eyeshields,, Gloves, |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point F | Not applicable |
Flash Point C | Not applicable |