Roche® Life Science Products

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Labeling efficiency: Approximately 10µg of full-length digoxigenin-labeled RNA is transcribed from 1µg linear template DNA.
Assay Time: 135 minutes
Sample Materials
Linearized plasmid DNA:
The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of ‘run off transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5-overhangs should be used; 3 overhangs should be avoided. The linearized template DNA should be purified by phenol chloroform extraction and ethanol precipitation, to avoid RNase contamination. For run around transcription circular plasmid DNA is used.
PCR product:
PCR-fragments which contain RNA polymerase promoter sequences can also be used as templates for transcription. Purification of the PCR fragment by HighPure column purification prior to transcription is recommended.
DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. DIG-11-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The DIG RNA Labeling Mix is specifically designed for the use with SP6, T7, and T3 RNA polymerases, which are supplied with an optimized transcription buffer.
Convenient nucleotide mixture for the labeling of RNA with Digoxigenin-11-UTP.
Contents
10x solution with: 10 mM ATP, CTP, GTP (each), 6.5 mM UTP, 3.5 mM DIG-11-UTP.
Specificity
Heat inactivation: Stop the reaction by adding 2 µl 0.2 M EDTA (pH 8.0).

RNA labeling with Digoxigenin-11-UTP by in vitro transcription with SP6, T7, and T3 RNA polymerases. DIG-labeled RNA is used in a variety of hybridization techniques:

Northern blots
Southern blots
Dot blots
Plaque or colony lifts
RNase protection experiments
Chromosomes, cells, and tissue sections in situ

Features and Benefits
The DIG RNA Labeling Mix is especially designed for the use with SP6,T7 and T3 RNA polymerases from Roche which are supplied with an optimized transcription buffer.
Quality
Function tested in the DIG RNA Labeling Kit and in the DIG Nucleic Acid Detection Kit.

For life science research only. Not for use in diagnostic procedures.

form	solution
Quality Level	100,
usage	sufficient for 20 reactions
packaging	pkg of 40 µL
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS07
signalword	Warning
hcodes	H302
pcodes	P264 - P270 - P301 + P312 + P330 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DIG Wash and Block Buffer Set 11585762001

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.
Packaging
1 set containing 4 components.
Quality
The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.
Preparation Note
Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 µl dATP, (vial 2)
1 µl dGTP, (vial 4)
1 µl dTTP, (vial 5)
to a reaction vial.
Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):

After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS07
signalword	Warning
hcodes	H315 - H319
pcodes	P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable

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DIG-High Prime DNA Labeling and Detection Starter Kit I 11745832910

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DIG-High Prime DNA Labeling and Detection Starter Kit I has been used in a variety of hybridization techniques:

in Southern blots
in northern blots
in dot blots
in colony and plaque hybridizations
for all types of filter hybridization
for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley and wheat

DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
Features and Benefits
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 principles of greener chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG system, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Packaging
1 kit containing 7 components.
Quality
Function tested in a dot blot.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 12 labeling reactions, sufficient for 24 blots
Quality Level	100,
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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DIG-High Prime DNA Labeling and Detection Starter Kit II 11585614910

DIG-High Prime DNA Labeling and Detection Starter Kit II is a convenient kit for random-primed labeling of DNA templates with digoxigenin (DIG)-11- deoxyuridine triphosphate (dUTP), alkali-labile and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DIG-High Prime DNA Labeling and Detection Starter Kit II has been used in a variety of hybridization techniques:

in Southern blots
in northern blots
in dot blots
in colony and plaque hybridizations
for all types of filter hybridization
for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

Packaging
1 kit containing 7 components.
Principle
The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 12 labeling reactions (10 ng to 3 µg per assay), sufficient for 24 blots (blots of 100 cm²)
Торговая марка	Roche
greener alternative product characteristics	Designing Safer Chemicals Learn more about the Principles of Green Chemistry,.
shipped in	dry ice
storage temp.	20°C

pictograms	GHS05
signalword	Danger
hcodes	H315 - H318
pcodes	P264 - P280 - P302 + P352 - P305 + P351 + P338 + P310 - P332 + P313 - P362 + P364
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Digoxigenin-11-ddUTP 11363905910

Digoxigenin-11-ddUTP is a 1 mM solution of the tetralithium salt. Digoxigenin-11-ddUTP is a component of probe used for end labeling. Digoxigenin-11-ddUTP is an alternative to classical radioactive labeling for electrophoretic mobility shift assay and is highly stable compared to radioactive probes.

Digoxigenin-11-ddUTP has been used:

for labeling of DNA fragments in linker histone binding assay
for end labeling of NF-B (nuclear factor kappa-light-chain-enhancer of activated B cells) for electrophoretic motility shift assay and labelling of ATP citrate lyase gene for gel shift assay

for end labeling of DNA for fragmentation reactions
Quality
Typical analysis: 85% DIG-11-ddUTP (HPLC, area%)
Function tetsted by end labeling.

Formula: C43H61N4O20P3Li4

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	85% (HPLC)
form	solution
mol wt	(Molecular weight: M_r = 1074.7 (DIG-11-ddUTP-Li₄))
packaging	pkg of 25 µL (25 nmol; 1mM)
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

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Digoxigenin-11-dUTP, alkali-stable

Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.
Features and Benefits
Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage.
Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.
Quality
Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%).
Function tested by RPL.
Preparation Note
Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 µl nucleotide mix add 10 µl of dATP, dCTP, dGTP, dTTP (each) to 60 µl Water, PCR Grade).
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	85% (HPLC)
form	solution
mol wt	M_r 1090.7
packaging	pkg of 125 µL (11558706910 [1 mM]), pkg of 5 x 125 µL (11570013910 [5x1 mM]), pkg of 25 µL (11093088910 [1 mM])
Торговая марка	Roche
_max	222 and 292 nm, 22430 and 8780
shipped in	dry ice
storage temp.	20°C

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH2-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

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Digoxigenin-3-O-methylcarbonyl--aminocaproic acid-N-hydroxysuccinimide ester

Empirical Formula (Hill Notation):	C₃₅H₅₀N₂O₁₀
CAS Number:	129273-26-3
Molecular Weight:	658.78
MDL number:	MFCD02093394
PubChem Substance ID:	329749387

Digoxigenin-3-O-methylcarbonyl--aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).
Biochem/physiol Actions
Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.
Principle
The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.
Preparation Note
Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- -aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 µl DMSO or ethanol.

For life science research only. Not for use in diagnostic procedures.

assay	>90% (elementary analysis)
Quality Level	100,
form	powder
mol wt	M_r 658.8
packaging	pkg of 5 mg
Торговая марка	Roche
pH range	2 - 13
solubility	DMF: soluble, DMSO: soluble, ethanol: soluble
shipped in	dry ice
storage temp.	20°C
SMILES string	[H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
InChI	1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
InChI key	KHNDABJZSPPYLE-FUGFVFQCSA-N

pictograms	GHS06,GHS08,GHS09
signalword	Danger
hcodes	H300 - H319 - H331 - H373 - H410
pcodes	P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
RIDADR	UN 2811 6.1 / PGI
Flash Point F	Not applicable
Flash Point C	Not applicable

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Dispase® I (neutral protease, grade I) 4942086001

3.4.24.4 ( BRENDA | IUBMB )

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.
Specificity
Dispase® is a non-specific metalloprotease.

Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
Features and Benefits

Rapid, effective, yet gentle agent that liberates intact cells
Maintains cell membrane integrity
Non-mammalian source - free of mycoplasma and animal virus contamination
Extremely stable to influences of temperature, pH, and interference by serum components
Easily inactivated by chelating agents or by dilution
Delivers higher activity and convenience

Contents
10 vials with approximately 2mg each (= 20U/vial).
Specifications
Enzyme activity: =10U/mg (+37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Unit Definition
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides from casein corresponding to 1 µmol (181 µg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of RAS units of Dispase® with those cited in the Japanese literature (where concentrations of 1,000 to 2,000 Dispase® units /ml are not uncommon) suggests one Roche Applied Science unit of Dispase® I equals approximately 416 Japanese units of Dispase®.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 µg tyrosine per min and ml at pH 7.5 and +37 °C.
Physical form
white lyophilizate, before lyophilization filtered through 0.2 μm pore size membrane
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® I enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 µm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months, the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing.
The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

Dispase is a registered trademark of Godo Shusei Co., Ltd.

Quality Level	100,
form	lyophilized
specific activity	10 units/mg protein (20 U/vial (37 °C, casein as substrate, pH 7.5))
packaging	pkg of 10 x ~2 mg
Торговая марка	Roche
optimum pH	6.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Dispase® II (neutral protease, grade II) 4942078001

3.4.24.4 ( BRENDA | IUBMB )

Unit Definition
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol (181 µg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 µg tyrosine per min and ml at pH 7.5 and +37 °C.
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 µm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
Maintains cell membrane integrity
Non-mammalian source - free of mycoplasma and animal virus contamination
Extremely stable to influences of temperature, pH, and interference by serum components
Easily inactivated by chelating agents or by dilution
Delivers higher activity and convenience

For life science research only. Not for use in diagnostic procedures.

Dispase is a registered trademark of Godo Shusei Co., Ltd.

sterility	non-sterile
Quality Level	100,
form	lyophilized
specific activity	0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)
packaging	pkg of 5 x 1 g
Торговая марка	Roche
optimum pH	6.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNA Fragmentation Imaging Kit 6432344001

The DNA Fragmentation Imaging Kit is a simple and rapid TUNEL assay for detecting apoptosis induction in mammalian cells. DNA fragmentation, detected using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP, is measured by fluorescence microscopy or an automated imaging platform, such as the Cellavista System. The kit uses a dye to stain the nuclei of living and dead cells for quantifying total and apoptotic cell numbers, and calculating the percentage of apoptotic cells.

Programmed cell death (apoptosis) is a key pathway in mammalian cells during tissue homeostasis. Cell death malfunction has been implicated in pathology. Quantification of caspase-triggered DNA fragmentation is one of the principal techniques used to detect apoptosis induction. The DNA Fragmentation Imaging Kit performs the TUNEL assay for accurate and fast quantitative fluorescence detection using terminal deoxynucleotidyl transferase and fluorescein-labeled dUTP. The kit is ideal for measuring apoptosis in medium to high throughput cellular workflows.

Quickly determine apoptosis induction for cell-based workflows in life science research.
Reliably perform screening assays in product development, e.g., cancer research, pre-clinical safety.
Accurately measure malignant cell sensitivity in cancer research.

Packaging
1 kit containing 3 components
Quality
The kit is function tested using a cellular model (HeLa cells treated with antimycin A).
Principle
Labeling of apoptotic cells
Genomic DNA cleavage during apoptosis produces double-stranded, low molecular weight DNA fragments (mono- and oligonucleosomes), as well as single strand breaks (nicks) in high molecular weight DNA. These DNA strand breaks are identified by labeling free 3-OH termini with modified nucleotides in an enzymatic assay known as the TUNEL reaction. The TUNEL reaction preferentially labels DNA strand breaks generated during apoptosis. TUNEL assays discriminate apoptosis from necrosis, and from primary DNA strand breaks induced by cytostatic drugs or irradiation. The DNA Fragmentation Imaging Kit labels DNA strand breaks using terminal deoxynucleotidyl transferase (TdT). TdT catalyzes polymerization of labeled nucleotides to free 3-OH DNA ends in a template-independent manner using the TUNEL reaction. Fluorescein incorporated in nucleotide polymers is detected and quantified using fluorescence microscopy.
Staining of nuclei
Hoechst 33342 is a cell-permeable stain that binds DNA. It is used to stain nuclei of living or dead cells.
Preparation Note
Storage conditions (working solution): The Reaction Solution created by mixing the Enzyme Solution and Label Solution (Vial 2 and Vial 3), in Step 6 of the experiment protocol, cannot be stored. The Reaction Solution should be prepared just before use. Discard remaining unused Reaction Solution.
Storage and Stability
Store at 15 to 25 °C. (Protect from exposure to light. Store dry in dark.)

Optimized for data evaluation with Roches Cellavista System. Obtain reproducible results using automated imaging with this system.
Saves time and resources: Total assay time, including analysis, for a 96-well microplate is 85 minutes.
Reduced risk of losing cells with fewer washing steps in an improved protocol.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice

pictograms	GHS08,GHS09
signalword	Danger
hcodes	H341 - H350i - H411
pcodes	P201 - P273 - P280 - P308 + P313 - P391 - P501
RIDADR	UN 1556 6.1 / PGIII
WGK Germany	WGK 3
Flash Point F	does not flash
Flash Point C	does not flash

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DNA Isolation Kit for Cells and Tissues 11814770001

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

DNA Isolation Kit for Cells and Tissues has been used to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:

Genomic Southern blotting
Sequencing
Restriction digestion
PCR/long PCR
Cloning

Features and Benefits
Obtain increased yields of DNA in less than 2.5 hours.
Benefit from a simple, straightforward procedure (compared with column based methods).
Increase safety and convenience.
Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
Reduce purification time.
All required reagents are included in the kit.
Components

Cellular Lysis Buffer
Proteinase K Solution
RNase Solution
Protein Precipitation Solution

Quality
Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.
Preparation Note
Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.

Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues. Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse 2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse -2 collagen gene fragments (5.6 kb and 10.4 kb) and human -globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II
For life science research only. Not for use in diagnostic procedures.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 10 isolations

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNA Isolation Kit for Mammalian Blood 11667327001

Isolation of DNA from whole blood can be difficult because blood is a complex mixture containing cells, proteins, metabolites, etc. Most of the cells (>99%) are erythrocytes (red blood cells) which lack nuclei, and therefore, possess no DNA. Leukocytes (white blood cells) contain nuclei and DNA. Therefore, DNA from blood must be isolated from one of three types of leukocytes: monocytes, lymphocytes, or granulocytes.
Sample material:
Human whole blood (treated with sodium heparin, EDTA or sodium citrate): 1 to 10 mL
Isolated lymphocytes: 1 to 10 mL
Isolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes.
Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9
The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples.

DNA Isolation Kit for Mammalian Blood has been used in isolation of genomic DNA from blood of asthmatic patients for single nucleotide polymorphisms genotyping.
The DNA Isolation Kit for Mammalian Blood purifies DNA that is suitable for most molecular biology applications:

PCR/long PCR
Sequencing
Restriction digestion
Southern blotting
Cloning

DNA is easily purified in <90 minutes (plus 30 to 60 minutes resuspension time).

Process multiple samples simultaneously.

Use a cost-effective kit.

Achieve faster DNA purification for the cost of most homebrew methods.

Obtain consistent and reliable results.

Analyze different sample volumes (1 to 10 mL) with varying amounts of leukocytes.

Improve safety.

Eliminate the use of chaotropic salts, hazardous organic solvents, and column purification steps.
Components

Red Blood Cell Lysis Buffer
White Blood Cell Lysis Buffer
Protein Precipitation Solution

Quality
Each lot of kits is function tested for the ability to purify DNA from human whole blood, followed by specific amplification of a 4.8 kb tPA fragment via PCR using the Expand Long Template PCR System. The 4.8 kb tPA product is visualized by electrophoresis on an agarose gel and compared to appropriate positive and negative controls. A single 4.8 kb tPA band of equivalent intensity to the positive control is visible. The kit buffers are also tested for the absence of DNase contamination. Each solution is incubated with 1 μg pBR322 DNA for 6 hours at +37°C. The DNA is then visualized by electrophoresis on an agarose gel, and then compared to a positive control to determine if any linear or nicked plasmid DNA is visible.
Preparation Note
The DNA Isolation Kit for Mammalian Blood procedure relies on the separation of white blood cells from whole blood via a preferential red blood cell lysis. In the presence of a strong anionic detergent, the white blood cells are lysed, and then the proteins are removed by dehydration and precipitation. The purified DNA is subsequently recovered via ethanol precipitation.

Quality Level	100,
Торговая марка	Roche
packaging	kit of for 25 reactions

pictograms	GHS07
signalword	Warning
hcodes	H319
pcodes	P264 - P280 - P305 + P351 + P338 - P337 + P313
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNA, lambda 10745782001

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5 ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.

DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Quality
The DNA shows the typical cleavage pattern for wild type DNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequence
Chain Length 48,502 bp
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Storage and Stability
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
mol wt	32,300 kDa
packaging	pkg of 1 mL
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

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11467140001

DNA, MB-grade 11467140001

General description
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Sequence
Chain Length 120 to 3,000 nucleotides
Physical form
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Preparation Note
Working concentration: 100µg/ml
This fish sperm DNA should be used at a concentration of 100 µg/ml (1 : 100 dilution) in prehybridization solutions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Параметры

Quality Level 100,

form solution (ready-to-use)

mol wt 40-1,000 kDa

packaging pkg of 500 mg

Торговая марка Roche

shipped in dry ice

storage temp. −20°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany nwg

Flash Point F No data available

Flash Point C No data available

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DNase I 10104159001

Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.

Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-

The isolation of cells from lung, skin and tumor samples
Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †

Unit Definition

One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.

Preparation Note

Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	lyophilized
packaging	pkg of 100 mg
Торговая марка	Roche
optimum pH	~7.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I 11284932001

3.1.21.1 ( BRENDA | IUBMB )

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
form	lyophilized
packaging	pkg of 100 mg
Торговая марка	Roche
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I recombinant 4536282001

3.1.21.1 ( BRENDA | IUBMB )

Double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Properties: Double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Activity: 10,000U/bottle according to Kunitz (+25°C; DNA as substrate).
Unit definition: One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minutes under assay conditions.

Features and Benefits
Contents
Lyophilizate
Unit Definition
One unit, according to Kunitz, is the enzyme activity that under assay conditions causes an absorbance increase at 260 nm of 0.001/minute in 1 ml. Volume activity is determined in 0.1 M sodium acetate, 5 mM MgSO4, pH 5.0.
Assay mixture: Calf thymus DNA (100 µg) is incubated with 20 to 50 U DNase I at +25 °C.
The increase in absorbance is measured at 260 nm.
Information Note: These conditions are used for the determination of activity,according to Kunitz, since the Kunitz assay results in high reproducibility and sensitivity. When using this enzyme for normal experimental purposes, Roche recommends using incubation buffers that are appropriate for a given application, e.g., as mentioned in the standard literature (Sambrook et al., Curr. Prot. Mol. Biol., etc.).
Preparation Note
Activator: The enzyme requires divalent cations for maximal activity. The specificity of the reaction depends on the nature of the cation used. In the presence of Mg2+ DNase I causes single-stranded nicks in dsDNA, while in the presence of Mn2+ the enzyme produces double-stranded breaks.
Storage conditions (working solution): Long-term Storage of the Dissolved Enzyme
The solvent generally recommended for DNase I is water. When DNase I is reconstituted in water, the solution can be kept for 2 days at 2 to 8 °C and for 1 month at -15 to -20 °C. For best results, prepare appropriate aliquots and avoid repeated freezing and thawing. However, for long-term storage, carefully dissolve DNase I in one of the buffers listed below. In buffer 1, the enzyme will be stable for several weeks. The enzyme will be stable for at least 18 months if it is dissolved in buffer 2, 3, or 4 and stored at the recommended temperatures.
Information note: Do not vortex the enzyme while dissolving!
Buffer 1: 20 mM Tris-HCl, 20 mM MgCl2, 5 mM CaCl2, 0.1 mM dithioerythritol, 0.1 mM EDTA, 50% (v/v) glycerol, pH 8. Store this solution at -15 to-25 °C; it will not freeze at this temperature and will be stable for several weeks.
Buffer 2: 20 mM Tris-HCl, 50 mM NaCl, 1 mM dithioerythritol, 100 µg/mL BSA, 50% glycerol (v/v), pH 7.6. Store this solution at -15 to -25 °C for up to 18 months; it will not freeze at this temperature.
Buffer 3: 20 mM Tris-HCl, 1 mM MgCl2, 50% (w/v) glycerol, pH 7.5. Store this solution at -15 to -25 °C for up to 18 months; it will not freeze at this temperature.
Buffer 4: 20 mM Tris-HCl, 1 mM MgCl2, pH 7.5. Aliquot in appropriate amounts (e.g., 10 µl), freeze the aliquots quickly on dry ice, and store at -60 °C or below for up to 18 months. Thaw only the amount needed for each experiment. Do not refreeze. Discard any leftover thawed solution.

DNase I, recombinant, grade I is used for:Eliminating DNA during protein isolation procedures
Analysis of chromatin structure
Eliminating DNA during sample preparation

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 2 x 10,000 U
Торговая марка	Roche
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS08
signalword	Danger
hcodes	H317 - H334
pcodes	P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DNase I recombinant, RNase-free 4716728001

3.1.21.1 ( BRENDA | IUBMB )

Recombinant DNase I is a DNA-specific endonuclease.The enzyme catalyzes the degradation of both double- and single-stranded DNA randomly by hydrolyzing phosphodiester linkages to DNA, resulting in a mixture of oligo- and mononucleotides. All material used during the production process of DNase I recombinant is non-animal sourced, resulting in an animal-free product.
Contents

Recombinant DNase I, RNase-free, 10 U/µl
Incubation Buffer, 10x concentrated

Specificity

Heat inactivation: One unit DNase I recombinant, RNase-free is heat-inactivated by 10 minutes incubation at 75 °C.
Important Note: Alternatively, DNase I recombinant, RNase-free can be inactivated and removed by phenol extraction according to standard protocols, e.g., Current Protocols in Molecular Biology.

DNase I recombinant, RNase-free may be used to degrade DNA in applications that are sensitive to the presence of RNase. For example, DNase I is frequently used to:

Remove genomic DNA from RNA preparations prior to RT-PCR
Isolate DNA-free RNA after in vitro transcription reactions
Perform nick translations
Map DNase-sensitive regions in eukaryotic DNA

1 kit containing 2 components

Eliminates DNA contamination from any RNA sample
Contains no detectable RNase or protease activity
Can be heat inactivated, thereby eliminating the need for organic extraction
Shipped with an optimized incubation buffer, which supports maximum DNase activity
Produced via an entirely animal-free process, to eliminate any risks associated with animal-derived material

Packaging

Quality

Absence of contaminants: Each lot is tested to ensure the absence of RNases and proteases according to the current Quality Control procedures.

Specifications

Glycosylated form
Recombinant DNase I is heterogeneously N-glycosylated, so it appears as two bands in gel electrophoresis.
Divalent ion requirement
DNase I requires divalent cations for maximum activity. The DNA-specific endonuclease is activated by ions such as magnesium ions and is stimulated by calcium ions. Therefore, the enzyme is inhibited by metal chelating agents like EDTA.

Unit Definition

One unit is the enzyme activity that effects an absorbance increase of 0.001/minute under assay conditions in 1 ml at 260 nm.
Assay conditions:
Volume activity is determined according to the following assay mixture. 100 µg calf thymus DNA is incubated in 2.5 ml 1x incubation buffer with 40 to 70 units DNase I recombinant, RNase-free at +25 °C. The absorbance increase is measured at 260 nm.

Preparation Note

Activator: Bivalent metal ions
Working solution: Storage Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2, 2 mM MgCl2, 1 mM dithioerythritol, 0.1 mg/ml Pefabloc SC, 50% glycerol (v/v), pH 7.6 (at 4 °C).
Incubation Buffer (10x): 400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Enzyme Dilution Buffer: 25 mM Tris-HCl, 50% glycerol (v/v), pH 7.6 (at 4 °C).

Storage and Stability

Store undiluted enzyme solution at -15 to -25°C; storage buffer at 4 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	bovine pancreas
recombinant	expressed in Pichia pastoris
form	solution
mol wt	~39 kDa
packaging	pkg of 10,000 U
Торговая марка	Roche
optimum pH	7.0-8.0
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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DOTAP Liposomal Transfection Reagent 11202375001

Empirical Formula (Hill Notation):	C₄₃H₈₃NO₈S
CAS Number:	144189-73-1
Molecular Weight:	774.19
MDL number:	MFCD16660441
PubChem Substance ID:	329749323

DOTAP Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. Unlike other liposomal reagents, it produces highly efficient transfections both in the presence and absence of serum. It can also be successfully used for in vivo applications. Mixing DOTAP with DNA results in spontaneously formed stable complexes. These complexes can be added directly to the cell culture medium. This method of DNA transfer is very gentle, avoiding the cytotoxic effects commonly encountered with lipofection or other transfection methods.

The DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate) Liposomal Transfection Reagent is a liposome formulation of the cationic lipid DOTAP. DOTAP can be used for the highly efficient transfection of DNA including yeast artificial chromosomes (YACs) into eukaryotic cells for transient or stable gene expression, and is also suitable for the efficient transfer of other negatively charged molecules, such as RNA, oligonucleotides, nucleotides, ribonucleo-protein (RNP) complexes, and proteins into research samples of mammalian cells.
Cationic liposome-forming compound for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells. Mixing DOTAP with DNA results in spontaneously formed stable complexes that can be directly added to cell culture medium. These complexes fuse with the cell membrane and release DNA into the cytoplasm. The cells are transfected efficiently without cytotoxic effects. Use approximately 5-10 μg DOTAP per μg DNA and approximately 10-20 μg DOTAP per mL cell culture medium. The optimal working concentration and transfection time depends in part on the cell line used, and the type of material (RNA, DNA, protein) loaded.
Features and Benefits

Easy to use: The lipid dispersion is simply mixed with the DNA solution, then applied directly to the cells
Highly effective: 5 to 100-fold more effective than the calcium phosphate or DEAE-dextran method
Gentle: No cytotoxic effects
Flexible: Equally effective in the presence or absence of serum. Effective on a wide range of species (e.g., insect cells, mammalian cells) and a variety of different cell types. Can be used for either transient or stable transfection procedures.
Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered.

Preparation Note
Working concentration: Approximately 5 - 10µg DOTAP/µg DNA, and 10 - 20µg DOTAP/ml cell culture medium.
The optimal working concentration is dependent on several parameters, including the cell line being used, concentration and type of nucleic acid (DNA, RNA), and incubation time. It is important to optimize the transfection conditions for the individual cell type studied.
Cytotoxicity: Not cytotoxic up to a concentration of 100µg/ml (PBLs, HeLa cells).

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>99% (TLC)
packaging	pkg of 5 x 400 μL (10 U/μl)
Торговая марка	Roche
application(s)	transfection: suitable
shipped in	wet ice
storage temp.	2-8°C
SMILES string	[H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.O=S([O-])(OC)=O
InChI	1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-;
InChI key	RSMRWWHFJMENJH-LQDDAWAPSA-M

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1µg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10µl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 x Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.
Dpn I requires methylation of adenine residues for activity and thus digests only GmATC sequences containing N6-methyladenine. Such methylated sequences are produced by dam methylase. This requirement for methylation distinguishes Dpn I from Mbo I, which is inhibited by dam methylation, and Sau3A I, which is not influenced by dam methylation. Dpn I also cleaves at a different position on the recognition sequence than Mbo I and Sau3A I.
Specificity
Recognition sites: GmAT*C
GmAT*C
Restriction site: GmAT*C
GmAT*C
Heat inactivation: No inactivation of Dpn I after incubation at 65 °C for 15 minutes.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	does not flash
Flash Point C	does not flash

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Dpn I

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.
Quality
Absence of nonspecific endonuclease activities
1µg pBR322 DNA is incubated for 16 hours in 50µl SuRE/Cut Buffer A with an excess of Dpn I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5µg [3H] labeled calf thymus DNA are incubated with 3µl Dpn I for 4 hours at +37°C in a total volume of 100µl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
Compatibility
Dpn I generates fragments with blunt ends that are compatible with any blunt end.
DNA Profile
Number of cleavage sites on different DNAs

: 116
X174: 0
Ad2: 87
M13mp7: 8
pBR322: 22
pBR328: 27
pUC18: 15
SV40: 8

Unit Definition
One unit is the enzyme activity that cleaves 1 µg pBR322 DNA in one hour at +37 °C in a total volume of 25 µl (1x) SuRE/Cut Buffer A. Since complete Dpn I digestion of pBR322 DNA needs fully methylated GATC sequences < 5% partial digested bands may be observed during activity determination.
Analysis Note
Activity in PCR buffer: 100%
Relative activity in PCR mix (Taq DNA Polymerase buffer) is 100%. The PCR mix contained DNA, primers, 10 mM Tris-HCl (pH 8.3, 20 °C), 50 mM KCl, 1.5 mM MgCl2, 200 µM dNTPs, 2.5 U Taq DNA polymerase. The mix was subjected to 25 amplification cycles.Activity in reaction buffer of Pwo SuperYield DNA Polymerase PCR Mix is 100%; Pwo SuperYield DNA Polymerase PCR Mix is not available in U.S.
SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

A: 100%
B: 75-100%
H: 75-100%
L: 50-75%
M: 75-100%

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	solution
packaging	pkg of 1,000 U (10742988001 [10 U/µl]), pkg of 200 U (10742970001 [10 U/µl])
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

Specificity
The thiol protease inhibitor E-64 specifically inhibits papain and other cysteine proteases such as cathepsin B and L, bromelain, and ficin. Cathepsin A and D are not inhibited. L-lactate dehydrogenase from porcine heart is not inhibited, although the enzyme contains a functional thiol group.

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E-64

Empirical Formula (Hill Notation):	C₁₅H₂₇N₅O₅
CAS Number:	66701-25-5
Molecular Weight:	357.41
Beilstein/REAXYS Number:	1405664
MDL number:	MFCD00080261
PubChem Substance ID:	329798998

Кат. номер

11585681001

10874523001

E-64 is used for the inhibition of papain and other cysteine proteases such as cathepsin B and L, bromelain, and ficin during isolation and purification of proteins and enzymes.
It has been used in the affinity chromatography for the purification of hemoglobin receptors.
The inhibition of thiol proteases by E-64 appears to be of a non-competitive nature between the SH components. The inhibition is also irreversible, and is altered after gel filtration (Sephadex column) or dialysis after incubation of papain with E-64. The enzyme and inhibitor combine in an equimolar ratio.
E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.
Biochem/physiol Actions
E-64 is an irreversible, potent, and highly selective cysteine protease inhibitor. E-64 does not react with the functional thiol group of non-protease enzymes, such as L-lactate dehydrogenase or creatine kinase. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 is a very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition, it is permeable in cells and tissues and has low toxicity.
Preparation Note
Working concentration: 0.5 to 1.0 µg/ml (1.4 to 28.0 µM)
Working solution: Soluble to 20 mg/ml (stock solution) in a 1:1 (v/v) mixture of ethanol and water (vortexing or slight warming in water bath (40 °C) may facilitate dissolution). Also soluble in a neutral water/methanol solution, in water, methanol, acetic acid, pyridine and DMSO. Sparingly soluble in ethanol and propanol. Insoluble in acetone, chloroform, ethyl ether and benzene.
Storage conditions (working solution): -15 to -25 °C
Solutions are stable for one month when stored in aliquots at -15 to -25 °C.
E-64 is soluble in water. A 20 mg/ml solution can be prepared in water (heat may be needed). A suggested stock solution is a 1 mM aqueous solution. The effective concentration for use as a protease inhibitor is 1 to10 μM. Aqueous stock solutions are stable for months at -20 °C. Diluted solutions are stable for days at neutral pH. E-64 is stable from pH 2-10, but is unstable in ammonia or in HCl. E-64 is also soluble in DMSO, a 10 mM solution can be prepared in dry DMSO and stored at -20 °C. Subsequent dilutions were in culture medium. Solutions for injection were prepared by dissolving E-64 in 0.9% sodium chloride or in a minimum amount of saturated sodium bicarbonate followed by dilution with 0.9% sodium chloride (after adjusting the pH to 7.0 with acetic acid).
Reconstitution
Soluble to 20 mg/ml in a 1:1 (v/v) mixture of ethanol and water. Solutions are stable for one month when stored in aliquots at -15 to -25 °C.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
assay	>95% (HPLC)
mol wt	M_r 357.4
packaging	pkg of 10 mg (10874523001), pkg of 25 mg (11585681001)
Торговая марка	Roche
pH range	2 - 10
solubility	ethanol: water (1:1): soluble 20 mg/mL
shipped in	wet ice
storage temp.	2-8°C
SMILES string	CC(C)C[C@H](NC(=O)[C@@H]1O[C@H]1C(O)=O)C(=O)NCCCCNC(N)=N
InChI	1S/C15H27N5O5/c1-8(2)7-9(20-13(22)10-11(25-10)14(23)24)12(21)18-5-3-4-6-19-15(16)17/h8-11H,3-7H2,1-2H3,(H,18,21)(H,20,22)(H,23,24)(H4,16,17,19)/t9-,10+,11+/m0/s1
InChI key	LTLYEAJONXGNFG-HBNTYKKESA-N

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Endoproteinase Arg-C Sequencing Grade 11370529001

3.4.22.8 ( BRENDA | IUBMB )

Endoproteinase Arg-C Sequencing Grade is isolated from Clostridium histolyticum as a highly purified and specific cysteine protease.
Specificity
Endoproteinase Arg-C Sequencing Grade is a cysteine protease that specifically cleaves peptide, ester, and amide bonds at the C-terminal side of arginine residues. The specificity is primarily to arginine residues, although hydrolysis proceeds to a minor degree in most lysine-containing substrates. Reducing agents (such as DTT) and Ca2+ are required for full activity.
The specificity of Endoproteinase Arg-C Sequencing Grade is tested with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Endoproteinase Arg-C Sequencing Grade are incubated for prolonged incubation times to detect traces of impurities such as contaminating proteases.
Recognition sites: C-terminal side of arginine residues, other recognition sites with lower efficiency.

Use Endoproteinase Arg-C Sequencing Grade for the specific cleavage of proteins and peptides for peptide mapping, fingerprinting, and sequence analysis. The protease is suitable to digest proteins in solution, in gels, or on blotting membranes.
Quality
Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.
Preparation Note
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and -mercaptoethanol.
Inhibitors: Oxidizing agents, sulfhydryl reactants (e.g., TLCK), EDTA, Co2+, Cu2+, Cd2+. Citrate, borate, and Tris anions partially inhibit.
Optimum pH: 7.2 - 8.0
Storage conditions (working solution): -15 to -25°C
For long-term use, the solution should be gently flushed with a stream of nitrogen and stored at -15 to -25°C. The reconstituted incubation buffer is stable for several months at -15 to -25°C.
Reconstitution
The lyophilized endoproteinase Arg-C sequencing grade is reconstituted in 50 µl double-dist. water to give a final concentration of 50 mM Tris-HCl buffer, 10 mM CaCl2, 5 mM EDTA, pH 8.0. The lyophilized activation solution is dissolved in 100 µl double-dist. water resulting in a final 50 mM dithiothreitol-(DTT-)concentration and 5 mM EDTA-concentration.

For life science research only. Not for use in diagnostic procedures.

form	lyophilized
mol wt	M_r 59 kDa
packaging	pkg of 3 x 5 µg
Торговая марка	Roche
parameter	37 °C optimum reaction temp.
optimum pH	7.2-8.0
shipped in	wet ice
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS08
signalword	Danger
hcodes	H302 - H315 - H318 - H334
pcodes	P261 - P264 - P280 - P304 + P340 - P305 + P351 + P338 + P310 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Endoproteinase Glu-C (V8 Protease) 10791156001

3.4.21.19 ( BRENDA | IUBMB )

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.
Specificity
Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.
Biochem/physiol Actions
Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
Preparation Note
Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Storage and Stability
Store at 2 to 8 °C. (Store dry!)

For life science research only. Not for use in diagnostic procedures.

form	lyophilized (salt-free)
specific activity	20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).), ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)
mol wt	30 kDa
packaging	pkg of 2 mg
Торговая марка	Roche
optimum pH	8.0-8.5
shipped in	wet ice

pictograms	GHS07,GHS08
signalword	Danger
hcodes	H315 - H319 - H334 - H335
pcodes	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
Target Organs	Respiratory system
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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Enterokinase

3.4.21.9 ( BRENDA | IUBMB )

Enterokinase is a protease from calf intestine and supplied in a quality optimized for the cleavage of fusion proteins.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Specificity
Serine protease acting as a restriction protease that recognizes the amino acid sequence -(Asp)4-Lys-X. The aspartic acid residues can be partially substituted by glutamic acid.

Quality
Purity: highly purified, not standardized with albumin
Quality control: function tested
Enterokinase is used for the cleavage of fusion proteins at definite cleavage sites. For the processing of recombinant proteins, the desired protein is fused with Enterokinase recognition sequence. After purification of the entire fusion protein, the protein or peptide is released by incubation with enterokinase.
Preparation Note
Working concentration: 1:50 (w/w)
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.

For life science research only. Not for use in diagnostic procedures.

form	lyophilized
mol wt	150 kDa
packaging	pkg of 3 x 250 µg (11351311001), pkg of 3 x 30 µg (11334115001)
Торговая марка	Roche
concentration	1:50 % (w/w)
optimum pH	8
shipped in	wet ice
storage temp.	2-8°C

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Epidermal Growth Factor, human (hEGF) 11376454001

Recombinant Epidermal Growth Factor, human (hEGF), is produced in E. coli and purified by standard chromatographic techniques. It is a 100µg white lyophilizate, filtered through 0.2µm pore size membrane, recombinant from E. coli.
Epidermal growth factor (EGF) is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids EGF is produced in the tubular cells of the submaxillary gland of the mouse, in the acinar cells of the human submaxillary gland, and in the human duodenal glands.
Human EGF is identical to -urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion. 37 amino acids of the 53 amino acids comprising the longer urogastrone (human EGF) and mouse EGF are common to both peptides (70% homology) and the three disulfide bonds are formed in the same relative positions. The biological activity of human EGF is similar to that of mouse EGF. The cellular receptor for EGF is the best understood growth factor receptor. The oncogene v-erbB codes for a product homologous to a portion of the EGF receptor in which the EGF-binding domain has been deleted. Evidence exists suggesting that this truncation of the EGF receptor may lead to constitutive activation without requirement for ligand binding.
EGF stimulates the proliferation and differentiation of awide variety of cells of ectodermal and mesodermal origin (e.g., fibroblasts, glial cells, keratinocytes, epithelial cells, endothelial cells, chondrocytes). EGF is a constituent of many serum-free media formulations for a variety of cells.
Specificity
Species Specificity: Human EGF is effective on human and mouse cells.
Specific activity: <0.5 ng/ml, at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Epidermal growth factor (EGF) stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, and is a constituent of many serum-free media formulations. It has been used in the cell differentiation assay.
Sequence
The primary structure of recombinant, human EGF (one polypeptide chain, 54 amino acids) is identical to that of human, natural EGF (β-urogastrone) (53 amino acids), however, recombinant EGF has an additional methionine at the amino-terminus.
Chain Length 54 AA
Unit Definition
Unit Conversion: 1 BM unit = 3.25 NBSB units (natural IL-2)1 BM unit = 3.25 NBSB units (natural IL-2)
Unit Definition: EC50 defintion: The concentration of hEGF that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with MK cells.
Physical form
White lyophilizate, filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: 0.5 to 20 ng/ml
Human EGF exerts its biological activity in the concentration range of 0.5 to 20 ng/ml. Recommended concentration for serum-free cell culture is 1 to 10 ng/ml
Working solution: In sterile water (final concentration: 500 µg/ml), further dilution with medium or PBS (phosphate buffered saline) containing 1 mg/ml BSA (bovine serum albumin), or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the reconstituted solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in E. coli
assay	>95% (HPLC)
form	lyophilized
potency	<0.5 ng/mL EC₅₀
mol wt	6200 Da
packaging	pkg of 100 µg
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
color	white
UniProt accession no.	Q6QBS2,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... EGF(1950)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

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EPO ELISA 11693417001

Erythropoietin (Epo) is a cytokine belonging to the hematopoietic cytokine superfamily. It has structural similarity to growth hormone and is encoded as a 193 amino acid polypeptide comprising of canonical leader sequence composed of 27 amino acid, and a carboxyl terminal arginine which is removed by posttranslational cleavage. Furthermore, Epo is produced in adult kidney and fetal liver and is involved in stimulating red blood cell production.
Specificity
The ELISA system detects both natural and recombinant human EPO from CHO cells. No cross-reaction with other serum components has been found.

Immunogen

Recognized epitope not known.

For research use only. Not for use in diagnostic procedures.
The EPO ELISA is designed for use in life science research studies as a method for the determination of EPO concentrations in plasma and serum; it is not intended for diagnostic procedures. The literature indicates that in certain forms of anemia, specifically in aplastic renal anemia, the regulation of EPO formation in the kidney appears to be intact. This may lead to an extremely high EPO concentration in serum of the respective individuals.
Exaggerated EPO levels, accompanied by a normal or even elevated hemoglobin concentration (and/or hematocrit values), have been found in a series of diseases (e.g., tumors of the kidney or liver, or in secondary forms of polycythemia). Polycythemia vera, however, is characterized by an abnormally increased red blood cell count in tandem with a low EPO serum level. The EPO ELISA is intended to help increase scientific knowledge about these relationships.
Packaging
1 kit containing 10 components.
Unit Definition
Unit Conversion: The EPO concentration of unknown samples is calculated from a calibration curve. Each lot of standards (used for the calibration curve) is calibrated according to the 2. IRP WHO preparation (see lot-specific label). Conversion factor: 1 mlU/ml corresponds to 6.6 pg/ml. Conversion factor: 1 mU = 6.6 pg.
The normal range of a healthy human lies between 4 to 90 mIU/ml.
Preparation Note
Working solution: Solution 1 hEPO control serum (Bottle 1)
Reconstitute the lyophilizate in 500 µl double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A slightly milky solution.
Stability of Solution:

1 week at 2 to 8 °C
12 months at -15 to -25 °C

For Use in: step 1
Solution 2 Anti-hEPO-HRP (Bottle 2)
Reconstitute the lyophilizate in 500 µl double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A clear, colorless solution.
Stability of Solution:

12 months at 2 to 8 °C. Warning: do not freeze

For Use in: solution 9
Soluton 3a to 3f hEPO standard (Bottles 3a-3f)
Reconstitute the lyophilizate in 1 ml double-distilled water for 10 min at 15 to 25 °C and mix thoroughly.
Warning: DO NOT VORTEX.
Result: A clear, colorless solution.
Stability of Solution:

8 hours at 2 to 8 °C
aliquot the rest to be used for subsequent standard curves and store at -15 to -25 °C

For Use in: step 1
Solution 4 Incubation buffer (Bottle 4)

100 ml
Ready-to-use solution, clear, colorless

Stability of Solution: see kit label imprint
For Use in: solution 9
Solution 5 Sample buffer (Bottle 5)

100 ml
Ready-to-use solution, clear or turbid, yellowish

Stability of Solution: see kit label imprint
For Use in: step 1
Solution 6 Washing buffer (Bottle 6)
Dissolve one tablet in 2 l of double-distilled water.
Result: A clear, colorless solution.
Stability of Solution: 12 months at 2 to 8 °C
For Use in: step 2
Solution 7 TMB substrate solution (Bottle 7)

15 ml
Ready-to-use solution, clear colorless to slightly yellowish.

Stability of Solution: see kit label imprint
For Use in: step 3
Solution 8 TMB stop solution (Bottle 8)

7 ml
Ready-to-use solution, clear, colorless

Stability of Solution: see kit label imprint
For Use in: step 4
Solution 9 Immunoreagent
For 100 wells (5 ml): Add 250 µl reconstituted anti-hEPO-HRP (solution 2) to 4.75 ml incubation buffer (solution 4), and mix well.
Stability of Solution: 8 hours at 2 to 8 °C. Warning: prepare shortly before use
For Use in: step 1
Storage conditions (working solution): Solution 1 hEPO control serum (bottle 1)
1 week at 2 to 8 °C
12 months at -15 to -25 °C
Solution 2 Anti-hEPO-HRP (bottle 2)
12 months at 2 to 8 °C
do not freeze!
Soluton 3 hEPO standard (bottles 3a-3f)
8 hours at 2 to 8 °C
aliquot the rest to be used for subsequent standard curves and store at -15 to -25 °C
Solution 4 Incubation buffer (bottle 4)
see kit label imprint
Solution 5 Sample buffer (bottle 5)
see kit label imprint
Solution 6 Washing buffer (bottle 6)
12 months at 2 to 8 °C
Solution 7 TMB substrate solution (bottle 7)
see kit label imprint
Solution 8 TMB stop solution (bottle 8)
see kit label imprint
Solution 9 Immuno-reagent
prepare shortly before use
8 hours at 2 to 8 °C

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 96 tests
Quality Level	100,
Торговая марка	Roche
storage temp.	2-8°C

pictograms	GHS05,GHS07,GHS09
signalword	Warning
hcodes	H290 - H315 - H317 - H319 - H411
pcodes	P261 - P264 - P273 - P280 - P333 + P313 - P391
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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Erythropoietin, human (hEPO) 11120166001

Recombinant, Erythropoietin, human (hEPO), is produced in CHO cells (chinese hamster ovary) and purified by standard chromatographic techniques. The glycoprotein encoded by erythropoietin (EPO) is synthesized in kidneys. It is the principal hormone that has a short half-life. Cells responsive to EPO have been identified in adult bone marrow, fetal liver, or adult spleen.
Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specificity
Human Erythropoietin (hEPO) is effective on mouse and human cells.

Erythropoietin (EPO) is a glycoprotein which stimulates proliferation and differentiation of erythroid precursor cells (CFU-E, BFU-E) to more mature erythrocytes.
Biochem/physiol Actions
Erythropoietin (EPO) controls the synthesis of RBCs (red blood cells) by inducing the proliferation and differentiation of erythroid precursor cells. As it induces erythropoiesis, EPO can be used to treat anemia of cancer patients.
Sequence
One polypeptide chain (166 amino acids), glycosylated, identical to natural hEPO. The carbohydrate structure of recombinant EPO isolated from CHO cells is very similar to that of natural EPO.
Unit Definition
EC50 definition: The amount of hEPO that is required to produce equivalent [3H]-thymidine incorporation into spleen cells from phenylhydrazine-treated mice to that expressed by 1 unit of the WHO-EPO reference standard (2nd IRP) (1 unit equals 10 ng).
Physical form
Solution, filtered through 0.2 μm pore size membrane.
Preparation Note
Working solution: Dilute the concentrated EPO solution (250 U/ml) with PBS or culture medium containing 1 mg/ml (0.1%) BSA (or HSA) or 1 to 10% serum.
Storage conditions (working solution): -15 to -25 °C
Store the solution in aliquots at -15 to -25 °C
Note: Avoid repeated freezing and thawing

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human
recombinant	expressed in CHO cells Hamster
assay	>98% (HPLC and SDS-PAGE)
form	solution
packaging	pkg of 250 U (2.5 µg, 1 ml)
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
impurities	<1 EU/µgtested (LAL test)
UniProt accession no.	P01588,
storage temp.	20°C
Gene Information	human ... EPO(2056)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	No data available
Flash Point C	No data available

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Expand™ 20 kbPLUS PCR System, dNTPack 4743814001

The Expand 20 kbPLUS PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase, a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture and associated buffer system is designed to give a high yield of PCR product when fragments longer than 20 kb need to be amplified.
T/A cloning can also be performed.
The kit has control human genomic DNA and a set of control primers for amplifying a 23kb fragment. This test can be used to test template DNA and primer pair performance.
Use 3.75 U for a standard 50µL PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
To obtain long PCR products, first evaluate length and purity of the DNA template. Handling all genomic DNA templates with care. Genomic DNA template should be larger than 50kb, as determined with agarose gel electrophoresis.

Choose Expand™ 20 kbPLUS PCR System when amplifying genomic DNA fragments up to 35 kb. Expand 20 kbPLUS PCR System features a specifically optimized buffer and enzyme-blend mixture to amplify extra-long pieces of DNA (over 20 kb). Control primers and DNA produce fragments of 23 kb in length.

PCR
RT-PCR
Large-fragment amplification

Packaging
1 kit containing 7 components
Quality
Each lot of Expand 20kbPLUS PCR System is function-tested in PCR using human genomic DNA and specific primers for -globin to amplify a 23kb fragment.
Unit Definition
Volume Activity: 5 U/μl
Storage and Stability
Store supplied human control DNA at 2–8 °C, all other components at -15 to -25°C.

Go the limit: The buffer and enzyme-blend amplify products over 20kb DNA.
Easy PCR monitoring: Human genomic DNA and human control -globin primers amplify a 23kb fragment.
Test template quality: Control reagents also test template quality and primer pair performance.
Cost-effective: Use the convenient premixed solution of PCR grade dNTPs.

For life science research only. Not for use in diagnostic procedures.

usage	sufficient for 40 reactions
feature	Long Range PCR, dNTPs included, hotstart: no
packaging	pkg of 200 U
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
parameter	68 °C optimum reaction temp.
application(s)	PCR: suitable
input	purified DNA
shipped in	dry ice
storage temp.	20°C (15°C to 25°C)

hcodes	H412
pcodes	P273 - P501
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	does not flash
Flash Point C	does not flash

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Expand™ High Fidelity PCR System

The 5′ → 3′ polymerase activity of Taq DNA Polymerase and the 3′ → 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′ → 5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. PCR up to 9 kb is possible, with yield diminishing as DNA fragment length increases. The Expand High Fidelity PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA polymerase. T/A cloning is recommended.

The Expand™ High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length in:

PCR
RT-PCR

Use Expand High Fidelity PCR System, dNTPack with ready-to-use PCR nucleotide mix.
For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.
Features and Benefits
Expand High Fidelity PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity for high yield and fidelity. It is optimized for PCR of DNA fragments up to 5 kb.
Use 2.6 U for a standard 50 µl PCR.
Volume activity: 3.5 U/µl

Use one enzyme for all applications.

Enjoy high yield, fidelity, and flexibility.

Detect PCR products

previously undetectable and avoid false negatives.

Achieve successful results

from small quantities of template DNA.
Packaging
1 kit containing 4 components
Quality
Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1 kb amplified product) and the tPA gene (4.8 kb amplified product).
Unit Definition
Volume Activity: 3.5 U/μl

For life science research only. Not for use in diagnostic procedures.

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche

Quality Level	100,
usage	sufficient for 1,000 reactions (11759078001), sufficient for 200 reactions (11732650001), sufficient for 40 reactions (11732641001)
packaging	pkg of 2,500 U (11759078001 [10x250 U]), pkg of 100 U (11732641001), pkg of 500 U (11732650001 [2x250 U])
Торговая марка	Roche
shipped in	dry ice
storage temp.	20°C

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Expand™ Long Template PCR System

Expand Long Template PCR System is an enzyme mix that contains thermostable Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture produces a high yield of PCR product from genomic DNA. Expand Long Template PCR System allows amplification of DNA fragments up to 20kb from human genomic DNA, and 40kb from DNA.
The Expand Long Template PCR System copies DNA threefold more accurately than Taq DNA Polymerase.
Misincorporations cause Taq DNA polymerase to fall off the DNA strand. The 3 5 exonuclease activity of the proofreading polymerase reaction mix amplify DNA three times more accurately than Taq DNA polymerase alone.
Long PCR products require a special buffer and optimized protocol. This kit has three buffers for amplifying fragments from 0.5 to 9 kb, 9 to 12 kb, and larger than 12kb. For high template quality, use Roches High Pure Template Preparation Kit to prepare genomic DNA template.

The main application of the Expand Long Template PCR System is the accurate amplification of very long target sequences, even from genomic DNA. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length.
Choose 1st generation Expand Long Template PCR System for long and accurate PCR reactions. Use this optimized enzyme blend and specially developed three-buffer set to generate PCR products from 5 to 20kb in length. Use the product for:

PCR
RT-PCR or long-range PCR
Large-fragment amplification
Genome mapping and sequencing
Contig construction
Characterization of cloned sequences in lambda phages or cosmids
Rapid identification and cloning of complete genes from genomic DNA or cDNA sequence as starting material

As economical alternative we recommend to update to 2nd generation Expand Long Range, dNTPack.
For amplicon sizes of 20 to 35kb choose Expand 20kbPLUS PCR System, dNTPack.
Features and Benefits
Expand Long Template PCR System is ideal for genome mapping and sequencing, contig construction, characterization of cloned sequences in lambda phages or cosmids, and eukaryotic-gene cloning and analysis.

Amplify longer templates: This 3-buffer set amplifies products from 5 to 20kb.
Achieve higher yields and 3-fold higher fidelity compared to Taq DNA Polymerase.
Improve your PCR: Obtaining more full-length PCR product is ideal downstream.

Packaging
1 kit containing 4 components
Quality
Each lot is PCR function-tested using human genomic DNA and specific primers for a 9kb, 12kb, and 15kb fragment. The enzyme mix is tested for the absence of any contaminating activities, including endo- or exonucleases and nicking activity according to the current Quality Control procedures.
Unit Definition
Volume Activity: 5 U/μl
Preparation Note
Working concentration: Optimal enzyme concentration varies from 0.5 to 5.0 U per 50 µl reaction. The standard enzyme concentration is 3.75 U per 50 µl reaction.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
usage	sufficient for 190 reactions (11681842001), sufficient for 38 reactions (11681834001), sufficient for 950 reactions (11759060001)
packaging	pkg of 3,600 U (11759060001 [10x360 U]), pkg of 150 U (11681834001), pkg of 720 U (11681842001 [2x360 U])
Торговая марка	Roche
parameter	68 °C optimum reaction temp.
shipped in	dry ice
storage temp.	20°C

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FastStart™ Taq DNA Polymerase, 5 U/l

FastStart™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart™ Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.

FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
It can be applied for:

PCR
Multiplex PCR
Difficult templates e.g., secondary structures or GC-rich sequences
Automated PCR e.g., handling at room temperatures
Hot Start PCR up to 3kb
Hot Start RT-PCR up to 3kb
Quantitative reverse transcription PCR (RT-qPCR)
Bisulfite-specific PCR

Use FastStart™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2x concentrated ready-to-use FastStart™ PCR Master.
Features and Benefits
FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations.

Higher specificity, sensitivity, and yield:

Hot start PCR makes PCR setup easier.

Use robotic setup.

Use this enyzme mix stable for 24hours at +15 to +25°C.

Prevent PCR carryover contamination.

Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes

optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates
superior results due to its unique enzyme design and optimized buffer system

Packaging
1 kit containing 5 components
Quality
Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.
Unit Definition
1 µg M13mp9ss DNA, 0.3 µg M13 sequencing primer and 0.1 µCi -32P dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Unit Assay: 1 µg M13mp9ss DNA, 0.3 µg M13 sequencing primer and 0.1 µCi -32P dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 µl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.
Volume Activity: 5 U/µl
Preparation Note
Storage conditions (working solution): Fast Start Taq mastermix is stable for 4 weeks at -25 °C to -15 °C without primers
Please note: This parameter is not part of specification

For life science research only. Not for use in diagnostic procedures.

NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
FastStart is a trademark of Roche

sufficient for 1,250 reactions (12032945001), sufficient for 2,500 reactions (12032953001), sufficient for 250 reactions (12032929001), sufficient for 50 reactions (12032902001), sufficient for 500 reactions (12032937001)

packaging

pkg of 1,000 U (12032937001 4x250 U), pkg of 2,500 U (12032945001 10x250 U), pkg of 5,000 U (12032953001 20x250 U), pkg of 100 U (12032902001), pkg of 500 U (12032929001 2x250 U)

Fibronectin 10838039001

MDL number:

MFCD00131062

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.
Specificity
Active on most mammalian cells

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.
Testing Method
Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)
Physical form
Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.
Preparation Note
Working concentration: 5 µg/cm2
5 µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human plasma
sterility	non-sterile; 0.2 µm filtered
assay	95% (SDS-PAGE)
form	lyophilized (clear, colorless solution after reconstitution)
mol wt	440 kDa
packaging	pkg of 5 mg
Торговая марка	Roche
storage condition	avoid repeated freeze/thaw cycles
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	P02751,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... FN1(2335)

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.
Sequence
Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.
Physical form
Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin
Preparation Note
Working concentration: 5µg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Reconstitution
Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	does not flash
Flash Point C	does not flash

Fibronectin (pure)

For life science research only. Not for use in diagnostic procedures.

Quality Level	100
biological source	human plasma
sterility	non-sterile; 0.2 µm filtered
assay	>95% (SDS-PAGE)
form	lyophilized (clear, colorless solution after reconstitution)
mol wt	440 kDa
packaging	pkg of 1 mg (11051407001)","pkg of 5 mg (11080938001)
Торговая марка	Roche
application(s)	cell culture \| mammalian: suitable
UniProt accession no.	P02751,
shipped in	wet ice
storage temp.	2-8°C
Gene Information	human ... FN1(2335)

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Formamide 11814320001

Empirical Formula (Hill Notation):	CH₃NO
Linear Formula:	HCONH₂
CAS Number:	75-12-7
Molecular Weight:	45.04
Beilstein/REAXYS Number:	505995
MDL number:	MFCD00007941
PubChem Substance ID:	329749563

Formamide is an organic solvent, which allows for the denaturation and renaturation of nucleic acids at room temperature. This is particularly useful for protocols where reaction times are long and high temperatures would damage biological activity through chain scissions and depurination etc. Formamide reduces thermal stability of double stranded nucleic acids and is generally used for DNA renaturation or DNA-RNA hybridization. Specificity and rate of reaction are determined by formamide concentration and temperature of the reaction.

Formamide has been used in:
in situ hybridization

as a component of 2X denaturing loading dye for RNA polyacrylamide gel electrophoresis
for the extraction of evans blue dye in hindlimb of mice in vascular leakage assay
as a component of stop buffer used in cleavage and polyadenylation activity assays

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
Quality
Contaminants: 5 ppm heavy metals (as Pb), 5 ppm Fe

For life science research only. Not for use in diagnostic procedures.

vapor density	1.55 (vs air)
Quality Level	100,
vapor pressure	0.08 mmHg ( 20 °C), 30 mmHg ( 129 °C)
assay	99%
form	liquid
autoignition temp.	932 °F
mol wt	45.04
packaging	pkg of 500 mL
Торговая марка	Roche
application(s)	hybridization: suitable
color	colorless
refractive index	n20/D 1.447 (lit.)
pH	4-10 (20 °C, 200 g/L)
bp	210 °C (lit.)
mp	2-3 °C (lit.)
density	1.134 g/mL at 25 °C (lit.)
absorption	0.08 at 290 nm
shipped in	wet ice
storage temp.	2-8°C
SMILES string	NC=O
InChI	1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key	ZHNUHDYFZUAESO-UHFFFAOYSA-N

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	305.6 °F
Flash Point C	152 °C

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Formate Dehydrogenase

1.2.1.2 ( BRENDA | IUBMB )

Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.
Specificity
Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Most widely used in cofactor recycling systems for NADH.
Quality
Contaminants: <0.005% LDH and ADH each, <0.1% MDH
Unit Definition
One unit (U) formate dehydrogenase will oxidize 1 µmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
specific activity	~0.4 units/mg protein (At 25 °C with formate as the substrate.)
packaging	pkg of 250 U (10837016001), pkg of 80 U (10244678001)
Торговая марка	Roche
optimum pH	7.5-8.5
shipped in	wet ice
storage temp.	2-8°C