Anti-Rat IgG (whole molecule)Peroxidase antibody produced in rabbit A5795-1ML

MDL number:	MFCD00162813
NACRES:	NA.46

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG.
purified rat IgG
HA-tagged proteins over expressed in S. cerevisiae were detected by western blot using HRP-conjugated rabbit anti-rat IgG as the secondary antibody.
Anti-Rat IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.
Anti-Rat IgG (whole molecule)-Peroxidase antibody has been used as a secondary antibody in immunohistochemical applications at a dilution of 1:1500. The product has also been used for western blot analysis at 1:20,000. Furthermore, the antibody can be used for direct ELISA at 1:50,000.
Biochem/physiol Actions
Immunoglobulin (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG participates in hypersensitivity type II and type III.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Preparation Note
Adsorbed with human IgG to reduce background staining with human samples.
Prepared by two-step glutaraldehyde method.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
antibody form	affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	rat
should not react with	human
application(s)	direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750, western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract
conjugate	peroxidase conjugate
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P280 - P302 + P352
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable