Monoclonal Anti-Goat/Sheep IgGFITC antibody produced in mouse

NACRES:

NA.46

Кат. номер

F4891-.2ML

F4891-.5ML

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.
Specificity
Monoclonal Anti-Goat IgG, FITC Conjugate is immunospecific for goat IgG. The antibody does not react with human, mouse, rat, rabbit, guinea pig, horse, or chicken IgGs.
Biofile 39 Navya load
Biochem/physiol Actions
IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization. Goat antibodies against many analytes are widely used in research as primary antibodies in various assay techniques. Second antibodies are that used to detect goat antibodies may lack specificity for goat immunoglobulins, and in many instances will also recognize other immunoglobulins that appear in the preparation being tested. This results in the need for extensive adsorption of the second antibody. Therefore, using a monoclonal antibody to goat IgG that is devoid of a binding capacity to other species can serve as a useful tool in many applications.
Physical form
Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide
Storage and Stability
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
antibody form	purified immunoglobulin
antibody product type	secondary antibodies
clone	GT-34, monoclonal
form	buffered aqueous solution
species reactivity	bovine, sheep, goat
should not react with	rat, chicken, mouse, horse, feline, pig, canine, guinea pig, rabbit, human
storage condition	protect from light
application(s)	dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32
isotype	IgG1
conjugate	FITC conjugate
shipped in	dry ice
storage temp.	−20°C

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable