Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, CF™ 488A antibody produced in donkey

NACRES:

NA.46

Кат. номер

SAB4600033-50UL

SAB4600033-250UL

Immunoglobulin G (IgG) belongs to immunoglobulin family and consists of gamma () heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named 1, 2, 3, and 4, respectively. IgG is produced and released from CD5+B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
CF™488A is a green fluorescent dye with excitation argon laser line at 488nm. CF™ 488A is minimally charged, thus, it reduces non-specific binding of antibody conjugates. CF™ 488A is a highly water-soluble and pH-insensitive dye with molecular weight of ~710. This dye has absorption/emission maxima at 490/515nm and it is highly photostable.
Specificity
Binds all guinea pig IgGs (minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)
guinea pig IgG (H+L)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper),
Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, CF™ 488A antibody produced in donkey has been used in immunostaining.
Features and Benefits
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Physical form
Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.
Preparation Note
Protect from light.
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyers own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	donkey
Quality Level	100,
antibody form	affinity isolated antibody
antibody product type	secondary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	guinea pig
concentration	~2 mg/mL
application(s)	flow cytometry: 1-10 µg/mL, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect immunofluorescence: 1-10 µg/mL
fluorescence	ex 490 nm; em 515 nm
conjugate	CF™ 488A conjugate
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable