Goat Anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate AP200A

eCl@ss:	32160702
NACRES:	NA.46

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Specificity
Minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins.
Prepared from purified mouse IgG light chain.
Epitope: Kappa light chain
Research Sub Category
Fragment Specific Secondary Antibodies
Research Category
Secondary & Control Antibodies
Suggested dilutions:
Western Blotting: 1:5,000 -1:50,000 of the 1 mg/mL stock.
ELISA: 1:5,000 -1:50,000 of the 1 mg/mL stock.
Optimal working dilutions must be determined by the end user.
Goat anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate detects level of Mouse light chain & has been published & validated for use in ELISA & WB.
Target description
25 kDa
Physical form
Lyophilized from a solution in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.Purified by immunoaffinity chromatography.
ImmunoAffinity Purified
Storage and Stability
Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.4 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	100,
biological source	goat
antibody form	affinity purified immunoglobulin
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	mouse
Торговая марка	Chemicon®
application(s)	ELISA: suitable, western blot: suitable
isotype	IgG
conjugate	alkaline phosphatase conjugate
shipped in	wet ice

pictograms	GHS07
signalword	Warning
hcodes	H312 - H412
pcodes	P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501
WGK Germany	WGK 3