Monoclonal Anti-Human IgG (Fc specific)FITC antibody produced in mouse

NACRES:

NA.46

Кат. номер

F5016-.2ML

F5016-.5ML

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. The light comprises of one variable N-terminal region and a constant C-terminal region. The heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.
Specificity
The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.
Monoclonal Anti-Human IgG (Fc specific)-FITC antibody produced in mouse has been used in:

• Fluorescent Dot Immunobinding Assay (F-DIBA)
• Particle Immunofluorescent Assay (F-IFMA)
• flow cytometry

Biochem/physiol Actions
Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), containing the H chain constant region. IgG antibody have enormous therapeutic potential and the Fc is for the development of therapeutic antibody. Although the antibody site is located in the terminal end of the human IgG molecule (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibodies directed to Fc), passage through placental membrane and staphylococcus protein A binding.
Physical form
Solution in 0.01 M phosphate buffer pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	secondary antibodies
clone	HP-6017, monoclonal
form	buffered aqueous solution
species reactivity	sheep, horse (IgG), rabbit, goat, human
storage condition	protect from light
application(s)	dot immunobinding: 1:16, particle immunofluorescence: 1:16
isotype	IgG2a
conjugate	FITC conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable