- Производитель:
- Sigma-Aldrich
eCl@ss: | 32160702 |
Specificity
Based in immunoelectrophresis, this antibody reacts with the heavy and light chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by Elisa and/ or solid phase absorbed to ensure minimal cross reactivity with chicken, guinea pig, Syrian Hamster, horse, human, mouse, rabbit, and rat serum proteins. Antibodies may cross react with immunoglobulins from other species.
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Donkey anti-Sheep IgG Antibody, Alkaline Phosphatase conjugate, Species Adsorbed detects level of Donkey Sheep IgG & has been published & validated for use in ELISA & WB.
1:5,000 - 1:50,000 for Western Blot and Elisa
Optimal working dilutions must be determined by end user.
Physical form
ImmunoAffinity Purified
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING:
For research use only; not for use as a diagnostic.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source | donkey |
Quality Level | 100, |
antibody form | affinity purified immunoglobulin |
antibody product type | secondary antibodies |
clone | polyclonal |
species reactivity | sheep |
Торговая марка | Chemicon® |
concentration | 0.6 mg/mL |
application(s) | ELISA: suitable, western blot: suitable |
conjugate | alkaline phosphatase conjugate |
shipped in | wet ice |
hcodes | H412 |
pcodes | P273 - P501 |
WGK Germany | WGK 3 |
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