Roche

KAPA SYBR® FAST qPCR kit can be used for:

Gene expression
Gene knockdown validation
Microarray validation
ChIP analysis
Low copy detection
Absolute quantification of NGS libraries
Real-time PCR

Features and Benefits
Quantitate changes in gene expression more accurately

High reaction efficiency between 95 – 105% improves accuracy and reproducibility.
Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently

Improved processivity results in earlier Cq scores.
Higher fluorescence detection across varying AT- and GC-rich targets.
Novel enzyme is resistant to SYBR® inhibition.

Complete real-time PCR runs in just 40 minutes

55% shorter run times with fast cycling protocol.
Maintain high performance when switching from standard to fast protocols.

Quick Notes:

This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
Do not exceed 25 µL reaction volumes.

Quality
All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.
Preparation Note
Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) ABI PRISM® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

For Research Use Only. Not for use in diagnostic procedures.

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Quality Level	100,
shelf life	12 mo.
packaging	kit of 1 mL (100 x 20 µL rxn; KK4603), kit of 10 mL (1000 x 20 µL rxn; KK4605), kit of 5 mL (500 x 20 µL rxn; KK4604), kit of 50 mL (5000 x 20 µL rxn; KK4617)
Торговая марка	Roche
concentration	2 x
application(s)	qPCR: suitable
compatibility	for use with Applied Biosystems^® 5700, for use with Applied Biosystems^® 7000, for use with Applied Biosystems^® 7300, for use with Applied Biosystems^® 7700, for use with Applied Biosystems^® 7900HT, for use with StepOne^™, for use with StepOnePlus^™
shipped in	dry ice
storage temp.	20°C

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.
KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.

pictograms	GHS08
signalword	Warning
hcodes	H371
pcodes	P260 - P264 - P270 - P308 + P311 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA SYBR® FAST One-Step

Quality
All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.
Preparation Note
Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C. The KAPA RT Mix is temperaturesensitive, and should be stored at -20°C and kept on ice during use.
The SYBR Green I dye contained in the KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

KAPA SYBR® FAST One-Step

Gene expression analysis
Low-copy gene detection
Microarray validationmiRNA research
Gene knockdown validation
Real-time quantitative reverse transcription-PCR (qRT-PCR)
RNAi and miRNA research

Features and Benefits
Increase signal and reaction efficiency

Consistently higher amplification efficiencies for accurate qPCR.
Higher fluorescence, earlier Cq values and improved reaction efficiencies.

Amplify across a broad range of target lengths

Robust performance independent of amplicon size.
Improve sensitivity and reproducibility
Accurate interrogation across a wide range of RNA template concentrations.

Quick Notes:

This kit contains wild-type M-MuLV and an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
Use only gene-specific primers for one-step qRT-PCR.
Optimal cDNA synthesis is achieved at 42°C for 5 min.
3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
Do not exceed 25 µL reaction volumes.

For Research Use Only. Not for use in diagnostic procedures.

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Quality Level	100,
shelf life	6 mo.
packaging	kit of 1 mL (100 x 20 µL rxn; KK4650), kit of 10 mL (1000 x 20 µL rxn; KK4652), kit of 5 mL (500 x 20 µL rxn; KK4651)
Торговая марка	Roche
concentration	2 x
application(s)	RT-PCR: suitable
compatibility	for use with Agilent Mx3000P, for use with Agilent Mx3005P, for use with Agilent Mx4000, for use with Applied Biosystems^® 5700, for use with Applied Biosystems^® 7000, for use with Applied Biosystems^® 7300, for use with Applied Biosystems^® 7500, for use with Applied Biosystems^® 7700, for use with Applied Biosystems^® 7900HT, for use with Applied Biosystems^® ViiA 7, for use with QuantStudio^™, for use with StepOne^™, for use with StepOnePlus^™
detection method	SYBR^® Green
shipped in	dry ice
storage temp.	20°C

KAPA Taq EXtra HotStart® ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 µL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl2 (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.

pictograms	GHS08
signalword	Warning
hcodes	H371
pcodes	P260 - P264 - P270 - P308 + P311 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA Taq EXtra HotStart ReadyMix

KAPA Taq EXtra HotStart® ReadyMix™ has been used for:

PCR of amplicons to be analyzed by agarose gel electrophoresis
PCR of short and medium length targets (<5 kb)
Long-range PCR (5 – 15 kb)
PCR with limiting amounts of template DNA

Quality
Each batch of KAPA Taq EXtra HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

KAPA Taq EXtra HotStart® is a blend of KAPA Taq HotStart and a proofreading DNA polymerase
Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA
Fidelity is 2–4 times better than Taq
For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
Amplify mid- to long-range targets with high yield and sensitivity.
2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

feature	dNTPs included, hotstart
Quality Level	100,
packaging	pkg of 1.25 mL (KK3604), pkg of 6.25 mL (KK3605)
Торговая марка	Roche
application(s)	PCR: suitable
input	purified DNA
storage temp.	−20°C

KAPA Taq PCR Kit has been used in:

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KK1512
KAPA Taq HotStart KK1512
NACRES: NA.55

General description
KAPA Taq is supplied in a 2X ReadyMix™ format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.
Application
KAPA Taq HotStart® may be used in:
High throughput PCR
Amplification of low copy DNA templates
Multiplex PCR
Specific amplification of complex templates
RT-PCR
Polymerase chain reaction (PCR)

Biochem/physiol Actions
KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 53 polymerase and 53 exonuclease activities, but no 3 5 exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotStart buffer is a uniquely formulated buffer to facilitate specific primer annealing. This translates to higher yields of a specific product when compared to traditional Taq buffers, and improved amplification of GC- and AT-rich templates. However, KAPA Taq hotStart DNA polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.
Features and Benefits
High performance
Improved sensitivity, specificity, and yields.
Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

Quick Notes:
KAPA Taq HotStart® DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Quality
Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.
Other Notes
For Research Use Only. Not for use in diagnostic procedures.
Legal Information
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Параметры

Quality Level 100,

shelf life 18 mo.

feature dNTPs included: no, hotstart

packaging pkg of 2500 U

Торговая марка Roche

application(s) PCR: suitable

shipped in dry ice

storage temp. 20°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany WGK 1

Flash Point F does not flash

Flash Point C does not flash
Узнать цену Получить предложение
Подробнее

KAPA Taq PCR Kit

High throughput PCR
Amplification of low copy DNA templates
Multiplex PCR
Specific amplification of complex templates
RT-PCR
random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
Polymerase chain reaction (PCR)
Genotyping

Biochem/physiol Actions
KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart® DNA Polymerase have 53 polymerase and 53 exonuclease activities, but no 3 5 exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
Features and Benefits
High performance:

Improved sensitivity, specificity, and yields
Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:

KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
The final MgCl2 concentration may need to be optimized to account for differences in buffer formulation.
KAPA Taq Buffers contain MgCl2 at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Quality
Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Quality Level	100,
shelf life	≤18 mo.
feature	dNTPs included: no, hotstart: no
packaging	pkg of 250 U (KK1014), pkg of 2500 U (BK1000), pkg of 500 U (KK1015), pkg of 5000 U (BK1002)
Торговая марка	Roche
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	−20°C

KAPA2G Fast HotStart® Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to 1 day with conventional protocols. KAPA2G Fast Genotyping Mix (2X) is a ready-to-use master mix containing all components for fast PCR with stabilizers, two inert dyes except primers and template.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Fast HotStart Genotyping Mix

KAPA2G Fast HotStart® Genotyping Mix has been used in:

Genomic DNA extraction and in the preparation of master mix for PCR
Extraction and amplification from mouse tail, ear, and toe tissue
Extraction and amplification of DNA from other animal tissues

Biochem/physiol Actions
KAPA2G Fast Genotyping Mix generated DNA fragments are 3-dA-tailed and may be cloned into TA cloning vectors, or used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. Unlike existing protocols that rely on Proteinase K digestion, extractions using KAPA Express Extract are conveniently performed in a single tube, without the need for hazardous chemicals, or multiple wash steps.
Features and Benefits
Increased throughput, turnaround time and reliability:

PCR-ready DNA generated in as little as 15 minutes
Minimal handling, thereby reducing the risk of sample loss or contamination
Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format
Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents

Outperforms crude extraction methods and commercial kits:

Increased turnaround times
Increased specificity
Decreased cost

Quick Notes:

Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes
Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0-8.5), and use 1 µL of DNA per 25 µL reaction.
Extracts are stable at -20°C for >6 months.
KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl2 (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
Use 15 sec annealing time, with 15 sec extension time for amplicons <1 kb, or 15-30 sec/kb extension for longer amplicons.

Packaging

2X KAPA2G Fast Standard or HotStart Genotyping Mix with dye

Quality
All components contained with KAPA2G Fast HotStart Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities and meet strict requirements with respect to DNA contamination.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Quality Level	100,
shelf life	18 mo.
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart
packaging	kit of 1.25 mL (100 x 25 µL rxn; KK5620), kit of 6.25 mL (500 x 25 µL rxn; KK5621)
Торговая марка	Roche
application(s)	PCR: suitable, genotyping: suitable
input	crude DNA
shipped in	dry ice
storage temp.	20°C

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

pictograms	GHS08
signalword	Warning
hcodes	H371
pcodes	P260 - P264 - P270 - P308 + P311 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Fast HotStart PCR Kit

KAPA2G Fast HotStart® PCR Kit has been used in:

Routine PCR
Fast PCR
Genotyping

Biochem/physiol Actions
DNA fragments generated with KAPA2G Fast DNA Polymerase may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
Features and Benefits
Reduce cycling times up to 75% :

Extension times as low as 1 sec per kilobase
Broad coverage of both AT- and GC-rich targets

High speed, higher performance :

Single copy sensitivity from complex targets
Increased yields

Quick Notes:

KAPA2G Fast HotStart PCR Kits with dNTPs & Mg-free buffers contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
No need for specialized instrumentation or PCR consumables.
Optimized buffer system offers improved yields,specificity and sensitivity, facilitating efficient primerannealing across a wide range of primer lengths,GC contents, and melting temperatures.
Use 0.5 U KAPA2G Fast HotStart DNA Polymerase per 25 µL reaction, or less for smaller volumes.
For high reaction efficiency, do not exceed 25 µL reaction volumes.
The buffer is available with and without MgCl2 KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X, while KAPA2G Buffer M is Mg-free. Aside from MgCl2 content, these two buffers are identical.
Reaction products are 3-dA-tailed and may be cloned into TA cloning vectors.

Quality
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the users own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Quality Level	100,
shelf life	18 mo.
feature	Fast PCR, dNTPs included, hotstart
packaging	pkg of 250 U (KK5512), pkg of 500 U (KK5510)
Торговая марка	Roche
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	20°C

KAPA2G Fast HotStart® ReadyMix™ has been used for:

pictograms	GHS07,GHS08
signalword	Warning
hcodes	H302 - H371 - H412
pcodes	P260 - P264 - P270 - P273 - P308 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Fast HotStart ReadyMix

Routine PCR
Fast PCR
Reverse transcription polymerase chain reaction (RT-PCR)
Genotyping and fluorescent PCR

Biochem/physiol Actions
KAPA2G Fast HotStart® DNA Polymerase generated DNA fragments may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Fast has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
Features and Benefits
Reduce cycling times up to 75% :

Extension times as low as 1 sec per kilobase
Broad coverage of both AT- and GC-rich targets

High speed, higher performance :

Single copy sensitivity from complex targets
Increased yields

Quick Notes :

KAPA2G Fast HotStart ReadyMix PCR Kits contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
No need for specialized instrumentation or PCR consumables.
Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
For high reaction efficiency, do not exceed 25 µL reaction volumes.
KAPA2G Fast HotStart ReadyMix contains 1.5 mM MgCl2 at 1X.
KAPA2G Fast HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.Reaction products are 3-dA-tailed and may be cloned into TA cloning vectors.

Quality
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the users own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	100,
shelf life	18 mo.
feature	Fast PCR, Multiplex PCR, dNTPs included, hotstart
packaging	kit of 1.25 mL (100 x 25 µL rxn; KK5603), kit of 6.25 mL (500 x 25 µL rxn; KK5601)
Торговая марка	Roche
concentration	2 x
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	20°C

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast ReadyMix™ PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

pictograms	GHS08
signalword	Warning
hcodes	H371
pcodes	P260 - P264 - P270 - P308 + P311 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Fast ReadyMix

KAPA2G Fast ReadyMix™ has been used in

Nested PCR (nPCR)
Routine PCR
Fast PCR
Genotyping

Biochem/physiol Actions
DNA fragments generated with KAPA2G Fast DNA Polymerase may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides. Like wild-type Taq, KAPA2G Fast has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity.
Features and Benefits
Reduce cycling times up to 75%:

Extension times as low as 1 sec per kilobase
Broad coverage of both AT- and GC-rich targets

High speed, higher performance:

Single copy sensitivity from complex targets
Increased yields

Quick Notes:

KAPA2G Fast ReadyMix PCR Kits contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
No need for specialized instrumentation or PCR consumables.
Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primerannealing across a wide range of primer lengths, GC contents, and melting temperatures.
For high reaction efficiency, do not exceed 25 µL reaction volumes.
KAPA2G Fast ReadyMix contains 1.5 mM MgCl2 at 1X.KAPA2G Fast ReadyMix includes two inert tracking dyes to allow direct loading onto agarose gels.
Reaction products are 3-dA-tailed and may be cloned into TA cloning vectors.

Quality
Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- andendonuclease activity, and meets strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the ReadyMix hasbeen handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally )at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°Cis not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradationwhen contaminated during use, and therefore storage at such temperatures is at the users own risk.

For Research Use Only. Not for use in diagnostic procedures.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Quality Level	100,
shelf life	≤18 mo.
feature	Fast PCR, dNTPs included, hotstart: no
packaging	kit of 1.25 mL (100 x 25 μL rxn; KK5101), kit of 25 mL (KK5103), kit of 6.25 mL (500 x 25 μL rxn; KK5102)
Торговая марка	Roche
concentration	2 x
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	−20°C

The second-generation KAPA2G Robust DNA Polymerase evolves to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). This product enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

pictograms	GHS08
signalword	Warning
hcodes	H371
pcodes	P260 - P264 - P270 - P308 + P311 - P405 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Robust HotStart PCR Kit

KAPA2G Robust HotStart® PCR Kit has been used for:

Amplification of GC- or AT-rich templates
Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
Colony PCR
qPCR
Nested polymerase chain reaction (PCR) amplification
Amplification of DNA targets in PCR
DNA amplification using conventional PCR
Routine PCR

Biochem/physiol Actions
DNA fragments created with KAPA2G Robust HotStart® DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase. It can be used for routine downstream applications, such as restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 53 polymerase and 53 exonuclease activities, devoid of 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Robust HotStart is similar to wild-type Taq. It shows an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.
Features and Benefits
Efficient amplification of GC- and AT-rich targets

Robust performance across a wide range of GC- and AT-rich templates
Increased PCR success rates

Improved tolerance to common PCR inhibitors

Efficient amplification from crude samples
Higher yield and sensitivity per unit of enzyme

Unrivalled performance in colony PCR

Higher yields and improved consistency of PCR direct from E. coli and yeast cells

Quick Notes:

KAPA2G Robust HotStart® PCR Kits with dNTPs contain KAPA2G Robust HotStart DNA Polymerase, engineered for high processivity and inhibitor tolerance.
Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template.
Use 0.5 U of KAPA2G Robust HotStart DNA Polymerase per 25 µL reaction. More challenging PCRs (GCrich,crude sample) may require higher enzyme concentrations.
Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.
KAPA2G Buffers contain 1.5 mM MgCl2 at 1X.
Additional MgCl2 may be added using the 25 mM MgCl2 solution provided in the kit.
KAPA2G Buffer A is optimized for high yield, specificity, and sensitivity.
KAPA2G Buffer B is recommended for inhibitorcontaminated template samples.
KAPA2G GC Buffer is recommended for GC-rich PCR (>70% GC).
KAPA Enhancer 1 can be used with KAPA2G Buffer A or B, particularly for GC-rich assays.
Reaction products are 3-dA-tailed and may be cloned into TA cloning vectors.

Quality
Each batch of KAPA2G Robust HotStart® DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Quality Level	100,
shelf life	≤12 mo.
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart
packaging	pkg of 100 U (KK5532), pkg of 250 U (KK5516), pkg of 500 U (KK5518)
Торговая марка	Roche
application(s)	PCR: suitable
input	crude DNA
shipped in	dry ice
storage temp.	−20°C

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). KAPA2G Robust HotStart® ReadyMix™ enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

pictograms	GHS07,GHS08
signalword	Warning
hcodes	H302 - H371 - H412
pcodes	P260 - P264 - P270 - P273 - P308 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA2G Robust HotStart ReadyMix

KAPA2G Robust HotStart® ReadyMix™ has been used for the:

amplification of GC- or AT-rich templates
aplification of templates containing common polymerase chain reaction (PCR) inhibitors e.g. salts, urea, SDS, or ethanol
crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
colony PCR
DNA extraction and amplification
mutation screening
methylation-specific PCR (MSP) amplification
genotyping-PCR
PCR-based sequencing

Biochem/physiol Actions
DNA fragments generated with KAPA2G Robust HotStart® DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 53 polymerase and 53 exonuclease activities, but no 35 exonuclease (proofreading) activity. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated by KAPA2G Robust HotStart are 3-dA-tailed and may be cloned into TA cloning vectors.
Features and Benefits
Efficient amplification of GC- and AT-rich targets

Robust performance across a wide range of GC- and AT-rich templates
Increased PCR success rates

Improved tolerance to common PCR inhibitors

Efficient amplification from crude samples
Higher yield and sensitivity per unit of enzyme

Unrivalled performance in colony PCR

Higher yields and improved consistency of PCR direct from E. coli and yeast cells

Quick Notes:

KAPA2G Robust HotStart ReadyMix PCR Kits contain KAPA2G Robust DNA Polymerase, engineered for high processivity and inhibitor tolerance.
Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template.
Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.
KAPA2G Robust HotStart ReadyMix contains 2 mM MgCl2 at 1X.
KAPA2G Robust HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.
KAPA2G Robust HotStart ReadyMix is ideal for single-protocol PCR, i.e. amplification of fragments varying from 25–65% GC, up to 1 kb in size, using a single reaction setup and cycling protocol.
Reaction products are 3-dA-tailed and may be cloned into TA cloning vectors.

Quality
Each batch of KAPA2G Robust HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Robust HotStart ReadyMix PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

For Research Use Only. Not for use in diagnostic procedures.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

shelf life	12 mo.
Quality Level	100,
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart
packaging	kit of 1.25 mL (100 x 25 µL rxn; KK5701), kit of 6.25 mL (500 x 25 µL rxn; KK5702)
Торговая марка	Roche
concentration	2 x ( contains 2 mM MgCl₂ at 1x)
application(s)	PCR: suitable
input	crude DNA
shipped in	dry ice
storage temp.	20°C

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.
KAPA3G Plant DNA Polymerase is a blend of the engineered A-family KAPA3G DNA Polymerase and a modified B-family DNA Polymerase. The enzyme blend is combined with proprietary antibodies to inactivate the enzymes prior to the first denaturation step. The fidelity of the KAPA3G Plant DNA Polymerase is 4-10 times higher than that of wild-type Taq.

pictograms	GHS07,GHS08
signalword	Warning
hcodes	H302 - H371
pcodes	P260 - P264 - P270 - P301 + P312 + P330 - P308 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

KAPA3G Plant PCR Kit

KAPA3G Plant PCR Kit has been used for:

Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits or cetyl trimethyl ammonium bromide (CTAB)-based methods
Direct polymerase chain reaction (PCR) from leaf discs, seed samples, and other plant tissue types
PCR from crude plant DNA extracts, prepared from leaf and/or seed material
amplification of 16SrRNA
an ultra-rapid real-time reverse transcriptase (RT)-PCR assay

Features and Benefits
Key features of the KAPA3G Plant PCR Kit include:

Fast PCR direct from plant tissues such as leaf discs, seeds and crude plant extracts.
Streamlined workflows for transgenic screening.
Improved PCR success rates and reproducibility.
Efficient amplification of long and difficult targets from all sample types.

Amplify long targets from crude samples and purified DNA:

Amplify fragments up to 5 kb from purified DNA and crude samples
High yield and specificity with purified DNA and crude samples

Perform direct PCR from a variety of plant species and tissue types:

Direct PCR with leaf disc or seed as template
No need for time-consuming DNA extractions

Streamline workflows and improve turnaround times:

Perform PCR in half the time compared with wild-type enzymes
Eliminate the need for time-consuming

DNA extractions Improve success rates with novel crude sample plant PCR workflow:

Use extraction buffer to prepare crude extracts for plant PCR in just 5 minutes
High success rates with even the most challenging sample types

Quick Notes:

KAPA3G Plant DNA Polymerase is tolerant to plant-derived PCR inhibitors, and can amplify frompurified DNA, crude extracts, and plant material.
Optimize reaction conditions using purified DNA before attempting direct or crude sample PCR.
For direct PCR, use a sampling tool to control the amount of plant material added to the reaction. The use of excessive amounts of crude plant material in PCR is a major cause of direct PCR reaction failure.
For crude sample PCR, prepare a crude DNA extract using a small amount of plant material in Extraction Buffer (Refer to Section 3: Crude sample PCR), and use 1 µL per 50 µL reaction.
KAPA Plant PCR Buffer contains MgCl2 (1.5 mM at 1X) and dNTPs (0.2 mM each at 1X). Additional MgCl2(25 mM) is supplied separately for optimization.

Quality
Each batch of KAPA3G Plant DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA3G Plant PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.
Preparation Note
Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

For Research Use Only. Not for use in diagnostic procedures.

Quality Level	100,
usage	50 μL sufficient for 250 reactions (KK7251), 50 μL sufficient for 500 reactions (KK7252)
shelf life	≤12 mo.
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included: no, hotstart: no
Торговая марка	Roche
application(s)	PCR: suitable
input	crude DNA
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

PCR Master 11636103001

The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl2. PCR products generated by PCR Master are 3-A single-overhang products. Therefore T/A cloning can be applied.
The PCR Master is stable at +2 to +8 °C, allowing immediate PCR without the time-consuming thawing of reagents.
Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9. The 5 3 DNA polymerase lacks 3 5 exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.

The PCR Master can be used instead of the single reagent Taq DNA Polmerase, or instead of the PCR Core Kit for nearly all simple, routine polymerase chain reaction applications. The only limitation is that the sample volume must not exceed half the total reaction volume. The PCR master mix is used for:

Routine PCR
RT-PCR
Other primer-extension reactions, such as sequencing and labeling

High lot-to-lot consistency.

Reliable reproducible results:

Convenience:

Adding template and primer requires only two pipetting steps.

Prevent PCR carryover:

dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

Ready for immediate use:

Master mix can be stored at +2 to +8°C.
Packaging
1 kit containing 2 components.
Quality
Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

usage	sufficient for 200 reactions, (50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase)
Quality Level	100,
feature	dNTPs included, hotstart: no
Торговая марка	Roche
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	−20°C

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

PCR Nucleotide Mix

Features and Benefits
The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance

The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
RT-PCR
cDNA synthesis
Primer extension

Choose a convenient ready-to-use mix of all 4 nucleotides.
Achieve the highest PCR and RT-PCR sensitivity.
Insist on highest purity (>99%) nucleotides.

Packaging
1 set containing 4 ready-to-use mixes
Quality
Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.
Preparation Note
Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 2,500 reactions (04638956001), sufficient for 5,000 reactions (11814362001), sufficient for 500 reactions (11581295001)
packaging	pkg of 10 x 200 μL (11814362001 [10mM, each]), pkg of 200 μL (11581295001 [10 mM, each]), pkg of 5 x 200 μL (04638956001 [10 mM, each])
Торговая марка	Roche
shipped in	dry ice
storage temp.	−20°C

Protector RNase Inhibitor inactivates a wide spectrum of RNases, including

Узнать цену

Подробнее

Protector RNase Inhibitor

RNase A
RNase B
RNase T2

Thus, Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems. For instance, it can:

protect mRNA during cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), or in vitro transcription/translation reactions
protect viral RNA during in vitro virus replication
inhibit RNases during RNA isolation and purification
be used in RNase protection assays
help prepare RNase-free antibodies

Note: Protector RNase Inhibitor does not interfere with enzymes commonly used to prepare or analyze RNA.
Contents
Protector RNase Inhibitor (40 U/µl) in storage buffer: 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol (v/v), pH approximately 7.6 (+4°C).

Specificity

Protector RNase Inhibitor definetely does not inhibit RNase T1 and RNase 1.
Protector RNase Inhibitor (20 U/ 20 µl reaction) inhibits RNase A up to 1 ng, RNase B up to 160 pg and RNase T2 up to 0.03 U/µl reaction volume.
RNase H is not inhibited by Protector RNase Inhibitor.
Heat inactivation: > 65 °C

Protector RNase Inhibitor may be used to:

Protect mRNA in cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), e.g., with the LightCycler® Instruments, in vitro transcription/translation system, RNase Protection Assay, in vitro RNA synthesis
Protect viral RNA during in vitro virus replication
Inhibit RNases during RNA isolation and purification
Help prepare RNase-free antibodies
Synthesize RNA probes for in situ hybridization

It is useful in any application where RNases could be a potential problem.

Each lot of Protector RNase Inhibitor is function tested with the Titan One Tube RT-PCR Kit and the LightCycler DPD Kit. Protector RNase Inhibitor is also tested for contaminating activities as described below.
Test buffer: 50 mM Tris-HCl, 10 mM MgCl2, 0.1 mM EDTA, 7 mM -Mercaptoethanol; pH 7.5 (+37°C).
Absence of endonucleases: 1 µg EcoR I/Hind III*-fragments of DNA is incubated with Protector RNase Inhibitor in 50 µl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no alteration in the banding pattern is stated under "Endo".
Absence of nicking activity: 1 µg supercoiled pBR322 DNA is incubated with Protector RNase Inhibitor in 50 µl test buffer at +37°C for 1 hour. The amount of inhibitor that causes no relaxation of supercoiled DNA is stated under "Nick. Act.".
Absence of ribonuclease (1): 5 µg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C in a final volume of 50 µl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 1".
Absence of ribonuclease (2): 5 µg of MS2 RNA is incubated with Protector RNase Inhibitor for 1 hour at +37°C, then 10 minutes at +65°C in a final volume of 50 µl. The amount of inhibitor that causes no degradation of MS2 RNA is stated under "RNase 2".

Rely on a thermostable RNase inhibitor: Protector RNase Inhibitor remains stable even when using thermostable reverse transcriptases such as Transcriptor Reverse Transcriptase.
Benefit from a wide range of reaction conditions: Protector RNase Inhibitor is active at pH 5.0 to 9.0 and at temperatures between +25°C to +55°C (partial activity is still measureable at +60°C).
Eliminate interference in different RNA analysis applications: Maintain performance even when adding at 16-fold higher than the standard concentration.
Insist on a highly-purified preparation: Each batch is function tested using techniques such as quantitative RT-PCR to ensure the absence of endonucleases, ribonucleases, or nicking activity, according to the current quality control procedures.

Quality

Unit Definition

One unit of Protector RNase Inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5 ng RNase A.
Unit Assay: Activity is measured according to Blackburn as ability to inhibit hydrolysis of cyclic cytidine-2 : 3-monophosphoric acid. Under assay conditions, 200 U of Protector RNase Inhibitor inhibits 50% of the activity of 1 µg RNase A.
Volume Activity: 40 U/µl

Preparation Note

Working concentration:

5 to 10 U in One-step RT-PCR
25 to 50 U in Two step RT-PCR
20 U for in vitro transcription

You may use higher concentrations of Protector RNase Inhibitor in RT-PCR if you suspect that RNase contamination causes certain samples to be difficult to amplify. The inhibitor does not interfere with the reaction. (In a test system, even a 16-fold higher concentration
of inhibitor did not interfere with RT-PCR.)

Product Specifications:
Source: Rat lung; Product is produced recombinant in E. coli
Isoelectric Point: pH 4.5
Temperature where Protector RNase Inhibitor is active: 25°C to +55°C; partial activity is still measurable at +60°C
Inactivation: Under severe denaturizing conditions (temperatures above +65°C), the inhibitor is inactivated
Bioburden: <50 cfu/ml
DNA Content: <100 pg/mg
For life science research only. Not for use in diagnostic procedures.

NOTICE TO PURCHASER: DISCLAIMER OF LICENSE
This product is optimized for use in the polymerase chain reaction (PCR) covered by patents owned by F. Hoffmann-La Roche Ltd ("Roche"). No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser by the purchase of this product. A license to use the PCR Process for certain research and development activities accompanies the purchase of certain Roche, Applied Biosystems or other licensed suppliers reagents when used in conjunction with an authorized thermal cycler, or is available from Applied Biosystems. Diagnostic purposes require a license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
LightCycler is a registered trademark of Roche

Quality Level	100
assay	>95% (SDS-PAGE)
mol wt	~50 kDa
packaging	pkg of 10,000 U (03335402001 5 x 2,000 U), pkg of 2,000 U (03335399001)
mfr. no.	Roche
parameter	25-55 °C optimum reaction temp.
pH range	5.0 - 9.0
shipped in	dry ice
storage temp.	−20°C

Узнать цену

Подробнее

Pwo DNA Polymerase

Pwo DNA Polymerase is a highly processive thermostable 5′-3′ DNA polymerase and possesses a 3′–5′ exonuclease activity, also known as proofreading activity. The enzyme has no detectable 5′–3′ exonuclease activity. Pwo DNA Polymerase exhibits increased thermal stability with a half-life of more than two hours at +100 °C, compared to Taq DNA Polymerase′s half-life of less than 5 minutes at this temperature. Pwo DNA Polymerase-generated PCR products are blunt-ended and can therefore be used directly for blunt-end ligation without any pretreatment of the ends.

Due to its proofreading activity, the thermostable Pwo DNA Polymerase has an extremely low error rate, 18-fold lower compared to Taq DNA Polymerase. It is therefore ideal for applications that require the highest possible fidelity in DNA synthesis. It can be applied for High fidelity PCR
Cloning of PCR products
Characterization of rare mutations
PCR

Packaging
1 kit containing 4 components
Quality
Each lot of Pwo DNA polymerase is assayed for:

Excellent accuracy (18-fold more accurate than Taq DNA polymerase)
High thermal stability
Nearly as processive as Taq DNA polymerase
Accepts modified nucleotides

Activity: The enzyme is tested on activated DNA.
Function: The enzyme is tested in two PCRs, using DNA and human genomic DNA as templates.
Proofreading ability: Proofreading activity is assayed according to the laq Iq fidelity assay [Frey, B. & Suppmann, B. (1995) Biochemica 2. 8-9].
Absence of nucleases: The enzyme is tested on various substrates to ensure the absence of detectable endonucleases, exonucleases, and nicking activity according to the current Quality Control procedures.

Unit Definition
One unit Pwo DNA polymerase is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol total deoxynucleoside triphosphates into acid precipitable DNA within 30 minutes at +70 °C under the conditions described below.
Unit Assay: Incubation buffer for assay on activated DNA

20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [-32P]dCTP are incubated with 0.01 to 0.1 U Pwo DNA Polymerase in 50 µl incubation buffer with a paraffin oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/µl
Preparation Note
Pwo DNA polymerase was originally isolated from Pyrococcus woesei, a hyperthermophilic archaebacterium.
Modified nucleotides are substrates
Pwo DNA Polymerase accepts modified nucleotides like digoxigenin-dUTP, biotin-dUTP, or fluorescein-dUTP. Thus, it can add these nucleotides to DNA during PCR. These nonradioactively labeled products can be used as a hybridization probe in many applications.
Magnesium concentration
If you use the magnesium-containing reaction buffer supplied with the enzyme, the final MgCl2 concentration in the PCR will be 2.0mM. For other magnesium concentrations (e.g., for optimizing the reaction to accommodate a particular template), use the magnesium-free reaction buffer and add appropriate amounts of the magnesium stock.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤200 reactions (11644955001), sufficient for ≤40 reactions (11644947001)
Quality Level	100,
feature	High Fidelity PCR, dNTPs included: no, hotstart: no
packaging	pkg of 100 U (11644947001), pkg of 500 U (11644955001 [2 x 250 U])
Торговая марка	Roche
application(s)	PCR: suitable
shipped in	dry ice
storage temp.	−20°C

Узнать цену

Подробнее

Pwo SuperYield DNA Polymerase

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction without changing the fidelity of DNA synthesis. This enzyme delivers excellent results due to its enzyme design and optimized buffer system. Amplify fragments up to 3 kb - even longer amplicons are possible from simple templates.
Pwo SuperYield DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5 3 DNA polymerase with 3 5 exonuclease proofreading activity; 5 3 exonuclease activity is not detectable.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
Specificity
Star activity: In cases where star activity is observed and/or the activity of the enzyme in the PCR mix is low, first purify the amplification product prior to the restriction enzyme digest using High Pure PCR Product Purification Kit.

Features and Benefits
Pwo SuperYield DNA Polymerase yields more high fidelity PCR product. The optimized buffer delivers superior results. Amplify fragments up to 3kb. A GC-RICH Solution is included for difficult templates.

Pwo Super Yield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for: High fidelity PCR
Site-directed mutagenesis
Cloning
Gene expression
Study of allelic polymorphism in individual RNA transcripts
Characterization of rare mutations in tissue
Characterization of the allelic stage of single cells or single DNA molecules

Higher yield and 18-fold higher fidelity

compared to Taq DNA Polymerase.

High performance with difficult templates.

The GC-RICH Solution is for GC-rich PCR.

Reduce working steps in cloning.

Perform digests directly in Pwo SuperYield PCR mix.
Packaging
1 kit containing 3 components
Quality
Each lot is assayed using activated DNA. PCR testing used λ and human genomic DNA.
Unit Definition
One unit Pwo SuperYield DNA Polymerase is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol total deoxynucleoside triphosphates into acid precipitable DNA within 30 minutes at +70 °C.
Unit Assay: Incubation buffer for assay on activated DNA
20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [-32P]dCTP are incubated with 0.01 to 0.1 U Pwo SuperYield DNA Polymerase in 50 µl incubation buffer with a paraffin-oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/µl

Modified nucleotides are substrates
Pwo DNA Polymerase accepts modified nucleotides like digoxigenin-dUTP, biotin-dUTP, or fluorescein-dUTP. Thus, it can add these nucleotides to DNA during PCR. These nonradioactively labeled products can be used as a hybridization probe in many applications.
For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤200 reactions (04340850001), sufficient for ≤40 reactions (04340868001)
Quality Level	100,
feature	Difficult Templates/Specialty Enzymes PCR, dNTPs included: no, hotstart: no
packaging	pkg of 100 U (04340868001), pkg of 500 U (04340850001 [2 x 250 U])
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
shipped in	dry ice
storage temp.	−20°C

Узнать цену

Подробнее

Reverse Transcriptase AMV 10109118001

2.7.7.49 ( BRENDA | IUBMB )

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are , and . The molecular weight of the -subunit is 68 kDa, that of the -subunit 92 kDa. The mature form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3 end labeling of DNA fragments, and the generation of ss probes for genomic footprints.
Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.
Specificity
AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C

Reverse Transcriptase AMV is suitable for:

First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
Dideoxy DNA sequencing
Primer extension
RNA sequencing
3-end labeling of DNA fragments
Generation of single-stranded probes for genomic footprint experiments

Packaging
1 kit containing 2 components
Quality
Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.
Unit Definition
1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.
Volume Activity: 20-25 U/µl. Please refer to the Certificate of Analysis for more information.
Preparation Note
Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.
Storage and Stability
Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
Procure full length cDNA fragments up to 12 kb
Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
specific activity	>50 units/μg protein
feature	dNTPs included: no
packaging	pkg of 1,000 U
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
parameter	42 °C optimum reaction temp.
application(s)	RT-qPCR: suitable
input	purified RNA
detection method	probe-based
shipped in	dry ice

T4 Gene 32 Protein is reported to improve the yield of long PCR products when 0.5 to 1.0 nmol of the protein is included in the reaction. T4 Gene 32 Protein is also reported to increase the yield of PCR products amplified from samples that contain humic acid. The inhibitory effect of humic acid is reduced by a factor of 7 when T4 Gene 32 Protein is included in the PCR (at a concentration of 2.5 µg/100 µl).
The protein is isolated from the triple-mutant T4amN134/ amBL292/ amE 219 defective for the T4 genes 33, 35, and 58 to 61.
Specificity
T4 gene 32 protein is a single-strand specific, helix-destabilizing protein encoded by gene 32 of the phage T4 genome. It is required for the replication of DNA and genetic recombination in Escherichia coli cells that are infected with T4 bacteriophage.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

T4 Gene 32 Protein

T4 Gene 32 Protein is a single-strand-specific DNA-binding protein. It may be used for:

Optimization of PCR (See "Product Description" below.)
Stimulation of in vitro DNA synthesis
Stabilization of single-stranded regions of DNA or RNA
Site-specific mutagenesis experiments (with T4 DNA polymerase and T4 DNA ligase)
Helping restriction enzyme digestions go to completion
q-PCR

Quality
Absence of contaminants
T4 gene 32 protein is incubated with various nucleic acids that serve as potential nuclease substrates. Results are analyzed by gel electrophoresis. Based on these tests, none of the following are detectable in the preparation according to the current Quality Control procedures:

nonspecific exo- and endonucleases
single-strand DNases (nicking activities)
single-strand-specific exonucleases
RNases

Physical form
Protein solution, 1 - 10mg/ml, in storage buffer (20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 0.5mM DTT, 50% glycerol [v/v], pH approximately 8.0)
Preparation Note
Working concentration: 1 to 10 mg/ml

For life science research only. Not for use in diagnostic procedures.

biological source	Escherichia coli (infected with phage T4amN134/amBL292/amE218)
Quality Level	100,
form	solution
packaging	pkg of 100 µg (10972983001), pkg of 500 µg (10972991001)
Торговая марка	Roche
optimum pH	~8.0
shipped in	dry ice
storage temp.	20°C

Узнать цену

Подробнее

Taq DNA Polymerase (1 U/l), dNTPack

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.

Taq DNA Polymerase (1 U/µl), dNTPack may be used for:

PCR
RT-PCR
Other primer-extension reactions, such as sequencing and labeling

Packaging
1 kit containing 5 components
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 1 U/µl

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤2,000 reactions (04738241001), sufficient for ≤500 reactions (04738225001)
Quality Level	100,
feature	dNTPs included, hotstart: no
packaging	pkg of 1,000 U (04738241001 [4 x 250 U]), pkg of 250 U (04738225001)
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
optimum pH	~9.0 (20 °C)
shipped in	dry ice
storage temp.	−20°C

Узнать цену

Подробнее

Taq DNA Polymerase, dNTPack

Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.
Taq DNA Polymerase is a highly processive 53 DNA polymerase that lacks 35 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.
Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM, a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Contents
Taq DNA Polymerase, 5 U/µl
PCR Buffer, 10x concentrated, with MgCl2
PCR Nucleotide Mix
For maximum convenience, select the ready-to-use 2x concentrated PCR Master.

Achieve the most consistent results in simple, routine PCR by using Roche Applied Science Taq DNA Polymerase, which is held to our rigorous purity and quality standards. The Taq DNA Polymerase, dNTPack, combines our standard preparation (5 U/µl) of recombinant Taq DNA Polymerase with our convenient, ready-to-use PCR Nucleotide Mix. In all other respects, this preparation is identical to our standard preparation and can be used for:

PCR
RT-PCR
Other primer-extension reactions, such as sequencing and labeling

Packaging
1 kit containing 3 components
Quality
Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.
Unit Definition
One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.
Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/µl

Reliable reproducible results: High lot-to-lot consistency.
No need to test each lot: Taq DNA Polymerase is rigorously tested.
Prevent PCR carryover: dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.

For life science research only. Not for use in diagnostic procedures.
Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.

Quality Level	100,
description	Number of Reactions: If 1.25 U are used per 50 µL reaction, Taq DNA Polymerase, dNTPack is designed for approximately sufficient for number of reactions, mentioned in the usage.
usage	sufficient for 2,000 reactions (04728904001), sufficient for 4,000 reactions (04728858001), sufficient for 400 reactions (04728874001), sufficient for 80 reactions (04728866001), sufficient for 800 reactions (04728882001)
feature	dNTPs included, hotstart: no
packaging	pkg of 1,000 U (04728882001 [4 x 250 U]), pkg of 2,500 U (04728904001 [10 x 250 U]), pkg of 5,000 U (04728858001 [20 x 250 U]), pkg of 100 U (04728866001), pkg of 500 U (04728874001 [2 x 250 U])
Торговая марка	Roche
parameter	72 °C optimum reaction temp.
application(s)	PCR: suitable
optimum pH	~9.0 (20 °C)
shipped in	dry ice
storage temp.	20°C

Узнать цену

Подробнее

Titan One Tube RT-PCR Kit 11939823001

Titan™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.
The Titan One Tube RT-PCR Kit is designed for fast, sensitive and reproducible analysis of RNA by high fidelity reverse transcription polymerase chain reaction (RT-PCR) in a one-step reaction.
Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Titan One Tube RT-PCR Kit has been used:

to complete cDNA synthesis in reverse transcription-quantitative polymerase chain reaction (RT-qPCR)
to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1
to amplify full-length and truncated cDNA fragments of C-X-C motif chemokine 5 (CXCL5)
to analyze RNA (total RNA, poly (A)+ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,† then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods
for cloning of RNA up to 6 kb
for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques

Is faster than conventional two-step RT-PCR.
Packaging
1 kit containing 9 components

Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparison to Taq DNA polymerase.
Delivers increased sensitivity due to the high efficiency of all three enzymes.

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche

Quality Level	100,
usage	sufficient for 50 reactions
feature	dNTPs included: no, hotstart: no
Торговая марка	Roche
parameter	42-60 °C optimum reaction temp.
application(s)	RT-PCR: suitable
input	purified RNA
storage temp.	−20°C

pictograms	GHS07
signalword	Warning
hcodes	H319 - H412
pcodes	P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

Titan One Tube RT-PCR System 11855476001

The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:

Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
Delivers increased sensitivity due to the high efficiency of all three enzymes.
Is faster than conventional two-step RT-PCR.

Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Titan™ One Tube RT-PCR System has been used:

to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
for determining the presence or absence of RNA templates
in cloning of rare messages without the need to construct cDNA libraries
for quantifying the levels of gene expression

Features and Benefits
Titan™ One Tube RT-PCR System:

High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
offers a threefold higher fidelity in comparison to Taq DNA polymerase
delivers increased sensitivity due to the high efficiency of all three enzymes
faster than conventional two-step reverse transcriptase (RT)-PCR
allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System

Packaging
1 kit containing 4 components

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche

Quality Level	100,
usage	sufficient for 100 reactions
feature	dNTPs included: no, hotstart: no
Торговая марка	Roche
parameter	42-60 °C optimum reaction temp.
application(s)	RT-PCR: suitable
input	purified RNA
shipped in	dry ice
storage temp.	−20°C

In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66 kDa and 51 kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments.

pictograms	GHS07
signalword	Warning
hcodes	H319 - H412
pcodes	P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments) for the following applications:

Synthesis of first-strand cDNA for use in subsequent amplification reactions
RT-PCR of GC-rich RNA templates
Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
Retrieving and cloning the 5 and 3 termini of mRNA by RACE
Generation of cDNA libraries with large inserts
Dideoxy DNA sequencing
RNA sequencing
3-end labeling of DNA fragments
Generation of single-stranded probes for genomic footprints
In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)

Biochem/physiol Actions
Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.
Features and Benefits

Achieve high sensitivity in two-step RT-PCR.

Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler®Instruments).

Obtain more full-length transcripts - up to 14 kb.

cDNA libraries with large inserts can be generated.

Reverse transcribe difficult templates.

The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.

Efficiently label cDNA.

Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.
Components

Transcriptor Reverse Transcriptase, in storage buffer
Transcriptor RT buffer, 5x concentrated

Quality
Each lot of Transcriptor Reverse Transcriptase is routinely function tested in RT-PCR:

with a conventional thermal cycler to detect a 10-kb fragment from the human dystrophin gene, starting from human skeletal muscle total RNA
with the LightCycler® Instrument to detect 5 x 102 to 5 x 106 copies of in vitro-transcribed human PBGD RNA. The results are defined in fixed crossing points and fixed fluorescence intensity.

Preparation Note
Volume activity: 20 U/µl.
Transcription of long, rare, or difficult RNA targets
Transcriptor Reverse Transcriptase is recommended for RT-PCR of:

long targets, because it can transcribe up to 14 kb RNA templates
rare targets, because it has high sensitivity
GC-rich targets, because it can operate at high temperatures (up to +65oC) to eliminate problems associated with extensive secondary structure

Labeling for many applications
Transcriptor Reverse Transcriptase is also recommended for preparing labeled cDNA, since it accepts a wide variety of modified nucleotides (including Cy3-, Cy5-, DIG-, Biotin-, or aminoallyl-labeled dNTPs).
Reaction requirements
Transcriptor accepts ssRNA and ssDNA templates and requires a primer for transcription.

For life science research only. Not for use in diagnostic procedures.

LightCycler is a registered trademark of Roche

Quality Level	100,
Торговая марка	Roche
packaging	pkg of 200 reactions (03531287001), pkg of 25 reactions (03531317001), pkg of 50 reactions (03531295001)
application(s)	RT-qPCR: suitable
detection method	probe-based

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

Tth DNA Polymerase 11480022001

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5-3 DNA polymerase, and lacks 3-5 exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).

Thermus thermophilus (Tth) DNA Polymerase might be used:

to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
for real time PCR

Biochem/physiol Actions
Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5-3 DNA polymerase and lacks 3-5 exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb.
Features and Benefits
Tth DNA Polymerase:

Ensures optimized polymerase chain reaction (PCR) product size for at least up to 1,000 bp in a RT-PCR reaction
Accepts modified desoxyribonucleoside triphosphates as substrates
Has no association with RNase H activity
Has high thermostability to overcome the problem, typically associated with the high degree of secondary structure present in RNA

Packaging
1 kit containing 3 components
Quality
Each lot of Tth DNA Polymerase is assayed for activity on activated DNA. A function test is performed using ?DNA, as well as human genomic DNA. Performance in RT/PCR is assayed using total mouse RNA and primers specific for ?-actin. Each lot of Tth DNA Polymerase is tested for the absence of contaminating activities like exo- and endonucleases and nicking activities according to the current Quality Control procedures. A unique self-priming assay ensures the absence of contaminating DNA according to the current Quality Control procedures.
Unit Definition
One unit of Tth DNA Polymerase is defined as the amount of enzyme which catalyses the incorporation of 10 nmol total dNTPs into acid precipitable DNA within 30 minutes at +70 °C under the assay conditions stated in "unit assay".
Unit Assay: Incubation buffer for assay on activated DNA
67 mM Tris-HCI, pH 8.8 (+25 °C), 16.6 mM (NH4)2SO4, 6.7 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM dATP, dCTP, dGTP, dTTP each.
Incubation procedure
12.5 µg activated herring sperm DNA and 0.1 µCi [32P] dCTP are incubated with 0.01 to 0.1 units Tth DNA Polymerase in 50 µl Incubation buffer with a paraffin oil overlay at +70 °C for 30 min.
The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 0.5 to 5 U per reaction of PCR (optimal)
2.5 U per reaction of PCR (standard)
Determined in the assay on activated DNA described under "unit assay".

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for ≤200 reactions
Quality Level	100,
packaging	pkg of 500 U (2 x 250_U)
Торговая марка	Roche
parameter	75 °C optimum reaction temp.
optimum pH	~9.0 (25 °C)
shipped in	dry ice
storage temp.	−20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

Universal ProbeLibrary

Кат. номер
4684974001
4685059001
4685067001
4685075001
4685091001
4685008001
4685016001
4685024001
4685032001
4684982001

Each Universal ProbeLibrary probe is a single hydrolysis probe that recognizes sequences from one or more of the following organisms: humans, chimpanzees and other primates, mice, rats, C. elegans, Drosophila, Arabidopsis.
Short hydrolysis probes for quantification of gene expression levels by real-time PCR.

Use the Universal ProbeLibrary Probes to obtain rapid, flexible quantification of expression levels for virtually any gene from the human, mouse, rat, chimpanzee, C. elegans, Drosophila, or Arabidopsis transcriptomes through qRT-PCR or Real-time RT-PCR in the NCBI Reference Sequence Database. Each probe can be used with standard reagents in real-time PCR assays:

on the LightCycler® Carousel-Based System (e.g., with the LightCycler® TaqMan® Master), or
on other commercially available real-time PCR instruments (e.g., with the FastStart TaqMan® Probe Master).

Note: You can easily design gene-specific quantification assays using these probes and the free web-based ProbeFinder software at the Assay Design Center.
Features and Benefits
Each of the Universal ProbeLibrary probes:

Significantly reduces assay design time and implementation time.
Includes features that make probe-based assays specific, flexible, and convenient.
Needs no special hardware or unusual reaction conditions.

Components
Two vials, each containing 125 μL of one probe solution [10 μM solution of a single probe, 5′ labeled with fluorescein (FAM) and 3′ labeled with a dark quencher dye].
Quality
Function test: All Universal ProbeLibrary probes are tested for performance in real-time PCR using primers and control templates from the Universal ProbeLibrary Control Set. Each probe must pass this test.
Purity: Each probe is analyzed by anion-exchange HPLC.
Composition: Each probe is analyzed by MALDI-MS.

Probe chemistry: Universal ProbeLibrary probes use a unique nucleotide chemistry called LNA (Locked Nucleic Acid), which allows very short (8-9 bases) oligonucleotides to be effective hybridization probes in real-time PCR assays. LNA allows the melting temperature of the short probes to be unusually high. Thus, they are fully compatible with commonly used PCR conditions and the hydrolysis-probe detection format. These probes are even sensitive enough to detect single-base mismatches.
Assay design: The free online ProbeFinder software, available at the Universal ProbeLibrary Assay Design Center,, designs one or more intron-spanning assays for a target gene, based on specific information (i.e., gene name, accession number, or sequence) that you submit. For each assay it designs, the software will specify a set of specific primers plus a probe from the Universal ProbeLibrary that will give the best results. The combination of primers and probe will provide specific amplification and detection of your target sequence in a standard real-time PCR assay.
Labeling: pre-labeled with a reporter fluorophore (FAM) and a dark quencher dye.
Instrumentation required: standard real-time PCR instrumentation.
Notes: In addition to being available individually, the Universal ProbeLibrary probes are available in sets (90 probes/set), each of which covers virtually all the transcripts from a single organism. Together, the sets cover approximately 99% of the human, primate, mouse, rat, C. elegans, Drosophila, and Arabidopsis gene transcripts listed in the NCBI Reference Sequence Database.
For more information, see the listings for these organism-specific sets.
For life science research only. Not for use in diagnostic procedures.
Universal ProbeLibrary System Performance Data,
Universal ProbeLibrary System User Statements and Application,
Universal ProbeLibrary System Technology,

LightCycler is a registered trademark of Roche
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Quality Level	100,
Торговая марка	Roche
packaging	pkg of Probe #1 (04684974001), pkg of Probe #10 (04685091001), pkg of Probe #2 (04684982001), pkg of Probe #3 (04685008001), pkg of Probe #4 (04685016001), pkg of Probe #5 (04685024001), pkg of Probe #6 (04685032001), pkg of Probe #7 (04685059001), pkg of Probe #8 (04685067001), pkg of Probe #9 (04685075001)

RIDADR	NONH for all modes of transport
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Amylase 10102814001

3.2.1.1 ( BRENDA | IUBMB )

α-Amylase or 1,4-α-D-glucan-glucanohydrolase is a family of starch converting endoamylase enzymes. Endoamylases cleave at the endo or internal α,1-4 glycosidic bonds present in amylose and amylopectin. This reaction results in variable length oligosaccharides such as α-dextrins, which are branched oligosaccharides.
Specificity
Hydrolyzes α1,4-glucan bonds in polysaccharides with three or more α1,4-bound glucose units.

-amylase is used for the hydrolyzation of 1,4-glycosidic bonds in polysaccharides containing 3 or more 1,4-linked D-Glucose units. The enzyme produces -dextrins.
Packaging
50mg (5 mL)
Unit Definition
Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 µCi (32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Preparation Note
Activator: NaCI (10 mM) activates the pancreatic enzyme.
Stabilizers: Both, the bacterial and the pancreatic enzymes are stabilized by Ca2+ (bound Ca2+ is required for activity).
Working concentration: 2.5 to 5 mg/ml
Working solution: Phosphate buffer (100 mmol/l; pH 7.0).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~1000 units/mg protein (At 25 °C with soluble starch as the substrate, standardized with BSA.)
mol wt	M_r 50 kDa
packaging	pkg of 5 mL
Торговая марка	Roche
optimum pH	7
shipped in	wet ice

pictograms	GHS08
signalword	Danger
hcodes	H334
pcodes	P261 - P284 - P304 + P340 - P342 + P311 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Gal Reporter Gene Assay, chemiluminescent 11758241001

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.

The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Features and Benefits

Sensitive: Chemiluminescence technology allows detection of approximately 20 fg -galactosidase in cell extracts
High dynamic measuring range: Linear range over four to five orders of magnitude
Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
Safe: No radioactive isotopes are used
Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Packaging
1 kit containing 7 components.
Preparation Note
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 250 assays (tubes), sufficient for 500 assays (microplate)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS02,GHS05,GHS07
signalword	Danger
hcodes	H225 - H290 - H314 - H317 - H336 - H412
pcodes	P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
Target Organs	Central nervous system
RIDADR	UN 3316 9
WGK Germany	WGK 2

Узнать цену

Подробнее

-Gal Staining Set 11828673001

The -Gal Staining Set detects bacterial -Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a -Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol).
For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial -galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of -Gal as described for transfected cells.
Specificity
Bacterial β-galactosidase

The β-Gal Staining Set is used for the histochemical staining of cells and tissue sections demonstrating β-galactosidase activity.
β-Gal staining set has been used in immunofluorescence analysis, senescence assay and histopathologic analysis of tissue samples.
Packaging
1 set containing 2 components.
Specifications
Stability: Stable at +2 to +8°C

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 100 tests (in 3.5 cm dishes)
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H317
pcodes	P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Galactose Dehydrogenase

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase
Specificity
Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galactose [galactosamine], (relative rate = 0.02; pH 8.6).
Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32).
Quality
Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH
Sequence
Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D).
Unit Definition
One unit galactose dehydrogenase will oxidize 1 mol of -D-galactose in one minute at +25 °C and pH 8.6.
The QC assay produces 1 mol of NADH per mol of galactose oxidized.
Physical form
Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.)
packaging	pkg of 1 mL (10104973001 [1 mg]), pkg of 1 mL (10104981001 [5 mg])
Торговая марка	Roche
optimum pH	8.4 (0.1 M in Tris buffer)
pH range	4.6 - 9.2
shipped in	wet ice
storage temp.	2-8°C

Узнать цену

Подробнее

-Galactose Dehydrogenase S 10662046001

1.1.1.48 ( BRENDA | IUBMB )

D-galactose:NAD+ 1-oxidoreductase

β-Galactose Dehydrogenase S has been used in the colorimetric microassay method to determine the level of galactose and galactose-1-phosphate in blood.
Quality
Contaminants: <0.01% ADH, <0.01% -galactosidase, <0.1% LDH, <0.05% NADH oxidase
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	80 U/mg (Approximately 80 U/mg protein at +25°C with galactose as the substrate.), ~80 units/mg protein (At 25 °C with galactose as the substrate.)
packaging	pkg of 50 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

-Galactosidase 10105031001

3.2.1.23 ( BRENDA | IUBMB )

-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.
Specificity
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.

β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays.
Quality
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
Physical form
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
Preparation Note
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of -galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM -mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl--D-galactopyranoside.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~300 units/mg protein (At 37 °C with 2-nitrophenyl--D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.)
mol wt	540 kDa
packaging	pkg of 1,500 U
Торговая марка	Roche
optimum pH	7
shipped in	wet ice
storage temp.	2-8°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	No data available
Flash Point C	No data available

Узнать цену

Подробнее

2-Macroglobulin 10602442001

α2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.

α2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.
Biochem/physiol Actions
α2-Macroglobulin is a plasma endopeptidase and glycoprotein which functions on proteinases, especially serine proteases. It interacts with and inhibits endopeptidases. However, it does not act on other proteases, including inactive proteinases.
α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.
Sequence
2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.
Preparation Note
Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use 2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. 2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)
Analysis Note
Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of 2-macroglobulin is recommended.

For life science research only. Not for use in diagnostic procedures.

biological source	bovine plasma
Quality Level	100
form	lyophilized (Contains 6% BSA)
packaging	pkg of 25 IU
Торговая марка	Roche
application(s)	blocking: suitable
pH range	4 - 7
UniProt accession no.	Q7SIH1,
shipped in	wet ice
Gene Information	bovine ... A2M(513856)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Узнать цену

Подробнее

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

1.1.1.30 ( BRENDA | IUBMB )

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
Quality
Contaminants: <0.1% LDH, <5% MDH
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Stabilizers: NADH, Ca2+

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	suspension
specific activity	~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
packaging	vial of 2 mL (10127833001), vial of 5 mL (10127841001)
Торговая марка	Roche
concentration	5 mg/mL
impurities	<0.1% LDH, <5% MDH
optimum pH	6.2-6.9(for reduction), 7-9(for oxidation)
shipped in	wet ice
storage temp.	2-8°C