Roche

2.7.1.1 ( BRENDA | IUBMB )

ATP:D-hexose 6-phosphotransferase
Hexokinase exists in three isozymes, namely type I, type II and type III that differ in subcellular localization, catalytic and regulatory properties. The type I isozyme serves a catabolic function facilitating the entry of glucose into glycolytic metabolism for energy production. The type II and type III enzymes perform anabolic functions, such as generating G6P for glycogen synthesis or metabolism through the phosphogluconate pathway for lipid synthesis. Type I and type II isozymes have been found to be bound to mitochondria. The type III isozyme has been found to have perinuclear localization in several cell types.
Hexokinase consists of 486 amino acids and has a molecular weight of 57,000 D (SDS-PAGE) in citrate/phosphate buffer. It may form dimers under other buffer conditions.

Hexokinase (HK) has been used in enzyme assays and chemiluminescence assay. It has also been used to determine ATP concentration.
Use Hexokinase for the determination of D-glucose, D-fructose, and D-sorbitol in food or biological research samples. Hexokinase may also be used for the assay of other saccharides, which are convertible to glucose or fructose, and is, therefore, useful in the assay of many glycosides.
Hexokinase has been used in the enzymatic detection of nitric oxide and in the measurement of ATP by luminescence method.
Biochem/physiol Actions
Hexokinase catalyzes the conversion of hexoses to hexose-6-phosphate in an ATP-dependent manner. It is also called as the glycolytic enzyme as it catalyzes the first step in glucose metabolism with the formation of glucose-6-phosphate (G6P). In eukaryotes, it functions as a glucose sensor.
Quality
Contaminants: <0.002% PGI, <0.0005% G6P-DH and GR, each, <0.001% CK, MK, 6-PGDH, and ADH, each, <0.05% GIDH and invertase (-fructosidase), each, <30 µg/ml glucose (enzymatically)
Unit Definition
Unit Assay: For measuring hexokinase activity 0.22 M glucose in 0.1 M triethanolamine buffer, pH 7.6, 6.5 mM MgCI2, 2.7 mM ATP, 0.83 mM NADP is incubated in the presence of 1.7 U glucose-6-phosphate dehydrogenase with an appropriate amount of hexokinase (10–20 mU) at +25 °C (total volume: 3.0 ml). The enzyme activity is calculated from the increase of absorbance at 340 nm ( = 6.3 [I x mmol/l-1 x cm-1]) or 365 nm ( = 3.5 [I x mmol/l-1 x cm-1]).
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.5
Preparation Note
Activator: Hexokinase requires Mg2+ ions (Km = 2.6 mM) for activity. Catecholamines too increase activity.
Stabilizers: Thiols

For life science research only. Not for use in diagnostic procedures.

form	suspension
Quality Level	100,
specific activity	~450 units/mg protein (At 25 °C and pH 7.6 with D-glucose and ATP as the substrates.)
packaging	pkg of 1 mL (1,500 U), pkg of
Торговая марка	Roche
pH range	6.0 - 8.0
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

Кат. номер

10737275001

10127825001

General description
Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.
Application
Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.
Components
EC 2.7.1.1 & 1.1.1.49
Unit Definition
One unit (U) HK will phosphorylate 1 µmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 µmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 µmol of NADH per µmol of D-glucose phosphorylated.
Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.
Other Notes
For life science research only. Not for use in diagnostic prodcedures.
Параметры

Quality Level 100,

form suspension

packaging pkg of 10 mL (10737275001 [30 mg]), pkg of 5 mL (10127825001 [15 mg])

Торговая марка Roche

shipped in wet ice

storage temp. 2-8°C

Safety Information

RIDADR NONH for all modes of transport

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hGH ELISA 11585878001

The hGH assay system differs from most of the other commonly used reporter proteins (CAT, -Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g., RNA isolation). Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells.
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.
Human growth hormone (hGH) is specifically detected by the hGH ELISA. It does not cross-react with rat growth hormone. Cross-reactivity with the human homologues TSH, FSH, and LH has not been tested. The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
hGH Standards
The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
Specificity
The hGH ELISA specifically detecs human growth hormone (hGH). It does not cross-react with rat growth hormone.

For research use only. Not for use in diagnostic procedures.
The hGH ELISA is used to quantitatively measure the expression level of hGH released into cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene that encodes for hGH.
Features and Benefits

Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used
More sensitive: The hGH ELISA is approximately 20-times more sensitive than isotopic hGH assays
More specific: The hGH ELISA shows no crossreactivity with rat growth hormone
Faster: Measures hGH expression as early as 18hours post-transfection and requires only approximately 4 hours from start to finish
Easy to perform: Follows a standard ELISA protocol

Packaging
1 kit containing 9 components.
Specifications
Assay time: Approximately 4 hours
Sample material: Culture supernatant
Sensitivity: 5pg/ml (1pg/well)
Standards: The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
Principle
The hGH ELISA is based on the sandwich ELISA principle. Antibodies to hGH (anti-hGH) are prebound to the surface of the microplate. The culture supernatant, which contains secreted hGH, is added to the wells of the microplate modules. All hGH contained in the medium binds to the anti-hGH bound to the microplate surface. Next, a digoxigenin-labeled antibody to hGH (anti-hGH-DIG) is added, then bound to hGH. In the following step, an antibody to digoxigenin conjugated to peroxidase (anti-DIG-POD) is added, then bound to digoxigenin. In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate, yielding a colored reaction product. The absorbance of the sample is determined using an ELISA reader, and is directly correlated to the level of hGH present in the medium supernatant. The sensitivity of the assay can be enhanced using the peroxidase substrate (ABTS) with substrate enhancer.
Preparation Note
Working solution: Solution 1 hGH Stock Solution (bottle 1)
Reconstitute the lyophilizate in 0.5 ml double distilled water. The resulting concentration is calculated using the lot-specific information, given on the label (final conc. approx 10 ng/ml). This solution is used for the preparation of hGH standards for establishing a hGH calibration curve (see 7.2: preparation of hGH standards).
Solution 2 Anti-hGH-DIG (bottle 2)
Reconstitute the lyophilizate in 0.5 ml double distilled water (final conc. 100 µg/ml).
Solution 2a Anti-hGH-DIG, working dilution
To prepare anti-hGH-DIG working dilution, dilute the reconstituted anti-hGH-DIG solution (100 g/ml) with sample buffer (solution 7) to a final conc. of 1 µg/ml (e.g., 100 µl of reconstituted anti-hGH-DIG solution 9.9 ml of solution 7 for 50 wells).The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
Reconstitute the lyophilizate in 0.5 ml double distilledwater (final conc. 20 U/ml). Do not add sodiumazide!
Solution 3a Anti-DIG-POD, working dilution
To prepare anti-DIG-POD, working dilution, dilute the reconstituted anti-DIG-POD solution (20 U/ml) with sample buffer (solution 7) to a final conc. of 200 mU/ml (e.g., 100 µl of reconstituted anti-DIG-POD solution 9.9 ml of solution 7 for 50 wells). The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
Ready-to-use solution.
Solution 5 POD Substrate containing Substrate Enhancer (bottles 4 and 5)
If a low hGH concentration in the sample is expected, add 1 mg of substrate enhancer (bottle 5) per ml of solution 4 and mix by stirring for 30 min at 15 to 25 °C. The solution is stable for only 2 hours and should therefore be prepared immediately before use. Use the substrate enhancer only if the hGH concentration is low!
Solution 6 Washing Buffer, 1x (bottle 6)
To prepare a ready-to-use washing buffer, mix 1 part 10x washing buffer concentrate (bottle 6) with 9 parts of double distilled water.
Solution 7 Sample Buffer (bottle 7)
The solution is ready-to-use. Mix thoroughly before use. Do not add sodium azide.
Storage conditions (working solution): Solution 1 hGH Stock Solution (bottle 1)
The reconstitutedsolution is stable for 2 months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2 Anti-hGH-DIG (bottle 2)
The reconstituted solution is stable for two months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.
Solution 2a Anti-hGH-DIG, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 3 Anti-DIG-POD (bottle 3)
The reconstituted solution is stable for 6 months at 2 to 8 °C. Do not freeze!
Solution 3a Anti-DIG-POD, working dilution
The working dilution should be prepared freshly before use and should not be stored.
Solution 4 POD Substrate (bottle 4)
The solution is stable until the expiration date given on the label if stored at 2 to 8 °C.
POD Substrate containing Substrate Enhancer (bottles 4 and 5)
The solution is stable for only 2 hours and should therefore be prepared immediately before use.
Solution 6 Washing Buffer, 1x (bottle 6)
The reconstituted solution is stable for 6 months at 2 to 8 °C.
Solution 7 Sample Buffer (bottle 7)
After opening the bottle we recommend storing the solution in aliquots at -15 to -25 °C, since it does not contain a preservative agent.
Analysis Note
No cross-reaction with rat growth hormone. Cross reactivity with the human homologues TSH, FSH, and LH has not been tested.

For life science research only. Not for use in diagnostic procedures.

High Pure FFPE RNA Micro Kit 4823125001

Low to medium throughput isolation of total RNA from free FFPE tissue sections in micro scale.

The High Pure FFPE RNA Micro Kit isolates total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in:

Qualitative RT-PCR
Relative quantification of mRNA with real-time PCR systems such as the LightCycler® 480 System
Differential display RT-PCR
cDNA synthesis
Primer extension

Features and Benefits
The High Pure FFPE RNA Micro Kit isolates total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in RT-PCR.

Streamline and simplify RNA isolation (even small RNA fragments) from FFPE tissue.
Obtain a highly concentrated, ready-to-use eluate and excellent recovery of RNA (>80%).
Isolate DNA-free RNA for use in qualitative and quantitative RT-PCR.
Minimize RNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade RNA.
Generate high-quality template RNA that shows excellent performance and linearity in RT-PCR.

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA.
Size Distribution: The typical size of RNA isolated from formalin-fixed tissue ranges from 150 to 1500 bases. However, section thickness, tissue type, age of sample, and the fixation protocol used can affect the yield and quality of the isolated RNA.
Capacity: The High Pure Micro Filter Tubes hold up to 500 µl sample volume.
Sample Material: 1 - 10 µm sections from formalin-fixed, paraffin-embedded (FFPE) tissue (e.g., from colon, breast, liver, kidney, spleen of mammalian species).
Components

Tissue Lysis Buffer
Proteinase K, recombinant, PCR Grade
Binding Buffer
Wash Buffer I
Wash Buffer II
DNase I, recombinant, lyophilized
DNase Incubation Buffer
Elution Buffer
High Pure Micro Filter Tubes
Collection Tubes

Quality
Formalin-fixed, paraffin-embedded tissue sections are homogenized by overnight Proteinase K digestion and purified as described. RNA yield is determined by measuring the optical density at 260 nm.
The RNA eluate and specific primers for the 2M gene are used in one-step RT-PCR. In the following PCR on the LightCycler® 2.0 Instrument (accomplished using the LightCycler® RNA Amplification Kit SYBR Green I and specific primers for 2M), the expected amplification signal is obtained at a Cp-value less than 24.
Absence of contaminating genomic DNA is examined by PCR on a LightCycler® 2.0 Instrument without a reverse transcriptase step; no amplification product is obtained.
Preparation Note
To prepare tissue sections for RNA isolation, fixation reagents must be removed from the samples; after deparaffinization, the sections are ready to be processed with the High Pure FFPE RNA Micro Kit. Deparaffinized tissue samples are disrupted and homogenized during incubation with Proteinase K and a chaotropic salt (guanidine HCl). The homogenate is then applied to the glass fiber fleece in a High Pure Micro Filter Tube.
Under the buffer conditions used in the procedure, all nucleic acids bind specifically to the glass fiber fleece, while contaminating substances (salts, proteins, and other tissue contaminants) do not. DNA in the preparation is digested with DNase I directly on the filter. Brief wash-and-spin steps readily remove the digested DNA fragments and other contaminating substances. The remaining purified RNA is then eluted in a small volume of low-salt buffer.
Analysis Note
Typical RNA Recovery
Starting Material and Quantity: 1 - 10 µm FFPE sections, colon, breast, liver, kidney, spleen of mammalian species
Yield/Recovery: 1.5 - 3.5 µg/5 µm section
Time Required: 60 minutes without 3 hour incubation
Number of Reactions: 50/1-10 µm sections

High Pure Technology and Silica Adsorption Kits,
For general laboratory use.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 50 isolations

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H312 + H332 - H314 - H317 - H334 - H335 - H412
pcodes	P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

High Pure miRNA Isolation Kit 5080576001

In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.
Low to medium throughput miRNA isolation.
The High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin-embedded sections) or plant material. It can also be used to purify total RNA or to prepare samples enriched for small RNAs (<100 nucleotides).
MicroRNAs are natural regulators of gene expression, affecting almost every cellular process (growth and development, cell differentiation, cell death). They occur naturally in diverse animal and plant species, and hundreds have been discovered since the late 1990s. A single miRNA can ramp down expression of multiple genes. Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Capacity: The High Pure Spin Filter Tubes hold up to 700 µL sample volume.

The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:

Northern blotting
cDNA synthesis
Primer extension
miRNA array hybridization
Relative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System

Quality
More than 60% recovery is obtained when 1 µg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.

Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription. ideal for qualitative and quantitative reverse transcription.
Obtain excellent performance and linearity in RT-PCR.
Generate stable , highly pure miRNAs using a simple, efficient protocol.
Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers miRNA purification kits.
Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.

Components

Tissue Lysis Buffer (for FFPE sections)
Proteinase K, recombinant (for FFPE sections)
Binding Buffer
Binding Enhancer
Wash Buffer
Elution Buffer
High Pure Filter Tubes
Collection Tubes

High Pure Technology and Silica Adsorption Kits,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 50 isolations

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H312 + H332 - H314 - H317 - H334 - H335 - H360Df - H412
pcodes	P201 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P308 + P313
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	285.8 °F
Flash Point C	141 °C

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. The amount of DNA recovered is dependent on the amount of DNA applied to the glass fiber fleece, the elution volume, and the length of the amplification/DNA products. When 5 - 25 µg DNA is applied to the kits High Pure Micro Filter Tube, approximately 85% of the DNA can be recovered.
Capacity of High Pure Micro Filter Tubes: The High Pure Micro Filter Tubes hold up to 500 µL sample volume.
The High Pure PCR Cleanup Micro Kit efficiently purifies products from PCR and other reactions. The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions such as labeling, sequencing, or cloning of PCR products.

High Pure PCR Cleanup Micro Kit

The High Pure PCR Cleanup Micro Kit is used to isolate PCR products from amplification and other reactions, and can be used in many molecular biology applications:

Restriction enzyme digests
Alkaline phosphatase treatment
Kinase reactions
Nonradioactive labeling

The kit can also be used to:

Purify cDNA
Concentrate dilute nucleic acid solutions
Recover DNA from agarose gel slices

Use one kit for a variety of applications.
The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 µL (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR. The purified DNA can also be used for Southern blotting and in vitro transcription.
Features and Benefits

Conserve resources and save time

by using a single kit with a simple, rapid protocol.

Obtain purified product in a small elution volume

(10 µL) for demanding downstream applications.

Generate contaminant-free DNA

for direct use in cloning, ligation, restriction digests, and other reactions.

Selectively isolate specific DNA fragment sizes

by using the kits Binding Enhancer to adjust purification stringency.

Eliminate the use of hazardous organic compounds

such as cesium chloride, phenol, chloroform, and ethidium bromide
Components

Binding Buffer
Binding Enhancer
Wash Buffer Concentrate
Elution Buffer
High Pure Micro Filter Tubes
Collection Tubes

Quality
Greater than 70% recovery is obtained when 3 µg Roche DNA Molecular Weight Marker VI is applied to the kits High Pure Micro Filter Tubes. Gel electrophoresis of the eluate after purification confirms the complete removal of primer-dimers. The eluted, purified DNA shows no inhibition of amplification using a LightCycler® Instrument with the LightCycler® FastStart DNA MasterPLUS SYBR Green I.
Preparation Note
Nucleic acids bind specifically to the surface of glass fibers in the presence of chaotropic salts. Since the binding process is specific for nucleic acid, the bound material can be separated and purified from impurities by a simple wash step. The Binding Enhancer enables the modification of DNA fragment size exclusions. Small oligonucleotides and dimerized primers from amplification reactions are selectively removed. The nucleic acids elute from the glass fiber fleece in a low-salt buffer or water.
Analysis Note
A 341 bp PCR fragment of the tPA gene was amplified according to a standard block cycler protocol. The resulting reaction mixes were pooled and purified with the High Pure PCR Cleanup Micro Kit. Different amounts of Binding Enhancer were used in the purification procedure. Portions of the PCR product (250 ng each, lanes 2 – 5) and the PCR product mix (16 µL each, lanes 6 – 7) were analyzed on a 1% agarose gel (see Figure 1).
The yield from each purification is shown in Table 1.

High Pure Technology and Silica Adsorption Kits,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 200 purifications (04983912001), pkg of 50 purifications (04983955001)

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H332 - H314 - H360Df - H412
pcodes	P201 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P308 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	285.8 °F
Flash Point C	141 °C

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Capacity: The High Pure SpinFilter Tubes hold up to 700 µl sample volume.
Volume: 100 µl
Typical samples include:

High Pure PCR Product Purification Kit

Products from amplification or cDNA synthesis reactions
Enzymatically treated DNA
DNA from agarose slices
Dilute nucleic acid solutions
RNA from transcription reactions

The High Pure PCR Product Purification Kit efficiently and conveniently isolates PCR products from amplification reactions and purifies nucleic acids from other modification reactions. It is also recommended for the purification of cDNA.

The High Pure PCR Product Purification Kit is designed for the preparation of concentrated, purified DNA, and can be used directly for most molecular biology applications:

Labeling
Sequencing
Cloning
Restriction enzyme digests
Alkaline phosphatase treatment
Kinase reactions

The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications.
The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 µl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR.
Features and Benefits

Quickly purifies multiple PCR products

in <10 minutes, and purify DNA from agarose gel slices in <20 minutes.

Efficiently recover DNA fragments

>100 bp in length, and concentrate dilute nucleic acid solutions.

Minimize DNA loss

with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.

Eliminate the use of hazardous organic compounds

such as cesium chloride, phenol, chloroform, and ethidium bromide
Components

Binding Buffer
Wash Buffer
Elution Buffer
High Pure Spin Filter Tubes (containing glass fiber fleece)
Collection Tubes

Quality
More than 70% recovery is obtained when 10 μg DNA Molecular Weight Marker VIII mixed with 16 μg bovine serum albumin are applied to the High Pure Filter Tubes. Gel electrophoresis of the DNA eluate confirms the complete removal of protein and DNA fragments smaller than 100 bp.
Preparation Note
The sample is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, all nucleic acids (NA) in the sample bind to the glass fleece in the High Pure tube, while contaminating substances (salts, proteins, nucleotides, mineral oil and other contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the NA can be easily eluted in a small volume of low salt buffer.

High Pure Technology and Silica Adsorption Kits,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 250 purifications (11732676001), pkg of 50 purifications (11732668001)

pictograms	GHS05,GHS07
signalword	Danger
hcodes	H302 + H332 - H314 - H412
pcodes	P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

High Pure PCR Template Preparation Kit 11796828001

Low to medium throughput genomic DNA isolation.
High Pure PCR Template Preparation Kit; Instructions For Use,
Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Note: A special Inhibitor Removal Buffer is included which allows the use of heparinized sample material (100 U/mL of heparin). This buffer increases the sensitivity and reproducibility of assays performed with the isolated nucleic acid, even when the sample contains heparin.
Capacity: The High Pure Spin Filter Tubes hold up to 700 µL sample volume.

High Pure PCR Template Preparation Kit has been used in the extraction of genomic DNA from whole blood samples, cervical lesion specimens, gastric muscosal tissue and cervical carcinoma cell lines.
The High Pure PCR Template Preparation Kit purifies nucleic acids from a wide variety of sample materials including whole blood, cultured cells, and tissue. The isolated nucleic acids can be used for:

Long-template PCR
Real-time, quantitative PCR
SNP detection
Southern blotting
Cloning

Features and Benefits
The High Pure PCR Template Preparation Kit purifies nucleic acids from a wide variety of sample materials, including whole blood, cultured cells, and tissue samples. Bacteria and yeast require a specific pre-lysis treatment with lysozyme or lyticase.

Minimize DNA loss using a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
Recover high molecular weight DNA (30 to 50 kb) that is suitable for long-template PCR.
Improve reliability and reproducibility in downstream applications (real-time, quantitative PCR).
Save time and maximize flexibility by preparing multiple PCR templates simultaneously.
Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

Components

Tissue Lysis Buffer
Binding Buffer
Proteinase K, recombinant PCR grade
Inhibitor Removal Buffer
Washing Buffer
Elution Buffer
High Pure Filter Tubes
Collection Tubes

Quality
DNA is isolated from 25 mg of calf thymus, 1 x 106 K562 cells, and 200 µl of EDTA whole blood. Yield is measured via OD for tissue and cell samples. The quality of the nucleic acid is controlled in an Expand Long Range PCR with a 9.3 kb amplification product for DNA derived from cells. PCR on a LightCycler® Instrument is performed on human blood research samples using kits for Factor V Leiden and CycA.
Principle
Blood cells or tissue are lysed by a short incubation with a special Lysis Buffer and Proteinase K in the presence of a chaotropic salt such as guanidine-HCl, which immediately inactivates all nucleases. Cellular nucleic acids bind selectively to special glass fibers pre-packed in the High Pure Purification Filter Tube. Bound nucleic acid is purified in a series of rapid wash-and-spin steps to remove contaminating cellular components. Finally, low salt elution releases the nucleic acid from the glass fiber. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 100 purifications

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 + H332 - H315 - H317 - H318 - H334 - H335 - H412
pcodes	P261 - P264 - P280 - P304 + P340 + P312 - P305 + P351 + P338 + P310 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Low to medium throughput, mini scale, plasmid isolation.
The High Pure Plasmid Isolation Kit isolates purified plasmid DNA in small quantities using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination.
Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Capacity: The High Pure Spin Filter Tubes hold up to 700 µL sample volume.
Sample: 0.5 - 4.0 mL cultures of E. coli (harvested at a density of 1.5 - 5.0 A 600 units/mL)

High Pure Plasmid Isolation Kit

The High Pure Plasmid Isolation Kit prepares up to 15 µg purified plasmid DNA from bacterial cultures, that can be used directly in most molecular biology applications:

PCR
Sequencing
Cloning
In vitro transcription
Restriction enzyme digests
Random primed labeling

Quality
Plasmid pUC19 (4.5 µg) is purified from a 1.5 mL suspension of E. coli JM83, which was grown in LB-medium with ampicillin for 16 hours, to a cell density of 5 A 600 units/mL. 1 µg of the purified plasmid DNA is incubated for 1 hour at +37°C with 5 units of the restriction endonuclease Eco RI and then analyzed by agarose gel electrophoresis. The isolated plasmid DNA is as sensitive to restriction endonuclease digestion as plasmid DNA isolated by CsCl density centrifugation.
Preparation Note
The kit relies on alkaline lysis to release plasmid DNA from bacteria. RNase removes all RNA in the lysate. After cellular debris and (entrapped) genomic DNA are removed by centrifugation, the remaining supernatant is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, the plasmid binds to the glass fiber fleece, while contaminating substances (salts, proteins, and other cellular contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the plasmid can be easily eluted in a small volume of low-salt buffer or water.

Quickly purify up to 24 plasmid samples in <30 minutes.
Minimize DNA loss with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
Improve reliability and reproducibility in downstream applications with a kit that removes RNA and other impurities that cause plasmid DNA to behave unpredictably.
Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.

Components

Suspension Buffer
RNase A, dry powder
Lysis Buffer
Binding Buffer
Wash Buffer I
Wash Buffer II
Elution Buffer
High Pure Spin Filter Tubes (containing glass fiber fleece)
Collection Tubes

High Pure Technology and Silica Adsorption Kits,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 250 purifications (11754785001), pkg of 50 purifications (11754777001)

pictograms	GHS05,GHS07
signalword	Danger
hcodes	H302 + H332 - H314
pcodes	P261 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310
RIDADR	UN 1824 8 / PGIII
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Histone 10223565001

Mixture of histones H1, H2A, H2B, H3, and H4, isolated from calf thymus.
Histones are a group of DNA-binding proteins that are characterized by relatively high levels of lysine and arginine. Five different fractions of histones have been isolated and characterized. These are named as H1, H2A, H2B, H3 and H4. The H1 fraction is lysine-rich, while the H2A and H2B are slightly lysine-rich, The H3 and H4 fractions are arginine-rich. The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction.

Histone from calf thymus has been used:

in in vitro kinase assay to check the phosphorylation of histones by protein kinase A.
to check the in vitro methyltransferase activity of protein argininemethyltransferases from Oryza sativa (OsPRMTs).
in anti-histone ELISA.

Biochem/physiol Actions
Histones have an important role in the organization and modification of chromatin. The nucleosome is the basic unit of the chromatin. It is made up of two molecules each of histones H2A, H2B, H3 and H4, wrapped with approximately 146bp of DNA. Histone H1 binds to the nucleosomes at the DNA entry and exit points and stabilizes them.
Physical form
Lyophilizate (natural mixture of histones H1, H2A, H2B, H3, and H4).
Preparation Note
Working concentration: 1 to 5 mg/ml.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): Aqueous solution is stable at -15 to -25 °C for several months.
Avoid repeated freezing and thawing!

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 10 mg
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Histone H3 11034758001

Lyophilizate.
Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). Histone H3 is located on human chromosome 6p22. H3.1 is also called as HIST1H3E. It is mainly expressed in the S phase.

Histone H3 has been used to determine the role of histones in metastasis. It has also been used in dot blot and western blotting.
Biochem/physiol Actions
Histone proteins are basic building blocks of chromatin. Mutations in Histone H3 results in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that “drive” chromatin condensation. H3.1 acts as a replication-dependent histone.
Quality
Typical analysis: The preparation is electrophoretically homogeneous.
Preparation Note
Working solution: Solvent is recommended in double-distilled water.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 1 mg
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable

Human Genomic DNA 11691112001

DNA is composed of units called nucleotides arranged into two long polynucleotide chains, resulting in a double helical structure. Nucleotides contain a phosphate group, a sugar group and a nitrogen base. There are four types of nitrogen bases namely adenine (A), thymine (T), guanine (G) and cytosine (C). The arrangement of these bases establishes the genetic codon. The difference in the nucleotide sequences is the reason why organisms differ from one another. In eukaryotic cell, the DNA is localized to the nucleus.
High molecular weight (>50kb) genomic DNA isolated from human blood (buffy coat) by the method of Sambrook et al..

Human Genomic DNA is suitable for:

Southern hybridization analysis
genomic library construction
the amplification of large DNA targets by the Expand System
to assess the quality or integrity of DNA sample using qPCR or real time (RT) PCR and as a control during DNA sequencing

The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.
Biochem/physiol Actions
DNA is an essential component of the mechanism for heredity. The nucleotide sequences carry information regarding different biological processes. The genetic codons encode proteins essential for biological function. This genetic information is transmitted to the next generation during cell division. The amplification of very long fragments of genomic DNA requires template DNA of very high quality. In some cases, amplification failure may be due to poor template quality. This particular quality of genomic DNA is prepared to ensure reliable amplification of long DNA fragments.
Quality
Molecular weight: The preparation is electrophoretically separated on a 0.5% agarose gel and the gel is stained with ethidium bromide. The molecular weight of the purified genomic DNA is greater than 50kb.
Function test: The preparation is used as template in a PCR with the Expand PCR System and appropriate primers from the human tPA Control Primer Set. Amplification products up to 27kb long are obtained.
Absence of contaminating organisms: The serum used for this preparation was tested for HBs antigen and the presence of antibodies to HIV-1, HIV-2, HCV. All tests were negative.
Physical form
Solution in 10 mM Tris HCl, 1 mM EDTA, pH 8.0

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 100 μg
Торговая марка	Roche
concentration	0.2 mg/mL
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
Flash Point F	No data available
Flash Point C	No data available

Hybridoma Fusion and Cloning Supplement 11363735001

Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).
HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).

Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
Physical form
Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.
Analysis Note
Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
sterility	0.2 μm filtered
form	solution
packaging	pkg of 10 mL (50x)
Торговая марка	Roche
application(s)	cell culture \| hybridoma: suitable
impurities	Mycoplasma, tested, 10 EU/mL Endotoxin (LAL)
solubility	water: miscible
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Hygromycin B 10843555001

Empirical Formula (Hill Notation):	C₂₀H₃₇N₃O₁₃
CAS Number:	31282-04-9
Molecular Weight:	527.52
MDL number:	MFCD06795479
PubChem Substance ID:	329749191

Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
Biochem/physiol Actions
Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.
Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Quality
Purity: >80% (HPLC)
Unit Definition
Unit Assay: Unit/mg
We do not have this information.
Physical form
Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.
Storage and Stability
Store at 2 to 8 °C. (solution)

For life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.

sterility	non-sterile; 0.2 µm filtered
assay	80% (HPLC)
form	buffered aqueous solution
composition	Hygromycin B, >80% HPLC
packaging	pkg of 20 mL (1 g)
Торговая марка	Roche
concentration	50 mg/mL
application(s)	transfection: suitable
shipped in	wet ice
SMILES string	CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
InChI	1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key	GRRNUXAQVGOGFE-XKIAHZFYSA-N

pictograms	GHS05,GHS06,GHS08
signalword	Danger
hcodes	H301 - H312 - H317 - H318 - H330 - H334
pcodes	P260 - P280 - P301 + P310 + P330 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P342 + P311 - P403 + P233
RIDADR	UN 2810 6.1 / PGIII
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Immunoprecipitation Kit (Protein A) 11719394001

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.
Immunoprecipitation is a widely used method for the analysis of target antigens in complex mixture of proteins. The protein of interest can be concentrated and immunoaffinity -purified in one step on an analytical scale via a specific antibody. Often, immunoprecipitated proteins are functionally fully active and can be further analyzed with respect to enzymatic activity, interactions, modifications and structure.

Immunoprecipitation Kit (Protein A) has been used for the immunoprecipitation of proteins from cellular extracts with Protein A Agarose.
Packaging
1 kit containing 5 components.
Preparation Note
Working solution: Lysis buffer/wash buffer 1
The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2
The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 20 reactions
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS09
signalword	Danger
hcodes	H314 - H317 - H411
pcodes	P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

Immunoprecipitation Kit (Protein G) 11719386001

Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins.
Specificity
Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.

The immunoprecipitation kit has been used for the immunoprecipitation of proteins from cellular extracts with Protein G Agarose.
Packaging
1 kit containing 5 components.
Preparation Note
Working solution: Lysis buffer/wash buffer 1

The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations.
To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete Tablet. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing.
Wash buffer 2

The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation.
To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.
Wash buffer 3
The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation.
To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml.
Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 20 reactions
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS09
signalword	Danger
hcodes	H314 - H317 - H411
pcodes	P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

In Situ Cell Death Detection Kit, AP 11684809910

Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunohistocytochemistry.
Contents:

Enzyme Solution (TdT)
Label Solution (fluorescein-dUTP)
Converter AP (anti-fluorescein antibody-AP), ready-to-use

Specificity
The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. This allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation.

The In Situ Cell Death Detection Kit, AP, is a precise, fast, and simple nonradioactive technique to detect and quantify apoptotic cell death at the single-cell level in cells and tissues by light microscopy; it is not intended for diagnostic procedures. Thus, the In Situ Cell Death Detection Kit can be used in many different assay systems. Examples are:

Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research†
Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research
Typing of cells undergoing cell death in heterogeneous populations by double staining procedures

Packaging
1 kit containing 3 components.
Specifications
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2-deoxy-uridine. The methods involve the separation of fragmented, low molecular-weight DNA from unfragmented, high molecular-weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population or, particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Principle
The In Situ Cell Death Detection Kit, AP is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and fluorescein-dUTP. During this incubation period, TdT catalyzes the addition of fluorescein-dUTP at free 3-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is marked by an anti-fluorescein antibody conjugated with the reporter enzyme alkaline phosphatase. After washing to remove unbound enzyme conjugate, the AP retained in the immune complex is visualized by a substrate reaction.
Preparation Note
Working solution: Add total volume (50 µl) of Enzyme Solution to the remaining 450 µl Label Solution to obtain 500 µl TUNEL reaction mixture.
Mix well to equilibrate components.
Storage conditions (working solution): TUNEL reaction mixture
The TUNEL reaction mixture should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Converter-AP
Once thawed the Converter-AP solution should be stored at 2 to 8 °C (maximum stability
6 months).
Note: Do not freeze.

Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method
Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary detection system
Convenient: The direct labeling procedure using fluorescein-dUTP allows verification of the efficiency of the TUNEL reaction during the assay procedure
Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis
Flexible: No substrate included; provides the opportunity to select the staining procedure of choice

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 50 tests
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07,GHS08,GHS09
signalword	Danger
hcodes	H317 - H350i - H411
pcodes	P201 - P261 - P273 - P280 - P308 + P313 - P391
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

In Situ Cell Death Detection Kit, Fluorescein 11684795910

Kit for the detection and quantification of apoptosis at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology); analysis by fluorescence microscopy or flow cytometry.
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2-deoxy-uridine. The methods involve the separation of fragmented, low molecular weight DNA from unfragmented, high molecular weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population, or particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Specificity
The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. This allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation.

The In Situ Cell Death Detection Kit, Fluorescein, is a precise, fast, and simple nonradioactive technique to detect and quantify apoptotic cell death at the single-cell level by fluorescence microscopy and quantitative detection by flow cytometry in cells and tissues. Thus, the In Situ Cell Death Detection Kit can be used in many different assay systems.
Examples are:

Detection of individual apoptotic cells in frozen and formalin-fixed tissue sections in basic research
Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research
Typing of cells undergoing cell death in heterogeneous populations by double staining procedures

Packaging
1 kit containing 2 components.
Preparation Note
Working solution: Add total volume (50 µl) of Enzyme Solution to the remaining 450 µl Label Solution to obtain 500 µl TUNEL reaction mixture.
Mix well to equilibrate components.

Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling
Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction
Convenient: No secondary detection system required
Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 50 tests
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

pictograms	GHS08,GHS09
signalword	Danger
hcodes	H350i - H411
pcodes	P201 - P273 - P280 - P308 + P313 - P391 - P501
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

In Situ Cell Death Detection Kit, POD 11684817910

Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunohistocytochemistry.

The In Situ Cell Death Detection Kit, POD, is a precise, fast, and simple nonradioactive technique to detect and quantify apoptotic cell death at the single-cell level in cells and tissues. Thus, the In Situ Cell Death Detection Kit can be used in many different assay systems.

Examples are: Detection of individual apoptotic cells in frozen, paraffin-embedded and formalin-fixed tissue sections in basic research and routine pathology
Determination of sensitivity of malignant cells to drug-induced apoptosis in cancer research and clinical oncology
Typing of cells undergoing cell death in heterogeneous populations by double staining procedures

Features and Benefits
Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method.
Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary detection system.
Convenient: The direct labeling procedure using fluorescein-dUTP allows verification of the efficiency of the TUNEL reaction during the assay procedure.
Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis.
Flexible: No substrate included; provides the opportunity to select the staining procedure of choice.
Packaging
1 kit containing 3 components.
Principle
The In Situ Cell Death Detection Kit, POD is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and fluorescein-dUTP. During this incubation period, TdT catalyzes the addition of fluorescein-dUTP at free 3-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is marked by an anti-fluorescein antibody conjugated with the reporter enzyme peroxidase. After washing to remove unbound enzyme conjugate, the POD retained in the immune complex is visualized by a substrate reaction.
Preparation Note
Storage conditions (working solution): TUNEL reaction mixture
The TUNEL reaction mixture should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Converter-peroxidase
Once thawed the Converter-peroxidase solution should be stored at 2 to 8 °C (maximum stability 6 months).
Note: Do not freeze.
Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 50 tests
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07,GHS08,GHS09
signalword	Danger
hcodes	H317 - H350i - H411
pcodes	P201 - P261 - P273 - P280 - P308 + P313 - P391
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

In Situ Cell Death Detection Kit, TMR red 12156792910

Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2-deoxy-uridine. The methods involve the separation of fragmented, low molecular weight DNA from unfragmented, high molecular weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population, or particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages, is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks (nicks). Both types of breaks can be detected by labeling the free 3-OH termini with modified nucleotides (e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP) in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes the template-independent polymerization of deoxyribonucleotides to the 3-end of single- and double-stranded DNA. This method has also been termed TUNEL (TdT-mediated dUTP-X nick end labeling). Alternatively, free 3-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Sample material: Cells in suspension, cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Principle
The In Situ Cell Death Detection Kit, TMR red is based on the detection of single- and double-stranded DNA breaks that occur at the early stages of apoptosis.
Apoptotic cells are fixed and permeabilized. Subsequently, the cells are incubated with the TUNEL reaction mixture that contains TdT and TMR-dUTP. During this incubation period, TdT catalyzes the addition of TMR-dUTP at free 3-OH groups in single- and double-stranded DNA. After washing, the label incorporated at the damaged sites of the DNA is visualized by flow cytometry and/or fluorescence microscopy.

Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red).
Specificity
The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. This allows discrimination of apoptosis from necrosis and from primary DNA strand breaks induced by cytostatic drugs or irradiation.

In Situ cell death detection kit, TMR red has been used in terminal deoxynucleotidyl transferase biotin–dUTP nick end labeling (TUNEL) assay. It has been used in apoptosis detection assay.
Precise, fast, and simple technique for detecting and quantitating apoptotic DNA fragmentation at the single-cell level in cells and tissues with a red fluorescent label for fluorescence microscopy and flow cytometry.
Packaging
1 kit containing 2 components.
Preparation Note
The TUNEL reaction mixture is prepared by mixing the Enzyme Solution and the Label Solution prior to use.
Working concentration: Enzyme concentration
The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 µl PCR, we recommend using 2 U of the enzyme blend.
Working solution: Add total volume (50 µl) of Enzyme Solution to the remaining 450 µl Label Solution to obtain 500 µl TUNEL reaction mixture.
Mix well to equilibrate components.
Storage conditions (working solution): The TUNEL reaction mixture should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 50 tests
Quality Level	100,
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

pictograms	GHS08,GHS09
signalword	Danger
hcodes	H350i - H411
pcodes	P201 - P273 - P280 - P308 + P313 - P391 - P501
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

Insulin, human 11376497001

Empirical Formula (Hill Notation):	C₂₅₇H₃₈₃N₆₅O₇₇S₆
CAS Number:	11061-68-0
Molecular Weight:	5807.57
MDL number:	MFCD00131380

Recombinant Insulin, human, is produced in yeast and purified by standard chromatographic techniques. Insulin regulates blood glucose. In muscles and adipocytes it stimulates glucose intake and metabolism. It also controls the expression and various enzymes.
Specificity
Insulin, human, is active on most mammalian cells.

Insulin shows a broad range of activities on a variety of somatic cells. Recombinant human insulin can be used to stimulate growth and proliferation of cultured cells and to investigate insulin activity on sensitive cells used in research studies. It is also a component of serum-free media formulations for most primary cells and cell lines.
Biochem/physiol Actions
Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
For life science research only. Not for use in diagnostic procedures.
Quality
Endotoxin level: <0.1EU/µg (LAL-test)
Note: 1 EU corresponds to 0.1ng
Sequence
A-chain: 21 AS, B-chain: 30 AS, connected by two disulfide bridges
Two polypeptide chains (A-chain: 21 amino acids, B-chain: 30 amino acids, connected by two disulfide bridges) identical to natural, human insulin.
Unit Definition
EC50 definition: The concentration of human insulin that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 3T3 (A31) cells.
Physical form
Lyophilizate from a hydrochloric acid solution (pH 2.3) (crystalline insulin)
Preparation Note
Working concentration: 1-10 µg/ml
Recommended concentration for serum-free cell culture is 1-10 µg/ml.
The concentration of insulin required for stimulation of cell growth in almost all cases is extraordinarily high compared with the physiological concentration. Insulin may be mimicking insulin-like growth factors (IGFs, somatomedins) for some cell lines, and high insulin concentrations may be necessary to occupy receptors which have a high affinity for IGFs and a lower affinity for insulin.
Working solution: Dissolve Insulin, human, recombinant (100 mg or 500 mg), in sterile, double-dist. water (final concentration: 10 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The reconstituted, undiluted solution is stable at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Reconstitute in sterile double-distilled water (final concentration 10 mg/ml), further dilution with PBS (phosphate buffered saline) or medium containing 1 mg/ml (0.1%) BSA (bovine serum albumin) [or HSA (human serum albumin)], or 1 to 10% serum.

Quality Level	100
sterility	non-sterile; 0.2 µm filtered
assay	>98% (SDS-PAGE)
form	lyophilized (clear, colorless solution after reconstitution)
specific activity	>26 units/mg protein (At least the same specific activity (EC<sub>50</sub>) compared to the indicated standard is guaranteed.)
mol wt	5,700 Da
packaging	pkg of 100 mg
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
impurities	<0.1 EU/µg endotoxin (LAL test)
solubility	water: soluble
UniProt accession no.	P01308,
shipped in	wet ice
storage temp.	2-8°C
InChI key	PBGKTOXHQIOBKM-FHFVDXKLSA-N
Gene Information	human ... INS(3630)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable

Insulin-Transferrin-Sodium Selenite Supplement 11074547001

The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.
Preparation Note
Working concentration: Insulin, 5 µg/ml, transferrin, 5 µg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M)
Storage conditions (working solution): -15 to -25 °C.
Prepare appropriate aliquots and avoid repeated freezing and thawing.
Reconstitution
The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human serum (transferrin)","synthetic (sodium selenite)
recombinant	expressed in human cells
sterility	non-sterile; 0.2 µm filtered
assay	>98% (Transferrin, SDS-PAGE)",">99% (Insulin, HPLC)",">99% (Sodium selenite)
form	lyophilized
packaging	pkg of 50 mg (for 5 l medium)
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
solubility	water: miscible
UniProt accession no.	P01317,","P02786,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	bovine ... INS(280829) human ... TFRC(7037)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Interferon-, human (hIFN-) 11040596001

IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens. Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers.
Specificity
Human IFN-γ is effective on human cells, but not on mouse or rat cells.

Interferon-γ, human (hIFN-γ) has been used to stimulate mesenchymal stem cells (MSCs). It has also been used as a supplement Dulbecco′s modified Eagle′s medium/Ham′s medium for the cultivation of human colorectal carcinoma cell line T84 cells.
Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems.
Biochem/physiol Actions
Interferon-γ (IFN-γ) is an essential component of acquired immunity. A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.
A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γs primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.
Quality
The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative.
Endotoxin level: <0.1EU/µg (LAL-test), <10EU/ml (LAL-test)
Note: 1EU corresponds to 0.1ng
Sequence
Chain Length 143 AA
The primary structure of recombinant human IFN-γ (143 amino acids) is identical to that of natural human IFN-γ (146 amino acids), however, recombinant IFN-γ has three amino acids less and is not glycosylated. Glycosylation is not essential for biological activity.
Unit Definition
EC50 definition: The amount of hIFN- that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN- reference standard (Gg 23– 901-530) (WISH cells-EMC virus/cytopathic effect) (1 unit equals 0.05 ng/ml).
Physical form
100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane.
Preparation Note
Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium.

For life science research only. Not for use in diagnostic procedures.
EC 50 : <0.05ng/ml (hIFN- , NIH, reference standard, Gg 23–901-530)

Quality Level	100
biological source	human
recombinant	expressed in E. coli
assay	95% (SDS-PAGE)
form	solution
potency	<0.05 ng/mL EC₅₀
specific activity	>2 x 10^7 U/mg
mol wt	16,700 Da
packaging	pkg of 100,000 U (5 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<0.1 EU/µgtested (LAL test)
UniProt accession no.	P01579,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... IFNG(3458)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Interferon-, mouse (mIFN-) 11276905001

IFN-γ (interferon-γ) is produced by natural killer T cells, macrophages, Th1 CD4 T cells and activated CD8 T cells and the production increases with age.
Recombinant, Interferon-, mouse (mlFN-) is produced in E. coli and purified by standard chromatographic techniques.
Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity.
Specificity
Species specificity: mouse

Interferon-γ, mouse (mIFN-γ) has been used in cell culture.
Recombinant mouse interferon-γ is a valuable tool for the study of IFN-γ (interferon-γ) actions in mouse model systems.
Biochem/physiol Actions
Interferon-γ (IFNγ) participates in cell-mediated immunity and has pro-inflammatory effects on immunocytes and target tissue. Its functions vary and is tissue specific. IFNγ induces T-cell-mediated immune response by increasing MHC-I expression on target cells and acts through IFNγ receptor, which is expressed on both immune and non-immune cells. IFNγ is associated with the development of colitis and Sjogren Syndrome.
Quality

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test)

Sequence
The primary structure of recombinant Interferon-γ, mouse (mIFN-γ) is identical to that of natural, mouse IFN-γ (one polypeptide chain, 131 amino acids), however, recombinant mIFN-γ lacks the last two amino acids at the C-terminus and is not glycosylated. Glycosylation is not essential for biological activity.
Chain Length 131 AA
Unit Definition
EC50 definition: One unit is defined as the amount of mlFN- that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN- reference standard (Gg 02-901-533). (L cells-EMC virus/cytopathic effect) (1 unit equals 0.2 ng/ml).
EC 50 definition : The amount of mIFN- that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN- reference standard (Gg 02–901-533) (L cells-EMC virus/cytopathic effect) (1 unit equals =0.2 ng/ml).
Physical form
Solution, filtered through 0.2 μm pore size membrane, 100 000 U/ml (20 μg/ml) in PBS (phosphate buffered saline) and BSA (bovine serum albumin),1 mg/ml [purity of BSA: 98%, endotoxin (LAL): < 1 EU/mg BSA].
Preparation Note
Working solution: Dilute the concentrated mlFN- solution (100,000 U/ml) with PBS or culture medium containing BSA, 1 mg/ml (0.1%) or serum, 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

For life science research only. Not for use in diagnostic procedures.
For life science research only. Not for use in diagnostic procedures.
1 EU corresponds to 0.1 ng.

Quality Level	100,
biological source	mouse
assay	>95% (SDS-PAGE)
form	solution
specific activity	>5*10^6 U/mg ((mIFN- , NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.)
packaging	pkg of 100,000 U (20 µg, 1 ml)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

Recombinant Interleukin-2, human (hIL-2) is produced in E. coli and purified by standard chromatographic techniques. Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 µm pore size membrane.
Specificity
Species specificity: Recombinant IL-2, human is effective on mouse and human cells.

Interleukin-2, human (hIL-2)

Recombinant IL-2, human, allows:

The cultivation of human and murine IL-2 dependent T-cell lines and natural killer cell lines.
The proliferation of mitogen-activated T-lymphocytes and natural killer cells.
The establishment of human and murine thymocyte, splenocyte, or peripheral blood lymphocyte (PBL) derived T-cell lines.
The generation of human and murine lymphokine-activated killer (LAK) cells (1-11).
In vitro re-stimulation of lung cells obtained from mice.

Biochem/physiol Actions
Interlekin-2 (IL-2) is a potent pro-inflammatory cytokine. It plays a protective role in autoimmune chronic inflammation, when present in low amounts. It is involved in maintaining immune homeostasis where it controls the cross-talk between T regulatory and T effector cells. Studies in acute myelogenous leukemia (AML) mice models show that antibody based delivery of IL-2 to targets present in tumor-linked vasculature results in antileukemic activity.
Quality
Purity: >95% pure as determined by SDS-PAGE
Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test)
(1 EU corresponds to 0.1 ng)
Sequence
Chain Length 134 AA
The primary structure of recombinant, IL-2, human is identical to that of natural, human IL-2 (one polypeptide chain, 133 amino acids), however, recombinant IL-2 has an extra methionine at the amino-terminus (one polypeptide chain, 134 amino acids) and is not glycosylated. Glycosylation is not essential for biological activity.
Unit Definition
EC50 definition: The amount of hIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals 0.5 ng).
Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)
Physical form
Solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane
Preparation Note
Working concentration: 10 -20 U/ml
Established IL-2-dependent T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependent cell lines.
Working solution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Specific activity/EC 50 : >2 x 106 U/mg <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50 ) compared to the indicated standard is guaranteed (19, 20). Human, recombinant IL-2 has the same biological activity in vitro as compared to human, natural IL-2 (15, 1–4).
Recommended Method of Dilution: Dilute the concentrated IL-2 solution (200 U/ml or 10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA or HSA (0.1%) or 1–10% serum.
Interleukin-2 (IL-2, also known as T-Cell Growth Factor, TCGF) is a lymphokine which is produced by lectin- or antigen-activated T-cells and plays an important immunoregulatory role. This factor, or lymphokine, was first identified by its ability to promote the long-term in vitro proliferation of activated T cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine- activated killer (LAK) cells (1–11). Recombinant human IL-2 allows the cultivation of human and murine IL-2-dependent T-cell lines and natural killer cell lines, the proliferation of mitogenactivated T-lymphocytes and natural killer cells, the establishment of human and murine thymocyte-, splenocyte-, or peripheral blood lymphocyte (PBL)- derived T-cell lines, and the generation of human and murine lymphokine-activated killer (LAK) cells (1-11). Established IL-2-dependant T-cell lines usually require 10-20 U/ml. Add IL-2 to the freezing medium for IL-2 dependant cell lines.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in E. coli
assay	95% (SDS-PAGE)
form	solution
potency	>2 x 10^6 units/mg EC₅₀
mol wt	(15,000 Da)
packaging	pkg of 10,000 U (10799068001 [5 µg, 50 ml])","pkg of 10,000 U (11011456001 [5 µg, 1 ml])","pkg of 50,000 U (11147528001 [25 µg, 5 ml])
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
concentration	10-20U/ml
impurities	<0.1 EU/µg
UniProt accession no.	P60568,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... IL2(3558)

Interleukin-2, mouse (mIL-2) 11271164001

Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1).
Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods.
Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 µm pore size membrane
Specificity
Mouse IL-2 is effective on mouse cells, but not on human cells.

Recombinant murine IL-2 is produced in E. coli and exhibits the following:

Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells.
Strongly inhibits the binding of recombinant human IL-2 to murine responder cells.
Weakly inhibits the binding to human responder cells.
Shares identical biological and immunological activities with human IL-2.
Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models.
used for the stimulation of murine T-cells

Quality
Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test)
Sequence
One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity.
Chain Length 149 AA
Unit Definition
EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals 0.5 ng).
Preparation Note
Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v).
Storage conditions (working solution): -15 to -25 °C
Note: Avoid repeated freezing and thawing.

For life science research only. Not for use in diagnostic procedures.
Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications.
EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng).

Quality Level	100
biological source	mouse
recombinant	expressed in E. coli
assay	>95% (SDS-PAGE)
form	solution
potency	<0.5 ng/mL EC₅₀
mol wt	17,200 Da
packaging	pkg of 10,000 U (5 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<0.1 EU/µgtested (LAL test)
UniProt accession no.	P04351,
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Il2(16183)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

Interleukin-6, human (hIL-6) 11138600001

Interleukin-6, human (hIL-6), is produced in E. coli and purified by standard chromatographic techniques.
Specificity
Recombinant IL-6, human is effective on mouse and human cells.
IL-6, a 26 kD protein, also known as B-cell stimulatory factor 2 (BSF-2, BCDF), interferon-2 (IFN-2), hybridoma/plasmacytoma growth factor (H(P)GF), and hepatocyte-stimulating factor is produced by many cell types (e.g., T-cells, monocytes, fibroblasts, endothelial cells). IL-6 induces the terminal maturation of activated B-cells into antibody-producing cells (B-cell differentiation activity. The hepatic acute phase response is induced by IL-6 (hepatocyte-stimulating activity). It was shown that IL-6 and IL-3 act synergistically to support the proliferation of hematopoietic stem cells (hemapoietic activity), however, IL-6 does not have antiviral activity, although it has been called IFN-2. High affinity receptors for IL-6 are found on many cell types.

Recombinant IL-6, human, is a growth factor for murine and human B-cells. It can be used to replace feeder cells in the preparation of murine and human hybridomas.
Biochem/physiol Actions
Interleukin-6 participates in several pleiotropic functions in various types of cells through its specific receptor (IL-6-R). It has been suggested that interleukin-6 may participate in the interaction between neuroendocrine and immune system by activating adrenocorticotropic hormone secretion. It has also been reported that IL-6 stimulates the bonding of receptor (IL-6-R) with a non-ligand-binding membrane glycoprotein, gp130.
Quality
Purity: 95% pure as determined by HPLC or SDS-PAGE [Endotoxin level: <0.1 EU/µg (LAL-test), <10 EU/ml (LAL-test).
Note: 1 EU corresponds to 0.1 ng
Sequence
Chain Length 184 AA
The primary structure of recombinant human IL-6 is identical to that of natural human IL-6 (one polypeptide chain, 184 amino acids), however, it contains an altered sequence within the first 15 amino-terminal amino acids.
Unit Definition
EC50 definition: The amount of hIL-6 that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 7TD1 cells (1 unit equals <0.01 ng).
Physical form
Solution, filtered through 0.2 μm pore size membrane.
Preparation Note
Working concentration: 10 to 100 U/ml (0.1 1 ng/ml)
10 to 100 U/ml (0.1 to 1 ng/ml)
A concentration of 100 U/ml (1 ng/ml) is recommended for the preparation of B-cell hybridomas
Working solution: Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum.
Analysis Note
Specific activity/EC50: >1 x 108 U/mg, <0.01 ng/ml (hIL-6, NIBSC, interim standard, 88/514), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in E. coli
assay	>98% (SDS-PAGE)
form	solution
potency	<0.01 ng/mL EC₅₀
mol wt	20,600 Da
packaging	pkg of 200,000 U (2 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<0.1 EU/µgtested (LAL test)
UniProt accession no.	P05231,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... IL6(3569)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

Isopropyl--D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by -galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the -galactosidase coding gene lacZ.

Isopropyl--D-thiogalactoside

Empirical Formula (Hill Notation):	C₉H₁₈O₅S
CAS Number:	367-93-1
Molecular Weight:	238.30
Beilstein/REAXYS Number:	4631
MDL number:	MFCD00063273
PubChem Substance ID:	329815691

Isopropyl--D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of -galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.
IPTG is commonly used in cloning procedures that require induction of -galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the -galactosidase coding gene lacZ.

Non-metabolizable galactose analog.
For life science research only. Not for use in diagnostic procedures.
The amount of labeled DNA depends on:

amount of template DNA
purity of template DNA
conformation of template DNA
average size of labeled fragment

Specifications
Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 109 dpm/µg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.
Preparation Note
Sample Material
10 ng to 3µg template DNA, linearized and at least 100 to 200bp long
Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

mol wt	238.3
packaging	pkg of 1 g (10724815001), pkg of 5 g (11411446001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C
SMILES string	CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
InChI	1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key	BPHPUYQFMNQIOC-NXRLNHOXSA-N

IsoStrip™ Mouse Monoclonal Antibody Isotyping Kit 11493027001

IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies.
Contents

Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies
Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies

Specificity
Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA), and to the light chains.
Specificity for all specific antibodies beside IgML at 1.0 µg/ml: 2
IgM Lambda, at 1.0 µg/ml: 1

The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins).
Features and Benefits

Reliable: Yields comparable results to the standard ELISA assay
Fast: 5 minutes from start to finish
Easy: Only 3 steps
Economical: No equipment or additional reagents required
Highly specific: No crossreactivity with FBS

Packaging
1 kit containing 2 components.
Specifications
Assay time: 5 minutes
Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites
Preparation Note
Working solution: Dilute Sample

Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

For life science research only. Not for use in diagnostic procedures.

IsoStrip is a trademark of Roche

usage	sufficient for 10 tests
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart® DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes, allowing for more uniform multiplexed PCR.
KAPA2G Fast HotStart PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance and does not require specialized PCR consumables or thermocyclers.

KAPA2G Fast Multiplex Mix

KAPA2G Fast Multiplex Mix has been used for:

Typing of transgenic organisms
Amplification of microsatellites
Typing and detection of pathogens
Amplification of multiple DNA fragments for single nucleotide polymorphism (SNP) genotyping
Semi-quantitative PCR
3-plex PCR

Biochem/physiol Actions
DNA fragments generated with KAPA2G Fast DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for sequencing, restriction enzyme digestion and cloning. Like wild-type Taq, KAPA2G Fast has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated with KAPA2G Fast are 3-dA-tailed and may be cloned into TA cloning vectors.
Features and Benefits
Improve sensitivity, specificity, and yields

Uniform representation of all amplicons
Successful multiplex PCR with difficult, GC-rich targets

Increase speed without compromising performance

60% reduction in cycling time
Extension times as low as 15 seconds

Quick Notes:

KAPA2G Fast Multiplex Mix contains the engineered KAPA2G Fast HotStart DNA Polymerase, for fastand efficient multiplex PCR.
The KAPA2G Fast Multiplex Mix contains a buffer optimized for multiplex PCR, with 0.2 mM of each dNTP and 3 mM MgCl2 (at 1X).
Use 0.2 µM of each primer, and 10–100 ng of template DNA per reaction.
Anneal at 60°C for 30 seconds.
Perform extension for 15 sec, and increase for longer amplicons, and/or highly multiplexed reactions.

Quality
Each batch of KAPA2G Fast HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (AgilentProtein 230 Assay). KAPA2G Fast Multiplex PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contaminationlevels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the ReadyMix has been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the users own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

shelf life	≤12 mo.
Quality Level	100,
packaging	kit of 1.25 mL (100 x 25 μL rxn; KK5801), kit of 6.25 mL (500 x 25 μL rxn; KK5802)
Торговая марка	Roche
concentration	2 x
application(s)	PCR: suitable
shipped in	dry ice
storage temp.	−20°C

pictograms	GHS07,GHS08
signalword	Warning
hcodes	H302 - H371
pcodes	P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Klenow Enzyme 10104531001

Klenow enzyme is the large, C-terminal fragment (Mr 76,000) of E.coli DNA polymerase I, which can be obtained by subtilisin treatment of intact DNA polymerase I. It retains the 53 polymerase and the 35 exonuclease activities of intact DNA polymerase I, but lacks the 53 exonuclease activity of the native enzyme. The enzyme catalyzes the addition of mononucleotides from deoxynucleoside-5-triphosphates to the 3-hydroxyl terminus of a primer/template DNA. This property is used to synthesize DNA complementary to single-stranded DNA templates.
Klenow Enzyme is formed by subtilisin treatment of DNA polymerase I.

Klenow Enzyme has been used in blunt end ligation of recyclin-1 (RCY1) gene into pETDUET-GSTHis6-S Tag vector.
Quality
Function test: Sequencing Grade Klenow Enzyme is tested in sequencing reactions according to Sanger. The test is conducted with 1 unit of enzyme and the M13 sequencing system. Sequencing gels are examined by autoradiography. The readable sequence must be 250 bases.
Absence of contaminants: Sequencing Grade Klenow Enzyme is tested on various DNA substrates to ensure the absence of nonspecific endonucleases and exonucleases.
Unit Definition
One unit is the enzyme activity which incorporates 10 nmol of total nucleotides into an acid-precipitable fraction in 30 minutes under the following assay conditions:
Incubation Buffer
130 mM Potassium phosphate, 6.5 mM MgCI2, 33 µM 14C-dTTP, poly[d(A-T)], 0.833 A260/ml; dATP, 33 µM; 1 mM dithioerythritol; bovine serum albumin, 0.032 mg/ml; pH 7.4.
Incubation Procedure
0.05 to 0.25 units of enzyme are incubated for 30 minutes at +37 °C in a total volume of 300 µl incubation buffer.
Volume Activity: 1,000 units/ml, or 5,000 units/ml respectively, according to Richardson (+37 °C, poly [d(A-T)] as primer).
Physical form
Solution of enzyme, 5U/µl, in storage buffer:
50 mM Potassium phosphate, 1 mM dithioerythritol, glycerol, 50% (v/v); pH 7.0 (4 °C)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
specific activity	>5000 units/mg protein
mol wt	M_r 75 kDa
packaging	pkg of 250 U (5 x 10³ U/ml)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

L-Carnitine 11242008001

Enzymatic UV-Test with a linearity in the range of 5.6 - 112 μM L-carnitine in the test solution.

Determination of L-carnitine in seminal plasma, serum, or urine in life science research applications.
Biochem/physiol Actions
L-carnitine is an abundant amino acid present in red meat. Its trimethylamine structure has similarity to that of choline. The major source of this amino acid is by dietary ingestion. In mammals, it is also endogenously produced from lysine. It participates in the transport of fatty acids into the mitochondrial compartment. It produces trimethylamine-N-oxide (TMAO) and promotes atherosclerosis.
Packaging
1 kit containing 6 components
Principle
L-Carnitine is acetylated to acetyl carnitine by acetyl coenzyme A (acetyl CoA) in the presence of the enzyme carnitine acetyl transferase (CAT). The resulting coenzyme A (CoA) is acetylated back to acetyl CoA in the presence of adenosine-5-triphosphate (ATP) and acetate, catalyzed by the enzyme acetyl CoA synthetase (ACS). This results in the formation of adenosine-5-monophosphate (AMP) and inorganic pyrophosphate (PPi). In the presence of ATP, supported by myokinase (MK), AMP forms twice the amount of adenosine-5-diphosphate (ADP). This is converted in the following reaction with phosphoenol pyruvate (PEP) in the presence of pyruvate kinase (PK). The pyruvate formed is reduced to L-lactate by reduced nicotinamide adenine dinucleotide (NADH) in the presence of lactate dehydrogenase (LDH). The amount of NADH consumed during the reaction is equivalent to half the amount of L-carnitine. NADH is measured spectroscopically at 340 nm.
Preparation Note
Storage conditions (working solution): Note:

After reconstitution, the solution 1 is stable for 4 days at 2 to 8 °C.
Bring solution 1 to 15 to 25 °C prior to use.
After reconstitution, the suspension 2 is stable for 3 months at 2 to 8 °C.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ~3 x 10 determinations (test-combination), sufficient for ~3 x 10 tests
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05
signalword	Danger
hcodes	H318 - H412
pcodes	P273 - P280 - P305 + P351 + P338 + P310 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

L-Lactate Dehydrogenase (L-LDH)

1.1.1.27 ( BRENDA | IUBMB )

L-lactate:NAD+ oxidoreductase.
L-lactate dehydrogenase catalyzes the reversible reduction of pyruvate to L-lactate.
Specificity
L()-Lactate dehydrogenase (LDH) is specific for L()-lactate (relative rate = 100). It does not react with D(-)-lactate.LDH will also slowly oxidize glyoxylate (oxoacetate), glycerate and 3-halogen derivatives of L-lactate (rate with each, 1); 3-phosphoglycerate does not react.LDH will reduce pyruvate (2-oxopropanate; relative rate = 100) and other 2-oxoacids (e.g., 2-oxobutyrate), with rates decreasing rapidly with increasing chain length of the acid. Heart LDH reacts with 2-oxobutyrate at a greater rate than does skeletal muscle LDH.LDH will also oxidize 2,4-diketoacids (relative rate = 10). The enzyme is realtively specific for NAD(H) (relative rate = 100); NADP(H) is utilized much less efficiently (relative rate < 1). Representative Kms for (hog) skeletal muscle LDH (pH 7.5) are lactate, 8.3 mM; pyruvate, 0.12 mM; NAD, 0.10 mM; NADH, 0.012 mM.Representative Kms for (pig) heart LDH (pH 7.5) are lactate, 3.3 mM; pyruvate, 0.15 mM; NAD, 0.07 mM;NADH, 0.011 mM. In general, the concentration of pyruvate (reduction reaction) or of L-lactate (oxidation reaction) necessary to obtain a maximal rate with LDH is lower for preparations from heart tissue than for the preparations from skeletal muscle.

Reduction of α-ketoacids to α-hydroxycarboxylic acids, or reverse reaction.
Sequence
LDH is a tetramer (MW = approx. 140,000 D, rabbit muscle or pig heart LDH). In mammals, there are two types of LDH subunits, M and H, with similar molecular weight (subunit MW 36,500 D) but differing amino acid composition.
Therefore, 5 electrophoretically distinguishable LDH isoenzymes, LDH-1 through LDH-5, of differing subunit composition, are found in mammals.
The molecular weight of all LDH isoenzymes is approximately the same.
– LDH-1 (H4), LDH-2 (H3M): predominant components of heart LDH
– LDH-3 (H2M2): principal component of LDH from lymphatic tissue
– LDH-4 (HM3), LDH-5 (M4): predominate in skeletal muscle or liver LDH
Note: The M subunit of LDH used to be designated the A subunit; the H subunit was the B subunit. Under the old designation, LDH-1 isoenzyme was LDH-B4.
Unit Definition
One unit (U) lactate dehydrogenase will reduce 1 mol of pyruvate to L-lactate in 1 minute at +25 °C and pH 7.0.
Note: For preparations H and I, the activity is defined at +30 °C, pH 7.8. The above assay consumes 1 mol of NADH per mol of pyruvate reduced.
Unit Conversion: For the reduction reaction, pyruvate as substrate:
1 U (+25 °C) 1.5 U (+37 °C) [hog muscle LDH in glycerol].
1 U (+25 °C) 1.8 U (+37 °C) [hog muscle LDH in amm onium sulfate].
1 U (+25 °C) 1.1 U (+30 °C) 2.0 U (+37 °C) [rabbit muscle LDH].
1 U (+25 °C) 1.4 U(+30 °C) 2.5 U (+37 °C) [pig heart LDH].
1 U (+25 °C) 2.5 U (+37 °C) [beef heart LDH].
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 7
Preparation Note
Activator: 2-amino-2-methyl-1-propanol, diethanolamine, fluoride and heparin (oxidation reaction). Sodium sulfite (Na2SO3) will protect the enzyme from the effects of thiol-attacking reagents. Mercaptans can reverse the inhibitory effects of thiol-attacking reagents.
Analysis Note
Absorbance:The purified enzyme from beef heart has an absorbance of 14.9 (10 mg/ml, 280 nm).

For life science research only. Not for use in diagnostic procedures.

form	suspension
Quality Level	100,
specific activity	~550 units/mg protein (at 25 °C (1,100 U/mg at 37 °C) with pyruvate as the substrate.)
packaging	pkg of 10 mL (10127884001 [100 mg]), pkg of 2 mL (10127230001 [10 mg]), pkg of 5 mL (10127876001 [25 mg])
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

L-Lactate Dehydrogenase (L-LDH) 10107085001

1.1.1.27 ( BRENDA | IUBMB )

L-Lactate Dehydrogenase (L-LDH) is an enzyme which is involved in the glycolytic pathway. It is believed to consist of five isotypes, LDH1 to LDH5. It also has a major role in carbohydrate metabolism of human malaria parasites.

Reduction of α-ketoacids to α-hydroxycarboxylic acids or reverse reaction.
It was used in determination of D-Lactate.
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.5

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~550 units/mg protein (at 25 °C (1,000 U/mg at 37 °C) with pyruvate as the substrate.)
packaging	pkg of 10 mL (100 mg)
Торговая марка	Roche

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

L-Malate Dehydrogenase (L-MDH)

1.1.1.37 ( BRENDA | IUBMB )

L-malate:NAD+ oxidoreductase
The L-malate dehydrogenase enzyme is a nuclear gene product that is synthesized with a 24-residue amino-terminal signal peptide. This peptide is proteolytically cleaved during the translocation of the enzyme to the mitochondrial matrix.

The enzyme L-malate dehydrogenase from pig heart has been used to measure PEPCK (phosphoenolpyruvate carboxykinase) activity. The oxaloacetate, produced by PEPCK, is reduced to malate via the oxidation of NADH, which in turn is measured at 340 nm using a spectrophotometer. The enzyme has also been used to measure oxaloacetate by measuring the reduction in NADH spectroscopically at 340 nm.
Biochem/physiol Actions
The enzyme L-malate dehydrogenase from pig heart catalyzes the oxidation of L-malate to oxaloacetate. The enzyme is an NAD-dependent mitochondrial dehydrogenase that functions in the tricarboxylic acid cycle. It is a component of the malate-aspartate shuttle that transports reducing equivalents across the inner mitochondrial membrane in the form of malate.
Quality
Contaminants: <0.002% GOT, <0.01% fumarase and LDH, each luM each, <0.02% HK, <0.002% PGI
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Solution in 50% glycerol (v/v), pH approximately 7
Preparation Note
Activator: – phosphate
– arsenate
– Mg2+
– Zn2+

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution, suspension
specific activity	~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)
packaging	pkg of 1 mL (10127248001 solution), pkg of 1 mL (10127256001 suspension), pkg of 5 mL (10127914001suspension)
Торговая марка	Roche
optimum pH	7.4-7.5(reduction of oxaloacetate), 9.2-9.5(oxidation of malate)
shipped in	wet ice
storage temp.	2-8°C

Laminin 11243217001

CAS Number:	114956-81-9
MDL number:	MFCD00081739

Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from , and chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.

Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 µg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Quality
Purity: 90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Sequence
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
Physical form
Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane
Preparation Note
Working concentration: 2 to 5 µg/cm2
2 to 5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
sterility	sterile; sterile-filtered
assay	90% (SDS-PAGE)
form	solution
mol wt	900 kDa
packaging	pkg of 1 mg (2 ml)
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	Q61292,
shipped in	dry ice
storage temp.	20°C
Gene Information	mouse ... Lamb2(16779)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

Specificity
Leupeptin inhibits serine and thiol proteases such as trypsin, plasmin, proteinase K, kallikrein, papain, thrombin, and cathepsin A and B.
Not affected are -, -, - and - chymotrypsin, pepsin, cathepsin D, elastase, renin, and thermolysin.

Leupeptin

Empirical Formula (Hill Notation):	C₂₀H₃₈N₆O₄ · 0.5H₂O₄S
Linear Formula:	C₂₀H₃₈N₆O₄ · 1/2H₂SO₄
CAS Number:	103476-89-7
Molecular Weight:	475.59
MDL number:	MFCD00037012
PubChem Substance ID:	329817679

Leupeptin as well as other protease inhibitors like antipain, chymostatin, pepstatin, and phosphoramidon are useful for the protection of proteins during their isolation from tissues or membranes. Leupeptin can be removed from the reaction by dialysis.
Note: To check other protease inhibitors, try our Protease Inhibitor Set including Antipain Dihydrochloride, Aprotinin, Bestatin, Chymostatin, E-64, EDTA-Na2, Leupeptin, Pefabloc SC, Pepstatin, and Phosphoramidon.
Biochem/physiol Actions
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
Features and Benefits
Contents
Synthetic, white powder
Quality
Function test: Performance controlled with trypsin.
Preparation Note
Working concentration: 0.5 to 5 µg/ml (1 to 20 µM)
Comparison of working concentrations of pefabloc with leupeptin and PMSF is in files.
Working solution: Solvent is recommended in distilled water.
Highly soluble in water (1 mg/ml), methanol, ethanol, acetic acid, dimethyl formamide and dimethyl sulfoxide.
Poorly soluble in acetone, chloroform, ethyl ether and n-hexane.
Storage conditions (working solution): -15 to -25 °C
In aqueous solution leupeptin is stable for 1 month at 2 to 8 °C or for at least 6 months at - 15 to -25 °C, stored under nitrogen. For best results, freeze the dissolved inhibitor in aliquots and avoid repeated thawing.
Reconstitution
Highly soluble in water (1 mg/ml). Stable for at least one week at 2 to 8 °C and 6 months frozen in aliquots at -15 to -25 °C.
Analysis Note
Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
biological source	synthetic
assay	96.5%
form	powder
mol wt	M_r 475.6
packaging	pkg of 100 mg (11529048001), pkg of 25 mg (11017128001), pkg of 5 mg (11017101001), pkg of 50 mg (11034626001)
Торговая марка	Roche
solubility	H₂O: 50 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
InChI	1S/2C20H38N6O4.H2O4S/c21-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h211-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
InChI key	CIPMKIHUGVGQTG-VFFZMTJFSA-N

The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
Liberase enzyme technology comprises methods for purifying clostridial collagenase isoforms to high specific activity, and for blending them together with high-specific-activity neutral protease in optimal ratios for effective dissociation of primary tissues and cultured cells. Liberase DH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a high concentration of Dispase®, a non-clostridial neutral protease.
Liberase enzymes are designed to increase the quality and reproducibility of tissue dissociation, and improve the viability and functionality of isolated cells. Liberase Purified Enzyme Blends replace traditional collagenase, which is a crude and variable fermentation by-product of Clostridium histolyticum.

Liberase™ DH Research Grade

Liberase™ DH (Dispase High) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
It has been used for the digestion of metastatic tumor fragments. It has been used for the enzymatic detachment of human embryonic stem cells colonies.
Features and Benefits

Maximize viability and yield of isolated cells

with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.

Count on higher specific activity of the enzyme blend

as a result of higher Collagenase I + II purity (determined by HPLC analysis).

Obtain higher experimental reproducibility

For life science research only. Not for use in diagnostic procedures.

This product is intended for life science research and in vitro use only. These products are not to be used for diagnostic or clinical applications, such as human islet transplantation.
All 1st generation Liberase and Liberase Blendzyme products (Liberase HI, CI, RI, PI, Liberase Blendzyme 1, 2, 3, 4) have been replaced by the 2nd generation Liberase Research Grade and Liberase GMP grade enzyme blend portfolios. For instructions concerning the transition from previously used Liberase Enzymes

Dispase is a registered trademark of Godo Shusei Co., Ltd.
Liberase is a trademark of Roche

Quality Level	100,
form	lyophilized
packaging	pkg of 10 mg (05401054001 [2 x 5 mg]), pkg of 100 mg (05401089001 [2 x 50 mg])
Торговая марка	Roche
parameter	35-37 °C optimum reaction temp.
optimum pH	7.4
shipped in	dry ice
storage temp.	20°C

Liberase™ DH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a high concentration of Dispase®, a non-clostridial neutral protease. Liberase™ DL (Dispase Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.

Liberase™ DL Research Grade

Liberase® DL (Dispase Low) Research Grade has been used for the isolation of skin stem cells from mice. It is also used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Features and Benefits

Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis).
Obtain higher experimental reproducibility due to higher lot-to-lot consistency.

For life science research only. Not for use in diagnostic procedures.

Dispase is a registered trademark of Godo Shusei Co., Ltd.
Liberase is a trademark of Roche
Roche is a registered trademark of Roche Molecular Systems, Inc.

Quality Level	100,
form	lyophilized
packaging	pkg of 100 mg (05466202001 [2 x 50 mg]), pkg of 10 mg (05401160001 [2 x 5 mg])
Торговая марка	Roche
parameter	35-37 °C optimum reaction temp.
optimum pH	7.4
shipped in	dry ice
storage temp.	20°C

Liberase TH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Thermolysin, a non-clostridial neutral protease.

Liberase™ TH Research Grade

Liberase™ TH (Thermolysin High) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
Features and Benefits

Maximize viability and yield of isolated cells

with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.

Count on higher specific activity of the enzyme blend

as a result of higher Collagenase I + II purity (determined by HPLC analysis).

Obtain higher experimental reproducibility

due to higher lot-to-lot consistency.

Increase safety

For life science research only. Not for use in diagnostic procedures.

Liberase is a trademark of Roche

Quality Level	100,
form	lyophilized
packaging	pkg of 10 mg (05401135001 [2 x 5 mg]), pkg of 100 mg (05401151001 [2 x 50 mg])
Торговая марка	Roche
parameter	35-37 °C optimum reaction temp.
optimum pH	7.4
shipped in	dry ice
storage temp.	20°C

Liberase™ TL Research Grade 5401020001

The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.
Liberase Purified Enzyme Blends
Liberase enzymes are blends of highly purified enzymes, designed to increase the quality and reproducibility of tissue dissociation, and improve the viability and functionality of isolated cells. Liberase Purified Enzyme Blends replace traditional collagenase, which is a crude and variable fermentation by-product of Clostridium histolyticum.
Liberase Enzyme Technology
Liberase enzyme technology comprises methods for purifying clostridial collagenase isoforms to high specific activity, and for blending them together with high-specific-activity neutral protease in optimal ratios for effective dissociation of primary tissues and cultured cells.
For additional information, such as manufacturing, technology, or storage, see the Liberase Research Grade Insightsdocument. Further reading on the topic can also be found in the Documents tab.
Roche provides special-quality preparations of Liberase Purified Enzyme Blends, manufactured to meet GMP requirements. For more information on the GMP-grade preparations.
Important Notes:

This product is intended for life science research and in vitro use only. These products are not to be used for diagnostic or clinical applications, such as human islet transplantation.
All 1st generation Liberase and Liberase Blendzyme products (Liberase HI, CI, RI, PI, Liberase Blendzyme 1, 2, 3, 4) have been replaced by the 2nd generation Liberase Research Grade and Liberase GMP grade enzyme blend portfolios. For instructions concerning the transition from previously used Liberase Enzymes.

Liberase™ TL (Thermolysin Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.
This product has been used for the isolation of pancreatic islets from mice.

Liberase TL Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a low concentration of Thermolysin, a non-clostridial neutral protease.

Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purit(determined by HPLC analysis)
Obtain higher experimental reproducibility due to higher lot-to-lot consistency.
Increase safety with an enzyme that is free of any mammalian or avian tissue-derived raw materials.

Preparation Note

Stabilizers: Calcium
Working concentration: Liberase Research Grade Enzyme Working Concentration

Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.
When the application is on the Roche list of applications at www.collagenase.com, use the Liberase Research Grade concentration recommended for that application.
When the application is not included on this list, first use Liberase TM Research Grade at a concentration of 0.08–0.28 Wnsch units/ml.
The goal is to determine the best starting concentration of Liberase Research Grade Enzyme Blends. This is a starting point, and the final concentration may vary due to differences in procedure and lot-to-lot differences in traditional collagenase.
Collagenase Working Concentration
Multiply your previous collagenase working concentration (mg/ml) by its specific activity (Wnsch units/mg, as determined above), to obtain Wnsch units/ml. To determine how much Liberase Research Grade Enzyme Blend to use, first multiply your collagenase working concentration (in Wnsch units/ml) times the total volume of your working enzyme solution to obtain the total collagenase activity needed (Wnsch units). Divide the total collagenase activity required by the Liberase Research Grade stock concentration ( “Reconstitution and Storage”). This indicates how many milliliters of Liberase Research Grade Enzyme Blend stock solution to use in your working enzyme solution.
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C. Note: Avoid repeated freezing and thawing!

Reconstitution

Reconstitute the lyophilized enzyme with injection-quality sterile water or tissue-dissociation buffer. Do not add serum or other components that may influence enzyme activity, such as albumin or protease inhibitors, to the dissociation buffer. Enzyme stability is reduced at higher concentrations and warmer temperatures (4 °C). Avoid both the above conditions.
Reconstitute the entire vial. Do not weigh individual aliquots of the lyophilizate. The introduction of moisture into the vial results in a decline in enzymatic activity.
Place vial on ice to rehydrate the lyophilized enzyme.
Gently agitate the vial at 2 to 8 °C until enzyme is completely dissolved (max. 30 min).
Depending on the type of tissue-dissociation buffer used to dissolve Liberase Research Grade Purified Enzyme Blends, slight precipitations may be observed which readily dissolve in the diluted working solution and have no influence on enzyme activity.
Remove an aliquot of the stock solution to prepare the working solution
Reconstitution volume

2 ml (1 vial with 5 mg–10 mg pack size), 10 ml (1 vial with 50 mg–100 mg pack size)
Collagenase Wnsch (units/ml)
13 (1 vial with 5 mg–10 mg pack size), 26 (1 vial with 50 mg–100 mg pack size)
Total Collagenase concentration mg/ml
2.5 (1 vial with 5 mg–10 mg pack size), 5.0 (1 vial with 50 mg–100 mg pack size)

Storage and Stability

Store at -15–-25 °C. (Store dry. Please note that the expiration date is not printed on the individual vials.)

For life science research only. Not for use in diagnostic procedures.

Liberase is a trademark of Roche

Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.

Quality Level	100
form	lyophilized
packaging	pkg of 10 mg (2x5 mg)
mfr. no.	Roche
parameter	35-37 °C optimum reaction temp.
optimum pH	7.4
shipped in	dry ice

Liberase™ TM Research Grade

Liberase™(Thermolysin Medium) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.

Stabilizers: Calcium
Working concentration: Liberase Research Grade Enzyme Working Concentration
Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.When the application is on the Roche list of applications at www.collagenase.com, use the Liberase Research Grade concentration recommended for that application.
When the application is not included on this list, first use Liberase TM Research Grade at a concentration of 0.08–0.28 Wnsch units/ml.The goal is to determine the best starting concentration of Liberase Research Grade Enzyme Blends. This is a starting point, and the final concentration may vary due to differences in procedure and lot-to-lot differences in traditional collagenase.
Collagenase Working Concentration
Multiply your previous collagenase working concentration (mg/ml) by its specific activity (Wnsch units/mg, [as determined above]), to obtain Wnsch units/ml. To determine how much Liberase Research Grade Enzyme Blend to use, first multiply your collagenase working concentration (in Wnsch units/ml) times the total volume of your working enzyme solution to obtain the total collagenase activity needed (Wnsch units). Divide the total collagenase activity required by the Liberase Research Grade stock concentration ( “Reconstitution and Storage”). This indicates how many milliliters of Liberase Research Grade Enzyme Blend stock solution to use in your working enzyme solution.
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C. Note: Avoid repeated freezing and thawing!

Maximize viability and yield of isolated cells: with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
Count on higher specific activity of the enzyme blend: as a result of higher Collagenase I + II purity (determined by HPLC analysis).
Obtain higher experimental reproducibility: due to higher lot-to-lot consistency.
Increase safety: with an enzyme that is free of any mammalian or avian tissue-derived raw materials.

Preparation Note

Reconstitution

Reconstitute the lyophilized enzyme with injection-quality sterile water or tissue-dissociation buffer. Do not add serum or other components, such as albumin or protease inhibitors, to the dissociation buffer. Enzyme stability is reduced at higher concentrations and warmer temperatures (4 °C). Avoid both the above conditions.
Reconstitute the entire vial. Do not weigh individual aliquots of the lyophilizate. The introduction of moisture into the vial results in a decline in enzymatic activity.
Place vial on ice to rehydrate the lyophilized enzyme.
Gently agitate the vial at 2 to 8 °C until enzyme is completely dissolved (max. 30 min).
Depending on the type of tissue-dissociation buffer used to dissolve Liberase Research Grade Purified Enzyme Blends, slight precipitations may be observed which readily dissolve in the dilutedworking solution, and have no influence on enzyme activity.
Remove an aliquot of the stock solution to prepare the working solution.
Reconstitution volume
2 ml (1 vial with 5 mg–10 mg pack size), 10 ml (1 vial with 50 mg–100 mg pack size)
Collagenase Wnsch (units/ml)
13 (1 vial with 5 mg–10 mg pack size), 26 (1 vial with 50 mg–100 mg pack size)
Total Collagenase concentration [mg/ml]
2.5 (1 vial with 5 mg–10 mg pack size), 5.0 (1 vial with 50 mg–100 mg pack size)

For life science research only. Not for use in diagnostic procedures.

Liberase is a trademark of Roche

Quality Level	100
form	lyophilized
packaging	pkg of 10 mg (05401119001 [2 x 5 mg]), pkg of 100 mg (05401127001 [2 x 50 mg])
Торговая марка	Roche
parameter	35-37 °C optimum reaction temp.
optimum pH	7.4
shipped in	dry ice
storage temp.	20°C

Lumi-Light Western Blotting Substrate 12015200001

Lumi-Light Western Blotting Substrate has been used in western blotting.
Lumi-Light Western Blotting Substrate is a chemiluminescent POD substrate for western blots. Routine detection of any antigen blotted on a PVDF or nitrocellulose membrane. It is suited for high-sensitivity routine western blotting, especially when quantification is required. The chemiluminescent signal persists for more than 3 hours, allowing multiple exposures to be taken.
Features and Benefits

Detect rare proteins: Lumi-Light substrate detects antigen in the range of 10 - 50pg.
Multiple exposures possible: The signal is stable for more than three hours after substrate addition.
Save sample material: Lumi-Light substrate detects less than 50-pg amounts of antigen.
Save primary antibody: Due to the strong signal of the Lumi-Light substrate, primary antibody can be diluted up to 10-fold more than with colorimetric detection systems.
Easy preparation of substrate solution: Just mix the two Lumi-Light components in a ratio of 1:1.

Contents
Lumi-Light Luminol/Enhancer Solution, 2 x 100ml
Lumi-Light Stable Peroxide Solution, 2 x 100ml
Packaging
1 kit containing 2 components
Quality
Each lot is function tested using mouse or rabbit primary antibodies.
Specifications
Number of Tests: The reagent is sufficient for 40 blots, each with a size of 10cm x 10cm.
Sensitivity: Depending on the affinity of the primary antibody, 10 - 50pg of antigen can be detected

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
packaging	pkg of 400 mL
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Lumi-LightPLUS Western Blotting Substrate 12015196001

As the technology for quantifying chemiluminescence advances, the substrates for chemiluminescence must also advance. For instance, the light emission of conventional enhanced luminol substrates substantially decreases within 30 minutes. In contrast, the chemiluminescence signal of the Lumi-LightPLUS substrate persists for more than nine hours, and since exposures of only a few minutes are necessary, multiple exposures can be taken. Additionally, the ability to take extended exposures allows the detection of rare proteins, or higher dilutions of antigen or antibody to be used. The emission wavelength of the Lumi-LightPLUS substrate is 425 nm.
Comparison of Lumi-Light Substrate and Lumi-Light PLUS Substrate
Lumi-Light Substrate Lumi-LightPLUS Substrate
Sensitivity 10-50 pg antigen 1-5 pg antigen
Pimary Antibody Dilution Dilute up to 10-fold more compared to colorimetric detection Dilute up to 100-fold more compared to colorimetric detection
Signal Stability >3 hours (enhance signal with a 30-minute incubation) > 9 hours (enhance signal with a 30-minute incubation)
Number of tests: The reagent is sufficient for 10 blots, each with a size of 10 cm x 10 cm.
Sensitivity: Depending on the affinity of the primary antibody, 1 - 5 pg of antigen can be detected in research studies.
Storage/stability: The Lumi-LightPLUS substrate components are stable at +2 to +8°C until the expiration date printed on the label. Components are shipped at +15 to +25°C.

Lumi-LightPLUS Western Blotting Substrate has been used in western blotting.
Lumi-LightPLUS Western Blotting Substrate is for the chemiluminescent detection of antigens blotted on membranes using horseradish peroxidase-conjugated secondary antibodies. It can be used for very sensitive detection of any antigen blotted on a PVDF Western Blotting Membrane or nitrocellulose membrane.
Lumi-LightPLUS Western Blotting Substrate is suited for high-sensitivity western blotting, especially when quantification is required. The chemiluminescent signal persists for more than 9 hours, allowing multiple exposures to be taken.
Features and Benefits

Detect rare proteins. Detect antigen in the range of 1 - 5 pg.
Take multiple exposures. The signal is stable for more than nine hours after substrate addition.
Save sample material. Lumi-LightPLUS substrate detects less than 5 pg-amounts of antigen.
Save valuable resources. Dilute primary antibody 10- to 100-fold more than with colorimetric detection systems.
Benefit from easy preparation. Simply mix the two Lumi-Light components in a 1:1 ratio.

Packaging
1 kit containing 2 components
Quality
Each lot is function tested using mouse or rabbit primary antibodies.
Preparation Note
Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

Dot blot: 50 mU/ml
ELISA: 25 mU/ml
Western blot: 50 mU/ml

Working solution: Preparation of Additional Reagents and Solutions
The table inside the package insert describes the preparation of working solutions. Volumes are designed for a membrane of 10x10 cm. If larger membranes are used, volumes have to be scaled up. For reproducible results, equilibrate all solutions to room temperature before use.
Note: Do not use Azide to stabilize the solutions against microbial growth, as azide irreversibly inhibits horseradish peroxidase.

For life science research only. Not for use in diagnostic procedures.

packaging	pkg of 100 mL (1,000 cm² membrane)
Quality Level	100,
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Lysozyme 10837059001

3.2.1.17 ( BRENDA | IUBMB )

Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase

Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.
Lysozyme has been used for:

Cell wall degradation
Preparation of protoplasts
Bacteriolysis
Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
Food and drinks (flavor enhancer)
Sample preparation prior to isolation of nucleic acids

Biochem/physiol Actions
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.
Unit Definition
One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.
Preparation Note
Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.
Reconstitution
Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	95%
specific activity	>23000 U/mg (Shugar units)
mol wt	14.4 kDa
packaging	pkg of 10 g
Торговая марка	Roche
optimum pH	6.0-7.0
absorption	3.9 at 280 nm (10 mg enzyme/ml)

Mouse monoclonal antibody (Clone M30, mouse IgG2b) is used for the detection of apoptosis in epithelial cells (caspase cleavage product of cytokeratin 18). It is provided in lyophilized, stabilized, formalin grade form.
During apoptosis, vital intracellular proteins are cleaved. The proteases that mediate this process are called caspases (Cysteinyl-aspartic acid proteases). Caspases are expressed as zymogenes, which are activated by different apoptosis inducers. Once activated, a single caspase activates a cascade of caspases. Until recently, cytokeratins, in particular cytokeratin 18 (CK18), were not known to be affected by early events of apoptosis. Recently, it has been shown that the M30 antibody recognizes a specific caspase-cleavage site within cytokeratin 18, which is not detectable in native CK18 of normal cells. Consequently, the M30 CytoDEATH antibody is a unique tool for the easy and reliable determination of very early apoptotic events in single cells and tissue sections.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

M30 CytoDEATH

Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing)
Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections)

Specificity
The M30 CytoDEATH antibody binds to a caspase-cleaved, formalin-resistant epitope of the cytokeratin 18 (CK 18) cytoskeletal protein. The immunoreactivity of the antibody is confined to the cytoplasm of apoptotic cells.

The M30 CytoDEATH antibody is used for the determination of early apoptotic events in cells and tissue sections by detection of a specific epitope of cytokeratin 18 that is presented after cleavage by caspases. Detect apoptosis by applying the M30 antibody to fixed samples, then using secondary detection systems suitable for immunohistochemistry, immunocytochemistry, and flow cytometry.
Features and Benefits

Convenient: Easy-to-use standard protocol
Clear results: Apoptotic cells are most easily distinguishable from normal cells
Early detection of apoptosis: Detects caspase-cleaved Cytokeratin 18; caspase activity is one of the earliest apoptosis markers
Useful for paraffin-embedded tissue: Useful for routinely fixed tissue samples; retrograde studies are possible

Physical form
White lyophilizate. The antibody has been lyophilized in the presence of proteinous stabilizers.
Preparation Note
Short protocol
For formalin-embedded tissue:

Dewax formalin-fixed, paraffin-embedded tissue sections.
Retrieve antigen by heating in citric-acid buffer.
Add M30 antibody.
Add Anti-mouse-biotin.
Add Streptavidin-POD.
Add substrate solution (DAB or AEC).
Counterstain with Harris hematoxylin.
Analyze under a light microscope.

For immunofluorescence on cells:

Fix cells.
Add M30 antibody.
Add Anti-mouse-Ig-fluorescein.
Analyze under a fluorescence microscope.

Working concentration: The concentration of the stock solution is 6,6 ng/ µl. For the QC-release test of this product, a function test using a cellular model (HeLa cells treated with TNF-/Actinomycin D) is performed, as mentioned in the pack insert. The quantity of protein is only part of the QC-release test of the stock product that is why it is not included in the documentation.
Working solution: Procedure for Immunofluorescence in Cells and Flow Cytometry

When using other detection methods or sample material, conditions may vary and have to be adapted.
For application, dilute the antibody stock solution in Incubation buffer (M30 CytoDEATH).
Note: The antibody solutions should be free of precipitate. If necessary, centrifuge the solutions at high speed prior to use.
Additional working solutions required for the immunofluorescence and flow cytometry procedure:
Washing buffer: PBS containing 0.1% Tween 20
Incubation buffer: PBS containing BSA and 0.1% Tween 20
Procedure for Immunohistochemistry

When using other detection methods or sample material, the conditions may vary and have to be adapted.
Dilute the antibody stock solution in Incubation buffer.
Note: The antibody solutions should be free of precipitate. If necessary, centrifuge the solution at high speed prior to use.
Working solutions need to perform the immunohistochemistry staining procedure:
Washing buffer: PBS containing 0.1% Tween 20
Incubation buffer: PBS containing BSA and 0.1% Tween 20
Citric acid buffer: 2 g/l citric acid, pH 6 adjusted with 1 N NaOH
Storage conditions (working solution): The antibody stock solution is stable for 6 months at 2 to 8 °C. Alternatively, it can be stored in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Reconstitution
Reconstitute lyophilizate in 550 μl to obtain an antibody stock solution.

For life science research only. Not for use in diagnostic procedures.

The M30 antibody is made under a license agreement from Peviva AB, Sweden.

Quality Level	100,
form	lyophilized (clear, colorless solution after reconstitution)
usage	sufficient for 250 tests (12140349001), sufficient for 50 tests (12140322001)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

Mae III is a restriction enzyme which recognizes the sequence ↓GTNAC and generates fragments with 5′-cohesive termini.
Specificity
Recognition sites: GTNAC
GTNAC
Restriction site: GTNAC
GTNAC
Heat inactivation: There is no information available whether or not Mae III can be heat inactivated.

Mae III

Mae III has been used as a restriction enzyme for PCR products.
Quality
Absence of nonspecific endonuclease activities
1µg DNA is incubated for 16hours in 50µl incubation buffer with an excess of Mae III. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.

Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Mae III for 4hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs

: 156
X174: 17
Ad2: 118
M13mp7: 25
pBR322: 17
pBR328: 18
pUC18: 11
SV40: 14

Unit Definition
One unit is the enzyme activity that completely cleaves 1 µg DNA in one hour at +55 °C in a total volume of 25 µl (1x) special Mae I incubation buffer.
Analysis Note
Compatible ends
Mae III ends are compatible with ends generated by Bst E II.

Isoschizomers
The enzyme has no known isoschizomers.
Methylation sensitivity
There is no evidence that the enzyme is inhibited by methylation.

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 0-10%
B: 10-25%
H: 10-25%
L: 0-10%
M: 0-10%

Note: Instead of the SuRE/Cut buffer system, please use the special 2x incubation buffer supplied with the enzyme.
Incubation temperature
+55°C

Unit definition
One unit is the enzyme activity that completely cleaves 1µg DNA in 1 hour at +55°C in a total volume of 25µl special incubation buffer.

Heat inactivation
No information available.
Ligation and recutting assay
Mae III fragments obtained by complete digestion of 1µg DNA are ligated with 1U T4 DNA Ligase in a volume of 10µl by incubation for 16 hours at +4°C in 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +8°C) resulting in >95% recovery of 1µg DNA fragments.
Subsequent re-cutting with Mae III yields >95% of the typical pattern of DNA x Mae III fragments.
Activity in PCR buffer: 0%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 0%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 µM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 250 U (10822248001 [1 - 5 U/µl]), pkg of 50 U (10822230001 [1 - 5 U/µl])
Торговая марка	Roche
parameter	55 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C