Monoclonal Anti-Rabbit IgG, NativePeroxidase antibody produced in mouse

Кат. номер

R3155-200UL

R3155-25UL

Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.
Specificity
Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
Enriched fraction of rabbit IgG light chain.
Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• western blotting

Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	secondary antibodies
clone	RabT-50, monoclonal
form	buffered aqueous solution
species reactivity	rabbit
should not react with	human, pig, rat, goat, monkey, bovine, horse, chicken, cat
concentration	2.5-5.0 mg/mL (antibody)
application(s)	immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
isotype	IgG1
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable