Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse

MDL number:

MFCD00162492

Кат. номер

I9388-.5ML

I9388-.2ML

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Monoclonal Anti-Human IgG1 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG1 and does not detect IgG2, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 1x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study.
Specificity
Monoclonal Anti-Human IgG1 (Fc specific) is specific for the IgG1 subclass and nonreactive with IgG2, IgG3, and IgG4 in an ELISA. The antibody recognizes an epitope expressed in the Fc region of IgG1. The estimated association constant of this antibody with its ligand is 1x107 L/M.
Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads
Monoclonal Anti-Human IgG1 (Fc specific) antibody is suitable for use in ELISA and immunoblot.
Biochem/physiol Actions
IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for immune response against Leishmania tropica infection. It has high binding affinity towards the Fc receptors (FcR).
Physical form
Monoclonal Anti-Human IgG1 (Fc specific) is provided as ascites fluid with 15 mM sodium azide.
Storage and Stability
Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	ascites fluid
antibody product type	secondary antibodies
clone	HP-6001, monoclonal
contains	15 mM sodium azide
application(s)	indirect ELISA: 1:3,000
isotype	IgG2b
conjugate	unconjugated
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable