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Antipain dihydrochloride 11004646001
Empirical Formula (Hill Notation): C27H44N10O6 · 2 HCl CAS Number: 37682-72-7 Molecular Weight: 677.62 MDL number: MFCD00135957 PubChem Substance ID: 329749271 General descriptionAntipain is a protease inhibitor isolated from actinomycetes. It inhibits thrombokinase and blood coagulation.ApplicationConcentrations for 50% inhibition (µg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 µM
Biochem/physiol Actions
Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.
Formula variant
Formula: C27H44N10O6 x 2 HCl
Preparation Note
Working concentration: 50 µg/ml
Working solution: Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl3), hexane, or petroleum and ethyl ethers.
Storage conditions (working solution): -15 to -25 °C
Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Growth of microorganisms and repeated freezing should be avoided. Stable for approximately one month.
Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.8 A stock solution in water or buffer is stable for about a month at -20 °C.
Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Reconstitution
Soluble in water, methanol, or DMSO to 20 mg/ml. Sparingly soluble in ethanol, propanol, or butanol. Insoluble in benzene, chloroform (CHCl3), hexane, or petroleum and ethyl ethers. Dilute solutions should be stored frozen in aliquots at -15 to -25 °C. Stable for approximately one month.
CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform crystalline Quality Level 100, mol wt Mr = 677.63 packaging pkg of 10 mg Торговая марка Roche mp 209-217 °C solubility DMSO: 20 mg/mL, methanol: 20 mg/mL, water: 20 mg/mL, benzene: insoluble, chloroform: insoluble, ethanol: sparingly soluble, hexane: insoluble shipped in wet ice storage temp. 2-8°C SMILES string Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C InChI 1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1 InChI key YAHXZYICKJUJEO-BXLPLHKWSA-N
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Apoptotic DNA-Ladder Kit 11835246001
General descriptionThis kit provides a way to isolate apoptotic DNA fragments for DNA ladder analysis. The purification method used is much faster than other DNA purification methods (e.g., phenol/chloroform extraction, DNA precipitation). Purified DNA can be mixed directly with gel loading buffer and analyzed on an agarose gel.
The study of cell death involves characterizing mortality as apoptotic or necrotic. Apoptosis can be characterized by:- Prelytic, non-random fragmentation of DNA (“ladder” pattern after agarose-gel electrophoresis)1
- Formation of membrane-bound vesicles (or “apoptotic bodies”)
- Cell shrinkage due to a concentration of cytoplasm
Similarly, necrosis (or physiological cell death) is characterized by- Random digestion of DNA (DNA smear after agarose-gel electrophoresis)
- Swollen organelles and cells, resulting from loss of membrane integrity and cell lysis
- Postlytic DNA fragmentation
As these differences indicate, the classification of cell death can be accomplished through observing cell morphology, or less subjectively, by analyzing genomic DNA. Resolving the DNA on a gel provides a quick and documentable form of data to differentiate between apoptosis and necrosis.
Specificity
Only nucleic acid will bind to the glass fiber filters under the conditions outlined in the kit. Salts, proteins, and other cellular components do not bind.ApplicationThe Apoptotic DNA-Ladder Kit provides rapid isolation of DNA, which can be analyzed and characterized by gel electrophoresis for the determination of apoptotic cell death.
Packaging
1 kit containing 6 components.
Principle
Blood or cell lysis is accomplished by incubating the sample with the special Binding/Lysis Buffer. The sample is centrifuged through a column that contains glass fiber fleece. In the Binding/Lysis Buffer, nucleic acids quickly and preferentially bind to the surface of the glass fibers. The Washing Buffer rinses away salts, proteins, and other cellular debris. DNA is subsequently collected via a centrifugation step with elution buffer.
Preparation Note
Working solution: Note: Before starting a purification reaction warm the Elution Buffer to 70 °C, all other reagents should be at 15 to 25 °C.
Preparation of Working Solutions- Add 80 ml ethanol, analysis grade, to Washing Buffer
- Dissolve Positive Control (violet cap) in 400 µl Binding/Lysis Buffer and mix immediately.
- Incubate for 10 minutes at 15 to 25 °C.
EDTA solution (0.5 M)
Dissolve 18.6 g EDTA in 80 ml double dist. water and stir. Adjust pH 8.0 ± 0.1 with 1 M NaOH. EDTA solubilizes at alkaline pH only. After solubilization fill up to 100 ml with double dist. water.
TBE-Buffer
Dissolve 5.4 g Tris, 2.8 g Boric acid in 800 ml double dist. water and add 2 ml
of 0.5 M EDTA solution . Stir until dissolved, final pH 8.0 ± 0.1. Fill up to 1 liter with double dist. water.
Ethidium bromide stock solution
Dissolve 50 mg ethidium bromide in 5 ml double dist. water (ethidium bromide is a mutagen and potential carcinogen; gloves should be worn and care should be taken when handling ethidium bromide solutions).
Alternatively SYBR Green I Nucleic Acid Gel Stain can be used instead of Ethidium bromide.
Loading Buffer (10x)
Dissolve 0.1 g sodium dodecyl sulfate, 25 mg Bromophenol Blue in 7 ml double dist. water and add 3 ml glycerol
Storage conditions (working solution): The positive control can be used for 14 days after preparation, when storing the isolated control DNA at -15 to -25 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationNOTICE TO PURCHASER: This is a product licensed under patents owned by Qiagen.Параметрыusage sufficient for ≤20 tests Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 20-25°C
Safety Informationpictograms GHS05,GHS07 signalword Danger hcodes H302 - H315 - H318 - H412 pcodes P264 - P270 - P273 - P280 - P305 + P351 + P338 + P310 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F does not flash Flash Point C does not flash Узнать цену -
Aprotinin
Кат. номер 10236624001 11583794001 10981532001 General descriptionTrypsin inhibitor, pancreas type from bovine lung. It is known as Pancreatic trypsin inhibitor (BPTI). Aprotinin, also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhibitor, is found to be expressed in lungs, spleen, liver, and pancreas. It is also found to be present in the free form in calf serum.
Specificity
Aprotinin inhibits serine proteases. It inhibits kallikrein, the protease that releases hypotensive peptides such as kallidin and bradykinin (human plasma kallikrein: Ki = 3 x10-8 M at pH 8.0, porcine pancreas kallikrein: Ki = 1 x 10-9 M at pH 8.0), trypsin (Ki = 2.8 x 10-11 M at pH 7.8, Ki = 2.6 x 10-9 M at pH 4.0, non-competetive), trypsinogen, chymotrypsin (Ki = 9 x 10-9 M at pH 8.0), bacterial fibrinolysin, and plasmin (Ki = 1 nM at pH 7.3).
Cathepsin G, acrosin, human leukocyte elastase, and human urokinase are weakly inhibited. Factor Xa, thrombin, subtilisin, papain, pepsin, angiotensin-converting enzyme (ACE), carboxypeptidase A and B, other metalloproteases, and thiolproteases are not inhibited.ApplicationAprotinin is used for the protection of proteins and enzymes during isolation/purification. The inhibition of protease activity increases the lifetime of cells in cell and tissue culture studies.- Further applications: Purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin
- Quantification of kallikrein activity in mixtures of esterases and proteases
- Controlled degradation of substrates by avoiding nonspecific proteolysis in clinical chemical tests
- Aprotinin as a model protein in protein-folding studies
- Molecular weight marker in SDS-polyacrylamide gel electrophoresis
Sequence
Monomeric peptide of 58 amino acids held in conformation by three disulfide bonds.
Unit Definition
One inhibitor unit (IU) is defined as the amount of aprotinin that completely inhibits 1 U trypsin in < 10 minutes at pH 6. (Trypsin activity determined at +25 °C, pH 8.0, BAEE as substrate).
One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 2.8 inhibitor units (+25 °C, Chromozym TRY as substrate).
One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 26 kallikrein inhibitor units (KIU) (+25 °C).
One inhibitor unit (IU) (+25 °C, BAEE as substrate) corresponds to about 0.067 inhibitor units (+25 °C; Bz-D,L-Arg-4-Na as substrate, trypsin determination at pH 7.8).
One kallikrein inhibitor unit = 0.17 µg crystalline aprotinin.
Preparation Note
Working concentration: 0.06 to 2 µg/ml (0.01 - 0.3 µM)
Working solution: Soluble in water (10 mg/ml) or aqueous buffer solution (e.g., 0.1 M Tris, pH 8.0).
Note: To avoid adsorption of aprotinin onto negatively charged solid phases, e.g., chromatography gels, ultrafiltration membranes, the NaCl concentration should be above 0.1 M or other suitable salts should be added to all buffers used during the separation.
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Freely soluble in water (10 mg/ml) or aqueous buffer solution (e.g., Tris, 0.1 M, pH 8.0). A solution adjusted to pH 7 to 8 is stable for approximately 1 week at 2 to 8 °C.
Aliquots stored at -15 to -25 °C are stable for approximately 6 months.
Note: Avoid repeated freezing and thawing and exposure to strongly alkaline solutions (inactive at pH > 12.8).Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine lung form lyophilized packaging pkg of 10 mg (10236624001), pkg of 100 mg (11583794001), pkg of 50 mg (10981532001) Торговая марка Roche pH range 3 - 10 solubility water: soluble 10 mg/mL absorption 0.84 at 280 nm shipped in wet ice storage temp. 2-8°C
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Ascorbate Oxidase Spatula 10736619001
Enzyme Commission (EC) Number: 1.10.3.3 ( BRENDA | IUBMB ) General descriptionL-ascorbate:oxygen oxidoreductase
Specificity
Specific activity: One spatula contains approximately 17 U ascorbate oxidase at +25°C with L-ascorbate as the substrate.ApplicationThe spatula serves to remove ascorbic acid from aqueous solutions in which the presence of ascorbic acid (ascorbate, vitamin C) can interfere with the investigation of the sample material, for example, the detection of blood (erythrocytes) in urine or the determination of oxalic acid in urine during research studies.
The ascorbic acid to be removed should not exceed a concentration of 10 µg/ml. For concentrations in the range of 50 to 100 µg/ml, stir for 15 minutes. For higher concentrations, the solution should be appropriately diluted.
Physical form
Spatulas
Storage and Stability
Store at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, packaging pkg of 25 ea Торговая марка Roche storage condition protect from light optimum pH ~6.0 pH range 5.6 - 7.0 shipped in wet ice
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ATP Bioluminescence Assay Kit CLS II 11699695001
General descriptionATP bioluminescence assay is a sophisticated micro count method. It works based on the capability of fireflies to generate its glowing cold light. Rise in proliferation for all cytohexdrin concentrations can be determined by ATP bioluminescence assay.
Specificity
The ATP Bioluminescence Assay Kit CLS II is specially developed for applications in which constant light signals are required for kinetic studies of enzymes and metabolic studies, or if coupled enzymatic assays are applied. If ATP determinations are performed manually, the CLS Kit provides high reproducibility due to the constant signal generation.
The sensitivity of the kit is reduced by a factor of 10, compared to the ATP Bioluminescence Assay Kit HS II, which is recommended for determinations that are in the high-sensitivity range. The ATP Bioluminescence Assay Kit HS II also contains an efficient cell lysis reagent, and can be used for the detection of ATP in microorganisms or animal cells.ApplicationATP Bioluminescence Assay Kit CLS II has been used in the determination of the ATP synthetic activity of the H+-ATP synthase, ATP bioluminescent assay and intracellular measurement of ATP levels.
The ATP Bioluminescence Assay Kit CLS II is optimized for easy use in tube luminometers and microplate-format luminometers. The kit exhibits a constant light signal that is sustained for several minutes. The kit is well suited for kinetic studies and ATP determinations in coupled enzymatic reactions.
For more highly sensitive ATP determinations, use the ATP Bioluminescence Assay Kit HS II.
Packaging
1 kit containing 2 components.
Preparation Note
Working concentration: Working range: 10-6 to 10-11 M ATP
Working solution: Preparation of working solutions
Luciferase Reagent
Dissolve the whole content of one bottle by carefully adding 10 ml of double-dist. water. Incubate for five minutes at 0 to 4 °C without stirring or shaking. Mix for a homogeneous solution by carefully rotating the bottle. Do not shake. The reagent is stable for one day at 15 to 25 °C or for one week when stored at 0 to 4 °C. However, set up a standard curve each day, because a slightly loss of light activity occurs during this time (approx. 20% after 5 days).
ATP Standard
Each bottle contains approx. 10 mg ATP (> 98% purity; Mr 605.2). The exact amount of ATP is determined individually for each lot as indicated on the label. Dissolve the content of one bottle by addition of the appropriate volume of double-dist. water to get a final concentration of 10 mg/ml or 16.5 mM, respectively (e.g., 960 µl to 9.60 mg ATP).
Reconstitution
Roche recommends reconstituting Luciferase Reagent in water and not in buffer.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay >98% Quality Level 100, usage sufficient for 1,600 assays (microplate), sufficient for 800 assays (tubes) Торговая марка Roche parameter 15-25 °C optimum reaction temp. shipped in dry ice storage temp. −20°C
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ATP Bioluminescence Assay Kit HS II 11699709001
General descriptionLiving things require a continual input of free energy for three major purposes: the performance of mechanical work in muscle contraction and other cellular movements, the active transport of molecules and ions, and the synthesis of macromolecules and other biomolecules from simple precursors.
The free energy used in these processes is derived from the surroundings and maintains an organism in a state that is far from equilibrium. In most processes, this special carrier of free energy is adenosine triphosphate (ATP), an energy-rich molecule. Its triphosphate unit contains two phosphoanhydride bonds. The turnover of ATP is very high. Motion, active transport, signal amplification, and biosynthesis (as is needed for cell proliferation) can occur only if ATP is continuously regenerated from ADP. Therefore, measurement of ATP can serve as a marker for cell proliferation.
The determination of ATP using bioluminescence is a well established technique. It uses the ATP dependency of the light-emitting, luciferase-catalyzed oxidation of luciferin for the measurement of extremely low concentrations of ATP.
Specificity
The ATP Bioluminescence Assay Kit HS II is specifically developed for the high-sensitivity detection of ATP. Due to the high concentration of luciferase in the assay, the reaction exhibits a peak kinetic. If ATP determinations are performed to obtain kinetic data of the enzymes involved (e.g., for metabolic studies or if coupled enzymatic assays are applied), the use of the ATP Bioluminescence Assay Kit CLS II (generating a constant light signal) is highly recommended.ApplicationHighly sensitive and quantitative determination of ATP. Contains a cell lysis reagent and can be applied for the detection of microbial contamination. The kit is well suited for use in tube luminometers and microplate-format luminometers. The cell lysis reagent exhibits good lysis efficiency with respect to a variety of eukaryotic and prokaryotic cells.
For a time constant light signal, use the ATP Bioluminescence Assay Kit CLS II.
For maximum sensitivity, the sample ATP must be in a minimum volume, and the luciferase reagent must not be diluted.
Biochem/physiol Actions
The luciferase from Photinus pyralis (American firefly) catalyzes the following reaction:
ATP + D-luciferin + O2? oxyluciferin + PPi + AMP + CO2 + light.
The quantum yield for this reaction is about 90%. The resulting green light has an emission maximum at 562 nm.
The Michaelis equation has the following form:
light intensity = (Vmax x CATP)/(Km + CATP).
At low ATP concentrations (CATPm), the formula is simplified to light intensity = Vmax x CATP/Km.
From this equation, it becomes obvious that the light output is directly proportional to the ATP concentration (CATP), and is dependent on the amount of luciferase (Vmax) present in the assay.
Packaging
1 kit containing 4 components.
Preparation Note
Working concentration: 10-5 to 10-12 M ATP
Working solution: Preparation of Working Solutions
Luciferase Reagent
Dissolve the whole content of one vial 1 by carefully adding 10 ml of dilution buffer from vial 4. Incubate for 5 minutes at 0 to 4 °C without stirring or shaking. Mix for a homogeneous solution by carefully rotating the bottle. Do not shake!
Stability: Set up a standard curve each day, because a slight loss of luciferase activity may occur during this time (approx. 20% after storage for 5 days at 2 to 8 °C).
ATP Standard
Information Note: Each bottle contains approx. 10 mg ATP (> 98% purity; Mr 605.2). The exact amount of ATP is determined individually for each lot as indicated on the label.
Dissolve the content of one vial 2 by addition of the appropriate volume of dilution buffer to get a final concentration of 10 mg/ml or 16.5 mM, respectively (e.g., 960 µl to 9.60 mg ATP).
The ATP standard curve is prepared by serial dilutions of one ATP standard with dilution buffer.
Cell Lysis Reagent
The cell lysis reagent is ready-to-use.
Important Note: Avoid contamination by microorganisms or ATP. The use of autoclaved or heat sterilized labware is recommended.
Dilution Buffer
The dilution buffer supplied with the kit is essentially free of ATP and microbial contaminations. Use this buffer for reconstitution and dilution of the kit reagents. It can also be used as diluent for samples.
Important Note: Avoid contamination and use autoclaved or heat sterilized labware.
Storage conditions (working solution): Luciferase Reagent
The reagent is stable for one day at 15 to 25 °C or for one week when stored at 0 to 4 °C.
Reconstituted Luciferase Reagent may be stored frozen at -15 to -25 °C for longer periods of time.
Important Note: Each freeze/thaw-cycle reduces luciferase activity, depending on the freezing conditions (shock freezing is most effective). For best results, avoid repeated freezing and thawing.
ATP Standard
When stored at 2 to 8 °C, the solution is stable for one week (< 5% degradation).
When stored at 15 to 25 °C, the solution is stable for at least 4 weeks (< 5% degradation).
When stored on ice, diluted ATP-standards are stable for 8 hours.
Cell Lysis Reagent
The reagent is stable at 2 to 8 °C.
Dilution Buffer
Store at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay >98% Quality Level 100, usage sufficient for 1,000 assays (microplate), sufficient for 500 assays (tubes) Торговая марка Roche shipped in dry ice storage temp. −20°C
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ATP--S 11162306001
General descriptionAdenosine 5′-O-(3-thiotriphosphate), lithium salt, solution.ApplicationATP-γ-S is a substrate and inhibitor of ATP-dependent enzyme systems. It is hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases.
Quality
Contaminants: 10% ADP (HPLC)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, description C10H16N5O12P3SLi (Formula) assay 90% (HPLC) form solution mol wt 523.2 packaging pkg of 200 µL (20 µmol) Торговая марка Roche max 260 nm at 100 mmol/L shipped in dry ice storage temp. 20°C
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Basic Fibroblast Growth Factor, human (hbFGF)
Кат. номер 11123149001 11120417001 General descriptionBovine bFGF has 146 amino acids. bFGF shares 55% amino acid homology with a second endothelial cell mitogen, acidic FGF (aFGF) (also known as endothelial cell growth factor, ECGF). The amino acid sequence for human bFGF is very similar to the sequence of the bovine protein. The proteins differ only by two amino acids. Both the human and bovine proteins appear to be synthesized initially with an amino-terminal extension of nine residues. bFGF is a potent mitogen for a wide variety of mesoderm- and neuroectoderm- derived cells including fibroblasts, vascular and corneal endothelial cells, myoblasts, chondrocytes, osteoblasts, smooth muscle cells, and glial cells. All cell types that respond to FGFs bear specific FGF cell surface receptors. In addition, bFGF induces blood vessel growth and wound healing in vivo. Other in vivo effects of bFGF are mesodermal induction in early embryos and limb and lens regeneration.
Specificity
Human bFGF is effective on mouse, bovine, and human cells.ApplicationBasic fibroblast growth factor has been used as one of the components of EBM-B (endothelial Basal Medium-2 media, heat-inactivated fetal bovine serum, Glutamine-penicillin-streptomycin sulfate and basic fibroblast growth factor) media.
Quality
Endotoxin level: <0.1 EU/µg (LAL-test)
Note: 1 EU corresponds to 0.1 ng
Sequence
The primary structure of recombinant human bFGF (one polypeptide chain, 155 amino acids) is the biologically active precursor of natural human bFGF (one polypeptide chain, 146 amino acids), with the 9 additional amino acids located at the amino-terminus.
Chain Length 155 AA
Unit Definition
EC50 definition: The concentration of hbFGF that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with Balb/c 3T3 (NR6) cells.
Preparation Note
Working concentration: 0.5 to 10 ng/ml
For use in serum-free cell culture.
Working solution: Reconstitute the lyophilizate in sterile PBS (final concentration 100 µg/ml). Dilute further with PBS or medium containing 1 mg/ml BSA or 1 to 10% serum
Storage conditions (working solution): -15 to -25 °C
Reconstituted solution can be stored in aliquots at -15 to -25 °C.
Stable in culture at least for 3 days.
Note: Avoid repeated freezing and thawing.Other NotesRecombinant Basic Fibroblast Growth Factor, human (hbFGF), is produced in E. coli and purified by standard chromatographic techniques.
Specific activity/EC50:<1 ng/ml, at least the same specific activity (EC50) compared to the indicated standard is guaranteed.
For life science research only. Not for use in diagnostic procedures.
Contents
Lyophilizate, before lyophilization filtered through a 0.2 µm pore size membraneПараметрыbiological source human Quality Level 100, sterility non-sterile; 0.2 µm filtered assay >95% (SDS-PAGE) form lyophilized (salt-free) potency <1.0 ng/mL mol wt 18,000 kDa packaging pkg of 10 µg (11123149001), pkg of 25 µg (11120417001) Торговая марка Roche storage condition avoid repeated freeze/thaw cycles impurities <0.1 EU/µg shipped in dry ice storage temp. 20°C
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BCIP
Empirical Formula (Hill Notation): C8H6BrClNO4P · C7H9N CAS Number: 6578-06-9 Molecular Weight: 433.62 MDL number: MFCD00040636 PubChem Substance ID: 329774194
Кат. номер 11585002001 10760994001 General descriptionBCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.ApplicationFor the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Quality
Performance test: incubation with AP
Substrates
Histochemical substrate for alkaline phosphatase.
Analysis Note
Absorption: Absorption maximum in the visible light is not determined.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationBCIP is a registered trademark of Sigma-Aldrich Co. LLCПараметрыQuality Level 100, assay 99% (HPLC) form powder mol wt Mr 326.44 (BCIP), Mr 433.6 (BCIP toluidine salt) packaging pkg of 1 g (11585002001), pkg of 250 mg (10760994001) Торговая марка Roche solubility DMF: soluble shipped in wet ice storage temp. 2-8°C SMILES string Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23 InChI 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 InChI key QEIFSLUFHRCVQL-UHFFFAOYSA-N
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Bestatin 10874515001
Empirical Formula (Hill Notation): C16H24N2O4 · HCl CAS Number: 65391-42-6 Molecular Weight: 344.83 Beilstein/REAXYS Number: 4628066 MDL number: MFCD00058004 PubChem Substance ID: 329749214 ApplicationBestatin has been used as a protease inhibitor for the purification of his-tagged Tau protein.
Biochem/physiol Actions
A metalloprotease inhibitor selective for aminopeptidase. Bestatin is a competitive and specific inhibitor of leucine aminopeptidase, aminopeptidase B, and triamino peptidase. It inhibits aminopeptidase B at 60 nM (using arginine--naphthylamide as substrate) and leucine aminopeptidase at 20 nM (leucine--naphthylamide as substrate). It showed no inhibition of aminopeptidase A, trypsin, chymotrypsin, elastase, papain, pepsin, or themolysin. It offers promise as a novel analgesic because it protects endogenous opioid peptides against degradation.
Bestatin exhibits anti-angiogenic property and inhibits new blood vessel formation at the early stage of tumor growth and in the metastatic sites.
Preparation Note
Working concentration: 40 µg/ml (130 µM)
Working solution: Recommended solvents are 1 M HCl, methanol or 0.15 M NaCl.
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl.
For best results, use a stock solution of 2–5 mg/ml in methanol.
Storage conditions (working solution): -15 to -25 °C
Avoid repeated freezing!
Reconstitution
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl. Roche recommends preparing a stock solution of 2 to 5 mg/ml in methanol.
Solutions are stable for 6 months when stored in aliquots at -15 to -25 °C.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, assay 95% (HPLC) form crystalline powder packaging pkg of 10 mg Торговая марка Roche storage condition protect from light mp 195-205 °C solubility water: soluble 25 g/L, alcohol: soluble 5 g/L max 255-260 nm at 1 N in hydrochloric acid shipped in wet ice storage temp. 2-8°C SMILES string O=C(N[C@@H](CC(C)C)C(O)=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1.Cl[H] InChI 1S/C16H24N2O4.ClH/c1-10(2)8-13(16(21)22)18-15(20)14(19)12(17)9-11-6-4-3-5-7-11;/h3-7,10,12-14,19H,8-9,17H2,1-2H3,(H,18,20)(H,21,22);1H/t12-,13+,14+;/m1./s1 InChI key XGDFITZJGKUSDK-UDYGKFQRSA-N
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Biotin RNA Labeling Mix 11685597910
General descriptionConvenient nucleotide mixture for the labeling of RNA with Biotin-16-UTP.
Specificity
Heat inactivation: Stop the reaction by adding 2 µl 0.2 M EDTA (pH 8.0).ApplicationBiotin RNA Labeling Mix has been used for RNA labeling with Biotin-16-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases.
Biotin-labeled RNA is also used in a variety of hybridization techniques:- Southern blots
- Northern blots
- Dot blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridizations
- Microarray hybridizations
- RNA pull down assay
Features and Benefits
Contents
10x concentrated solution with: 10mM each ATP, CTP, GTP, 6.5mM UTP, 3.5mM Biotin-16-UTP, pH 7.5
Quality
Function tested in combination with the DIG RNA Labeling Kit.
Principle
Biotin-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Biotin-16-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under the conditions described below. The Biotin RNA Labeling Mix is specifically designed for the use with Roche SP6,T7, and T3 RNA Polymerases, which are supplied with an optimized transcription buffer.
Preparation Note
Assay Time: 135 minutes
Sample Materials- Linearized plasmid DNA: The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of run off transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5-overhangs should be used; 3 overhangs should be avoided. The linearized template DNA should be purified by phenolchloroform extraction and ethanol precipitation, to avoid RNase contamination. For run around transcription circular plasmid DNA is used.
- PCR product: PCR-fragments which contain RNA polymerase promoter sequences can also act as templates for transcription. Purification of the correct PCR fragment by gel electrophoresis prior to transcription is recommended.
- Labeling Efficiency :A standard labeling reaction with 1µg linear template DNA and an RNA polymerase produces approx. 10µg of full-length, biotin-labeled RNA. In a spot test, a combination of anti-biotin-AP and the chemiluminescence substrate CSPD can detect as little as 0.3pg of the biotin-labeled RNA.
Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, usage sufficient for 20 reactions (transcription) packaging pkg of 40 μL Торговая марка Roche shipped in dry ice storage temp. −20°C
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Biotin-16-ddUTP 11427598910
Empirical Formula (Hill Notation): C32H52N7O17P3S CAS Number: 188755-42-2 Molecular Weight: 931.78 General descriptionBiotin-16-ddUTP, tetralithium salt; 1mMApplication- Biotin-16-ddUTP is used as a substrate for: Terminal Transferase
- DNA polymerase I (Holoenzyme and Klenow fragment)
- T4 DNA polymerase
- Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor).
- The biotin-labeled oligonucleotide can be used as a hybridization probe for: DNA and RNA transfers
- Colony and plaque screening
- In situ hybridization
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3 end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine- triphosphate (biotin-ddUTP).
It has been used in the biotinylation of in vitro transcribed tmRNA during surface plasmon resonance (SPR) technique. It has been used in the standard nick translation reaction for tumor DNA labelling during comparative genomic hybridization.
Features and Benefits
Biotin is bound to dideoxyuridine triphosphate via an amide linkage.
Quality
Typical analysis: At least 85% Biotin-16-ddUTP (HPLC, area%).Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, mol wt 955.5 (biotin-16-ddUTP-Li4) packaging pkg of 25 µL (25 nmol; 1mM) Торговая марка Roche shipped in dry ice storage temp. 20°C
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Biotin-16-dUTP 11093070910
Empirical Formula (Hill Notation): C32H52N7O18P3S CAS Number: 136632-31-0 Molecular Weight: 947.78 General descriptionBiotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.ApplicationFor nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation.- Biotin-16-dUTP is used as asubstrate for: Terminal Transferase
- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., Transcriptor)
The nucleotide also serves as a substrate for Terminal Transferase in 3-end labeling.- Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
- Biotin Luminescence Detection Kit
Biochem/physiol Actions
Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.
Quality
Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Analysis Note
Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, packaging pkg of 50 μL (50 nmol; 1mM) Торговая марка Roche shipped in dry ice storage temp. −20°C
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Bln I (Avr II)
Кат. номер 11558170001 11558161001 General descriptionBln I recognizes the sequence CCTAGG and generates fragments with 5-cohesive termini.
Compatible ends
Bln I ends are compatible with ends generated by Nhe I, Spe I and Xba I.
Isoschizomers
Bln I is an isoschizomer of Avr II.
Note: The complete 13 site Avr II restriction map of the E.coli genome has been reported.
Methylation sensitivity
The enzyme is not known to be affected by methylation.
Specificity
Recognition sites: CCTAGG
CCTAGG
Restriction site: CCTAGG
CCTAGG
Heat inactivation: No inactivation of Bln I after incubation at 65 °C for 15 minutes.
Quality
Absence of nonspecific endonuclease activities
1 µg DNA is incubated for 16 hours in 50 µl SuRE/Cut Buffer H with an excess of Bln I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5 µg [3H] labeled calf thymus DNA are incubated with 3 µl Bln I for 4 hours at +37°C in a total volume of 100 µl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Typical ligation and recutting assay
Bln I fragments obtained by complete digestion of 1 µg x EcoR I DNA ligated for 16 hours at +4°C with 1 U T4 DNA Ligase in 10 µl buffer that contains 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Bln I and EcoR I (yielding the typical pattern of x EcoR I x Bln I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
DNA Profile
Number of cleavage sites on different DNAs- : 2
- X174: 0
- Ad2: 2
- M13mp7: 0
- M13mp18:0
- pBR322: 0
- pBR328: 0
- pUC18: 0
- SV40: 2
Unit Definition
One unit is the enzyme activity that completely cleaves 1 µg x EcoR I DNA fragments in one hour at +37 °C in a total volume of 25 µl (1x) SuRE/Cut Buffer H.
Analysis Note
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity- A: 25-50%
- B: 50-75%
- H: 100%
- L: 0-10%
- M: 25-50%
Activity in PCR buffer: Not tested
PFGE tested
Bln I has been tested in Pulsed-Field Gel Electrophoresis (on bacterial chromosomes). For cleavage of genomic DNA (E.coli C 600) embedded in agarose for PFGE analysis, we recommend using 10 U of enzyme/µg DNA and 4 hour incubation.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form solution packaging pkg of 1,000 U (11558170001 [10 U/µl]), pkg of 200 U (11558161001 [10 U/µl]) Торговая марка Roche parameter 37 °C optimum reaction temp. shipped in dry ice storage temp. 20°C
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Blocking Reagent 11096176001
ApplicationThe Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry.
Preparation Note
Working concentration: Dilution of Antibody 1:5,000
Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven.
Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form powder packaging pkg of 50 g Торговая марка Roche shipped in ambient storage temp. 20-25°C
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Blocking Reagent 11112589001
General descriptionBlocking reagent is used for blocking of nonspecific binding sites in ELISA procedures.ApplicationFor research use only. Not for use in diagnostic procedures.
Blocking reagent has been used as a blocking reagent in:- ELISA
- Immunofluorescence
- In situ hybridization
Preparation Note
Storage conditions (working solution): Autoclaved stock solution can be stored for several days to one week either unopened at 15 to 25 °C or at 2 to 8 °C after opening. Alternatively, the stock solution can be stored in aliquots at -15 to -15 °C for up to 6 months.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form powder packaging pkg of 27 g Торговая марка Roche storage temp. 2-8°C
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BM Blue POD Substrate, soluble 11484281001
Empirical Formula (Hill Notation): C16H20N2 CAS Number: 54827-17-7 Molecular Weight: 240.34 Beilstein/REAXYS Number: 2808541 MDL number: MFCD00007748 PubChem Substance ID: 329749453 ApplicationBM Blue POD Substrate, soluble, is ideal for ELISA applications. It has been used in fluorescein-tagged exendin-4 binding assay.
Specifications
Formula: C16H20N2
Color: blue; yellow after reaction is stopped
Evaluation: photometric (450 nm)
Physical form
Solution, ready-to-use
Analysis Note
Absorption: Identical to TMB absorption spectrumOther NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form solution (ready-to-use) mol wt Mr 240.35 packaging pkg of 100 mL Торговая марка Roche solubility water: soluble shipped in wet ice storage temp. 2-8°C SMILES string Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2 InChI 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 InChI key UAIUNKRWKOVEES-UHFFFAOYSA-N
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BM Chemiluminescence ELISA Substrate (POD) 11582950001
General descriptionThe sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.ApplicationThe BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.
Features and Benefits- Improved sensitivity
- Large dynamic range
- Rapid and constant signal
Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)
Principle
Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.
Preparation Note
Working solution: Preparation of working solution
The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use:- Add to 100 parts of solution A one part of solution B.
- Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, packaging pkg of 250 mL (for 2500 wells or 1000 tubes) Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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BM Chemiluminescence Western Blotting Kit (Mouse/Rabbit) 11520709001
General descriptionDetection of membrane-bound molecules is carried out by a variety of procedures. In protein analysis, the molecules of interest are usually detected with specific first antibodies, enzyme-labeled secondary reagents (antibody- or streptavidin-conjugates) and a suitable chromogenic substrate. These detection systems, however, may be time-consuming or lacking sensitivity. In addition, colors tend to fade with time and exposure to light.
To overcome these problems, attempts have been made to use chemiluminescence for fast and sensitive detection of enzyme conjugates on blots. The BM Chemiluminescence Western Blotting System is designed around peroxidase-labeled secondary antibodies and the substrate luminol. In the presence of hydrogen peroxide (H2O2), horseradish peroxidase (POD, HRP) catalyzes the oxidation of diacylhydrazides such as luminol. An activated intermediate reaction product is formed, which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by 4-iodophenol. This acts as a radical transmitter between the formed oxygen radical and luminol.ApplicationBM Chemiluminescence Western Blotting Kit (Mouse/Rabbit) is used to detect proteins with peroxidase-labeled secondary antibodies and the chemiluminescent substrate luminol on western blots. The chemiluminescence system is specifically suited for western and dot blot applications where high sensitivity is required.<<- High sensitivity: Detection with this kit is as sensitive as radioactive detection.
- Fast: Short exposure times ranging from a few seconds to one hour for obtaining highly sensitive results.
- Nonradioactive: Safe and produces no radioactive waste.
- Stable hard-copy results on film: No fading occurs during storage.
- Quantifiable results: Bands or dots on films can be quantified with a densitometer.
- Economically: Detection of antigen with low amounts of antibody or low affinity antibody.
Packaging
1 kit containing 4 components.
Principle
Each lot is function tested on a dot blot.
The BM Chemiluminescence Western blotting System is designed for peroxidase-labeled secondary antibodies and the substrate luminol. In the presence of hydrogen peroxide (H202), horseradish peroxidase (HRP) catalyzes the oxidation of diacylhydrazides, such as luminol. An activated intermediate reaction product is formed, which decays to the ground state by emitting light. Strong enhancement of the light emission is produced by 4-iodophenol. This acts as a radical transmitter between the formed oxygen radical and luminol.
Preparation Note
Working solution: Preparation of additional reagents and solutions
The table inside the PI describes the preparation of working solutions. For reproducible results equilibrate all solutions to 20 to 25 °C before use.
Storage conditions (working solution):- 1% Blocking solution: -15 to -25 °C
- 0.5% Blocking solution: -15 to -25 °C
- POD-labeled secondary antibody stock solution: 12 month at 2 to 8 °C
- POD-labeled secondary antibody working solution: prepare always fresh
- Detection solution: 1 week at 2 to 8 °C
Reconstitution
Reconstitute lyophilizate in 100 µl double-dist. water. This solution is stable for 12 months at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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BM Chemiluminescence Western Blotting Substrate (POD)
Кат. номер 11500708001 11500694001 ApplicationBM chemiluminescence western blotting substrate (POD) has been used along with horseradish peroxidase-coupled secondary antibodies, to visualize cells on the membranes.
Features and Benefits
Sensitivity: <10pg antigen in western blots. Shows at least 10-fold higher sensitivity than other nonradioactive or radioactive detection systems on PVDF membranes (using -galactosidase, anti--galactosidase, or anti-mouse IgG-POD, Fab fragments).
Packaging
1 kit containing 3 components
Quality
Each lot is function tested in a dot blot.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыpackaging pkg of 1 set (11500694001 [4,000 cm2 membrane]), pkg of 1 set (11500708001 [1,000 cm2 membrane]) Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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BM Condimed H1 11088947001
General descriptionBM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Cell Line Origin
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.ApplicationBM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
Features and Benefits
Contents
Solution in RPMI 1640, filtered through 0.2µm pore size membrane
Physical form
Solution in RPMI 1640, filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.
Analysis Note
Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, sterility 0.2 μm filtered form solution packaging pkg of 100 mL (10x) Торговая марка Roche application(s) cell culture | hybridoma: suitable solubility water: miscible shipped in dry ice storage temp. −20°C
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BM-Condimed 10663573001
General descriptionBM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.ApplicationBM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.
Physical form
Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate.
Preparation Note
Working concentration: 1 to 100µg/ml
1 to 100µg/ml
Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
Storage and Stability
Store at 2 to 8 °C. (Store in dark!)Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыsterility 0.2 μm filtered Quality Level 100, form solution packaging pkg of 100 mL Торговая марка Roche application(s) cell culture | mammalian: suitable solubility water: miscible shipped in wet ice
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BM-Cyclin 10799050001
General descriptionBM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.
Specificity
Sensitive Organisms: Mycoplasmas and bacteria- BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
- Mycoplasma arginini,
- Mycoplasma hyorhinis, and
- Mycoplasma orale
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.ApplicationBM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.
Packaging
Set of two different antibiotics
Physical form
Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution
Preparation Note
Working concentration: 10µg/ml pleuromutilin derivative (BM-cyclin 1), 5µg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1µg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form lyophilized packaging pkg of 37.5 mg (for 2 x 2.5 l medium) Торговая марка Roche Mode of action protein synthesis | interferes antibiotic activity spectrum mycoplasma shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H302 - H315 - H319 - H335 pcodes P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233 Target Organs Respiratory system RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F Not applicable Flash Point C Not applicable Узнать цену -
BM-Purple 11442074001
General descriptionBM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.
Appearance prior to use: light yellow color;
After color development: dark purpleApplication- Colorimetric AP substrate for: Immunohistocytochemistry
- Western blot
- Southern blot
- Northern blot
- Colony and plaque hybridization
- In situ hybridization
Components
Ready-to-use solution.
Preparation Note
Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.
Working solution: Solubility in water: insoluble.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, packaging pkg of 100 mL Торговая марка Roche color dark purple solubility H2O: insoluble shipped in wet ice storage temp. 2-8°C
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Bovine Serum Albumin 10711454001
General descriptionThe product, bovine serum albumin, is of special quality for molecular biology. Bovine serum albumin has homology with human serum albumin. Serum albumins are soluble proteins of the circulatory system and participate in various cellular processes.
Contents
Solution of 20mg/ml Bovine Serum Albumin in 50mM Tris-HCl, 0.1M NaCl, 0.25mM EDTA, 1mM 2-mercaptoethanol, 50% glycerol (v/v), pH approximately 7.6.ApplicationBovine Serum Albumin supports enzyme stability. It has been used for the:- preparation of circular DNA molecules
- amplification of bacterial 16S rRNA genes
- BOX-PCR fingerprinting
Quality
Contaminants: Albumin is checked for the absence of DNases, nicking activity, RNases, and proteases according to the current Quality Control procedures.
Physical form
Solution of 20 mg/ml Bovine Serum Albumin in 50 mM Tris-HCl, 0.1 M NaCl, 0.25 mM EDTA, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), pH approx. 7.6.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine form solution packaging pkg of 1 mL (20 mg/ml) Торговая марка Roche pH 4.8-7.5 shipped in dry ice storage temp. −20°C (−15°C to −25°C)
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Bovine Serum Albumin Fraction V
Кат. номер 10735078001 10735108001 10735094001 10735086001 General descriptionFor standard applications.ApplicationBovine Serum Albumin Fraction V is suitable for standard applications such as:- Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
- Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
- Reducing agent
- Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
- Media supplement for media that require addition of protein
- Carrier protein
- Standard protein in gel electrophoresis and protein determination
Quality
Purity: 95% protein, 5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)
Preparation Note
Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 biological source bovine assay 98.5% form lyophilized mol wt Mr =68 kDa packaging pkg of 1 kg (10735108001)","pkg of 100 g (10735086001)","pkg of 50 g (10735078001)","pkg of 500 g (10735094001) Торговая марка Roche application(s) ELISA: suitable","Southern blotting: suitable","western blot: suitable absorption 0.4 at 550 nm UniProt accession no. P02769, shipped in wet ice storage temp. 2-8°C Gene Information bovine ... ALB(280717)
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Bovine Serum Albumin Fraction V, fatty acid free 10775835001
General descriptionHighest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.ApplicationBovine Serum Albumin, Fraction V, fatty acid free is used:- in lipid research to study lipid metabolism in biological systems
- to identify transport mechanisms in living organisms
- to study ligand binding
- to investigate ionic binding
- in the preparation of solutions during isolation of cardiomyocytes
- as a blocking solution to incubate embryos for immunofluorescence
- in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers
Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Features and Benefits- Free from fatty acids according to current quality control procedures (suitable for lipid research)
- High quality
- Low content of heavy metal ions
- Long-term stability
Quality
Purity: 98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), 5% H2O,30 ppm heavy metalsOther NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100 biological source bovine assay 98.5% (electrophoresis) form lyophilized mol wt Mr 68 kDa packaging pkg of 50 g Торговая марка Roche application(s) immunofluorescence: suitable","ligand binding assay: suitable UniProt accession no. P02769, shipped in wet ice storage temp. 2-8°C Gene Information bovine ... ALB(280717)
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Bovine Serum Albumin Fraction V, heat shock
Кат. номер 3116964001 3116956001 Other NotesFor life science research only. Not for use in diagnostic procedures.
Loss on drying: ≤5.0%ПараметрыQuality Level 100, biological source bovine assay 98.5% form lyophilized mol wt Mr =68,000 packaging pkg of 1 kg (03116964001), pkg of 250 g (03116956001) Торговая марка Roche solubility 0.40 (A550) absorption 0.40 at 550 nm shipped in wet ice storage temp. 2-8°C
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Bovine Serum Albumin Fraction V, heat shock, fatty acid free 3117057001
General descriptionBSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.Application- Pharmacological assays; ion-binding, transport, or immunological studies
- Fatty acid and lipid research
- Stabilization of proteins and enzymes against proteolysis and denaturation
Biochem/physiol Actions
The fatty acids that are insoluble in circulating plasma are transported primarily by serum. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.
Quality
Purity: 98% albumin (electrophoresis), 98% protein
Contaminants: 1.2mg total fatty acids/g dry powder, 30ppm heavy metals, immunoglobulins are not detectable (by Ouchterlony)
Specifications
Moisture: 5%Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine assay 98% form lyophilized packaging pkg of 100 g Торговая марка Roche pH ~7.0 shipped in wet ice storage temp. 2-8°C
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Bovine Serum Albumin Fraction V, protease-free 3117332001
ApplicationBovine Serum Albumin Fraction V, protease-free is used:- for stabilization of purified enzymes
- for site-blocking reagent in ELISA techniques
- as a protein standard for determination of protein concentration
Quality
Contaminants: 0.001% heavy metals; proteases not detectable (casein digest)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, biological source bovine assay ≥=98.5% albumin basis (electrophoresis) form lyophilized packaging pkg of 50 g Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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Buffers in a Box, Premixed TBE Buffer, 10x 11666703001
General description10x solution
Quality
Absence of contaminants: free of DNase and RNase according to the current Quality Control procedures.
Physical form
Solution, clear, colorlessOther NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform solution Quality Level 100, packaging pkg of 4 L Торговая марка Roche color clear colorless pH ~8.4 shipped in ambient storage temp. 20-25°C
Safety Informationpictograms GHS08 signalword Danger hcodes H360FD pcodes P201 - P202 - P280 - P308 + P313 - P405 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 1 Flash Point F does not flash Flash Point C does not flash Узнать цену -
Calpain Inhibitor I 11086090001
Empirical Formula (Hill Notation): C20H37N3O4 CAS Number: 110044-82-1 Molecular Weight: 383.53 Beilstein/REAXYS Number: 7656053 MDL number: MFCD00065505 PubChem Substance ID: 329749147 General descriptionCalpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I and calpain II.
Specificity
Calpain Inhibitor I is a strong, competitive inhibitor of the Ca2+-dependent neutral cysteine proteases calpain I (requiring 2–75 µM Ca2+ for activation) and calpain II (requiring 200–800 µM) with a Ki-value between 0.12 µM and 0.23 µM. Calpain II is inhibited to a lesser extent than calpain I. Calpain Inhibitor I is also a strong inhibitor of papain and cathepsin B and L. It shows only weak inhibition of cathepsin H and -chymotrypsin and does not inhibit trypsin. ID50 for 0.02 U platelet calpain is 0.05 µM.ApplicationCalpain inhibitor I inhibits calpain, which activates myosin light chain kinase and protein kinase C by partial proteolysis. The inhibitor should be membrane permeable due to low molecular weight and lack of charged residues.
Calpain Inhibitor I is used for western blotting methods.
Quality
Purity: 98% (from C); chromatographically homogeneous
Formula variant
C20H37N3O4
Preparation Note
Working concentration: 0.1 - 10 µM
The suggested starting concentration is 17 µg/ml. This is the concentration at which half maximal inhibition of calpain I is observed.
Working solution: Recommended solvent is DMF, ethanol, or methanol up to 10 mg/ml.
Storage conditions (working solution): -15 to -25 °C
Solutions in DMF, methanol, ethanol are stable for 4 weeks at -15 to -25 °C.
Reconstitution
Soluble in DMF, ethanol, or methanol to 10 mg/ml. For a stock solution, Roche recommends dissolving 1 mg of the inhibitor in 100 μl DMF, methanol, or ethanol. Before use, dilute with water or phosphate buffer (0.1 M, pH 7.5) to desired concentration. Solutions in DMF, ethanol, or methanol are stable for 2 to 3 days at 2 to 8 °C, and approximately 4 weeks at -15 to -25 °C. For best results, prepare solutions fresh before use.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, assay 98% (chromatographically homogeneous) form powder mol wt Mr = 383.5 packaging pkg of 25 mg Торговая марка Roche mp 182 °C solubility DMF: 10 mg/mL, ethanol: 10 mg/mL, methanol: 10 mg/mL shipped in wet ice storage temp. 2-8°C SMILES string [H]C(=O)[C@H](CCCC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O InChI 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1 InChI key FMYKJLXRRQTBOR-BZSNNMDCSA-N
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Carboxypeptidase B, recombinant 8039852001
Enzyme Commission (EC) Number: 3.4.17.2 ( BRENDA | IUBMB ) ApplicationUse Carboxypeptidase B for the sequence analysis of proteins by successive cleavage of basic amino acids from the C-terminus of proteins.
Peptidyl-L-lysine (-L-arginine) hydrolase
Biochem/physiol Actions
Carboxypeptidase B is a Zn2+-metalloprotease, catalyzing the hydrolysis of the basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides.
Quality
Contaminants: Contaminants expressed as percentage of carboxypeptidase B activity.
Carboxypeptidase A 2%
Trypsin (with Chymotrypsin as substrate) 0.005 U/mgOther NotesFor life science research use only. Not for use in diagnostic precedures.Параметрыform solution Quality Level 100, specific activity ~150 units/mg protein (at +25°C with hippuryl-L-arginine as the substrate) shelf life A decrease in activity of approx. 10% may occur within 6 months. mol wt 34.6 kDa packaging pkg of 1 mL (2.8 mg) Торговая марка Roche storage condition avoid repeated freeze/thaw cycles optimum pH 7.0-9.0 shipped in dry ice storage temp. 20°C
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Carnitine Acetyltransferase 10103241001
Enzyme Commission (EC) Number: 2.3.1.7 ( BRENDA | IUBMB ) General descriptionCarnitine acetyltransferase is a mitochondrial enzyme essential for fuel utilization. It is present in the mitochondrial matrix and peroxisome. It is involved in the fatty acid metabolism. It facilitates conversion of acetyl-CoA and carnitine to acetylcarnitine and CoA respectively. This conversion facilitates peroxisomal β-oxidation by importing acetyl group for mitochondrial oxidation.
Acetyl-CoA:carnitine O-acetyltransferase
Quality
Contaminants: <0.01% acetyl-CoA-deacylase ion in storage buffer.
Physical form
Suspension in 2.9 M ammonium sulfate solution, 50 mM K-phosphate, 1 mM EDTA, pH approximately 7.5Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, form suspension specific activity ~80 units/mg protein (At 25 °C with CoA and acetyl-D,L-carnitine as the substrates.) packaging pkg of 1 mL (5 mg) Торговая марка Roche optimum pH 7.3-8.0 shipped in wet ice storage temp. 2-8°C
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CAT ELISA 11363727001
General descriptionPromoter activity in transfected eukaryotic cells is generally studied by linking the promoter sequence to a gene that encodes an easily detectable “reporter” protein, such as chloramphenicol acetyltransferase (CAT), -galactosidase (-Gal), or luciferase (Luc).
The bacterial enzyme chloramphenicol acetyltransferase (CAT, type I), encoded by Tn9 and having no eukaryotic equivalent, has become one of the standard markers used in transfection experiments. Traditionally, CAT activity is measured using a radioisotopic CAT assay.
The CAT ELISA is specific for CAT (type I) (Acetyl-CoA:chloramphenicol acetyltransferase, EC 2.3.1.28) from E. coli (Tn9-encoded).ApplicationFor research use only. Not for use in diagnostic procedures.
The CAT ELISA is used to quantitatively measure CAT expression in eukaryotic cells that are transfected with a plasmid bearing a CAT-encoding reporter gene.
Features and Benefits
The CAT ELISA is specific for CAT (type I) (Acetyl-CoA:chloramphenicol acetyltransferase, EC 2.3.1.28) from E. coli (Tn9-encoded).- Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used
- Faster: Approximately 4hours from start to finish
- Safer: No radioisotopes are used
- More accurate: Measures the actual amount of CAT protein synthesized, not just CAT enzyme activity
- Sensitive: Equivalent to the sensitivity of the radioactive CAT assay
- Easy to perform: Follows a standard ELISA protocol in microplates; allows Triton lysis of the transfected cells (CAT activity in the radioisotopic assay is rapidly inhibited by Triton)
- More flexible: Allows the analysis of promoters in plant protoplasts (alternative method to the GUS assay) and animal cells
Packaging
1 kit containing 10 components.
Principle
The CAT ELISA is based on the sandwich ELISA principle. Following lysis of the transfected cells, the cell extracts (that contain CAT enzyme) are added to the wells of the microplate, which is precoated with a polyclonal antibody to CAT (anti-CAT). All CAT contained in the cell extracts binds to the anti-CAT antibody that is bound to the plate surface. Next, a digoxigenin-labeled antibody to CAT (anti-CAT-DIG) is added and binds to CAT. In the next step, an antibody to digoxigenin, conjugated to peroxidase (anti-DIG-POD), is added and binds to digoxigenin. In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate, yielding a colored reaction product. The absorbance of the sample is determined using an ELISA reader and is directly correlated to the level of CAT present in the cell extracts. The sensitivity of the assay can be enhanced using ABTS with the substrate enhancer.
Preparation Note
Working solution: CAT enzyme working dilution
Add 40 µl CAT enzyme stock solution (solution 1) to 3.96 ml Sample buffer (solution 7) to obtain a CAT enzyme working dilution (final concentration approx. 1 ng/ml), sufficient to produce a calibration curve in duplicate.
Handling instructions- The CAT enzyme standard dilutions should be prepared freshly before use and should not be stored.
- Prepare the standard dilution series in reaction tubes in 1:2 dilution steps as described in the table below.
- To obtain a calibration curve, we recommend using the five concentrations listed.
- 200 µl of each dilution is needed per well.
- To ensure that the measurements and the calibration curve are accurate, we recommend preparing two samples of each concentration for measurement.
- To avoid carryover of the higher conc. solution to the lower conc. samples, use a fresh pipette tip for each dilution step.
- Each dilution must be measured in duplicate.
Storage conditions (working solution): Sample Buffer 2 to 8 °C
Stability of ready-to-use sample buffer: 2 months at 2 to 8 °C, otherwise aliquoted at - 15 to -25 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, usage sufficient for 192 tests Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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Cell Death Detection ELISA 11544675001
General descriptionThe Cell Death Detection ELISA serves as photometric enzyme immunoassay for the qualitative and quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death. This assay is based on the quantitative sandwich-enzyme immunoassay-principle using mouse monoclonal antibodies directed against DNA and histones, respectively. The antibodies used are not species specific.
Specificity
Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, and Xenopus). Anti-DNA-POD antibody binds to ss- and dsDNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems.ApplicationCell Death Detection ELISA has been used to measure Caspase-3 activity, intracellular reactive oxygen species (ROS) and cell apoptosis.
For research use only. Not for use in diagnostic procedures.
Specific determination of mono- and oligonucleosomes in the cytoplasmic fraction of cell lysates.
Packaging
1 kit containing 9 components.
Preparation Note
Working solution: Coating solution
Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer.
Washing solution
Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly.
Sample solution
Preparation of the sample solution depends on the cell system used and the extent of cell death.
Example: Dilute 25 µl of sample in 225 µl Incubation buffer.
Conjugate solution
Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer.
Substrate solution
Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer.
Allow to come to 15 to 25 °C before use.
Note: The ABTS solution is light sensitive over extended periods of time.
Storage conditions (working solution): Anti-histone
2 to 8 °C for 2 months
Anti-DNA-POD
2 to 8 °C for 2 months
Coating solution
Prepare immediately before use!
Washing solution
2 to 8 °C for 2 months
Sample solution
2 to 8 °C for 2 months
Conjugate solution
Prepare immediately before use!
Substrate solution
2 to 8 °C for 1 month, protected from light.
Reconstitution
Anti-histone
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly.
Anti-DNA-POD
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыusage sufficient for 96 tests Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS07 signalword Warning hcodes H317 - H319 pcodes P261 - P280 - P333 + P313 - P337 + P313 - P362 + P364 - P501 RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F does not flash Flash Point C does not flash Узнать цену -
Cell Proliferation ELISA, BrdU (chemiluminescent) 11669915001
General descriptionChemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Specificity
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.ApplicationCell Proliferation ELISA, BrdU (chemiluminescent) has been used:- for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
- to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
- to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines
The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:- Detection and quantification of cell proliferation induced by growth factors and cytokines
- Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
- Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
- Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
Packaging
1 kit containing 7 components.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыusage sufficient for ≤1,000 tests Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H317 - H319 pcodes P210 - P233 - P261 - P280 - P303 + P361 + P353 - P370 + P378 RIDADR UN1170 - class 3 - PG 2 - Ethanol, solution WGK Germany WGK 2 Узнать цену -
Cell Proliferation ELISA, BrdU (colorimetric) 11647229001
General descriptionColorimetric immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis: a nonradioactive alternative to the [3H]-thymidine incorporation assay.
Specificity
The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.ApplicationFor research use only. Not for use in diagnostic procedures.
The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:- Detection and quantification of cell proliferation induced by growth factors and cytokines
- Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
- Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
- Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth
Packaging
1 kit containing 6 components.
Preparation Note
Working concentration: The kit antibody (Anti-BrdU-peroxidase) has, after reconstitution, a concentration of 7.5 U/ml, after dilution 0.075 U/ml. Instead of the kit antibody, you can also use the Anti-BrdU-Antibody, Fab fragments.This antibody is double-concentrated, so you have to dilute it after reconstitution in 1 ml with 1:200.
Working solution: BrdU labeling solution
Dilute BrdU labeling reagent 1:100 with sterile culture medium (resulting concentration: 100 µM BrdU).
For one 96-well MP, 1 ml BrdU labeling solution is required if the cells were cultured in 100 µl /well (10 µl/well) and 2 ml BrdU labeling solution is required if the cells were cultured in 200 µl/well (20 µl/well).
Anti-BrdU-peroxidase stock solution
Dissolve Anti-BrdU-peroxidase in 1.1 ml double-dist. water for 10 minutes and mix thoroughly.
Anti-BrdU-peroxidase working solution
Dilute Anti-BrdU-peroxidase stock solution 1:100 with antibody dilution solution. For one 96-well MP dilute 100 µl Anti-BrdU-peroxidase stock solution in 10 ml antibody dilution solution
Washing solution
Dilute Washing buffer concentrate 1:10 with double-dist. water.
For one 96-well MP dilute 10 ml Washing buffer concentrate with 90 ml double-dist. water.
Storage conditions (working solution): BrdU labeling solution
The undiluted BrdU labeling reagent (1000x): At 2 to 8 °C for several months protected from light.
The diluted BrdU labeling reagent: At 2 to 8 °C stable for several weeks. Store protected from light. For long-term storage it is recommended to store the BrdU labeling solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase stock solution
At 2 to 8 °C for several months. For long-term storage it is recommended to store the solution in aliquots at -15 to -25 °C.
Anti-BrdU-peroxidase working solution
Prepare shortly before use. Do not store.
Washing solution
At 2 to 8 °C for several weeks.
Reconstitution
The working solution for the antibody should be phosphate buffered saline containing 1% BSA, pH 7.4Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыusage sufficient for ≤1,000 tests Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
Safety Informationpictograms GHS02,GHS07 signalword Danger hcodes H225 - H317 - H319 pcodes P210 - P233 - P261 - P280 - P303 + P361 + P353 - P370 + P378 RIDADR UN1170 - class 3 - PG 2 - Ethanol, solution WGK Germany WGK 2 Узнать цену -
Cell Proliferation Kit I (MTT) 11465007001
General descriptionThe Cell Proliferation Kit I (MTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
MTT was the first tetrazolium salt described. It is cleaved to formazan by enzymes of the endoplasmic reticulum. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Colorimetric assay (MTT based) for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity.ApplicationThe Cell Proliferation Kit I (MTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:- Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth-inhibitory antibodies and physiological mediators
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth
Features and Benefits- Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
- Accurate: The absorbance obtained strongly correlates to the cell number.
- Sensitive: Detect low cell numbers.
- Fast: Process a large number of samples using a multi-well ELISA reader.
The assay is based on the cleavage of the tetrazolium salt MTT in the presence of an electron-coupling reagent. The water-insoluble formazan salt produced must be solubilized in an additional step. Cells grown in a 96-well tissue culture plate are incubated with the MTT solution for approximately 4 hours. After this incubation period, a water-insoluble formazan dye is formed. After solubilization, the formazan dye is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells (see below).
Packaging
1 kit containing 2 components.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
After thawing, the MTT labeling reagents may be stored protected from light at 2 to 8 °C for up to 4 weeks, in which case reagent filtration through a 0.2 µm pore size membrane is recommended.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform liquid Quality Level 100, usage sufficient for 2,500 tests packaging pkg of 1 kit Торговая марка Roche storage condition protect from light application(s) cell analysis: suitable, detection: suitable, tissue culture: suitable max 550-600 nm detection method colorimetric shipped in dry ice storage temp. 20°C
Safety Informationpictograms GHS05 signalword Danger hcodes H318 pcodes P280 - P305 + P351 + P338 + P310 RIDADR NONH for all modes of transport WGK Germany WGK 2 Flash Point F does not flash Flash Point C does not flash Узнать цену -
Cell Proliferation Kit II (XTT) 11465015001
General descriptionThe Cell Proliferation Kit II (XTT) is a colorimetric assay for the nonradioactive quantification of cellular proliferation, viability, and cytotoxicity. Sample material is either adherent or suspension cells cultured in 96-well microplates.
Colorimetric assays analyze the number of viable cells by the cleavage of tetrazolium salts added to the culture medium. This technique requires neither washing nor harvesting of cells, and the complete assay, from microculture to data analysis by an ELISA reader, is performed in the same microplate.
More recently, the tetrazolium salt XTT was described. In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required. The tetrazolium salt XTT is cleaved to formazan by a complex cellular mechanism. This bioreduction occurs in viable cells only, and is related to NAD(P)H production through glycolysis. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.ApplicationThe Cell Proliferation Kit II (XTT) is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for:- Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds.
- Assessment of growth-inhibitory antibodies and physiological mediators that inhibit cell growth.
- Testing of biocompatibility of various scaffolds, employed in bone tissue engineering, for bone cell growth.
- cell viability assay.
Features and Benefits- Safe and easy: Eliminate radioactive isotopes, washing steps, and additional reagents.
- Accurate: The absorbance obtained strongly correlates to the cell number.
- Sensitive: Detect low cell numbers.
- Fast: Process a large number of samples using a multi-well ELISA reader.
Packaging
1 kit containing 2 components.
Principle
The assay is based on the cleavage of the tetrazolium salt XTT in the presence of an electron-coupling reagent, producing a soluble formazan salt. This conversion only occurs in viable cells. Cells grown in a 96-well tissue culture plate are incubated with the XTT labeling mixture for 2 - 20 hours. After this incubation period, the formazan dye formed is quantitated using a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
Preparation Note
Working solution: Preparation of solutions
Thaw XTT labeling reagent and electron-coupling reagent, respectively in a water bath at 37 °C. Mix each vial thoroughly to obtain a clear solution.
XTT labeling mixture
To perform a cell proliferation assay (XTT) with one microplate (96 wells) mix 5 ml XTT labeling reagent with 0.1 ml electron coupling reagent.
Note: To obtain reliable results thaw and mix XTT labeling reagent and electron coupling reagent immediately before use.
Working instruction
Cells are grown in microplates (tissue culture grade, 96 wells, flat bottom) in a final volume of 100 µl culture medium per well, according to the media needs of the cells in a humidified atmosphere (e.g., 37 °C, 6.5% CO2).
The incubation period of the cell cultures depends on the particular experimental approach and on the cell line, used for the assay. For most experimental setups, the incubation of cells for 24 to 96 hours is appropriate.
After the incubation period, add to each well 50 µl of the XTT labeling mixture, prepared as described above (final XTT concentration 0.3 mg/ml).
Incubate the microplate for 4 to 24 hours in a humidified atmosphere (e.g., 37 °C, 6.5% CO2).
Note: The incubation time varies with the individual experimental setup (e.g., cell type and cell concentration, used). Therefore, we recommend to measure the absorption as described at different time points after addition of XTT labeling mixture (e.g., 4, 6, 8, 12, and 18 hours) using one and the same microplate to determine the optimal incubation period for the particular experimental setup.
Storage conditions (working solution): Thaw reagents immediately before use. It is recommended to prepare appropriate aliquots [5 ml XTT labeling reagent and 0.1 ml electron coupling reagent are required for the performance of the assay with one microplate (96 wells)]
Note: Avoid repeated thawing and freezing.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform liquid Quality Level 100, usage sufficient for 2,500 tests packaging pkg of 1 kit Торговая марка Roche storage condition protect from light application(s) cell analysis: suitable, detection: suitable, tissue culture: suitable max 450-500 nm detection method colorimetric shipped in dry ice storage temp. 20°C
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Cell Viability Imaging Kit 5x96 6432379001
General descriptionThe Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.
The kit is designed for 5 x 96 reactions for use in medium to high throughput analysis of cell behavior.- Determine total cell number using fluorescence staining of cell nuclei.
- Fluorimetrically determine the percentage of dead cells based on membrane permeability.
- Verify the labeling of viable cells quantified by fluorescence staining using a vital dye
ApplicationCell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.
Features and Benefits
Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:- No risk of losing cells in the one step assay: There are no fixation or washing steps.
- Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
- Obtain highly reproducible results using automated imaging and data evaluation with Roches Cellavista System.
Packaging
1 kit containing 3 components.
Preparation Note
Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.
Storage and Stability
Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)Other NotesFor life science research only. Not for use in diagnostic procedures.ПараметрыQuality Level 100, usage sufficient for 5 96-well plate(s) packaging pkg of 1 kit Торговая марка Roche storage condition protect from light fluorescence ex 361 nm; em 486 nm (nuclei dye), ex 490 nm; em 515 nm (viable dye), ex 535 nm; em 617 nm (dead dye) detection method fluorometric shipped in dry ice storage temp. 20°C (15°C to 25°C)
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Cellular DNA Fragmentation ELISA 11585045001
General descriptionThe Cellular DNA Fragmentation ELISA is a photometric enzyme-linked immunosorbent assay (ELISA) for the detection of 5-bromo-2-deoxy-uridine (BrdU)-labeled DNA fragments in culture supernatants and cell lysates. BrdU is used as a metabolic labeling agent by the nuclear DNA of target cells. This BrdU-labeled DNA can be detected easily and quantified using a monoclonal antibody against BrdU in an ELISA. This kit is a non-radioactive alternative to the [3H]-thymidine release assay, the [51Cr]-release assay, and the [3H]-thymidine DNA fragmentation assay.ApplicationFor research use only. Not for use in diagnostic procedures.
The Cellular DNA Fragmentation ELISA is for the quantification of BrdU-labeled DNA fragments either released from cells during necrosis or cell-mediated cytotoxicity, or within the cytoplasm of apoptotic cells.
Features and Benefits- Nonradioactive assay system
- Sensitive (1 x 103 cells/well)
- Low assay background
- No additional dilution steps required
- No washing of the cells required
- Results obtained correlate to those obtained by standard methods
- Denaturation/fixation of DNA in the microplate by microwave irradiation
- Fast (4.5 - 5.5 hours)
Packaging
1 kit containing 8 components.
Preparation Note
Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.
Storage conditions (working solution): Storage of coated plates: 1 week at 2 to 8 °C.
The anti-BrdU-POD stock solution is stable at 2 to 8 °C for several months; for long- term storage it is recommended to store the solution in aliquots at -15 to -25 °C;
The anti-BrdU-working solution should not be stored.
For the anti-DNA-clone MCA-33 we do no have stability data. However it should be no problem to aliquot and store this non-conjugated antibody at -15 to -25 °C for longer storage.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыusage sufficient for ≤500 tests Quality Level 100, Торговая марка Roche shipped in wet ice storage temp. 2-8°C
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CHAPS
Empirical Formula (Hill Notation): C32H58N2O7S · xH2O CAS Number: 75621-03-3 Molecular Weight: 614.88 (anhydrous basis)
Кат. номер 10810126001 10810118001 General description3-[(3-Cholamidopropyl)dimethyl-ammonio]-1-propane sulfonateApplicationSolubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.
Physical form
Crystals
Preparation Note
Working concentration: PAGE: 6.5 to 13 mM
Storage conditions (working solution): Do not store in solution, because precipitation may occur.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыassay 98% (from N) mol wt 614.88 g/mol (anhydrous basis) packaging pkg of 10 g (10810118001), pkg of 50 g (10810126001) Торговая марка Roche application(s) dialysis: suitable CMC 4 mM mp 157 °C (dec.) (lit.) solubility >50% (Aqueous solutions) absorption <0.06% at 278 nm in water at 1 % (w/v) (Ease of removal*: ++
Note:
* Arbitrary scale ranges from ++ to )shipped in wet ice storage temp. 2-8°C SMILES string O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C InChI 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1 InChI key SJCUTFKCLFLIFE-JWTJKVBLSA-N
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Chromozym PK 10378445001
General descriptionChromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.ApplicationUse Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
Quality
Contaminant: <0.5% free 4-nitraniline
Sequence
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
Principle
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
Preparation Note
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationChromozym is a registered trademark of Pentapharm AG, BaselПараметрыdescription Molecular Formula: C33H39N8O6COOCH3 Quality Level 100, assay 90% (enzymatic) form powder mol wt Mr 702.9 packaging pkg of 20 mg Торговая марка Roche shipped in ambient storage temp. room temp
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Chromozym PL 10378461001
General descriptionChromozym® PL is a chromogenic substrate. It is a synthetic tripeptide substrate and is more plasmin-specific.ApplicationChromozym® PL has been used:- to determine the plasmin enzymatic activity using human umbilical vein endothelial cells (HUVEC) cell extracts
- as a chromogenic substrate for proteolytic activity in murine lung homogenates
- as a substrate of plasmin in plasminogen activation assays
Quality
Purity: 90% Tosyl-Gly-Pro-Lys-4-nitranilide acetate (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Formula: C26H35N6O7SCOOCH3
Sequence
Tosyl-glycyl-polyl-lysine-4-nitranilide-acetate
Physical form
Powder
Preparation Note
Working concentration: Approximately 0.3 to 0.6 mM
Storage conditions (working solution): 2 to 8 °C
A substrate solution (3 mM) is stable for at least two weeks if stored at 2 to 8 °C. Avoid contamination with germs.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationChromozym is a registered trademark of Pentapharm AG, BaselПараметрыassay 90% Quality Level 100, form powder packaging pkg of 20 mg Торговая марка Roche shipped in ambient storage temp. room temp
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Chromozym t-PA 11093037001
General descriptionN-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide acetateApplicationUse Chromozym® t-PA (tissue plasminogen activator) (C24H32N8O7S x CH3COOH) as a substrate for the determination of t-PA, both in purified preparations and in cell culture supernatants. Moreover, it can be applied to evaluate the content of one-chain and two-chain t-PA.
Quality
Purity: >90% (enzymatic)
Contaminant: <0.5% free 4-nitraniline
Sequence
N-Methylsulfonyl-D-Phe-Gly-Arg-4-nitranilide-acetate
Preparation Note
Working concentration: Approximately 0.25 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym t-PA (4mM) is stable for at least two weeks if stored at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationChromozym is a registered trademark of Pentapharm AG, BaselПараметрыQuality Level 100, assay 90% (enzymatic) form powder mol wt 636.7 packaging pkg of 20 mg Торговая марка Roche concentration 0.25 mM shipped in wet ice storage temp. 20-25°C
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Chromozym TH
Кат. номер 11585398001 10206849001 General descriptionChromozym® TH is a chromophoric substrate mostly used to detect thrombin activity†
Tosyl-Gly-Pro-Arg-4-nitranilide acetateApplicationUse Chromozym® TH as a substrate for the determination of serine proteases, specifically thrombin in aqueous solutions.
Quality
Contaminant: 1% free 4-nitraniline
Sequence
Tosyl-glycyl-polyl-arginine-4-nitranilideacetate
Preparation Note
Working concentration: Approximately 0.2 mM
Storage conditions (working solution): 2 to 8 °C
A substrate solution (approx. 2 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.Other NotesFor life science research only. Not for use in diagnostic procedures.Legal InformationChromozym is a registered trademark of Pentapharm AG, BaselПараметрыassay 90% (Tosyl-Gly-Pro-Arg-4-nitranilide acetate (enzymatic)), 90% (enzymatic) Quality Level 100, form powder mol wt Mr 662.62 packaging pkg of 100 mg (11585398001), pkg of 20 mg (10206849001) Торговая марка Roche shipped in ambient storage temp. 20-25°C
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Chymostatin 11004638001
CAS Number: 9076-44-2 MDL number: MFCD00071059 PubChem Substance ID: 329749270 General descriptionChymostatin is a mixture of three components, A, B, and C. The component A being N-[((S)-1-carboxy-2-phenylethyl)-carbamoyl]--[2-iminohexahydro-4(S)-pyrimidyl]-L-glycyl-L-leucyl-phenylalaninal. The other two components B and C differ in that the L-leucyl residue is substituted by L-valine and L-isoleucine, respectively.ApplicationChymostatin is a specific inhibitor of α-, β-, γ-, and δ-chymotrypsin.
Biochem/physiol Actions
Chymostatin is a strong inhibitor of many proteases, including chymotrypsin, papain, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases such as cathepsins A,B,C, B, H, and L. It weakly inhibits human leucocyte elastase. It is effective at a final concentration of 100 to 200 μg/ml (10 to 100 μM). Chymostatin is often included in protease inhibitor cocktails used with plant extracts.
Quality
Performance tested.
Formula variant
C31H41N7O6
Preparation Note
Working concentration: 6 to 60 µg/ml (10 to 100 µM)
1 U chymotrypsin is inhibited to 49% of the original activity by 1.8 µg of chymostatin.
Thin-layer chromatography: butanol/methanol/H2O = 4 / 1 / 2
Working solution: Soluble in glacial acetic acid or DMSO to 20 mg/ml. Sparingly soluble in water, methanol, or ethanol. Insoluble in ethyl acetate, petroleum and ethyl ethers, hexane, or chloroform (CHCl3).
It is recommended to dissolve the inhibitor in 1% acetic acid in higher concentration and to adjust the concentration wanted with phosphate buffer, 0.05 M, pH 7.0, which is common for chymotrypsin assay.
CAUTION: DMSO (Dimethyl sulfoxide) will permeate the skin, carrying solubilized protease inhibitors. Always wear appropriate protection for eyes, skin, etc.
Storage conditions (working solution): -15 to -25 °C
Dilute solutions should be stored frozen in aliquots at -15 to -25 °C and are stable for approximately one month. Avoid repeated freezing. Growth of microorganisms should be avoided as proteases from microbial origin may hydrolyze the peptides.
Solubility testing in glacial acetic acid at 10 mg/ml yields a clear solution, which is usually colorless, but can be yellow in appearance. It is reportedly also soluble in DMSO; only slightly soluble in water and short-chain alcohols; insoluble in ethyl acetate, butyl acetate, ether, hexane, and petroleum ether. Stock solutions (10 mM) can be prepared in DMSO and are stable for months at -20 °C. Stock solutions can also be made in 0.1 M HCl. Dilute solutions (10-100 μM) are only stable for several hours, due to oxidation of the terminal aldehyde.Other NotesFor life science research only. Not for use in diagnostic procedures.Параметрыform powder Quality Level 100, mol wt Mr = 607.71 packaging pkg of 10 mg Торговая марка Roche mp 205 °C solubility acetic acid: soluble 20 mg/mL shipped in wet ice storage temp. 2-8°C SMILES string OC(C(NC(NC(C1NC(NCC1)=N)C([F,Cl,Br,I]C)=O)=O)CC2=CC=CC=C2)=O InChI key MRXDGVXSWIXTQL-HYHFHBMOSA-N
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