Poly[d(I-C)]

Кат. номер

11219847001

10108812001

Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
Features and Benefits
Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Quality
Typical analysis:
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length approx. 20,000 bp
In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
Unit Definition
1 A260 unit corresponds to 50 µg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 µg ds DNA.
Preparation Note
Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
For life science research only. Not for use in diagnostic procedures.

Quality Level	100,
form	lyophilized
packaging	pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])
Торговая марка	Roche
mp	54 °C (NaCl, 0.1 M, pH 7.0)
absorbance ratio	A290/260 nm 0.24-0.32, A280/260 nm 0.61-0.67, A250/260 nm 1.18-1.30
shipped in	dry ice
storage temp.	20°C

RIDADR

NONH for all modes of transport