Secondary Antibodies
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Rabbit Anti-Sheep IgG Antibody, HRP conjugate 12-342
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.ApplicationRabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Quality
Routinely evaluated by western blot
Physical form
0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate
Storage and Stability
Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.Legal InformationUPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100, antibody form affinity purified immunoglobulin antibody product type secondary antibodies clone polyclonal species reactivity sheep Торговая марка Upstate® application(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable conjugate peroxidase conjugate shipped in wet ice Узнать цену -
Rabbit Anti-Sheep IgG Antibody, HRP conjugate AP147P
eCl@ss: 32160702 NACRES: NA.46 General descriptionAffinity purified rabbit anti-sheep IgG, Peroxidase conjugated
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.ApplicationEIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
This Rabbit anti-Sheep IgG Antibody, HRP conjugate is validated for use in ELISA, WB for the detection of Sheep IgG.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Physical form
ImmunoAffinity Purified
RECONSTITUTION:
Reconstitute with 1.5 mL of sterile distilled water.
Storage and Stability
STORAGE: Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100, antibody form affinity purified immunoglobulin antibody product type secondary antibodies clone polyclonal species reactivity sheep Торговая марка Chemicon® application(s) ELISA: suitable, western blot: suitable conjugate peroxidase conjugate shipped in wet ice
Safety InformationWGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Rabbit IgG HRP Linked Whole Ab GENA934-1ML
General descriptionThe antibodies are supplied with dilution guidelines for relevant applications and should be stored at 2° C to 8° C.Features and Benefits- Highly species-specific.
- Optimized for use with our range of ECL™ Detection Reagents.
- Recommended dilutions to minimize background.
Legal InformationECL is a trademark of CytivaПараметрыantibody form F(ab)2 fragment of affinity isolated antibody antibody product type secondary antibodies shelf life Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service. feature wetted part: no packaging pkg of 1 mL Торговая марка Cytiva NA934-1ML color Brown (light) conjugate peroxidase conjugate shipped in wet ice storage temp. 2-8°C Узнать цену -
Rabbit-Anti-Human IgG4 Antibody, clone RM120 4-1667
eCl@ss: 32160702 NACRES: NA.46
Specificity
This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.ImmunogenPeptide corresponding to the hinge region of Human IgG4.ApplicationImmunohistochemistry Analysis (IHC): 1 -10 µg/mL from a representative lot detected human IgG4 in human lymphoid tissue.
ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Rabbit-Anti-Human IgG4 Antibody, clone RM120 is an antibody against Human IgG4 for use in Western Blotting, Immunohistochemistry, ELISA.
Quality
Evaluated by Western Blotting in human, mouse, rat, and goat IgG.
Western Blotting Analysis (WB): 2 µg/mL of this antibody detected human IgG in human, mouse, rat, and goat IgG.
Target description
(~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and
mouse, rat, or goat IgG.
Physical form
Format: Purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Protein A purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source rabbit Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone RM120, monoclonal species reactivity human application(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable isotype IgG shipped in wet ice Узнать цену -
Sheep anti-Human IgG Antibody AC112
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
This antibody demonstrates minimal to no cross reactivity against Mouse IgG, Rabbit IgG, Rat IgG, Hamster IgG, Guinea Pig IgG, and Chicken IgY.
This antibody demonstrates specificity with Human IgG (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc).ImmunogenPurified human IgGApplicationResearch Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Sheep anti-Human IgG Antibody detects level of Sheep Human IgG & has been published & validated for use in Sheep Human IgG.
ELISA Analysis: As little as 0.025 µg/mL of this antibody detected Human IgG (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc).
Quality
Evaluated by ELISA in a panel of antibodies containing Mouse IgG, Rabbit IgG, Rat IgG, Hamster IgG, Guinea Pig IgG, and Human IgG.
ELISA Analysis: As little as 0.025 µg/mL of this antibody detected Human IgG with minimal to no cross-reactivty against Mouse IgG, Rabbit IgG, Rat IgG, Hamster IgG and Guinea Pig IgG.
Target description
~150 kDa observed.
Physical form
Octanoic Acid and Ammonium Sulfate cut then depleted
Purified sheep polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
Format: Semi-Purified
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity human concentration 1 mg/mL application(s) ELISA: suitable shipped in wet ice
Safety InformationWGK Germany WGK 1 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Human IgG Antibody, Cy5 conjugate AC112S
eCl@ss: 32160702 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins, which stage immune responses against pathogenic organisms. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc gamma receptors which interact with sialylated Fc fragments on IgG molecules. The coupling of Cy5 to Anti-Human IgG antibody allows for the visualization of proteins by fluorescent microscopy.
Specificity
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC112), this antibody exhibits strongest immunoreactivity towards whole human IgG molecule (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc) with minimal or no cross-reactivity toward IgG from chicken, guinea pig, hamster, mouse, rabbit, and rat species.ImmunogenPurified human IgG.ApplicationResearch Category
Secondary & Control Antibodies
Research Sub Category
All
This Sheep Anti-Human IgG, Cy5 conjugate, Cat. No. AC112S, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.
This antibody is also available in unconjugated and Cy3-conjugated forms (Cat. Nos. AC112 and AC112C).
Quality
Evaluated by Immunocytochemistry in human B-cell hybridoma SA13.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody immunostained 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human B-cell hybridoma SA13, but not HeLa cells.
Target description
~150 kDa observed.
Physical form
Ammonium sulfate.
Purified sheep polyclonal antibody Cy5 conjugate in PBS with 1.5% BSA and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity human application(s) immunocytochemistry: suitable conjugate CY5 conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Human IgM, (Fc) Antibody AP512
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobin M (IgM) antibody is prominent in early immune responses to most antigens and is the predominant response in certain antibody responses such as "natural" blood group antibodies. IgM is the major immunoglobin expressed on surface B lymphocytes. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt′s lymphoma bear surface IgM, whereas plasmocytes from Waldenström′s disease bear intracytoplasmic IgM.
Specificity
This antibody reacts with human IgM, (Fc) as demonstrated by ELISA.
No reactivity with IgG or IgA. May react with IgM of other species with low specificity.ImmunogenEpitope: IgM, (Fc)
Human anti-Human IgM (myeloma) Fc5u fragmentApplicationResearch Category
Secondary & Control Antibodies
Sheep anti-Human IgM, (Fc) Antibody is an antibody against Human IgM, (Fc) for use in ELISA.
Research Sub Category
Isotype Control Antibodies
Quality
Evaluated by ELISA against Human IgG, Human IgG Fab Fragment, Human IgG Fc, Human IgM Fc, Human IgM (myeloma) Fab m fragment, Human IgA, Mouse IgM, Rat IgM whole molecule, Chicken IgM whole molecule, Goat IgM whole molecule, Guinea Pig IgM whole molecule, Hamster IgM whole molecule, and Rabbit IgM whole molecule.
ELISA Analysis: A representative lot detected Sheep Anti-Human IgM, (Fc) in ELISA.
Target description
50 kDa calculated
Physical form
Depletion
Format: Purified
Purified sheep polyclonal in PBS containing 0.05% sodium azide. Depleted against rabbit IgM and human IgG.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human IgG, Human IgG Fab Fragment, Human IgG Fc, Human IgM Fc, Human IgM (myeloma) Fab m fragment, Human IgA, Mouse IgM, Rat IgM whole molecule, Chicken IgM whole molecule, Goat IgM whole molecule, Guinea Pig IgM whole molecule, Hamster IgM whole molecule, and Rabbit IgM whole moleculeLegal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity human Торговая марка Chemicon® application(s) ELISA: suitable shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate AC111P
eCl@ss: 32160702 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.ImmunogenPurified mouse IgG.ApplicationResearch Category
Secondary & Control Antibodies
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.
Research Sub Category
All
Western Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate.
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate.
Target description
~150.0 kDa observed.LinkageReplaces: AP300P
Physical form
Ammonium Sulfate.
Purified sheep polyclonal antibody in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Proclin.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationStabilZyme is a registered trademark of SurModics, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity mouse application(s) western blot: suitable conjugate peroxidase conjugate shipped in ambient
Safety Informationpictograms GHS07 signalword Warning hcodes H317 - H412 pcodes P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate AC111B
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.ImmunogenPurified mouse IgG.ApplicationResearch Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate is an antibody against Sheep Mouse IgG for use in Immunohistochemistry.
Immunohistochemistry Analysis: A representative lot was validated by immunohistochemistry staining (1:500 dilution) of human liver tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Quality
Evaluated by Immunohistochemistry in human prostate tissue.
Immunohistochemistry Analysis: Performance of this biotin-conjugated secondary antibody has been validated by immunohistochemistry staining (1:500 dilution) of human prostate tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Target description
~150 kDa observed.
Physical form
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to biotin conjugation.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity mouse application(s) immunohistochemistry: suitable isotype IgG1 conjugate biotin conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy3 conjugate AC111C
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with mouse IgG whole molecule (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity to chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.ImmunogenPurified mouse IgG.ApplicationResearch Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy3 conjugate is an antibody against Mouse IgG for use in Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot was validated by indirect fluorescent labelling of trimethylated histone H3 in HeLa and NIH/3T3 cells following a mouse monoclonal primary antibody (Cat. No. 05-1951) in immunocytochemistry application.
Quality
Evaluated by Immunocytochemistry in A431.
Immunocytochemistry Analysis: Performance of this Cy3-conjugated secondary antibody is validated by indirect fluorescent labelling of trimethylated histone H3 in A431 following a mouse monoclonal primary antibody (Cat. No. 05-1951) in immunocytochemistry application.
Target description
~150 kDa observed.
Physical form
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Fractionated by octanoic acid and ammonium sulfate cut and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity mouse application(s) immunocytochemistry: suitable conjugate CY3 conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate AC111S
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.ImmunogenPurified mouse IgG.ApplicationResearch Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate is an antibody against Sheep Mouse IgG for use in Immunocytochemistry.
Quality
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: Performance of this Cy5-conjugated secondary antibody has been validated by indirect immunofluorescent labelling (1:250 dilution) of A431 cells following a mouse monoclonal anti-H3K27me3 primary antibody (Cat. No. 05-1951).
Target description
~150 kDa observed.
Physical form
Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity mouse application(s) immunocytochemistry: suitable conjugate CY5 conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Mouse IgG Antibody, Species absorbed: Rat IgG AC111
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
This antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with Human IgG, Rabbit IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG, but may cross-react with immunoglobulins from other species.
Based on ELISA analysis, this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reaction mouse IgA or IgM.ImmunogenPurified antibody corresponding to mouse IgG.ApplicationELISA (Isotype) Analysis: A representative lot of this antibody detected whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reaction mouse IgA or IgM.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed: Rat IgG is an antibody against Sheep Mouse IgG for use in ELISA.
Quality
Evaluated by ELISA using IgG antibodies from various species.
ELISA Analysis: This antibody detected 200 ng of mouse IgG antibody among a panel of IgG antibodies from various species.
Polysorp® is a registered trademark of Thermo Fisher Scientific.
Target description
150 kDa calculated
Physical form
Format: Purified
Fractionated by Octanoic Acid and Ammonium Sulfate cut and then depleted with Rat IgG column and Mouse IgA column.
Purified sheep Polyclonal serum in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to the Certificate of Analysis for the lot-specific concentration.Legal InformationPolySorp is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity mouse application(s) ELISA: suitable shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Rabbit IgG Antibody, Cy3 conjugate AP510C
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
This antibody reacts with rabbit IgG and rabbit IgG (Fc). Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.ImmunogenRabbit IgG.ApplicationThis Sheep Anti-Rabbit IgG Antibody, Cy3 conjugate is validated for use in Immunocytochemistry for the detection of Sheep Rabbit IgG.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in HUVECs following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.
Quality
Evaluated by Immunocytochemistry in NIH/3T3 cells.
Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in NIH/3T3 following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.
Physical form
Purified sheep antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Ammonium Sulfate
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.Other NotesConcentration: Please refer to lot specific datasheet.Legal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity rabbit Торговая марка Chemicon® application(s) immunocytochemistry: suitable conjugate CY3 conjugate shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep Anti-Rabbit IgG Antibody, HRP conjugate AP510P
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules. This antibody is processed from Cat. No. AP510, Sheep anti-Rabbit IgG.
Specificity
This antibody reacts with rabbit IgG and rabbit IgG (Fc). There may be minimal cross-reactivity against Human IgG and Rabbit IgM, with minimal to no cross-reactivity against Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.ApplicationSheep Anti-Rabbit IgG Antibody, HRP conjugate validated for use in western blotting.
Quality
Evaluated by Western Blot in Rabbit IgG recombinant protein.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Rabbit IgG in 0.5 µg of Rabbit IgG recombinant protein.
Target description
~53 kDa observed
Physical form
Purified sheep polyclonal in buffer containing PBS, StabilZyme® HRP Conjugate Stabilizer, and 0.05% ProClin 150.
Analysis Note
Control
Rabbit IgG recombinant proteinLegal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
StabilZyme is a registered trademark of SurModics, Inc.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity rabbit Торговая марка Chemicon® concentration 1 mg/mL application(s) western blot: suitable conjugate peroxidase conjugate shipped in wet ice
Safety Informationpictograms GHS08 signalword Danger hcodes H360D - H412 pcodes P202 - P273 - P280 - P308 + P313 - P405 - P501 WGK Germany WGK 3 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
Sheep-Anti-Rabbit IgG secondary Antibody AP510
eCl@ss: 32160702 NACRES: NA.46 General descriptionImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
This antibody reacts with rabbit IgG and rabbit IgG (Fc) as demonstrated by ELISA. Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.ImmunogenRabbit IgGApplicationResearch Category
Secondary & Control Antibodies
Research Sub Category
Isotype Control Antibodies
Sheep-anti-Rabbit IgG secondary antibody detects level of Rabbit IgG secondary & has been published & validated for use in ELISA.
Quality
Evaluated by ELISA against Human IgG, Mouse IgG, Rat IgG, Chicken IgM, Goat IgG, Guinea Pig IgG, Hamster IgG, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM.
ELISA Analysis: A representative lot detected Sheep Anti-Human IgM, (Fc) in ELISA. (1:200 - 1,000 recommended dilution.)
Target description
150 kDa calculated
Physical form
Purified sheep polyclonal in buffer containing PBS with 0.05% sodium azide.
Format: Purified
Octanoic Acid and Ammonium Sulfate cut. Depleted against Goat IgG and Rabbit IgM
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human IgG, Mouse IgG, Rat IgG whole molecule, Chicken IgM whole molecule, Goat IgG whole molecule, Guinea Pig IgG, Hamster IgG whole molecule, Rabbit IgG, Rabbit IgG (Fc), and Rabbit IgM whole moleculeLegal InformationCHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Параметрыbiological source sheep Quality Level 100, antibody form purified antibody antibody product type secondary antibodies clone polyclonal species reactivity rabbit Торговая марка Chemicon® concentration 2 mg/mL application(s) ELISA: suitable shipped in wet ice
Safety InformationWGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену
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