Roche

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5 ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.

DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Quality
The DNA shows the typical cleavage pattern for wild type DNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequence
Chain Length 48,502 bp
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Storage and Stability
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
mol wt	32,300 kDa
packaging	pkg of 1 mL
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

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11467140001

DNA, MB-grade 11467140001

General description
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Sequence
Chain Length 120 to 3,000 nucleotides
Physical form
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note
Working concentration: 100µg/ml
This fish sperm DNA should be used at a concentration of 100 µg/ml (1 : 100 dilution) in prehybridization solutions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Параметры

Quality Level 100,

form solution (ready-to-use)

mol wt 40-1,000 kDa

packaging pkg of 500 mg

Торговая марка Roche

shipped in dry ice

storage temp. −20°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany nwg

Flash Point F No data available

Flash Point C No data available

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DNase I 10104159001

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-

The isolation of cells from lung, skin and tumor samples
Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

Unit Definition

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Preparation Note

Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	lyophilized
packaging	pkg of 100 mg
Торговая марка	Roche
optimum pH	~7.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I 11284932001

3.1.21.1 ( BRENDA | IUBMB )

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	lyophilized
packaging	pkg of 100 mg
Торговая марка	Roche
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I recombinant 4536282001

3.1.21.1 ( BRENDA | IUBMB )

Double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Properties: Double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Activity: 10,000U/bottle according to Kunitz (+25°C; DNA as substrate).
Unit definition: One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minutes under assay conditions.

Features and Benefits
Contents
Lyophilizate
Unit Definition
One unit, according to Kunitz, is the enzyme activity that under assay conditions causes an absorbance increase at 260 nm of 0.001/minute in 1 ml. Volume activity is determined in 0.1 M sodium acetate, 5 mM MgSO4, pH 5.0.
Assay mixture: Calf thymus DNA (100 µg) is incubated with 20 to 50 U DNase I at +25 °C.
The increase in absorbance is measured at 260 nm.
Information Note: These conditions are used for the determination of activity,according to Kunitz, since the Kunitz assay results in high reproducibility and sensitivity. When using this enzyme for normal experimental purposes, Roche recommends using incubation buffers that are appropriate for a given application, e.g., as mentioned in the standard literature (Sambrook et al., Curr. Prot. Mol. Biol., etc.).
Preparation Note
Activator: The enzyme requires divalent cations for maximal activity. The specificity of the reaction depends on the nature of the cation used. In the presence of Mg2+ DNase I causes single-stranded nicks in dsDNA, while in the presence of Mn2+ the enzyme produces double-stranded breaks.
Storage conditions (working solution): Long-term Storage of the Dissolved Enzyme
The solvent generally recommended for DNase I is water. When DNase I is reconstituted in water, the solution can be kept for 2 days at 2 to 8 °C and for 1 month at -15 to -20 °C. For best results, prepare appropriate aliquots and avoid repeated freezing and thawing. However, for long-term storage, carefully dissolve DNase I in one of the buffers listed below. In buffer 1, the enzyme will be stable for several weeks. The enzyme will be stable for at least 18 months if it is dissolved in buffer 2, 3, or 4 and stored at the recommended temperatures.
Information note: Do not vortex the enzyme while dissolving!
Buffer 1: 20 mM Tris-HCl, 20 mM MgCl2, 5 mM CaCl2, 0.1 mM dithioerythritol, 0.1 mM EDTA, 50% (v/v) glycerol, pH 8. Store this solution at -15 to-25 °C; it will not freeze at this temperature and will be stable for several weeks.
Buffer 2: 20 mM Tris-HCl, 50 mM NaCl, 1 mM dithioerythritol, 100 µg/mL BSA, 50% glycerol (v/v), pH 7.6. Store this solution at -15 to -25 °C for up to 18 months; it will not freeze at this temperature.
Buffer 3: 20 mM Tris-HCl, 1 mM MgCl2, 50% (w/v) glycerol, pH 7.5. Store this solution at -15 to -25 °C for up to 18 months; it will not freeze at this temperature.
Buffer 4: 20 mM Tris-HCl, 1 mM MgCl2, pH 7.5. Aliquot in appropriate amounts (e.g., 10 µl), freeze the aliquots quickly on dry ice, and store at -60 °C or below for up to 18 months. Thaw only the amount needed for each experiment. Do not refreeze. Discard any leftover thawed solution.

DNase I, recombinant, grade I is used for:Eliminating DNA during protein isolation procedures
Analysis of chromatin structure
Eliminating DNA during sample preparation

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 2 x 10,000 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I recombinant, RNase-free 4716728001

3.1.21.1 ( BRENDA | IUBMB )

Recombinant DNase I is a DNA-specific endonuclease.The enzyme catalyzes the degradation of both double- and single-stranded DNA randomly by hydrolyzing phosphodiester linkages to DNA, resulting in a mixture of oligo- and mononucleotides. All material used during the production process of DNase I recombinant is non-animal sourced, resulting in an animal-free product.
Contents

Recombinant DNase I, RNase-free, 10 U/µl
Incubation Buffer, 10x concentrated

Specificity

Heat inactivation: One unit DNase I recombinant, RNase-free is heat-inactivated by 10 minutes incubation at 75 °C.
Important Note: Alternatively, DNase I recombinant, RNase-free can be inactivated and removed by phenol extraction according to standard protocols, e.g., Current Protocols in Molecular Biology.

DNase I recombinant, RNase-free may be used to degrade DNA in applications that are sensitive to the presence of RNase. For example, DNase I is frequently used to:

Remove genomic DNA from RNA preparations prior to RT-PCR
Isolate DNA-free RNA after in vitro transcription reactions
Perform nick translations
Map DNase-sensitive regions in eukaryotic DNA

Features and Benefits

Eliminates DNA contamination from any RNA sample
Contains no detectable RNase or protease activity
Can be heat inactivated, thereby eliminating the need for organic extraction
Shipped with an optimized incubation buffer, which supports maximum DNase activity
Produced via an entirely animal-free process, to eliminate any risks associated with animal-derived material

Packaging

1 kit containing 2 components

Quality

Absence of contaminants: Each lot is tested to ensure the absence of RNases and proteases according to the current Quality Control procedures.

Specifications

Glycosylated form
Recombinant DNase I is heterogeneously N-glycosylated, so it appears as two bands in gel electrophoresis.
Divalent ion requirement
DNase I requires divalent cations for maximum activity. The DNA-specific endonuclease is activated by ions such as magnesium ions and is stimulated by calcium ions. Therefore, the enzyme is inhibited by metal chelating agents like EDTA.

Unit Definition

One unit is the enzyme activity that effects an absorbance increase of 0.001/minute under assay conditions in 1 ml at 260 nm.
Assay conditions:
Volume activity is determined according to the following assay mixture. 100 µg calf thymus DNA is incubated in 2.5 ml 1x incubation buffer with 40 to 70 units DNase I recombinant, RNase-free at +25 °C. The absorbance increase is measured at 260 nm.

Preparation Note

Activator: Bivalent metal ions
Working solution: Storage Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, 2 mM MgCl2, 1 mM dithioerythritol, 0.1 mg/ml Pefabloc SC, 50% glycerol (v/v), pH 7.6 (at 4 °C).
Incubation Buffer (10x): 400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Enzyme Dilution Buffer: 25 mM Tris-HCl, 50% glycerol (v/v), pH 7.6 (at 4 °C).

Storage and Stability

Store undiluted enzyme solution at -15 to -25°C; storage buffer at 4 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	bovine pancreas
recombinant	expressed in Pichia pastoris
form	solution
mol wt	~39 kDa
packaging	pkg of 10,000 U
Торговая марка	Roche
optimum pH	7.0-8.0
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DOTAP Liposomal Transfection Reagent 11202375001

Empirical Formula (Hill Notation):	C₄₃H₈₃NO₈S
CAS Number:	144189-73-1
Molecular Weight:	774.19
MDL number:	MFCD16660441
PubChem Substance ID:	329749323

DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.

The DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells.
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
Features and Benefits

Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cells
Highly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran method
Gentle: No cytotoxic effects
Flexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures.
Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered.

Preparation Note
Working concentration: Approximately 5 - 10µg DOTAP/µg DNA, and 10 - 20µg DOTAP/ml cell culture medium.
The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied.
Cytotoxicity: Not cytotoxic up to a concentration of 100µg/ml (PBLs, HeLa cells).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>99% (TLC)
packaging	pkg of 5 x 400 μL (10 U/μl)
Торговая марка	Roche
application(s)	transfection: suitable
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.O=S([O-])(OC)=O
InChI	1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-;
InChI key	RSMRWWHFJMENJH-LQDDAWAPSA-M

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1µg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10µl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 x Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
Dpn I requires methylation of adenine residues for activity and thus digests only GmATC sequences containing N6-methyladenine. Such methylated sequences are produced by dam methylase. This requirement for methylation distinguishes Dpn I from Mbo I, which is inhibited by dam methylation, and Sau3A I, which is not influenced by dam methylation. Dpn I also cleaves at a different position on the recognition sequence than Mbo I and Sau3A I.
Specificity
Recognition sites: GmAT*C
GmAT*C
Restriction site: GmAT*C
GmAT*C
Heat inactivation: No inactivation of Dpn I after incubation at 65 °C for 15 minutes.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

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Dpn I

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.
Quality
Absence of nonspecific endonuclease activities
1µg pBR322 DNA is incubated for 16 hours in 50µl SuRE/Cut Buffer A with an excess of Dpn I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Dpn I for 4 hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Compatibility
Dpn I generates fragments with blunt ends that are compatible with any blunt end.
DNA Profile
Number of cleavage sites on different DNAs

: 116
X174: 0
Ad2: 87
M13mp7: 8
pBR322: 22
pBR328: 27
pUC18: 15
SV40: 8

Unit Definition
One unit is the enzyme activity that cleaves 1 µg pBR322 DNA in one hour at +37 °C in a total volume of 25 µl (1x) SuRE/Cut Buffer A. Since complete Dpn I digestion of pBR322 DNA needs fully methylated GATC sequences < 5% partial digested bands may be observed during activity determination.
Analysis Note
Activity in PCR buffer: 100%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 µM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 100%; Pwo SuperYield DNA Polymerase PCR Mix is not available in U.S.
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 100%
B: 75-100%
H: 75-100%
L: 50-75%
M: 75-100%

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1,000 U (10742988001 [10 U/µl]), pkg of 200 U (10742970001 [10 U/µl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

Specificity
The thiol protease inhibitor E-64 specifically inhibits papain and other cysteine proteases such as cathepsin B and L, bromelain, and ficin. Cathepsin A and D are not inhibited. L-lactate dehydrogenase from porcine heart is not inhibited, although the enzyme contains a functional thiol group.

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E-64

Empirical Formula (Hill Notation):	C₁₅H₂₇N₅O₅
CAS Number:	66701-25-5
Molecular Weight:	357.41
Beilstein/REAXYS Number:	1405664
MDL number:	MFCD00080261
PubChem Substance ID:	329798998

Кат. номер

11585681001

10874523001

E-64 is used for the inhibition of papain and other cysteine proteases such as cathepsin B and L, bromelain, and ficin during isolation and purification of proteins and enzymes.
It has been used in the affinity chromatography for the purification of hemoglobin receptors.
The inhibition of thiol proteases by E-64 appears to be of a non-competitive nature between the SH components. The inhibition is also irreversible, and is altered after gel filtration (Sephadex column) or dialysis after incubation of papain with E-64. The enzyme and inhibitor combine in an equimolar ratio.
E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.
Biochem/physiol Actions
E-64 is an irreversible, potent, and highly selective cysteine protease inhibitor. E-64 does not react with the functional thiol group of non-protease enzymes, such as L-lactate dehydrogenase or creatine kinase. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 is a very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition, it is permeable in cells and tissues and has low toxicity.
Preparation Note
Working concentration: 0.5 to 1.0 µg/ml (1.4 to 28.0 µM)
Working solution: Soluble to 20 mg/ml (stock solution) in a 1:1 (v/v) mixture of ethanol and water (vortexing or slight warming in water bath (40 °C) may facilitate dissolution). Also soluble in a neutral water/methanol solution, in water, methanol, acetic acid, pyridine and DMSO. Sparingly soluble in ethanol and propanol. Insoluble in acetone, chloroform, ethyl ether and benzene.
Storage conditions (working solution): -15 to -25 °C
Solutions are stable for one month when stored in aliquots at -15 to -25 °C.
E-64 is soluble in water. A 20 mg/ml solution can be prepared in water (heat may be needed). A suggested stock solution is a 1 mM aqueous solution. The effective concentration for use as a protease inhibitor is 1 to10 μM. Aqueous stock solutions are stable for months at -20 °C. Diluted solutions are stable for days at neutral pH. E-64 is stable from pH 2-10, but is unstable in ammonia or in HCl. E-64 is also soluble in DMSO, a 10 mM solution can be prepared in dry DMSO and stored at -20 °C. Subsequent dilutions were in culture medium. Solutions for injection were prepared by dissolving E-64 in 0.9% sodium chloride or in a minimum amount of saturated sodium bicarbonate followed by dilution with 0.9% sodium chloride (after adjusting the pH to 7.0 with acetic acid).
Reconstitution
Soluble to 20 mg/ml in a 1:1 (v/v) mixture of ethanol and water. Solutions are stable for one month when stored in aliquots at -15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>95% (HPLC)
mol wt	M_r 357.4
packaging	pkg of 10 mg (10874523001), pkg of 25 mg (11585681001)
Торговая марка	Roche
pH range	2 - 10
solubility	ethanol: water (1:1): soluble 20 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	CC(C)C[C@H](NC(=O)[C@@H]1O[C@H]1C(O)=O)C(=O)NCCCCNC(N)=N
InChI	1S/C15H27N5O5/c1-8(2)7-9(20-13(22)10-11(25-10)14(23)24)12(21)18-5-3-4-6-19-15(16)17/h8-11H,3-7H2,1-2H3,(H,18,21)(H,20,22)(H,23,24)(H4,16,17,19)/t9-,10+,11+/m0/s1
InChI key	LTLYEAJONXGNFG-HBNTYKKESA-N

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Endoproteinase Arg-C Sequencing Grade 11370529001

3.4.22.8 ( BRENDA | IUBMB )

Endoproteinase Arg-C Sequencing Grade is isolated from Clostridium histolyticum as a highly purified and specific cysteine protease.
Specificity
Endoproteinase Arg-C Sequencing Grade is a cysteine protease that specifically cleaves peptide, ester, and amide bonds at the C-terminal side of arginine residues. The specificity is primarily to arginine residues, although hydrolysis proceeds to a minor degree in most lysine-containing substrates. Reducing agents (such as DTT) and Ca2+ are required for full activity.
The specificity of Endoproteinase Arg-C Sequencing Grade is tested with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Endoproteinase Arg-C Sequencing Grade are incubated for prolonged incubation times to detect traces of impurities such as contaminating proteases.
Recognition sites: C-terminal side of arginine residues, other recognition sites with lower efficiency.

Use Endoproteinase Arg-C Sequencing Grade for the specific cleavage of proteins and peptides for peptide mapping, fingerprinting, and sequence analysis. The protease is suitable to digest proteins in solution, in gels, or on blotting membranes.
Quality
Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.
Preparation Note
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and -mercaptoethanol.
Inhibitors: Oxidizing agents, sulfhydryl reactants (e.g., TLCK), EDTA, Co2+, Cu2+, Cd2+. Citrate, borate, and Tris anions partially inhibit.
Optimum pH: 7.2 - 8.0
Storage conditions (working solution): -15 to -25°C
For long-term use, the solution should be gently flushed with a stream of nitrogen and stored at -15 to -25°C. The reconstituted incubation buffer is stable for several months at -15 to -25°C.
Reconstitution
The lyophilized endoproteinase Arg-C sequencing grade is reconstituted in 50 µl double-dist. water to give a final concentration of 50 mM Tris-HCl buffer, 10 mM CaCl2, 5 mM EDTA, pH 8.0. The lyophilized activation solution is dissolved in 100 µl double-dist. water resulting in a final 50 mM dithiothreitol-(DTT-)concentration and 5 mM EDTA-concentration.

For life science research only. Not for use in diagnostic procedures.

form	lyophilized
mol wt	M_r 59 kDa
packaging	pkg of 3 x 5 µg
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
optimum pH	7.2-8.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 - H315 - H318 - H334
pcodes	P261 - P264 - P280 - P304 + P340 - P305 + P351 + P338 + P310 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Endoproteinase Glu-C (V8 Protease) 10791156001

3.4.21.19 ( BRENDA | IUBMB )

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.
Specificity
Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.
Biochem/physiol Actions
Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
Preparation Note
Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

form	lyophilized (salt-free)
specific activity	20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).), ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)
mol wt	30 kDa
packaging	pkg of 2 mg
Торговая марка	Roche
optimum pH	8.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Enterokinase

3.4.21.9 ( BRENDA | IUBMB )

Enterokinase is a protease from calf intestine and supplied in a quality optimized for the cleavage of fusion proteins.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Specificity
Serine protease acting as a restriction protease that recognizes the amino acid sequence -(Asp)4-Lys-X. The aspartic acid residues can be partially substituted by glutamic acid.

Quality
Purity: highly purified, not standardized with albumin
Quality control: function tested
Enterokinase is used for the cleavage of fusion proteins at definite cleavage sites. For the processing of recombinant proteins, the desired protein is fused with Enterokinase recognition sequence. After purification of the entire fusion protein, the protein or peptide is released by incubation with enterokinase.
Preparation Note
Working concentration: 1:50 (w/w)
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.

For life science research only. Not for use in diagnostic procedures.

form	lyophilized
mol wt	150 kDa
packaging	pkg of 3 x 250 µg (11351311001), pkg of 3 x 30 µg (11334115001)
Торговая марка	Roche
concentration	1:50 % (w/w)
optimum pH	8
shipped in	wet ice
storage temp.	2-8°C

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Epidermal Growth Factor, human (hEGF) 11376454001

Recombinant Epidermal Growth Factor, human (hEGF), is produced in E. coli and purified by standard chromatographic techniques. It is a 100µg white lyophilizate, filtered through 0.2µm pore size membrane, recombinant from E. coli.
Epidermal growth factor (EGF) is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids EGF is produced in the tubular cells of the submaxillary gland of the mouse, in the acinar cells of the human submaxillary gland, and in the human duodenal glands.
Human EGF is identical to -urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion. 37 amino acids of the 53 amino acids comprising the longer urogastrone (human EGF) and mouse EGF are common to both peptides (70% homology) and the three disulfide bonds are formed in the same relative positions. The biological activity of human EGF is similar to that of mouse EGF. The cellular receptor for EGF is the best understood growth factor receptor. The oncogene v-erbB codes for a product homologous to a portion of the EGF receptor in which the EGF-binding domain has been deleted. Evidence exists suggesting that this truncation of the EGF receptor may lead to constitutive activation without requirement for ligand binding.
EGF stimulates the proliferation and differentiation of awide variety of cells of ectodermal and mesodermal origin (e.g., fibroblasts, glial cells, keratinocytes, epithelial cells, endothelial cells, chondrocytes). EGF is a constituent of many serum-free media formulations for a variety of cells.
Specificity
Species Specificity: Human EGF is effective on human and mouse cells.
Specific activity: <0.5 ng/ml, at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Epidermal growth factor (EGF) stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, and is a constituent of many serum-free media formulations. It has been used in the cell differentiation assay.
Sequence
The primary structure of recombinant, human EGF (one polypeptide chain, 54 amino acids) is identical to that of human, natural EGF (β-urogastrone) (53 amino acids), however, recombinant EGF has an additional methionine at the amino-terminus.
Chain Length 54 AA
Unit Definition
Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)
Unit Definition: EC50 defintion: The concentration of hEGF that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with MK cells.
Physical form
White lyophilizate, filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: 0.5 to 20 ng/ml
Human EGF exerts its biological activity in the concentration range of 0.5 to 20 ng/ml. Recommended concentration for serum-free cell culture is 1 to 10 ng/ml
Working solution: In sterile water (final concentration: 500 µg/ml), further dilution with medium or PBS (phosphate buffered saline) containing 1 mg/ml BSA (bovine serum albumin), or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the reconstituted solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in E. coli
assay	>95% (HPLC)
form	lyophilized
potency	<0.5 ng/mL EC₅₀
mol wt	6200 Da
packaging	pkg of 100 µg
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
color	white
UniProt accession no.	Q6QBS2,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... EGF(1950)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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EPO ELISA 11693417001

Erythropoietin (Epo) is a cytokine belonging to the hematopoietic cytokine superfamily. It has structural similarity to growth hormone and is encoded as a 193 amino acid polypeptide comprising of canonical leader sequence composed of 27 amino acid, and a carboxyl terminal arginine which is removed by posttranslational cleavage. Furthermore, Epo is produced in adult kidney and fetal liver and is involved in stimulating red blood cell production.
Specificity
The ELISA system detects both natural and recombinant human EPO from CHO cells. No cross-reaction with other serum components has been found.

Immunogen

Recognized epitope not known.

For research use only. Not for use in diagnostic procedures.
The EPO ELISA is designed for use in life science research studies as a method for the determination of EPO concentrations in plasma and serum; it is not intended for diagnostic procedures. The literature indicates that in certain forms of anemia, specifically in aplastic renal anemia, the regulation of EPO formation in the kidney appears to be intact. This may lead to an extremely high EPO concentration in serum of the respective individuals.
Exaggerated EPO levels, accompanied by a normal or even elevated hemoglobin concentration (and/or hematocrit values), have been found in a series of diseases (e.g., tumors of the kidney or liver, or in secondary forms of polycythemia). Polycythemia vera, however, is characterized by an abnormally increased red blood cell count in tandem with a low EPO serum level. The EPO ELISA is intended to help increase scientific knowledge about these relationships.
Packaging
1 kit containing 10 components.
Unit Definition
Unit Conversion: The EPO concentration of unknown samples is calculated from a calibration curve. Each lot of standards (used for the calibration curve) is calibrated according to the 2. IRP WHO preparation (see lot-specific label). Conversion factor: 1 mlU/ml corresponds to 6.6 pg/ml. Conversion factor: 1 mU = 6.6 pg.
The normal range of a healthy human lies between 4 to 90 mIU/ml.
Preparation Note
Working solution: Solution 1 hEPO control serum (Bottle 1)
Reconstitute the lyophilizate in 500 µl double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A slightly milky solution.
Stability of Solution:

1 week at 2 to 8 °C
12 months at -15 to -25 °C

For Use in: step 1
Solution 2 Anti-hEPO-HRP (Bottle 2)
Reconstitute the lyophilizate in 500 µl double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A clear, colorless solution.
Stability of Solution:

12 months at 2 to 8 °C. Warning: do not freeze

For Use in: solution 9
Soluton 3a to 3f hEPO standard (Bottles 3a-3f)
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A clear, colorless solution.
Stability of Solution:

8 hours at 2 to 8 °C
aliquot the rest to be used for subsequent standard curves and store at -15 to -25 °C

For Use in: step 1
Solution 4 Incubation buffer (Bottle 4)

100 ml
Ready-to-use solution, clear, colorless

Stability of Solution: see kit label imprint
For Use in: solution 9
Solution 5 Sample buffer (Bottle 5)

100 ml
Ready-to-use solution, clear or turbid, yellowish

Stability of Solution: see kit label imprint
For Use in: step 1
Solution 6 Washing buffer (Bottle 6)
Dissolve one tablet in 2 l of double-distilled water.
Result: A clear, colorless solution.
Stability of Solution: 12 months at 2 to 8 °C
For Use in: step 2
Solution 7 TMB substrate solution (Bottle 7)

15 ml
Ready-to-use solution, clear colorless to slightly yellowish.

Stability of Solution: see kit label imprint
For Use in: step 3
Solution 8 TMB stop solution (Bottle 8)

7 ml
Ready-to-use solution, clear, colorless

Stability of Solution: see kit label imprint
For Use in: step 4
Solution 9 Immunoreagent
For 100 wells (5 ml): Add 250 µl reconstituted anti-hEPO-HRP (solution 2) to 4.75 ml incubation buffer (solution 4), and mix well.
Stability of Solution: 8 hours at 2 to 8 °C. Warning: prepare shortly before use
For Use in: step 1
Storage conditions (working solution): Solution 1 hEPO control serum (bottle 1)
1 week at 2 to 8 °C
12 months at -15 to -25 °C
Solution 2 Anti-hEPO-HRP (bottle 2)
12 months at 2 to 8 °C
do not freeze!
Soluton 3 hEPO standard (bottles 3a-3f)
8 hours at 2 to 8 °C
aliquot the rest to be used for subsequent standard curves and store at -15 to -25 °C
Solution 4 Incubation buffer (bottle 4)
see kit label imprint
Solution 5 Sample buffer (bottle 5)
see kit label imprint
Solution 6 Washing buffer (bottle 6)
12 months at 2 to 8 °C
Solution 7 TMB substrate solution (bottle 7)
see kit label imprint
Solution 8 TMB stop solution (bottle 8)
see kit label imprint
Solution 9 Immuno-reagent
prepare shortly before use
8 hours at 2 to 8 °C

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 96 tests
Quality Level	100,
Торговая марка	Roche
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS09
signalword	Warning
hcodes	H290 - H315 - H317 - H319 - H411
pcodes	P261 - P264 - P273 - P280 - P333 + P313 - P391
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Erythropoietin, human (hEPO) 11120166001

Recombinant, Erythropoietin, human (hEPO), is produced in CHO cells (chinese hamster ovary) and purified by standard chromatographic techniques. The glycoprotein encoded by erythropoietin (EPO) is synthesized in kidneys. It is the principal hormone that has a short half-life. Cells responsive to EPO have been identified in adult bone marrow, fetal liver, or adult spleen.
Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specificity
Human Erythropoietin (hEPO) is effective on mouse and human cells.

Erythropoietin (EPO) is a glycoprotein which stimulates proliferation and differentiation of erythroid precursor cells (CFU-E, BFU-E) to more mature erythrocytes.
Biochem/physiol Actions
Erythropoietin (EPO) controls the synthesis of RBCs (red blood cells) by inducing the proliferation and differentiation of erythroid precursor cells. As it induces erythropoiesis, EPO can be used to treat anemia of cancer patients.
Sequence
One polypeptide chain (166 amino acids), glycosylated, identical to natural hEPO. The carbohydrate structure of recombinant EPO isolated from CHO cells is very similar to that of natural EPO.
Unit Definition
EC50 definition: The amount of hEPO that is required to produce equivalent [3H]-thymidine incorporation into spleen cells from phenylhydrazine-treated mice to that expressed by 1 unit of the WHO-EPO reference standard (2nd IRP) (1 unit equals 10 ng).
Physical form
Solution, filtered through 0.2 μm pore size membrane.
Preparation Note
Working solution: Dilute the concentrated EPO solution (250 U/ml) with PBS or culture medium containing 1 mg/ml (0.1%) BSA (or HSA) or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C
Note: Avoid repeated freezing and thawing

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in CHO cells Hamster
assay	>98% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 250 U (2.5 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<1 EU/µgtested (LAL test)
UniProt accession no.	P01588,
storage temp.	20°C
Gene Information	human ... EPO(2056)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

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Expand™ 20 kbPLUS PCR System, dNTPack 4743814001

The Expand 20 kbPLUS PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase, a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture and associated buffer system is designed to give a high yield of PCR product when fragments longer than 20 kb need to be amplified.
T/A cloning can also be performed.
The kit has control human genomic DNA and a set of control primers for amplifying a 23kb fragment. This test can be used to test template DNA and primer pair performance.
Use 3.75 U for a standard 50µL PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
To obtain long PCR products, first evaluate length and purity of the DNA template. Handling all genomic DNA templates with care. Genomic DNA template should be larger than 50kb, as determined with agarose gel electrophoresis.

Choose Expand™ 20 kbPLUS PCR System when amplifying genomic DNA fragments up to 35 kb. Expand 20 kbPLUS PCR System features a specifically optimized buffer and enzyme-blend mixture to amplify extra-long pieces of DNA (over 20 kb). Control primers and DNA produce fragments of 23 kb in length.

PCR
RT-PCR
Large-fragment amplification

Features and Benefits

Go the limit: The buffer and enzyme-blend amplify products over 20kb DNA.
Easy PCR monitoring: Human genomic DNA and human control -globin primers amplify a 23kb fragment.
Test template quality: Control reagents also test template quality and primer pair performance.
Cost-effective: Use the convenient premixed solution of PCR grade dNTPs.

Packaging
1 kit containing 7 components
Quality
Each lot of Expand 20kbPLUS PCR System is function-tested in PCR using human genomic DNA and specific primers for -globin to amplify a 23kb fragment.
Unit Definition
Volume Activity: 5 U/μl
Storage and Stability
Store supplied human control DNA at 2–8 °C, all other components at -15 to -25°C.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 40 reactions
feature	Long Range PCR, dNTPs included, hotstart: no
packaging	pkg of 200 U
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
parameter	68 °C optimum reaction temp.
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Expand™ High Fidelity PCR System

The 5′ → 3′ polymerase activity of Taq DNA Polymerase and the 3′ → 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′ → 5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. PCR up to 9 kb is possible, with yield diminishing as DNA fragment length increases. The Expand High Fidelity PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA polymerase. T/A cloning is recommended.

The Expand™ High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length in:

PCR
RT-PCR

Use Expand High Fidelity PCR System, dNTPack with ready-to-use PCR nucleotide mix.
For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.
Features and Benefits
Expand High Fidelity PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity for high yield and fidelity. It is optimized for PCR of DNA fragments up to 5 kb.
Use 2.6 U for a standard 50 µl PCR.
Volume activity: 3.5 U/µl

Use one enzyme for all applications.

Enjoy high yield, fidelity, and flexibility.

Detect PCR products

previously undetectable and avoid false negatives.

Achieve successful results

from small quantities of template DNA.
Packaging
1 kit containing 4 components
Quality
Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1 kb amplified product) and the tPA gene (4.8 kb amplified product).
Unit Definition
Volume Activity: 3.5 U/μl

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche

Quality Level	100,
usage	sufficient for 1,000 reactions (11759078001), sufficient for 200 reactions (11732650001), sufficient for 40 reactions (11732641001)
packaging	pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

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Expand™ Long Template PCR System

Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of PCR product from genomic DNA. Expand Long Template PCR System allows amplification of DNA fragments up to 20kb from human genomic DNA, and 40kb from DNA.
The Expand Long Template PCR System copies DNA threefold more accurately than Taq DNA Polymerase.
Misincorporations cause Taq DNA polymerase to fall off the DNA strand. The 3 5 exonuclease activity of the proofreading polymerase reaction mix amplify DNA three times more accurately than Taq DNA polymerase alone.
Long PCR products require a special buffer and optimized protocol. This kit has three buffers for amplifying fragments from 0.5 to 9 kb, 9 to 12 kb, and larger than 12kb. For high template quality, use Roches High Pure Template Preparation Kit to prepare genomic DNA template.

The main application of the Expand Long Template PCR System is the accurate amplification of very long target sequences, even from genomic DNA. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length.
Choose 1st generation Expand Long Template PCR System for long and accurate PCR reactions. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length. Use the product for:

PCR
RT-PCR or long-range PCR
Large-fragment amplification
Genome mapping and sequencing
Contig construction
Characterization of cloned sequences in lambda phages or cosmids
Rapid identification and cloning of complete genes from genomic DNA or cDNA sequence as starting material

As economical alternative we recommend to update to 2nd generation Expand Long Range, dNTPack.
For amplicon sizes of 20 to 35kb choose Expand 20kbPLUS PCR System, dNTPack.
Features and Benefits
Expand Long Template PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages or cosmids, and eukaryotic-gene cloning and analysis.

Amplify longer templates: This 3-buffer set amplifies products from 5 to 20kb.
Achieve higher yields and 3-fold higher fidelity compared to Taq DNA Polymerase.
Improve your PCR: Obtaining more full-length PCR product is ideal downstream.

Packaging
1 kit containing 4 components
Quality
Each lot is PCR function-tested using human genomic DNA and specific primers for a 9kb, 12kb, and 15kb fragment. The enzyme mix is tested for the absence of any contaminating activities, including endo- or exonucleases and nicking activity according to the current Quality Control procedures.
Unit Definition
Volume Activity: 5 U/μl
Preparation Note
Working concentration: Optimal enzyme concentration varies from 0.5 to 5.0 U per 50 µl reaction. The standard enzyme concentration is 3.75 U per 50 µl reaction.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 190 reactions (11681842001), sufficient for 38 reactions (11681834001), sufficient for 950 reactions (11759060001)
packaging	pkg of 3,600 U (11759060001 [10x360 U]), pkg of 150 U (11681834001), pkg of 720 U (11681842001 [2x360 U])
Торговая марка	Roche
parameter	68 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

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FastStart™ Taq DNA Polymerase, 5 U/l

FastStart™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart™ Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.

FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
It can be applied for:

PCR
Multiplex PCR
Difficult templates e.g., secondary structures or GC-rich sequences
Automated PCR e.g., handling at room temperatures
Hot Start PCR up to 3kb
Hot Start RT-PCR up to 3kb
Quantitative reverse transcription PCR (RT-qPCR)
Bisulfite-specific PCR

Use FastStart™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2x concentrated ready-to-use FastStart™ PCR Master.
Features and Benefits
FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations.

Higher specificity, sensitivity, and yield:

Hot start PCR makes PCR setup easier.

Use robotic setup.

Use this enyzme mix stable for 24hours at +15 to +25°C.

Prevent PCR carryover contamination.

Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes

optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates
superior results due to its unique enzyme design and optimized buffer system

Packaging
1 kit containing 5 components
Quality
Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.
Unit Definition
1 µg M13mp9ss DNA, 0.3 µg M13 sequencing primer and 0.1 µCi -32P dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Unit Assay: 1 µg M13mp9ss DNA, 0.3 µg M13 sequencing primer and 0.1 µCi -32P dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/µl
Preparation Note
Storage conditions (working solution): Fast Start Taq mastermix is stable for 4 weeks at -25 °C to -15 °C without primers
Please note: This parameter is not part of specification

For life science research only. Not for use in diagnostic procedures.

NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
FastStart is a trademark of Roche

sufficient for 1,250 reactions (12032945001), sufficient for 2,500 reactions (12032953001), sufficient for 250 reactions (12032929001), sufficient for 50 reactions (12032902001), sufficient for 500 reactions (12032937001)

packaging

pkg of 1,000 U (12032937001 4x250 U), pkg of 2,500 U (12032945001 10x250 U), pkg of 5,000 U (12032953001 20x250 U), pkg of 100 U (12032902001), pkg of 500 U (12032929001 2x250 U)

Fibronectin 10838039001

MDL number:

MFCD00131062

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Specificity
Active on most mammalian cells

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Testing Method
Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)
Physical form
Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.
Preparation Note
Working concentration: 5 µg/cm2
5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human plasma
sterility	non-sterile; 0.2 µm filtered
assay	95% (SDS-PAGE)
form	lyophilized (clear, colorless solution after reconstitution)
mol wt	440 kDa
packaging	pkg of 5 mg
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	P02751,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... FN1(2335)

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.
Sequence
Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.
Physical form
Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin
Preparation Note
Working concentration: 5µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Fibronectin (pure)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human plasma
sterility	non-sterile; 0.2 µm filtered
assay	>95% (SDS-PAGE)
form	lyophilized (clear, colorless solution after reconstitution)
mol wt	440 kDa
packaging	pkg of 1 mg (11051407001)","pkg of 5 mg (11080938001)
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	P02751,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... FN1(2335)

Formamide 11814320001

Empirical Formula (Hill Notation):	CH₃NO
Linear Formula:	HCONH₂
CAS Number:	75-12-7
Molecular Weight:	45.04
Beilstein/REAXYS Number:	505995
MDL number:	MFCD00007941
PubChem Substance ID:	329749563

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Formamide has been used in:
in situ hybridization

as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
as a component of stop buffer used in cleavage and polyadenylation activity assays

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Quality
Contaminants: 5 ppm heavy metals (as Pb), 5 ppm Fe

For life science research only. Not for use in diagnostic procedures.

vapor density	1.55 (vs air)
Quality Level	100,
vapor pressure	0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
assay	99%
form	liquid
autoignition temp.	932 °F
mol wt	45.04
packaging	pkg of 500 mL
Торговая марка	Roche
application(s)	hybridization: suitable
color	colorless
refractive index	n20/D 1.447 (lit.)
pH	4-10 (20 °C, 200 g/L)
bp	210 °C (lit.)
mp	2-3 °C (lit.)
density	1.134 g/mL at 25 °C (lit.)
absorption	0.08 at 290 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	NC=O
InChI	1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key	ZHNUHDYFZUAESO-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	305.6 °F
Flash Point C	152 °C

Formate Dehydrogenase

1.2.1.2 ( BRENDA | IUBMB )

Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Specificity
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Most widely used in cofactor recycling systems for NADH.
Quality
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Unit Definition
One unit (U) formate dehydrogenase will oxidize 1 µmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~0.4 units/mg protein (At 25 °C with formate as the substrate.)
packaging	pkg of 250 U (10837016001), pkg of 80 U (10244678001)
Торговая марка	Roche
optimum pH	7.5-8.5
shipped in	wet ice
storage temp.	2-8°C

O-2-Amino-2,7-dideoxy-δ-glycero-α-δ-glucoheptopyranosyl[1->4]-O-3-deoxy-4C-methyl-3-[methyl-amino]-β-L-arabinopyranosyl-δ-streptamine, disulfate salt G-418, an aminoglycoside antibiotic, is used as a dominant selection agent in transfection experiments. The antibiotic interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Transfection of neomycin-resistance genes into cells results in resistance to G-418 and enables the cells to grow in culture media containing G-418.

G-418 Solution

Empirical Formula (Hill Notation):	C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
CAS Number:	108321-42-2
Molecular Weight:	692.71
MDL number:	MFCD00058314
PubChem Substance ID:	329799925

G-418 Solution is used to select eukaryotic cells that are stably transfected with the neomycin resistance gene (neo), and to maintain the phenotype (neor) of resistant cells.
G-418 is also used to eliminate contaminating fibroblasts from mixed cultures.
Biochem/physiol Actions
Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.
Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Features and Benefits

Convenient sterile-filtered solution for immediate use – saves valuable time and effort, and avoids potential errors arising from the preparation of sterile working stock solution from G-418 powder.
Consistent quality and potency – shows no lot-to-lot variation in its ability to select resistant cells.
Function tested in a cell culture assay - ensures effectiveness.
Very pure, as indicated by TLC and microbiological assays – allows you to use less antibiotic while producing greater selection pressure. Lack of contaminants means minimal toxicity.
Cost effective, since the selected colonies are stable.

Contents
Aqueous solution of G-418, 892 µg/mg, filtered through 0.2µm pore size membrane
Quality
Purity: = 98%, as shown by TLC.
Function testing: in cell culture.
Preparation Note
Working concentration: The optimal concentration of G-418 must be determined experimentally and varies with the cell type used.
Active weight: >700µg/mg
Recommended concentration in cell culture: The optimal concentration of G-418 must be determined experimentally; it varies from 100µg/ml to 1mg/ml, depending on the cell type used.
Specific rotation: 104° to 121°, in water (on dry basis, according to USP XXII).

For life science research only. Not for use in diagnostic procedures.

assay	98% (TLC)
Quality Level	100,
form	solution
mol wt	692.7 Da
packaging	pkg of 100 ml (04727894001 [5 x 20 ml, equivalent to 5 g]), pkg of 20 mL (04727878001 [equivalent to 1 g])
Торговая марка	Roche
concentration	50 mg/mL
application(s)	transfection: suitable
Mode of action	protein synthesis \| inhibits
antibiotic activity spectrum	protozoa
shipped in	wet ice
storage temp.	2-8°C
SMILES string	OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI	1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;21-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key	UHEPSJJJMTWUCP-NKCAIAFTSA-N

GC-RICH PCR System, dNTPack 4743784001

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.
Features and Benefits

Ease access to difficult templates:-

including GC-rich targets and repetitive sequences.

Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
Amplify DNA fragments up to 5 kb.
Cost-effective:-

use the convenient premixed solution of PCR grade NTPs.
Packaging
1 kit containing 6 components
Quality
The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.
Unit Definition
Volume Activity: 2 U/μl
Preparation Note
Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 µl PCR, we recommend using 2 U of the enzyme blend.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤50 reactions
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart: no
packaging	pkg of 100 U
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	−20°C (−15°C to −25°C)

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

Genopure Buffer Set 4634772001

Doubling the volume of the Suspension, Lysis, and Neutralization Buffers during the Genopure plasmid preparation procedure will provide better yields of plasmid DNA from low-copy number plasmids. The buffers in this set may be used with either of the Genopure Plasmid Kits (Midi or Maxi).
Features and Benefits
The Genopure Buffer Set for Low-Copy Number Plasmids provides additional buffers to supplement those in the Genopure Plasmid Kits.
RNase A is also included in the set because it eliminates bacterial RNA from the preparation, thereby improving the recovery of low copy number plasmids.

Convenient, ready-to-use, function-tested, nuclease-free buffers
Helps the Genopure Plasmid Midi and Maxi Kits enhance plasmid yield
Ensures reproducible results

Components

Suspension Buffer, 500 mL
RNase A, 50 mg
Lysis Buffer, 500 mL
Neutralization Buffer, 500 mL

For life science research only. Not for use in diagnostic procedures.

To learn how these buffers are used, see the product listings or the package inserts for the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 20 maxi preps or 60 midi preps for low copy number plasmids

pictograms	GHS05
signalword	Danger
hcodes	H290 - H314
pcodes	P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P390
RIDADR	UN 1824 8 / PGIII
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Genopure Plasmid Maxi Kit 3143422001

For large-scale (maxi) preparation of plasmid DNA.

The Genopure Plasmid Maxi Kit prepares transfection-grade plasmid DNA in large quantities (up to 500 µg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:

Transfection
Southern blotting
Sequencing
PCR/long PCR
Restriction digestion
Cloning

Features and Benefits
The Genopure Plasmid Maxi Kit prepares highly purified plasmid DNA in large quantities using a modified alkaline lysis method.

Save time with ready-to-use reagents.

Purify up to 10 samples (10 minutes hands-on-time/75 minutes overall).

Purify all sizes and types of plasmid

even BAC DNA, since the crude lysate can be filtered to avoid plasmid shearing.

Process multiple samples in parallel

using high speed gravity-flow columns.

Eliminate the use of hazardous organic compounds

such as cesium chloride, phenol, chloroform, and ethidium bromide.

Obtain higher purity plasmid DNA

over plasmid prepared by 2 x cesium chloride gradient centrifugation.
Components

Suspension Buffer
RNase A
Lysis Buffer
Neutralization Buffer
Equilibration Buffer
Wash Buffer
Elution Buffer
NucleoBond AX 500 Columns
Folded Filters (240 mm diameter)
Sealing Rings

Quality
Plasmid DNA purified by this kit has been tested for restriction digestion; pUC 19 was isolated from transformed HB101 as described in the protocol. 1 µg of plasmid was completely digested with 1 U Msp I for 2 hours at +37°C, as shown by agarose gel analysis.
Plasmid recovery was tested with 250 µg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 150 ml culture volume with a density of A600 between 3 and 6, >400 µg of plasmid DNA was obtained.
The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.
RNA contamination was analyzed with 3 µg pBS purified with the standard procedure and checked by electrophoresis on an agarose gel. No RNA was detected.
The kit components have been tested for the absence of nucleases according to current quality control procedures.
Preparation Note
The isolation procedure is based on a modified alkaline lysis protocol and can be divided into the following steps:The bacteria are partially lysed, allowing the plasmid DNA to escape the cell wall into the supernatant. The larger E. coli chromosomal DNA is trapped in the cell wall. The lysate is cleared of cellular debris by filtration or centrifugation, and the plasmid DNA-containing fraction is loaded onto a pre-equilibrated column. Since the cleared lysate is applied to the column in a low-salt buffer, plasmid DNA binds to the macroporous anion-exchange material in the column. Cellular impurities are eluted from the column with a high-salt wash. Finally, the plasmid DNA is eluted from the column. Recovered DNA is precipitated from the eluate to remove salt and concentrate the plasmid.
Analysis Note
Sample:
E. coli culture that contains a high-copy number plasmid: 30 to 150 mL bacterial culture
E. coli culture that contains a low-copy number plasmid: 100 to 500 mL bacterial culture
Plasmid Size: The isolation procedure is suitable for all sizes of plasmid. Note: Lysates of larger constructs (up to 100 kb) should be cleared by filtration rather than centrifugation to avoid shearing the plasmid.
Time Required: 75 minutes (including filtration of the lysate)
Typical Yield:
High-copy number plasmid: 3 to 5 µg/mL culture
Low-copy number plasmid: 0.2 to 1 µg/mL culture
Product Purity: Isolated plasmid DNA is free of other bacterial components, including RNA.

Ion Exchange Chromatography,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 10 isolations from 30 to 150 ml

pictograms	GHS02,GHS05
signalword	Danger
hcodes	H226 - H290 - H314
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
RIDADR	UN 3316 9
WGK Germany	WGK 1
Flash Point F	100.4 °F
Flash Point C	38 °C

Genopure Plasmid Midi Kit 3143414001

Plasmid recovery was tested with 50 µg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 mL culture volume with a density of A600 between 3 and 6, >85 µg of plasmid DNA was obtained.
The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.
No RNA is detectable.
The kit components have been tested for the absence of nucleases according to current quality control procedures.

The Genopure Plasmid Midi Kit prepares transfection-grade plasmid DNA in medium quantities (up to 100 µg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:

Transfection
Southern blotting
Sequencing
PCR
Restriction analysis
Cloning

Features and Benefits
The Genopure Plasmid Midi Kit prepares highly purified plasmid DNA in medium quantities using a modified alkaline lysis method.

Save time with ready-to-use reagents.

Purify up to 20 samples (10 minutes hands-on-time/75 minutes overall).

Purify all sizes and types of plasmid,

even BAC DNA, since the crude lysate can be filtered to avoid plasmid shearing.

Process multiple samples in parallel

using high speed gravity-flow columns.

Eliminate the use of hazardous organic compounds

such as cesium chloride, phenol, chloroform, and ethidium bromide.

Obtain higher purity plasmid DNA

over plasmid prepared by 2 x cesium chloride gradient centrifugation.
Components

Suspension Buffer
RNase A
Lysis Buffer
Neutralization Buffer
Equilibration Buffer
Wash Buffer
Elution Buffer,
NucleoBond AX 100 Columns
Folded Filters (150 mm diameter)
Sealing Rings

Quality
Plasmid DNA purified by this kit has been tested for restriction digestion; pUC 19 was isolated from transformed HB101 as described in the protocol. 1 µg of plasmid was completely digested with 1 U Msp I for 2 hours at +37°C, as shown by agarose gel analysis.
Plasmid recovery was tested with 50 µg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 ml culture volume with a density of A600 between 3 and 6, >85 µg of plasmid DNA was obtained.
The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.
RNA contamination was analyzed with 3 µg pBS purified with the standard procedure and checked by electrophoresis on an agarose gel. No RNA was detected.
The kit components have been tested for the absence of nucleases according to current Quality Control procedures.
Preparation Note
The isolation procedure is based on a modified alkaline lysis protocol and can be divided into the following steps:The bacteria are partially lysed, allowing the plasmid DNA to escape the cell wall into the supernatant. The larger E. coli chromosomal DNA is trapped in the cell wall. The lysate is cleared of cellular debris by filtration or centrifugation, and the plasmid DNA-containing fraction is loaded onto a pre-equilibrated column. Since the cleared lysate is applied to the column in a low-salt buffer, plasmid DNA binds to the macroporous anion-exchange material in the column. Cellular impurities are eluted from the column with a high-salt wash. Finally, the plasmid DNA is eluted from the column. Recovered DNA is precipitated from the eluate to remove salt and concentrate the plasmid.
Analysis Note
Sample:
E. coli culture that contains a high-copy number plasmid: 5 to 30 mL bacterial culture
E. coli culture that contains a low-copy number plasmid: 10 to 100 mL bacterial culture
Plasmid Size: The isolation procedure is suitable for all sizes of plasmid. Note: Lysates of larger constructs (up to 100 kb) should be cleared by filtration rather than centrifugation to avoid shearing the plasmid.
Time Required: 60 minutes (including filtration of the lysate)
Typical Yield:
High-copy number plasmid: 3 to 5 µg/mL culture
Low-copy number plasmid: 0.2 to 1 µg/mL culture
Product Purity: Isolated plasmid DNA is free of other bacterial components, including RNA.

Ion Exchange Chromatography,
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 20 isolations from 5 to 30 ml

pictograms	GHS02,GHS05
signalword	Danger
hcodes	H226 - H290 - H314
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
RIDADR	UN 3316 9
WGK Germany	WGK 1
Flash Point F	100.4 °F
Flash Point C	38 °C

Glucose-6-phosphate 10127647001

Empirical Formula (Hill Notation):	C₆H₁₁Na₂O₉P · xH₂O
CAS Number:	3671-99-6
Molecular Weight:	304.10 (anhydrous basis)
Beilstein/REAXYS Number:	5199009
MDL number:	MFCD00150940
PubChem Substance ID:	329748950

Disodium salt

Substrate for glucose-6-phosphatase.
Biochem/physiol Actions
In mammals, glucose-6-phosphate (G6P) is formed by a kinase acting on glucose. It plays several roles and has its fate determined accordingly. It can get converted back to glucose by a phosphatase enzyme. It can pass into glycogen, or enter into the energy-yielding Embden-Meyerhof path or into the 6-phosphogluconate pathway. Increased levels of blood glucose result in elevated levels of glucose 6-phosphate in liver, skeletal muscle, and adipose tissue. This elevated intracellular level of G6P activates glycogen synthase. G6P may also be involved in the negative regulation of phosphorylation of glycogen synthase via cyclic AMP-stimulated protein kinase.
Components
77% glucose-6-P (enzymatic), 13% sodium, 8% water
Quality
Contaminants: <0.2% glucose (enzymatic)
Formula variant
C6H11O9PNa2

For life science research only. Not for use in diagnostic procedures.

mol wt	(glucose-6-P: Mr = 260.2, glucose-6-P-Na2: Mr = 304.2)
packaging	pkg of 5 g
Торговая марка	Roche
shipped in	ambient
SMILES string	O.[Na+].[Na+].OC1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H](O)[C@H]1O
InChI	1S/C6H13O9P.2Na.H2O/c7-3-2(1-14-16(11,12)13)15-6(10)5(9)4(3)8;;;/h2-10H,1H2,(H2,11,12,13);;;1H2/q;2*+1;/p-2/t2-,3-,4+,5-,6?;;;/m1.../s1
InChI key	UUWJZXLTPORJKW-WYFATIGWSA-L

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Glucose-6-Phosphate Dehydrogenase (G6P-DH) 10165875001

1.1.1.49 ( BRENDA | IUBMB )

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1
Specificity
Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (Km = 36 µM, NADP as coenzyme; 64 µM, NAD as coenzyme), but will use either NADP (Km = 7.4 µM; relative rate 1.0) or NAD (Km = 115 µM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Component of cofactor recycling systems for NADPH.
It was used in enzymatic determination of C6 phosphorylation of glycogen.
Quality
Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH
Unit Definition
Unit Conversion: One unit (U) [+25 °C] 1.18 U [+30 °C] 1.45 U [+37 °C], all with NAD as coenzyme
Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.
Physical form
Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6
Preparation Note
Activator: HCO3- ( 0.3 M) activates slightly.
Analysis Note
Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution, suspension
specific activity	~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
packaging	pkg of 1 mL (1,000 U)
Торговая марка	Roche
optimum pH	7.0-8.5(maximal activity at 7.8)
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Glucose-6-Phosphate Dehydrogenase (G6P-DH) 10127655001

1.1.1.49 ( BRENDA | IUBMB )

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP+ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP+ and NADPH levels the equilibrium shifts to inactive tetramers.

Component of cofactor recycling systems for NADPH.
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP+ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.
Specifications
Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI
Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: Mg2+ (5 to 10 mM)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
packaging	pkg of 1 mL (5 mg/ml)
Торговая марка	Roche
optimum pH	9.2
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Glutamate Dehydrogenase (GlDH) 10197734001

1.4.1.3 ( BRENDA | IUBMB )

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination.

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.
Quality
Contaminants: <0.005% ADH, LDH, and MDH, each
Sequence
The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.), ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
packaging	pkg of 3,000 U
Торговая марка	Roche
optimum pH	8
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Glutamate-Oxaloacetate Transaminase (GOT)

2.6.1.1 ( BRENDA | IUBMB )

Glutamate-Oxaloacetate Transaminase (GOT) is a transaminase enzyme which catalyzes amino group of an amino acid with keto group of keto acid.
L-aspartate:2-oxoglutarate aminotransferase

Synthesis of unnatural L-amino acids from α-keto acids.
Unit Definition
Unit Conversion: One unit (+25 °C, pH 7.4) ≈ 1.9 units (+37 °C, pH 7.4)
Physical form
Suspension in 3.2 M ammonium sulfate solution, 2.5 mM 2-oxoglutarate, 50 mM maleate, and 1 mM pyridoxal phosphate, pH approximately 6
Preparation Note
Activator: Pyridoxal phosphate, pyridoxamine phosphate

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~200 units/mg protein (At 25 °C (380 U/mg at 37 °C) with L-aspartate and 2-oxoglutarate as the substrates)
packaging	pkg of 1 mL (10105546001 [2 mg]), pkg of 1 mL (10105554001 [10 mg]), pkg of 2.5 mL (10737046001 [25 mg])
Торговая марка	Roche
optimum pH	6.5-8.5
shipped in	wet ice
storage temp.	2-8°C

Glutamate-Pyruvate Transaminase (GPT)

2.6.1.2 ( BRENDA | IUBMB )

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Synthesis of unnatural L-amino acids from α-keto acids.
Quality
Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT
Unit Definition
Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
packaging	pkg of 1 mL (10105589001 [10 mg]), pkg of 2.5 mL (10737127001 [25 mg])
Торговая марка	Roche
optimum pH	~8.0
shipped in	wet ice
storage temp.	2-8°C

Glycogen 10901393001

This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs.
20 µg glycogen (1 µl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 µl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1µl glycogen solution (20 µg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 x g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.
Features and Benefits
Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.

Contents
Aqueous solution, 20 mg/ml
Quality
Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1 mL (20 mg)
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

GTP--S 10220647001

Guanosine 5-O-(3-thiotriphosphate), tetralithium salt (GTP--S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Biochem/physiol Actions
Guanosine 5-O-(3-thiotriphosphate) (GTP--S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),
Storage and Stability
Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.
Analysis Note
Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:

GTP--S has been used:

in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (G)
to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase C2 mutants

approx. 10 mg/ml double-dist. water
dilution: 9.8 ml double-dist. water + 0.2 ml sample.

For life science research only. Not for use in diagnostic procedures.

description	C₁₀H₁₂N₅O₁₃P₃SLi₄
Quality Level	100,
assay	1% (GMP, HPLC), 12% (GDP, HPLC), 2% (GTP, HPLC)
mol wt	M_r 539.2 (GTP--S), M_r 563.0 (GTP--S-Li₄)
packaging	pkg of 10 mg
Торговая марка	Roche
storage condition	protect from light
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash