Donkey Anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed AP182B

eCl@ss:	32160702
NACRES:	NA.46

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificity
Rabbit IgG
EIA: 1:50,000-1:100,000 Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000. Immunohistochemistry: 1:500-1:5,000 Flow cytometry: 1:200-1:1,000 Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000 Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Donkey anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed detects level of Donkey Rabbit IgG & has been published & validated for use in ELISA & WB.
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.15 M NaCl, pH 7.1 with 15 mg/mL BSA, and 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with 500 µL of sterile distilled water.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	donkey
Quality Level	100,
antibody form	affinity purified immunoglobulin
antibody product type	secondary antibodies
clone	polyclonal
species reactivity	rabbit
Торговая марка	Chemicon®
application(s)	ELISA: suitable, western blot: suitable
conjugate	biotin conjugate
shipped in	wet ice

hcodes	H412
pcodes	P273 - P501
WGK Germany	WGK 3