Roche

Plasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA).

Plasminogen is used for the determination of the activity of plasminogen activators. The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test and a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA.
Features and Benefits
Contents
Lyophilizate, stabilized with bovine serum albumin.
Quality
Contaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acid
Sequence
Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen.
Preparation Note
Stabilizers: stabilized with BSA
Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl.
Storage conditions (working solution): -15 to -25 °C
A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C.
Note:
Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.)
mol wt	90-94 kDa
packaging	pkg of 20 U
Торговая марка	Roche
shipped in	wet ice

Inhibits serine proteases such as chymotrypsin, trypsin, and thrombin, and the cysteine protease papain (reversible by DTT treatment). Does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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PMSF

Empirical Formula (Hill Notation):	C₇H₇FO₂S
CAS Number:	329-98-6
Molecular Weight:	174.19
Beilstein/REAXYS Number:	2088311
MDL number:	MFCD00007424
PubChem Substance ID:	329823637

PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.
PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.

Biochem/physiol Actions

PMSF (phenylmethylsulfonyl fluoride) functions as a non-specific inhibitor of multiple proteases and other enzymes, such as palmityl coenzyme A deacylase, arylsulfatase A, acetylcholinesterase, trypsin and chymotrypsin. It is an irreversible inhibitor, and sulfonylates hydroxyl groups of the serine residues present in the active site of enzymes.

Quality

Purity: Chromatographically homogeneous

Preparation Note

Activator: PK requires Mg2+ (or Mn2+, CO2+) and K+ (or NH4+, Rb+) for full activity.
Working concentration: 17 to 170 µg/ml (0.1 to 1 mM)
Working solution: Due to the short half-life of PMSF, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may have a half-life of only 35 minutes. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.
Storage conditions (working solution): 15 to 25 °C
Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

Reconstitution

Soluble to > 10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.
Due to a short half-life, fresh solution must be added at every purification step. Under certain conditions, such as alkaline pH values, PMSF may only have a half-life of 35 min. High-salt conditions (3 M), often used to precipitate proteins, may also reverse the inhibition of PMSF. Pefabloc SC is recommended as a non-toxic, water-soluble, and water-stable alternative.

Quality Level	100
assay	98% (TLC)
mol wt	174.2
packaging	pkg of 10 g (10837091001), pkg of 25 g (11359061001)
Торговая марка	Roche
concentration	17-170 μg/mL
application(s)	western blot: suitable
pKa A	7.0
mp	92-94 °C
solubility	>10 mg/mL (Soluble to >10 mg/ml in isopropanol, ethanol, methanol, or 1,2-propanediol. Unstable in aqueous solution. In 100% isopropanol, stable for at least 9 months at +25°C.)
shipped in	ambient
storage temp.	20-25°C
SMILES string	FS(=O)(=O)Cc1ccccc1
InChI	1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
InChI key	YBYRMVIVWMBXKQ-UHFFFAOYSA-N

UN 2923 6.1(8) / PGII

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Poly(A) 10108626001

Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.

Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.
Biochem/physiol Actions
Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.
Quality
Typical analysis:
2.3µmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length 2.100 to 10.000 nucleotides
Unit Definition
1 A260 unit corresponds to 40 µg ssRNA.
Physical form
Lyophilizate, potassium salt
Preparation Note
Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
mol wt	700-3500 kDa
packaging	pkg of 100 mg
Торговая марка	Roche
absorbance ratio	A_290/260 nm 0.03-0.05, A_280/260 nm 0.28-0.32, A_250/260 nm 0.86-0.90
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Poly(A) x (dT)15 10108677001

Poly (A) x (dT)15 is used as a template of reverse transcriptases and, for example, can be applied to test the activity of reverse transriptases.
Contents: Lyophilized with NaCl, base ratio 1:1.15.

Poly(A) x (dT)15 is used as a primer for Reverse Transcriptase.
Quality
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length approx. 1,000 bp
Unit Definition
1 A260 unit equals 50 µg

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
mol wt	660 kDa
packaging	pkg of 5 U
Торговая марка	Roche
absorbance ratio	A_290/260 nm 0.13-0.17, A_280/260 nm 0.48-0.52, A_250/260 nm 0.78-0.82
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Poly(dA) 10223581001

Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.

Poly(dA) is suitable for use in:

DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
in Pyrococcus abyssi DNA polymerase assay

Sequence
Chain Length Mean chain length lot 20-17: 250-500 bases.
Unit Definition
1 A260 unit corresponds to 40 µg ssDNA.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
mol wt	330 Da
packaging	pkg of 5 U
Торговая марка	Roche
absorbance ratio	A_290/260 nm 0.03-0.12, A_280/260 nm 0.28-0.39, A_250/260 nm 0.84-0.94
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
Features and Benefits
Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Quality
Typical analysis:
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length approx. 20,000 bp
In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
Unit Definition
1 A260 unit corresponds to 50 µg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 µg ds DNA.
Preparation Note
Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

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Poly[d(I-C)]

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])
Торговая марка	Roche
mp	54 °C (NaCl, 0.1 M, pH 7.0)
absorbance ratio	A_290/260 nm 0.24-0.32, A_280/260 nm 0.61-0.67, A_250/260 nm 1.18-1.30
shipped in	dry ice
storage temp.	20°C

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Pronase

3.4.24.4 ( BRENDA | IUBMB )

Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids.
Specificity
Pronase has a broad specificity, breaking down virtually all proteins into their individual amino acids; resolves carboxylic acids and alcohols.

Use Pronase to completely hydrolyze proteins in research applications. Pronase is used for the degradation of proteins during the isolation of DNA and RNA, such as in the extraction of phage DNA or the isolation of plasmid DNA. It is not necessary to let pronase self-digest prior to use. It is also used in histochemistry and cell culture for tissue dissociation in conjunction with collagenase and trypsin, and for the production of glycopeptides from purified glycoproteins. It has been used for the dechorionation of Zebrafish embryos.
Unit Definition
A unit of non-specific protease activity increases the rate of release of folin-positive amino acids and peptides from casein, at +40 °C and pH 7.5. For one unit (U) 1 μmol/min tyrosine is released, and for the unit "PU", the value is 1 μg/minute (1 U = 181 PU); for the unit "PUK", it is 0.1/minute (measured is the change in absorbance of molybdenum blue, formed by reaction with the folin ′s reagent under conditions such that 1 PUK = 50 PU).
Preparation Note
Stabilizers: Protective effect of calcium ions
The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C
Reconstitution
The lyophilizate is soluble in water (10 to 20 mg/ml).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	non-sterile
form	lyophilized powder
specific activity	~7.0 units/mg protein (at 40°C with casein as the substrate, pH 7.5, equivalent to approximately 1270 PU/mg or approximately 25 PUK/mg.)
packaging	pkg of 1 g (10165921001), pkg of 5 g (11459643001)
Торговая марка	Roche
parameter	35-40 °C optimum reaction temp.
optimum pH	7.0-8.0
pH range	6.0 - 9.0
shipped in	wet ice
storage temp.	2-8°C

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Protease Inhibitors Set 11206893001

Contents:

Antipain dihydrochloride
Bestatin
Chymostatin
E-64
Leupeptin
Pepstatin
Phosphoramidon
Pefabloc SC
EDTA, disodium salt
Aprotinin

Specificity
Specificity: Antipain: papain, trypsin, cathepsin A and B
Bestatin: amino peptidases
Chymostatin: -, -, -, -chymotrypsin
E-64: cysteine proteases
Leupeptin: serine and cysteine proteases, such as plasmin, trypsin, papain, and cathepsin B; Pepstatin: aspartate proteases
Phosphoramidon: metallo-endopeptidases, specifically thermolysine
Pefabloc SC: serine proteases
EDTA: metalloproteases
Aprotinin: serine proteases

Protease inhibitors set has been used in immunoprecipitation. This product was also used for protein analysis using SDS-PAGE method.
Packaging
1 set of 10 individual protease inhibitors.
Preparation Note
Working concentration:
Antipain: 50 µg/ml (74 µM)
Bestatin: 40 µg/ml (130 µM)
Chymostatin: 6 - 60 µg/ml (10 - 100 µM)
E-64: 0.5 - 10 µg/ml (1.4 - 28.0 µM)
Leupeptin: 0.5 µg/ml (1 µM)
Pepstatin: 0.7 µg/ml (1 µM)
Phosphoramidon: 4 - 330 µg/ml (7 - 600 nM)
Pefabloc SC: 0.1 - 1 mg/ml (0.4 - 4 mM
EDTA: 0.2 - 0.5 mg/ml (0.5 - 1.3 mM)
Aprotinin: 0.06 - 2 µg/ml (0.01 - 0.3 µM).

The protease inhibitors in this set can be used separately or as cocktails. The effectiveness of protease inhibition can be quickly and sensitively verified with the Universal Protease Substrate (casein, resorufin-labeled). All protease inhibitors are also available individually (with the exception of EDTA); for detailed information, please refer to the product listing for the respective inhibitor.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solid
Торговая марка	Roche
mp	209-217 °C
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H312 + H332 - H314 - H371
pcodes	P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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Proteinase K, recombinant, PCR Grade

3.4.23.1 ( BRENDA | IUBMB )

Proteinase K is a subtilisin-related serine protease that has no pronounced cleavage specificity.
The enzyme is also available as a lyophilizate.
The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. The specifications of the recombinant enzyme are the same as those of the native protease. The amino acid sequence (molecular weight) and the molecule structure are identical. However, the recombinant preparation is much purer than the native enzyme, as it is DNA-free, according to the current quality control procedures, and is therefore well suited for isolating PCR and RT-PCR templates.

Features and Benefits
Proteinase K cleaves proteins between amino acids X and Y (X--Y), when X = an aliphatic, aromatic, or hydrophobic amino acid, and Y = any amino acid. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases.

This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
The enzyme promotes cell lysis by activating a bacterial autolytic factor.
Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.
Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Choose an effective tool for template preparation. Inactivate DNases and RNases of most species.
Count on consistent quality and performance. Stringent quality testing ensures optimal stability and high-level lot-to-lot performance.
Prepare samples over a wide range of conditions. The robust enzyme is stable over a wide pH range and is ideal for diverse applications.
Benefit from a contamination-free enzyme. The enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA. It is especially suited for the isolation of PCR templates.

Quality
This preparation is free of RNases, DNases, and DNA, according to the current quality control procedures.
Absence of Nucleases: Each lot is tested on various substrates to ensure the absence of endonuclease, exonuclease, ribonuclease, and nicking activity.
DNA Content: 10pg/mg enzyme (determined by Threshold)
Bioburden: 5cfu/g (determined by the most stringent test from the European Pharmacopoeia, which identifies the total number of viable aerobic bacteria, yeast, and fungi)
Unit Definition
Volume Activity: Approximately 50 U/ml solution (chromozyme assay); approximately 600 U/ml solution (hemoglobin assay). One unit is the enzyme activity which cleaves at +25 °C in 1 min 18 mmol Chromozym TRY (equivalent to 600 U/ml with the hemoglobin assay). Refer to the Certificate of Analysis for specific values for the present lot.
Preparation Note
Activator: To stimulate proteinase K activity, denaturing agents (SDS and urea) can be added. For example, SDS at a final concentration of 2% can increase the activity of proteinase K significantly. Optimization using denaturing agents can increase proteinase activity by as much as sevenfold.
Working solution: Suggested Buffers:
The most appropriate buffer for Proteinase K will vary from application to application. Always follow the pH and temperature guidelines in parameter filed. As a general rule, proteinase K is stable and very active in buffers that contain denaturing reagents such as urea, sodium dodecyl sulfate (SDS), and guanidinium salts.
Inhibitors: The enzyme is inactivated by Pefabloc® SC. However, it is not inactivated by metal ions, chelating agents (e.g., EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.

For the rapid inactivation of RNases and DNases.
The enzyme can reduce protein to free amino acids if it is present in large excess for long incubation periods.
For life science research only. Not for use in diagnostic procedures.

Pefabloc is a registered trademark of Pentapharm

Quality Level	100,
form	buffered aqueous solution (18 ± mg/mL; pH 7.5)
specific activity	~2.5 units/mg protein (Approximately 2.5 U/mg (Chromozym assay); 30 U/mg hemoglobin assay. Refer to the Certificate of Analysis for specific values for the present lot.)
packaging	pkg of 1.25 mL (03115887001), pkg of 25 mL (03115844001), pkg of 5 mL (03115828001)
Торговая марка	Roche
optimum pH	6.5 and 9.5
pH range	4.0 - 12.5
shipped in	wet ice
storage temp.	2-8°C

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PVDF Western Blotting Membranes 3010040001

PVDF Western Blotting Membranes are hydrophobic, microporous, polyvinylidene difluoride (PVDF) membranes with a pore size of 0.2µm. The PVDF Western Blotting Membranes are ideal for western and dot blots. The high binding capacity, high mechanical strength, and chemical resistance of the PVDF membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature.
Adsorption: Goat IgG: 294µg/cm2; BSA: 131µg/cm2; insulin: 85µg/cm2

PVDF Western Blotting Membranes has been used in western blot, for transferring proteins for sequencing.
Note: Tested for use with Lumi-Light western blotting substrate or Lumi-LightPlus western blotting substrate.
Quality
Each lot is function tested (dot blot).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
material	white PVDF membrane (hydrophobic)
packaging	roll of 1
Торговая марка	Roche
roll W × L	30 cm x 3.00 m
pore size	0.2 μm pore size
shipped in	ambient
storage temp.	room temp

Specificity
Pvu I recognizes the sequence CG°AT *CG and generates fragments with 3-cohesive termini.
Recognition sites: CG°AT*CG
CG°AT*CG
Restriction site: CG°AT*CG
CG°AT*CG
Heat inactivation: No inactivation of Pvu I after incubation at 65 °C for 15 minutes.
Quality
Absence of nonspecific endonuclease activities
1µg DNA is incubated for 16hours in 50µl SuRE/Cut Buffer H with an excess of Pvu I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Pvu I for 4hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs

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Pvu I

Кат. номер

10650137001

10650129001

: 3
X174: 0
Ad2: 7
M13mp7: 1
pBR322: 1
pBR328: 1
pUC18: 2
SV40: 0

Unit Definition
One Unit is the enzyme activity that completely cleaves 1 µg DNA in one hour at +37 °C in a total volume of 25 µl (1x) SuRE/Cut Buffer H.
Analysis Note
Activity in PCR buffer: <5%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is less than 5%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 µM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.
Compatible ends
Pvu I generates ends that are compatible with fragments generated by Pac I.
Isoschizomers
Pvu I is an isoschizomer to BspC I and Xor II.

Methylation sensitivity
Pvu I cleavage is not inhibited by overlapping dam-methylation at the site indicated (°) on the recognition sequence, but Pvu I fragments of DNA isolated from dam+ strains are not as readily religated as those isolated from dam- strains. Pvu I is inhibited by 5-methylcytosine at the indicated site (°) and by 4-methylcytosine.
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 50-75%
B: 75-100%
H: 100%
L: 25-50%
M: 50-75%

Incubation temperature
+37°C
Unit definition
One Unit is the enzyme activity that completely cleaves 1µg DNA in 1 hour at +37°C in a total volume of 25µl SuRE/Cut Buffer H.

Heat inactivation
Pvu I cannot be heat inactivated by incubating it for 15 minutes at +65°C.
PFGE tested
Pvu I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend 10U of enzyme/µg DNA and 4 hour incubation time.
Ligation and recutting assay
Pvu I fragments obtained by complete digestion of 1 µg pBR322 DNA are ligated with 1U T4 DNA Ligase in a volume of 10µl by incubation for 16hours at +4°C in 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C) resulting in >80% recovery of 1µg pBR322 DNA fragments.
Subsequent re-cutting with Pvu I yields >95% of the typical pattern of pBR322 DNA x Pvu I fragments.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 100 U (10650137001 [5 U/µl]), pkg of 500 U (10650129001 [5 U/µl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

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Pwo Master 3789403001

Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3–5 exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.

Use this premixed, double-concentrated formulation containing PwoSuperYield DNA Polymerase, a new optimized buffer system, and PCR grade nucleotides, if your application requires better fidelity than provided by (FastStart) Taq DNA Polymerase. The product can be applied for:

Accurate amplification of DNA critical applications
High-throughput PCR
High fidelity PCR
Site-directed mutagenesis
Cloning
Gene expression

Features and Benefits
Pwo Master is a ready-to-use, 2x concentrated master mix. Pwo SuperYield DNA Polymerase has polymerase and 3 5 exonuclease proofreading activity, including an optimized buffer. The buffer enhances enzyme performance, synthesizing higher DNA yields of high fidelity.

Higher yield and 18-fold higher fidelity

compared to Taq DNA Polymerase.

Maximize convenience.

Fewer steps are required with the ready-to-use master mix. Simply add template DNA, primers, and PCR-grade water then start the reaction.

Reduce setup time.

Store at +2 to +8°C - no need to thaw reagents.
Packaging
1 kit containing 2 components
Quality
Quality control is performed using human genomic DNA, with primers specific for tPA that generate a 1.7kb product.
Unit Definition
Volume Activity: 2.5.U - 50 μl reaction

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Quality Level	100,
usage	sufficient for ≤100 reactions
packaging	pkg of 2.5 mL (10 x 250 μl)
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Pwo SuperYield DNA Polymerase, dNTPack 4743750001

2.7.7.7 ( BRENDA | IUBMB )

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction without changing the fidelity of DNA synthesis. This enzyme delivers excellent results due to its enzyme design and optimized buffer system. It amplifies fragments up to 3 kb - even longer amplicons are possible from simple templates.
Pwo DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5 3 DNA polymerase with 3 5 exonuclease proofreading activity; 5 3 exonuclease activity is not detectable.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5 mM.
Specificity
Star activity: In cases where star activity is observed and/or the activity of the enzyme in the PCR mix is low, purify the amplification product prior to the restriction enzyme digest using High Pure PCR Product Purification Kit.

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for:

High fidelity PCR
Site-directed mutagenesis
Cloning
Gene expression
Study of allelic polymorphism in individual RNA transcripts
Characterization of rare mutations in tissue
Characterization of the allelic stage of single cells or single DNA molecules

Features and Benefits
Pwo SuperYield DNA Polymerase yields more high fidelity PCR product. The optimized buffer delivers superior results. Amplify fragments up to 3 kb. A GC-RICH Solution is included for difficult templates. Each dNTPack contains the 10 mM additive-free sodium salt nucleotides in a ready-to-use mix.

Higher yield and 18-fold higher fidelity

compared to Taq DNA Polymerase.

High performance with difficult templates.

The GC-RICH Solution is for GC-rich PCR.

Reduce working steps in cloning.

Perform digests directly in Pwo SuperYield PCR mix.

Cost-effective.

Use the convenient premixed solution of PCR grade dNTPs.
Packaging
1 kit containing 4 components
Quality
Each lot is assayed using activated DNA. PCR testing used λ and human genomic DNA.
Unit Definition
One unit Pwo SuperYield DNA Polymerase is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol total deoxynucleoside triphosphates into acid precipitable DNA within 30 minutes at +70 °C.
Unit Assay: Incubation buffer for assay on activated DNA
20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [-32P]dCTP are incubated with 0.01 to 0.1 U Pwo SuperYield DNA Polymerase in 50 µl incubation buffer with a paraffin oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/µl

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤40 reactions
Quality Level	100,
packaging	pkg of 100 U
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Pyrophosphatase, inorganic (PPase) 10108987001

3.6.1.1 ( BRENDA | IUBMB )

The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.
Pyrophosphate phosphohydrolase
Specificity
Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :

Enzymatic nitric oxide (NO) detection system
In vitro transcription
Multiply-primed rolling circle amplification

Biochem/physiol Actions
Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.
Quality
Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	yeast
form	suspension
specific activity	~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)
packaging	pkg of 1 mL (1 mg)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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Pyruvate Kinase (PK)

2.7.1.40 ( BRENDA | IUBMB )

ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.

Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.
Biochem/physiol Actions
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.
Quality
Contaminants: <0.001% GK, <0.002% “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.)
packaging	pkg of 1 mL (10128155001 [10 mg]), pkg of 10 mL (10128163001 [100 mg])
Торговая марка	Roche
optimum pH	7.0-7.5
shipped in	wet ice
storage temp.	2-8°C

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Pyruvate Kinase (PK) 10109045001

2.7.1.40 ( BRENDA | IUBMB )

Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.
Specificity
Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.

Pyruvate Kinase (PK) has been used in the kinase assay.
Pyruvate Kinase (PK) has been used as a sample in ATPase Assays.
Biochem/physiol Actions
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.
Quality
Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.
Physical form
Solution in 50% glycerol (v/v), pH approximately 6
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~200 units/mg protein (At 25 °C with PEP as the substrate.)
packaging	pkg of 1 mL (10 mg)
Торговая марка	Roche
optimum pH	7.0-7.5
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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rAPid Alkaline Phosphatase

3.1.3.1 ( BRENDA | IUBMB )

rAPid Alkaline Phosphatase is an alkaline phosphatase isolated from bovine intestine and supplied as a recombinant enzyme of 56 kD, expressed in the yeast, Pichia Pastoris. The recombinant form ensures consistency and safety. rAPid Alkaline Phosphatase catalyzes the dephosphorylation of 5 phosphates from DNA and RNA, nucleotides, and proteins. Alkaline phosphatase treatment prevents self-ligation of DNA fragments by removing the 5-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. Unlike calf intestinal phosphatase, rAPid Alkaline Phosphatase is rapidly, completely, and irreversibly inactivated by heat treatment for two minutes at +75 °C. It is therefore, an excellent alternative to Shrimp Alkaline Phosphatase. In addition, the enzyme is active in restriction enzyme buffers. Therefore, restriction enzyme digestion, dephosphorylation, enzyme inactivation, ligation, or 5-end labeling can be performed without purification steps.
Specificity
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rAPid Alkaline Phosphatase is inactivated by incubation at 75 °C for two minutes.

Note: rAPid Alkaline Phosphatase is very rapidly inactivated by heating at +75 °C for two minutes. No additional purification steps are required after restriction and dephosphorylation. The dephosphorylated DNA can directly be used in a ligation reaction.
Note: rAPid Alkaline Phosphatase is also included in the Rapid DNA Dephos & Ligation Kit, which enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. With this convenient kit, dephosphorylation of blunt and sticky 5-overhanging ends is performed in ten minutes at +37 °C and ligation is completed in just five minutes at +15–25 °C.
Features and Benefits

Use rAPid Alkaline Phosphatase in numerous applications, including: Removal of 5-phosphoryl groups from nucleic acids
Preparation of templates for 5-end labeling
Clean-up of PCR products by removal of dNTPs
Dephosphorylation of proteins

Eliminate contamination risk: Recombinant expression, and reliable and sensitive quality control tests ensure high purity.
Replace your current phosphatases with an economical solution: rAPid Alkaline Phosphatase performs equally as well as Shrimp Alkaline Phosphatase or Bovine Alkaline Phosphatase.
Eliminate the risk of animal-derived diseases: Expression in Pichia pastoris ensures consistency and safety.
Benefit from rapid heat inactivation: rAPid Alkaline Phosphatase is inactivated after only 2 minutes at +75°C.
Obtain consistent performance: rAPid Alkaline Phosphatase has excellent stability during storage compared to other alkaline phosphatases.

Packaging
1 kit containing 2 components
Quality
All lots of rAPid Alkaline Phosphatase are tested for the absence of deoxyribonucleases and nicking activity according to the current Quality Control procedures.
Unit Definition
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes 1 mol of 4-nitrophenyl phosphatase in one minute at +37 °C under assay conditions.
Volume Activity: 1 U/µl

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
specific activity	1 units/μg protein
packaging	pkg of 1,000 U (04898133001 [1 U/μl]), pkg of 5,000 U (04898141001 [1 U/μl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	−20°C

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Rapid DNA Dephos & Ligation Kit

3.1.3.1 ( BRENDA | IUBMB )

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Specificity
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:

Cloning of fragments into either plasmid or phage vectors
Linker ligation
Recircularization of linear vector DNA
Generation of libraries

Packaging
1 kit containing 5 components
Unit Definition
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one µmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/µl
T4 DNA Ligase: 5 U/µl

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001)
Quality Level	100,
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	−20°C

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Restriction Protease Factor Xa

3.4.21.6 ( BRENDA | IUBMB )

Special quality for the cleavage of fusion proteins.
Lyophilizate
Specificity
The coagulation factor Xa is a serine protease which recognizes the amino acid sequence Ile—Glu—Gly—Arg ↓ with a high degree of specificity. Cleavage of this sequence activates the natural substrate prothrombin to thrombin. This sequence can also be used as a restriction cleavage site for processing of recombinant fusion proteins. When a lack of specificity is due to steric hindrance, the introduction of an additional amino acid sequence can sometimes overcome the problem.

Processing of fusion proteins at definite cleavage site. The restriction protease factor Xa is a serine protease which recognizes the amino acid sequence Ile — Glu — Gly — Arg ↓ with a high degree of specificity. This sequence may also be used as a restriction cleavage site for processing of recombinant fusion proteins.
Quality
Purity: highly purified (SDS-PAGE); no albumin added
Quality control: function tested
Inhibitors: APMSF, antithrombin III-heparin, DFP, PMSF, soybean trypsin inhibitor
Preparation Note
Working concentration: 1:10 to 1:200 (w/w)
A useable working concentration has a substrate ratio of 1:10 to 1:200 (w/w).

Note: The contents of a vial may be used for several simultaneous digests. When repeating the digest, a new vial should be used. Following this procedure ensures reproducibility and prevents possible contamination.
Storage conditions (working solution): A solution in redistilled water can be used for up to one week when stored at 4 °C.
An aqueous solution, stored in aliquots at -20 °C is stable for approx. one month.
Freezing aliquots increases enzyme stability in solution.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
mol wt	(M_r = 43 kD)
packaging	pkg of 3 x 100 µg (11585924001), pkg of 3 x 250 µg (11179896001)
Торговая марка	Roche
optimum pH	8.3
shipped in	wet ice
storage temp.	2-8°C

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Reverse Transcriptase Assay, colorimetric 11468120910

Colorimetric enzyme immunoassay for the quantitative determination of retroviral reverse transcriptase activity by incorporation of digoxigenin- and biotin-labeled dUTP into DNA.

The Reverse Transcriptase Assay is designed for the quantitative determination of RT activity in cell culture and other biological samples. The assay is used to determine the propagation of retroviruses in retrovirus-infected mammalian cells in culture. The assay is also used for in vitro screening for RT inhibitors.
Packaging
1 kit containing 14 components.
Specifications
Assays for reverse transcriptase (RT) activity have been broadly applied for testing retroviral propagation in vitro. RT is required for early proviral DNA synthesis, and is thus a prime target for anti-retroviral therapy, for example, in the case of acquired immunodeficiency syndrome (AIDS). Reverse transcriptase generally uses RNA, which is complexed with various primers, as a template for DNA synthesis. Classically, for the detection or quantification of RT activity, the amount of incorporated radioactively labeled nucleotides is measured.
Assay time: Approximately 4 hours (for an enzyme reaction of 1 hour), approximately 18 hours (for an enzyme reaction of 15 hours).
Sample material: Cell culture supernatant and other biological samples, RT inhibitors.
Sensitivity: The Reverse Transcriptase Assay detects 20pg HIV-1 reverse transcriptase in a 1-hour reaction. One pg can be detected in an overnight reaction.
The assay detects the activity of natural or recombinant retroviral reverse transcriptase, including that of HIV-1, HIV-2, SIV-1, AMV, and M-MulV.
Principle
The Reverse Transcriptase Assay, colorimetric, takes advantage of the ability of reverse transcriptase to synthesize DNA using the hybrid poly (A) x oligo (dT)15 as a template and primer. It avoids the use of [3H]- or [32P]-labeled nucleotides that are employed in standard RT assays. In place of radiolabeled nucleotides, digoxigenin- and biotin-labeled nucleotides in an optimized ratio are incorporated into the same DNA molecule by the RT activity. A template/primer hybrid is supplied, but the flexibility of the assay allows the use of a template of choice (e.g., a viral template). The detection and quantification of the synthesized DNA as a parameter for RT activity follows a sandwich ELISA protocol: biotin-labeled DNA binds to the surface of streptavidin-coated microplate modules. In the next step, an antibody to digoxigenin, conjugated to peroxidase (anti-DIG- POD), is added and bound to the digoxigenin-labeled nucleotides (Licensed by Institute Pasteur). In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate to produce a colored reaction product. The absorbance of the samples is determined using a microplate (ELISA) reader, and is directly correlated to the level of RT activity in the sample.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤200 tests
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317 - H319 - H412
pcodes	P261 - P273 - P280 - P333 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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RNase 10109134001

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.
Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Specificity
Heat inactivation: 1 mM EDTA

RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.
Unit Definition
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	dry powder (prepared from crystallized ribonuclease)
specific activity	~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)
packaging	pkg of 500 mg
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

Sma I recognizes the sequence *C°C*CGGG and generates fragments with blunt ends.
Specificity
Recognition sites: *C °C*CGGG
*C °C*CGGG
Restriction site: *C °C*CGGG
*C °C*CGGG
Heat inactivation: Sma I can be heat inactivated by incubation at 65 °C for 15 minutes (up to 100 U/µg DNA).

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Sma I

Sma I has been used in macrorestriction analysis. It has also been used in the restriction enzyme mixture during restriction digestion, amid rapid pulsed-field gel electrophoresis (PFGE).
Quality
Absence of nonspecific endonuclease activities
1µg DNA is incubated for 16 hours in 50µl SuRE/Cut Buffer A with an excess of Sma I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Sma I for 4 hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs

: 3
X174: 0
Ad2: 12
M13mp7: 0
pBR322: 0
pBR328: 0
pUC18: 1
SV40: 0

Unit Definition
One unit is the enzyme activity that completely cleaves 1 µg DNA in one hour at +25 °C in a total volume of 25 µl (1x) SuRE/Cut buffer A.
Analysis Note
Compatible ends
Sma I generates ends that are compatible with any blunt end.

Isoschizomers
The enzyme is an isoschizomer to Cfr9 I, PspA I, Xma I, and XmaC I.
Methylation sensitivity
Sma I is not inhibited by 5-methylcytosine at the middle of the three C residues (°) in the recognition sequence. However, the activity is inhibited by 5-methylcytosine at the other Cs (*) or 4-methylcytosine in any position within the recognition sequence (*C°C*CGGG).
Incubation temperature
+25°C
PFGE tested
Sma I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend 10 U of enzyme/µg DNA and 4 hour incubation time.

Ligation and recutting assay
Sma I fragments obtained by complete digestion of 1µg DNA are ligated with 1U T4 DNA Ligase in a volume of 10µl by incubation for 16 hours at +25°C in 66mM Tris-HCl, 5mM MgCl2, 5mM Dithioerythritol, and 1mM ATP, pH 7.5 (at +20°C) resulting in >80% recovery of 1µg DNA fragments.
Subsequent re-cutting with Sma I yields >80% of the typical pattern of DNA x Sma I fragments.
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 100%
B: 0-10%
H: 0-10%
L: 0-10%
M: 0-10%

Activity in PCR buffer: 100%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 µM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 100%. If supplemented with GC-RICH Solution, activity remains at 100%. Pwo SuperYield DNA Polymerase PCR Mix is not available in U.S.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1,000 U (10220566001 [10 U/µl]), pkg of 5,000 U (10656348001 [10 U/µl]), pkg of 5,000 U (11047639001 [40 U/µl])
Торговая марка	Roche
parameter	25 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

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Sorbitol Dehydrogenase (SDH) 10109339001

1.1.1.14 ( BRENDA | IUBMB )

L-iditol:NAD+ 5-oxidoreductase
Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis.

Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses).
Biochem/physiol Actions
Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of D-sorbitol to D-fructose and forms a part of the sorbitol pathway, which is a crucial bypass to glycolysis for glucose metabolism. This pathway has been found to be associated with the accumulation of sorbitol in lens, which results in diabetic cataractogenesis. SDH also oxidizes various polyols and other secondary alcohols into their corresponding ketones.
Quality
Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each.
Unit Definition
One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed.
Physical form
Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose).
Preparation Note
Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer.
Stabilizers: Maltose is used as stabilizer.
Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks.
Storage and Stability
Store at 2 to 8 °C. (Store the lyophilizate dry.)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~40 units/mg protein (At 25 °C with D-fructose as the substrate.)
packaging	pkg of 10 mg (60 mg lyophilizate)
Торговая марка	Roche
optimum pH	7.5
shipped in	wet ice

Spe I recognizes the sequence *A*CTAGT and generates fragments with 5-cohesive termini.
Specificity
Recognition sites: *A*CTAGT
*A*CTAGT
Restriction site: *A*CTAGT
*A*CTAGT
Heat inactivation: Spe I can be heat inactivated by incubation at 65 °C for 15 minutes (up to 100 U/µg DNA).
Quality
Absence of nonspecific endonuclease activities
1µg Ad2 DNA is incubated for 16 hours in 50µl SuRE/Cut Buffer H with an excess of Spe I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Spe I for 4 hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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SP6 RNA Polymerase
Кат. номер

11487671001

10810274001

General description
With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [-32P] or [-35S] labeled nucleotides.
Contents:
SP6 RNA Polymerase, 20 U/µl, in buffer, pH 7.9
Transcription Buffer, 10x concentrated

Specificity
Promoter specifity
SP6 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage SP6 DNA or DNA cloned downstream of a SP6 promoter (e.g., pSPTBM20; pSPTBM21).
Heat inactivation: Stop the reaction by adding 2 µl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.
Application
SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:
RNA or DNA blotting techniques
In situ hybridization
RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
The synthesis of antisense RNA probes

Quality
Test Buffer
40 mM Tris-HCl, pH 8.0 (+20°C), 6 mM MgCl2, 10 mM dithiothreitol, 2 mM spermidine, pH approximately 8.0 (+20°C).
Absence of Endonucleases
1. 1 µg lambda DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 µl test buffer. The number of enzyme units which show no degradation of lambda DNA is > 30 U.
2. 1 µg Eco RI/Hind III fragments of lambda DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 µl test buffer. The number of enzyme units which show no alteration of the banding pattern is > 30 U.
Absence of Nicking Activity
1 µg pBR322 DNA is incubated with SP6 RNA polymerase for 4 hours at +37°C in 25 µl test buffer. The number of enzyme units which show no relaxing of supercoiled structure is > 30.
Absence of RNases
4 µg MS2 RNA are incubated with SP6 RNA polymerase for 4 hours at +37°C in 50 µl test buffer. The number of enzyme units which show no degradation of MS2 RNA is > 30.
Performance in Transcription Assay
SP6 RNA polymerase is function tested in the SP6/T7 Transcription Kit (Cat. No. 10 999 644 001). The incorporation rate in the standard assay with 0.5 µg pST18 neo DNA linearized with Eco RI and 50 mCi [alpha-32P] CTP, [400 Ci/mmol (15 TBq/mmol)] gives >50% of the input radioactivity in 20 minutes.
Specifications
Synthesis of hybridization probes: SP6 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.
Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with SP6 RNA Polymerase.
Unit Definition
One unit is the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA products within 60 minutes at +37 °C.
Volume Activity: 20 U/µl
Preparation Note
Activator: BSA/spermine
Other Notes
For life science research only. Not for use in diagnostic procedures.
Параметры

Quality Level 100,

form solution

packaging pkg of 1,000 U (10810274001), pkg of 5,000 U (11487671001)

Торговая марка Roche

shipped in dry ice

storage temp. 20°C

Safety Information

RIDADR NONH for all modes of transport
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Spe I

: 0
X174: 0
Ad2: 3
M13mp7: 0
pBR322: 0
pBR328: 0
pUC18: 0
SV40: 0

Unit Definition
One unit is the enzyme activity that completely cleaves 1 µg Ad2 DNA in one hour at +37 °C in a total volume of 25 µl SuRE/Cut Buffer H.
Analysis Note
Compatible ends
Spe I ends are compatible with ends generated by Bln I, Nhe I, and Xba I.
Isoschizomers
The enzyme is an isoschizomer of Bcu I and Ahl I.
Methylation sensitivity
As indicated by (*) on the recognition sequence above, Spe I is inhibited by the presence of N6-methyladenine and 5-methylcytosine ( mAm CTAGT).
Incubation temperature
+37°C
PFGE tested
Spe I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E. coli C 600) embedded in agarose for PFGE analysis, we recommend using 10U of enzyme/µg DNA and 4 hour incubation time.
Ligation and recutting assay
Spe I fragments obtained by complete digestion of 1µg Ad2 DNA are ligated with 1U T4 DNA Ligase in a volume of 10µl by incubation for 16 hours at +4°C in 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C), resulting in >90% recovery of Ad2 DNA.
Subsequent re-cutting with Spe I yields >95% of the typical pattern of Ad2 x Spe I fragments.

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 75-100%
B: 75-100%
H: 100%
L: 75-100%
M: 100%

Activity in PCR buffer: Not tested

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1,000 U (11008951001 [10 U/µl]), pkg of 1,000 U (11207644001 [40 U/µl]), pkg of 200 U (11008943001 [10 U/µl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).
Sequence
Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.
Unit Definition
One unit binds 1 μg D-biotin at pH 8.9.
Physical form
White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A282 = 3.1)
KD: 10-15M
Reconstitution
Add 1 ml double-distilled water to get a final concentration of 1 mg/ml (11 721 666 001) or 5 mg/ml (11 721 674 001), respectively.

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3708152001
Streptavidin Mutein Matrix 3708152001
General description
Streptavidin mutein, immobilized.
Contents
Settled resin of 50% suspension Streptavidin Mutein Matrix.
The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(Kd 0.6 x 10-15 M (avidin), 4 x 10-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Application
Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
Is convenient for purification of chemically biotinylated proteins
It was also used in single-step affinity chromatography and affinity chromatography.

Features and Benefits
Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
Economic: High binding capacity and multiple reuse allows economic handling of the matrix.

Storage and Stability
Store at 2 to 8 °C. (Do not freeze!)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Параметры

form suspension

packaging pkg of 5 mL

Торговая марка Roche

parameter 4-25 °C optimum reaction temp.

shipped in wet ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany WGK 1

Flash Point F does not flash

Flash Point C does not flash
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Streptavidin, recombinant

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	17 units/mg protein
mol wt	~52 kDa
packaging	pkg of 1 mg (11721666001), pkg of 5 mg (11721674001)
Торговая марка	Roche
pH range	5.0 - 9.0
pI A	7.4-7.7 (two main bands)
solubility	water: 10 mg/L (mA₄₀₅=100)
shipped in	wet ice
storage temp.	2-8°C

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Streptavidin--Gal conjugate 11112481001

Streptavidin is covalently coupled to -galactosidase from E. coli.

Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

Use Streptavidin--Gal to detect biotinylated compounds using: ELISA
Immunohistocytochemistry
Western blot
In situ hybidization

ELISA: 100 to 500 mU/ml
Immunohistocytochemistry: 250 to 500 mU/ml
Western blot: 250 to 500 mU/ml

Storage conditions (working solution): The reconstituted solution is stable at 2 to 8 °C for four weeks. Aliquots should be stored at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 500 U/ml.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized (clear, colorless solution after reconstitution)
packaging	pkg of 500 U
Торговая марка	Roche
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H317 - H412
pcodes	P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Streptavidin-AP conjugate 11089161001

Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.
Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.

Streptavidin-AP has been used to detect biotinylated compounds using:

ELISA
Immunohistocytochemistry
Western blot

Physical form
Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.
Preparation Note
Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.
ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)
Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
packaging	pkg of 1 mL (1,000U)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

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11093266910

Streptavidin-AP-conjugate for nucleic acid detection 11093266910

Application
For the detection of biotin-labeled nucleic acids.
Physical form
Solution in 50 mM triethanolamine buffer, 3M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.
Preparation Note
Working Solution:
Prepare by diluting the conjugate solution 1:5,000 with 100 mM Tris-HCl, 150mM NaCl, pH 7.5
Other Notes
For life science research only. Not for use in diagnostic procedures.
Параметры

form solution

Quality Level 100,

packaging pkg of 200 μL (150U)

Торговая марка Roche

shipped in wet ice

storage temp. 2-8°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany WGK 1

Flash Point F does not flash

Flash Point C does not flash

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Streptavidin-POD conjugate 11089153001

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Streptavidin-POD has been used for:

ELISA
In situ hybridization
Western blot

It is also used for immunohistochemistry.
Unit Definition
One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 µmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Physical form
Lyophilizate, stabilized
Preparation Note
Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted). Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:

ELISA: 5 to 50 mU/ml
Immunohistocytochemistry: 250 to 500 mU/ml
Western blot: 100 to 250 mU/ml

Reconstitution
Add 1 ml double-distilled water to a final concentration of 500 U/ml.
The reconstituted stock solution is stable at 2 to 8 °C for 6 months.
Do not freeze reconstituted antibody! Do not add sodium azide!
A further dilution of reconstituted stock solution in double-distilled water is stable for approx. one week at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 500 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

Transparent, streptavidin-coated microplates with high binding capacity:

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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StreptaWell™, High Bind (transparent, 12 x 8-well strips)

Blocked volume: >300 µl
SA-coated area: 300 µl
Well geometry: C

Total biotin binding capacity: 20ng/well, 80pmol/well (competition assays; 300µL wells)
Total binding capacity: 1.5µg/well (biotin-labeled antibodies)

StreptaWell™ can be used for colorimetric and fluorescent protein immunoassays.
Features and Benefits
The unique coating process ensures homogenous and reproducible coatings with unique features:

Little variances between individual wells (intra-assay variance)
Little variances between different plates (inter-assay variance)
High lot-to-lot reproducibility
High signal-to-noise ratio
Low background
Almost no leaching

Quality
SA leaching (bleeding): <5ng/well

For life science research only. Not for use in diagnostic procedures.

StreptaWell is a trademark of Roche

Quality Level	100,
packaging	pkg of 15 plates (11989685001 [96 wells]), pkg of 5 plates (11645692001 [12 x 8 well strips and frame])
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3-hydroxyl- and 5-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
Contents
T4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
Specificity
Heat inactivation: The enzyme can be inactivated by heating (65 °C, 10 minutes).

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T4 DNA Ligase

Ligation of sticky- and blunt-ended DNA fragments.
Quality
Quality
Tested for the absence of deoxyribonucleases and exonucleases, according to the current Quality Control procedures.
Unit Definition
One unit of T4 DNA ligase is the amount of enzyme activity that converts 1 nmol 32P from pyrophosphate into Norit-absorbable material in 20 minutes at +37 °C. One unit corresponds to 0.2 U determined in the exonuclease III resistance assay. Dilution buffer: 50 mM Tris-HCl, 10 mM dithioerythritol, bovine serum albumin, 500 µg/ml, pH 7.6 (+25 °C).
Volume Activity: 1 x 103 U/ml; 5 x 103 U/ml
Preparation Note
Activator: Mg2+ (10mM), DTT
Working solution: For dilution of the enzyme Roche recommends using a buffer containing the components of the storage buffer: 20 mM Tris-HCl, 60 mM KCl, 1 mM EDTA, 5 mM dithoerythritol, 50% glycerol (v/v), pH 7.5 (4 °C).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	~3000 units/mg protein
packaging	pkg of 100 U (10481220001 [1 U/μl]), pkg of 500 U (10716359001 [1 U/μl]), pkg of 500 U (10799009001 [5 U/μl])
Торговая марка	Roche
parameter	4-22 °C optimum reaction temp.
optimum pH	7.2-7.8
shipped in	dry ice
storage temp.	−20°C