Monoclonal Anti-Human IgEAlkaline Phosphatase antibody produced in mouse

NACRES:

NA.46

Кат. номер

A3076-.5ML

A3076-.2ML

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Binds human IgE; does not bind human IgG, IgA or IgM.
Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :

• immunoblot assays
• direct ELISA
• dot blot

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper),
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.
Biochem/physiol Actions
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
Analysis Note
The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	secondary antibodies
clone	GE-1, monoclonal
form	buffered aqueous solution
species reactivity	human
application(s)	direct ELISA: 1:6,000, dot blot: 1:1,000
isotype	IgG2b
conjugate	alkaline phosphatase conjugate
shipped in	wet ice
storage temp.	2-8°C

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable